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P42746

- UBC3_ARATH

UniProt

P42746 - UBC3_ARATH

Protein

Ubiquitin-conjugating enzyme E2 3

Gene

UBC3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G62540-MONOMER.
    ReactomeiREACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 3 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 3
    Ubiquitin-conjugating enzyme E2-17 kDa 3
    Ubiquitin-protein ligase 3
    Gene namesi
    Name:UBC3
    Ordered Locus Names:At5g62540
    ORF Names:K19B1.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G62540.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 150150Ubiquitin-conjugating enzyme E2 3PRO_0000082572Add
    BLAST

    Proteomic databases

    PaxDbiP42746.
    PRIDEiP42746.

    Expressioni

    Tissue specificityi

    Expressed in all tissues examined. Lower levels found in leaves.1 Publication

    Inductioni

    Up-regulated by syringolin, a cell death-inducing chemical, but not induced by heat shock.2 Publications

    Gene expression databases

    GenevestigatoriP42746.

    Structurei

    3D structure databases

    ProteinModelPortaliP42746.
    SMRiP42746. Positions 1-149.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233454.
    InParanoidiP42746.
    KOiK10573.
    OMAiNGHFREP.
    PhylomeDBiP42746.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42746-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTPAKKRLM WDFKRLQKDP PVGISGAPQD NNIMHWNALI FGPEDTPWDG    50
    GTFKLTLHFT EDYPNKPPIV RFVSRMFHPN IYADGSICLD ILQNQWSPIY 100
    DVAAVLTSIQ SLLCDPNPDS PANAEAARLF SENKREYNRK VIEIVEQSYV 150
    Length:150
    Mass (Da):17,137
    Last modified:November 1, 1995 - v1
    Checksum:i993FE149D541F2AA
    GO

    Sequence cautioni

    The sequence AAK82529.1 differs from that shown. Reason: Erroneous termination at position 147. Translated as Gln.
    The sequence AAN28744.1 differs from that shown. Reason: Erroneous termination at position 147. Translated as Gln.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131F → L in AAK82529. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19352 Genomic DNA. Translation: AAA32898.1.
    DQ027018 mRNA. Translation: AAY44844.1.
    AB015469 Genomic DNA. Translation: BAB11504.1.
    CP002688 Genomic DNA. Translation: AED97620.1.
    AY039851 mRNA. Translation: AAK63955.1.
    AY143805 mRNA. Translation: AAN28744.1. Sequence problems.
    AY048267 mRNA. Translation: AAK82529.1. Sequence problems.
    AY085367 mRNA. Translation: AAM62597.1.
    PIRiS43782.
    RefSeqiNP_568956.1. NM_125648.3.
    UniGeneiAt.29044.
    At.332.

    Genome annotation databases

    EnsemblPlantsiAT5G62540.1; AT5G62540.1; AT5G62540.
    GeneIDi836374.
    KEGGiath:AT5G62540.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19352 Genomic DNA. Translation: AAA32898.1 .
    DQ027018 mRNA. Translation: AAY44844.1 .
    AB015469 Genomic DNA. Translation: BAB11504.1 .
    CP002688 Genomic DNA. Translation: AED97620.1 .
    AY039851 mRNA. Translation: AAK63955.1 .
    AY143805 mRNA. Translation: AAN28744.1 . Sequence problems.
    AY048267 mRNA. Translation: AAK82529.1 . Sequence problems.
    AY085367 mRNA. Translation: AAM62597.1 .
    PIRi S43782.
    RefSeqi NP_568956.1. NM_125648.3.
    UniGenei At.29044.
    At.332.

    3D structure databases

    ProteinModelPortali P42746.
    SMRi P42746. Positions 1-149.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P42746.
    PRIDEi P42746.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G62540.1 ; AT5G62540.1 ; AT5G62540 .
    GeneIDi 836374.
    KEGGi ath:AT5G62540.

    Organism-specific databases

    GeneFarmi 4748. 464.
    TAIRi AT5G62540.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233454.
    InParanoidi P42746.
    KOi K10573.
    OMAi NGHFREP.
    PhylomeDBi P42746.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT5G62540-MONOMER.
    Reactomei REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.

    Gene expression databases

    Genevestigatori P42746.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana."
      Sullivan M.L., Carpenter T.B., Vierstra R.D.
      Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
      Tissue: Green leaf.
    2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, GENE FAMILY, NOMENCLATURE.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
      Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
      DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. "Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed."
      Thoma S., Sullivan M.L., Vierstra R.D.
      Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiUBC3_ARATH
    AccessioniPrimary (citable) accession number: P42746
    Secondary accession number(s): Q4TZ06, Q8H1P1, Q94AD2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3