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P42745

- UBC2_ARATH

UniProt

P42745 - UBC2_ARATH

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Protein

Ubiquitin-conjugating enzyme E2 2

Gene

UBC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. histone H2B ubiquitination Source: TAIR
  2. ubiquitin-dependent protein catabolic process Source: TAIR
  3. UV protection Source: TAIR
  4. vegetative to reproductive phase transition of meristem Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G02760-MONOMER.
ReactomeiREACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 2 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 2
Ubiquitin-conjugating enzyme E2-17 kDa 2
Ubiquitin-protein ligase 2
Gene namesi
Name:UBC2
Ordered Locus Names:At2g02760
ORF Names:T20F6.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G02760.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Ubiquitin-conjugating enzyme E2 2PRO_0000082571Add
BLAST

Proteomic databases

PaxDbiP42745.
PRIDEiP42745.

Expressioni

Tissue specificityi

Expressed in all tissues examined. Lower levels found in leaves.1 Publication

Inductioni

Not induced by heat shock.1 Publication

Gene expression databases

ExpressionAtlasiP42745. baseline and differential.
GenevestigatoriP42745.

Interactioni

Protein-protein interaction databases

BioGridi207. 1 interaction.
IntActiP42745. 2 interactions.
STRINGi3702.AT2G02760.1-P.

Structurei

3D structure databases

ProteinModelPortaliP42745.
SMRiP42745. Positions 1-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233454.
InParanoidiP42745.
KOiK10573.
OMAiIVEQSWV.
PhylomeDBiP42745.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42745-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTPARKRLM RDFKRLQQDP PAGISGAPQD NNIMLWNAVI FGPDDTPWDG
60 70 80 90 100
GTFKLSLQFS EDYPNKPPTV RFVSRMFHPN IYADGSICLD ILQNQWSPIY
110 120 130 140 150
DVAAILTSIQ SLLCDPNPNS PANSEAARMF SESKREYNRR VREVVEQSWT

AD
Length:152
Mass (Da):17,279
Last modified:November 1, 1995 - v1
Checksum:i266CD41930C38629
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19353 Genomic DNA. Translation: AAA32899.1.
Y13031 mRNA. Translation: CAA73476.1.
DQ027017 mRNA. Translation: AAY44843.1.
AC002521 Genomic DNA. Translation: AAC05346.1.
CP002685 Genomic DNA. Translation: AEC05621.1.
AF370558 mRNA. Translation: AAK48985.1.
AY072479 mRNA. Translation: AAL66894.1.
PIRiS43783.
RefSeqiNP_565289.1. NM_126331.3.
UniGeneiAt.203.

Genome annotation databases

EnsemblPlantsiAT2G02760.1; AT2G02760.1; AT2G02760.
GeneIDi814805.
KEGGiath:AT2G02760.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19353 Genomic DNA. Translation: AAA32899.1 .
Y13031 mRNA. Translation: CAA73476.1 .
DQ027017 mRNA. Translation: AAY44843.1 .
AC002521 Genomic DNA. Translation: AAC05346.1 .
CP002685 Genomic DNA. Translation: AEC05621.1 .
AF370558 mRNA. Translation: AAK48985.1 .
AY072479 mRNA. Translation: AAL66894.1 .
PIRi S43783.
RefSeqi NP_565289.1. NM_126331.3.
UniGenei At.203.

3D structure databases

ProteinModelPortali P42745.
SMRi P42745. Positions 1-150.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207. 1 interaction.
IntActi P42745. 2 interactions.
STRINGi 3702.AT2G02760.1-P.

Proteomic databases

PaxDbi P42745.
PRIDEi P42745.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G02760.1 ; AT2G02760.1 ; AT2G02760 .
GeneIDi 814805.
KEGGi ath:AT2G02760.

Organism-specific databases

GeneFarmi 4744. 464.
TAIRi AT2G02760.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233454.
InParanoidi P42745.
KOi K10573.
OMAi IVEQSWV.
PhylomeDBi P42745.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT2G02760-MONOMER.
Reactomei REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.

Gene expression databases

ExpressionAtlasi P42745. baseline and differential.
Genevestigatori P42745.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana."
    Sullivan M.L., Carpenter T.B., Vierstra R.D.
    Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
    Tissue: Green leaf.
  2. "Arabidopsis thaliana RAD6 homolog AtUBC2 complements UV sensitivity, but not N-end rule degradation deficiency, of Saccharomyces cerevisiae rad6 mutants."
    Zwirn P., Stary S., Luschnig C., Bachmair A.
    Curr. Genet. 32:309-314(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GENE FAMILY, NOMENCLATURE.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed."
    Thoma S., Sullivan M.L., Vierstra R.D.
    Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiUBC2_ARATH
AccessioniPrimary (citable) accession number: P42745
Secondary accession number(s): Q4TZ07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3