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P42746

- UBC3_ARATH

UniProt

P42746 - UBC3_ARATH

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Protein

Ubiquitin-conjugating enzyme E2 3

Gene

UBC3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G62540-MONOMER.
ReactomeiREACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 3 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 3
Ubiquitin-conjugating enzyme E2-17 kDa 3
Ubiquitin-protein ligase 3
Gene namesi
Name:UBC3
Ordered Locus Names:At5g62540
ORF Names:K19B1.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G62540.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 150150Ubiquitin-conjugating enzyme E2 3PRO_0000082572Add
BLAST

Proteomic databases

PaxDbiP42746.
PRIDEiP42746.

Expressioni

Tissue specificityi

Expressed in all tissues examined. Lower levels found in leaves.1 Publication

Inductioni

Up-regulated by syringolin, a cell death-inducing chemical, but not induced by heat shock.2 Publications

Gene expression databases

GenevestigatoriP42746.

Structurei

3D structure databases

ProteinModelPortaliP42746.
SMRiP42746. Positions 1-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233454.
InParanoidiP42746.
KOiK10573.
OMAiNGHFREP.
PhylomeDBiP42746.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42746-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTPAKKRLM WDFKRLQKDP PVGISGAPQD NNIMHWNALI FGPEDTPWDG
60 70 80 90 100
GTFKLTLHFT EDYPNKPPIV RFVSRMFHPN IYADGSICLD ILQNQWSPIY
110 120 130 140 150
DVAAVLTSIQ SLLCDPNPDS PANAEAARLF SENKREYNRK VIEIVEQSYV
Length:150
Mass (Da):17,137
Last modified:November 1, 1995 - v1
Checksum:i993FE149D541F2AA
GO

Sequence cautioni

The sequence AAK82529.1 differs from that shown. Reason: Erroneous termination at position 147. Translated as Gln.
The sequence AAN28744.1 differs from that shown. Reason: Erroneous termination at position 147. Translated as Gln.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131F → L in AAK82529. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19352 Genomic DNA. Translation: AAA32898.1.
DQ027018 mRNA. Translation: AAY44844.1.
AB015469 Genomic DNA. Translation: BAB11504.1.
CP002688 Genomic DNA. Translation: AED97620.1.
AY039851 mRNA. Translation: AAK63955.1.
AY143805 mRNA. Translation: AAN28744.1. Sequence problems.
AY048267 mRNA. Translation: AAK82529.1. Sequence problems.
AY085367 mRNA. Translation: AAM62597.1.
PIRiS43782.
RefSeqiNP_568956.1. NM_125648.3.
UniGeneiAt.29044.
At.332.

Genome annotation databases

EnsemblPlantsiAT5G62540.1; AT5G62540.1; AT5G62540.
GeneIDi836374.
KEGGiath:AT5G62540.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19352 Genomic DNA. Translation: AAA32898.1 .
DQ027018 mRNA. Translation: AAY44844.1 .
AB015469 Genomic DNA. Translation: BAB11504.1 .
CP002688 Genomic DNA. Translation: AED97620.1 .
AY039851 mRNA. Translation: AAK63955.1 .
AY143805 mRNA. Translation: AAN28744.1 . Sequence problems.
AY048267 mRNA. Translation: AAK82529.1 . Sequence problems.
AY085367 mRNA. Translation: AAM62597.1 .
PIRi S43782.
RefSeqi NP_568956.1. NM_125648.3.
UniGenei At.29044.
At.332.

3D structure databases

ProteinModelPortali P42746.
SMRi P42746. Positions 1-149.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P42746.
PRIDEi P42746.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G62540.1 ; AT5G62540.1 ; AT5G62540 .
GeneIDi 836374.
KEGGi ath:AT5G62540.

Organism-specific databases

GeneFarmi 4748. 464.
TAIRi AT5G62540.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233454.
InParanoidi P42746.
KOi K10573.
OMAi NGHFREP.
PhylomeDBi P42746.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT5G62540-MONOMER.
Reactomei REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.

Gene expression databases

Genevestigatori P42746.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana."
    Sullivan M.L., Carpenter T.B., Vierstra R.D.
    Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
    Tissue: Green leaf.
  2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, GENE FAMILY, NOMENCLATURE.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
    Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed."
    Thoma S., Sullivan M.L., Vierstra R.D.
    Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiUBC3_ARATH
AccessioniPrimary (citable) accession number: P42746
Secondary accession number(s): Q4TZ06, Q8H1P1, Q94AD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 1, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3