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Protein

Ubiquitin-conjugating enzyme E2 14

Gene

UBC14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Involved in the formation of multiubiquitin chains. Signal the protein for selective degradation.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 14 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 14
TAYO29
UbcAt3
Ubiquitin carrier protein 14
Ubiquitin-protein ligase 14
Gene namesi
Name:UBC14
Synonyms:UBC3, UBC7
Ordered Locus Names:At3g55380
ORF Names:T22E16.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G55380.

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000825882 – 167Ubiquitin-conjugating enzyme E2 14Add BLAST166

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

PTM databases

iPTMnetiP42747.

Expressioni

Developmental stagei

Up-regulated during the G0 to S phase transition.1 Publication

Gene expression databases

ExpressionAtlasiP42747. baseline and differential.
GenevisibleiP42747. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi10020. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliP42747.
SMRiP42747.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233454.
InParanoidiP42747.
KOiK10575.
PhylomeDBiP42747.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P42747-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANNQASLLL QKQLKDLCKK PVDGFSAGLV DEKNVFQWSV SIMGPPDTLY
60 70 80 90 100
EGGFFNAIMS FPENYPVSPP TVTFTSEMWH PNVYSDGKVC ISILHPPGDD
110 120 130 140 150
PHGYELASER WTPVHTVESI VLSIISMLSG PNDESPANVE AAKEWRDNRA
160
EFRKKVSRCV RRSQEML
Length:167
Mass (Da):18,728
Last modified:November 1, 1995 - v1
Checksum:i15053C16A62C08BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72625 mRNA. Translation: CAA51200.1.
U33759 Genomic DNA. Translation: AAC49323.1.
DQ027028 mRNA. Translation: AAY44854.1.
AL132975 Genomic DNA. Translation: CAB75896.1.
CP002686 Genomic DNA. Translation: AEE79376.1.
AY117262 mRNA. Translation: AAM51337.1.
AY045883 mRNA. Translation: AAK76557.1.
AY086490 mRNA. Translation: AAM63492.1.
Z18513 mRNA. Translation: CAA79212.1.
PIRiS46656.
RefSeqiNP_567020.1. NM_115396.4. [P42747-1]
UniGeneiAt.206.

Genome annotation databases

EnsemblPlantsiAT3G55380.1; AT3G55380.1; AT3G55380. [P42747-1]
GeneIDi824704.
GrameneiAT3G55380.1; AT3G55380.1; AT3G55380.
KEGGiath:AT3G55380.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBC14_ARATH
AccessioniPrimary (citable) accession number: P42747
Secondary accession number(s): Q4TYZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 25, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families