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P42746 (UBC3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 3

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein 3
Ubiquitin-conjugating enzyme E2-17 kDa 3
Ubiquitin-protein ligase 3
Gene names
Name:UBC3
Ordered Locus Names:At5g62540
ORF Names:K19B1.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length150 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Ref.2

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Tissue specificity

Expressed in all tissues examined. Lower levels found in leaves. Ref.7

Induction

Up-regulated by syringolin, a cell death-inducing chemical, but not induced by heat shock. Ref.2 Ref.7

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence caution

The sequence AAK82529.1 differs from that shown. Reason: Erroneous termination at position 147. Translated as Gln.

The sequence AAN28744.1 differs from that shown. Reason: Erroneous termination at position 147. Translated as Gln.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from direct assay Ref.2. Source: TAIR

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein transferase activity

Inferred from direct assay Ref.2. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 150150Ubiquitin-conjugating enzyme E2 3
PRO_0000082572

Sites

Active site881Glycyl thioester intermediate By similarity

Experimental info

Sequence conflict131F → L in AAK82529. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P42746 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 993FE149D541F2AA

FASTA15017,137
        10         20         30         40         50         60 
MTTPAKKRLM WDFKRLQKDP PVGISGAPQD NNIMHWNALI FGPEDTPWDG GTFKLTLHFT 

        70         80         90        100        110        120 
EDYPNKPPIV RFVSRMFHPN IYADGSICLD ILQNQWSPIY DVAAVLTSIQ SLLCDPNPDS 

       130        140        150 
PANAEAARLF SENKREYNRK VIEIVEQSYV 

« Hide

References

« Hide 'large scale' references
[1]"Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana."
Sullivan M.L., Carpenter T.B., Vierstra R.D.
Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
Tissue: Green leaf.
[2]"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, GENE FAMILY, NOMENCLATURE.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed."
Thoma S., Sullivan M.L., Vierstra R.D.
Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
+Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L19352 Genomic DNA. Translation: AAA32898.1.
DQ027018 mRNA. Translation: AAY44844.1.
AB015469 Genomic DNA. Translation: BAB11504.1.
CP002688 Genomic DNA. Translation: AED97620.1.
AY039851 mRNA. Translation: AAK63955.1.
AY143805 mRNA. Translation: AAN28744.1. Sequence problems.
AY048267 mRNA. Translation: AAK82529.1. Sequence problems.
AY085367 mRNA. Translation: AAM62597.1.
PIRS43782.
RefSeqNP_568956.1. NM_125648.3.
UniGeneAt.29044.
At.332.

3D structure databases

ProteinModelPortalP42746.
SMRP42746. Positions 1-149.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP42746.
PRIDEP42746.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G62540.1; AT5G62540.1; AT5G62540.
GeneID836374.
KEGGath:AT5G62540.

Organism-specific databases

GeneFarm4748. 464.
TAIRAT5G62540.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000233454.
InParanoidP42746.
KOK10573.
OMANGHFREP.
PhylomeDBP42746.

Enzyme and pathway databases

BioCycARA:AT5G62540-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorP42746.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC3_ARATH
AccessionPrimary (citable) accession number: P42746
Secondary accession number(s): Q4TZ06, Q8H1P1, Q94AD2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names