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Protein

Ubiquitin-conjugating enzyme E2 3

Gene

UBC3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G62540-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 3 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 3
Ubiquitin-conjugating enzyme E2-17 kDa 3
Ubiquitin-protein ligase 3
Gene namesi
Name:UBC3
Ordered Locus Names:At5g62540
ORF Names:K19B1.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G62540.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 150150Ubiquitin-conjugating enzyme E2 3PRO_0000082572Add
BLAST

Proteomic databases

PaxDbiP42746.
PRIDEiP42746.

Expressioni

Tissue specificityi

Expressed in all tissues examined. Lower levels found in leaves.1 Publication

Inductioni

Up-regulated by syringolin, a cell death-inducing chemical, but not induced by heat shock.2 Publications

Gene expression databases

GenevestigatoriP42746.

Structurei

3D structure databases

ProteinModelPortaliP42746.
SMRiP42746. Positions 1-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233454.
InParanoidiP42746.
KOiK10573.
OMAiNXSLLDE.
PhylomeDBiP42746.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42746-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPAKKRLM WDFKRLQKDP PVGISGAPQD NNIMHWNALI FGPEDTPWDG
60 70 80 90 100
GTFKLTLHFT EDYPNKPPIV RFVSRMFHPN IYADGSICLD ILQNQWSPIY
110 120 130 140 150
DVAAVLTSIQ SLLCDPNPDS PANAEAARLF SENKREYNRK VIEIVEQSYV
Length:150
Mass (Da):17,137
Last modified:November 1, 1995 - v1
Checksum:i993FE149D541F2AA
GO

Sequence cautioni

The sequence AAK82529.1 differs from that shown. Reason: Erroneous termination at position 147. Translated as Gln.Curated
The sequence AAN28744.1 differs from that shown. Reason: Erroneous termination at position 147. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131F → L in AAK82529 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19352 Genomic DNA. Translation: AAA32898.1.
DQ027018 mRNA. Translation: AAY44844.1.
AB015469 Genomic DNA. Translation: BAB11504.1.
CP002688 Genomic DNA. Translation: AED97620.1.
AY039851 mRNA. Translation: AAK63955.1.
AY143805 mRNA. Translation: AAN28744.1. Sequence problems.
AY048267 mRNA. Translation: AAK82529.1. Sequence problems.
AY085367 mRNA. Translation: AAM62597.1.
PIRiS43782.
RefSeqiNP_568956.1. NM_125648.3.
UniGeneiAt.29044.
At.332.

Genome annotation databases

EnsemblPlantsiAT5G62540.1; AT5G62540.1; AT5G62540.
GeneIDi836374.
KEGGiath:AT5G62540.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19352 Genomic DNA. Translation: AAA32898.1.
DQ027018 mRNA. Translation: AAY44844.1.
AB015469 Genomic DNA. Translation: BAB11504.1.
CP002688 Genomic DNA. Translation: AED97620.1.
AY039851 mRNA. Translation: AAK63955.1.
AY143805 mRNA. Translation: AAN28744.1. Sequence problems.
AY048267 mRNA. Translation: AAK82529.1. Sequence problems.
AY085367 mRNA. Translation: AAM62597.1.
PIRiS43782.
RefSeqiNP_568956.1. NM_125648.3.
UniGeneiAt.29044.
At.332.

3D structure databases

ProteinModelPortaliP42746.
SMRiP42746. Positions 1-149.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP42746.
PRIDEiP42746.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G62540.1; AT5G62540.1; AT5G62540.
GeneIDi836374.
KEGGiath:AT5G62540.

Organism-specific databases

GeneFarmi4748. 464.
TAIRiAT5G62540.

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233454.
InParanoidiP42746.
KOiK10573.
OMAiNXSLLDE.
PhylomeDBiP42746.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciARA:AT5G62540-MONOMER.

Miscellaneous databases

PROiP42746.

Gene expression databases

GenevestigatoriP42746.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana."
    Sullivan M.L., Carpenter T.B., Vierstra R.D.
    Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
    Tissue: Green leaf.
  2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, GENE FAMILY, NOMENCLATURE.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
    Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed."
    Thoma S., Sullivan M.L., Vierstra R.D.
    Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiUBC3_ARATH
AccessioniPrimary (citable) accession number: P42746
Secondary accession number(s): Q4TZ06, Q8H1P1, Q94AD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 29, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.