P42746 (UBC3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 3
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 3
Ubiquitin-conjugating enzyme E2-17 kDa 3
Ubiquitin-protein ligase 3

Gene names

Name:	UBC3
Ordered Locus Names:	At5g62540
ORF Names:	K19B1.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Ref.2
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Expressed in all tissues examined. Lower levels found in leaves. Ref.7
Induction	Up-regulated by syringolin, a cell death-inducing chemical, but not induced by heat shock. Ref.2 Ref.7
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution	The sequence AAK82529.1 differs from that shown. Reason: Erroneous termination at position 147. Translated as Gln. The sequence AAN28744.1 differs from that shown. Reason: Erroneous termination at position 147. Translated as Gln.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from direct assay Ref.2. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from direct assay Ref.2. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 150	150	Ubiquitin-conjugating enzyme E2 3		PRO_0000082572
Sites
	Active site	88	1	Glycyl thioester intermediate By similarity
Experimental info
	Sequence conflict	13	1	F → L in AAK82529. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P42746 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 993FE149D541F2AA	FASTA	150	17,137
	10 20 30 40 50 60 MTTPAKKRLM WDFKRLQKDP PVGISGAPQD NNIMHWNALI FGPEDTPWDG GTFKLTLHFT 70 80 90 100 110 120 EDYPNKPPIV RFVSRMFHPN IYADGSICLD ILQNQWSPIY DVAAVLTSIQ SLLCDPNPDS 130 140 150 PANAEAARLF SENKREYNRK VIEIVEQSYV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana." Sullivan M.L., Carpenter T.B., Vierstra R.D. Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia. Tissue: Green leaf.
[2]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, GENE FAMILY, NOMENCLATURE.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed." Thoma S., Sullivan M.L., Vierstra R.D. Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19352 Genomic DNA. Translation: AAA32898.1. DQ027018 mRNA. Translation: AAY44844.1. AB015469 Genomic DNA. Translation: BAB11504.1. CP002688 Genomic DNA. Translation: AED97620.1. AY039851 mRNA. Translation: AAK63955.1. AY143805 mRNA. Translation: AAN28744.1. Sequence problems. AY048267 mRNA. Translation: AAK82529.1. Sequence problems. AY085367 mRNA. Translation: AAM62597.1.
IPI	IPI00525885.
PIR	S43782.
RefSeq	NP_568956.1. NM_125648.3.
UniGene	At.29044. At.332.
3D structure databases
ProteinModelPortal	P42746.
SMR	P42746. Positions 1-149.
ModBase	Search...
Proteomic databases
PaxDb	P42746.
PRIDE	P42746.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G62540.1; AT5G62540.1; AT5G62540.
GeneID	836374.
KEGG	ath:AT5G62540.
Organism-specific databases
GeneFarm	4748. 464.
TAIR	At5g62540.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233454.
InParanoid	P42746.
KO	K10573.
OMA	NIMHWNA.
PhylomeDB	P42746.
ProtClustDB	CLSN2687804.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	P42746.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC3_ARATH

Accession

Primary (citable) accession number: P42746
Secondary accession number(s): Q4TZ06, Q8H1P1, Q94AD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program