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P42745

- UBC2_ARATH

UniProt

P42745 - UBC2_ARATH

Protein

Ubiquitin-conjugating enzyme E2 2

Gene

UBC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. histone H2B ubiquitination Source: TAIR
    2. ubiquitin-dependent protein catabolic process Source: TAIR
    3. UV protection Source: TAIR
    4. vegetative to reproductive phase transition of meristem Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G02760-MONOMER.
    ReactomeiREACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 2 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 2
    Ubiquitin-conjugating enzyme E2-17 kDa 2
    Ubiquitin-protein ligase 2
    Gene namesi
    Name:UBC2
    Ordered Locus Names:At2g02760
    ORF Names:T20F6.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G02760.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 152152Ubiquitin-conjugating enzyme E2 2PRO_0000082571Add
    BLAST

    Proteomic databases

    PaxDbiP42745.
    PRIDEiP42745.

    Expressioni

    Tissue specificityi

    Expressed in all tissues examined. Lower levels found in leaves.1 Publication

    Inductioni

    Not induced by heat shock.1 Publication

    Gene expression databases

    ArrayExpressiP42745.
    GenevestigatoriP42745.

    Interactioni

    Protein-protein interaction databases

    BioGridi207. 1 interaction.
    IntActiP42745. 2 interactions.
    STRINGi3702.AT2G02760.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP42745.
    SMRiP42745. Positions 1-150.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233454.
    InParanoidiP42745.
    KOiK10573.
    OMAiIVEQSWV.
    PhylomeDBiP42745.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42745-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTPARKRLM RDFKRLQQDP PAGISGAPQD NNIMLWNAVI FGPDDTPWDG    50
    GTFKLSLQFS EDYPNKPPTV RFVSRMFHPN IYADGSICLD ILQNQWSPIY 100
    DVAAILTSIQ SLLCDPNPNS PANSEAARMF SESKREYNRR VREVVEQSWT 150
    AD 152
    Length:152
    Mass (Da):17,279
    Last modified:November 1, 1995 - v1
    Checksum:i266CD41930C38629
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19353 Genomic DNA. Translation: AAA32899.1.
    Y13031 mRNA. Translation: CAA73476.1.
    DQ027017 mRNA. Translation: AAY44843.1.
    AC002521 Genomic DNA. Translation: AAC05346.1.
    CP002685 Genomic DNA. Translation: AEC05621.1.
    AF370558 mRNA. Translation: AAK48985.1.
    AY072479 mRNA. Translation: AAL66894.1.
    PIRiS43783.
    RefSeqiNP_565289.1. NM_126331.3.
    UniGeneiAt.203.

    Genome annotation databases

    EnsemblPlantsiAT2G02760.1; AT2G02760.1; AT2G02760.
    GeneIDi814805.
    KEGGiath:AT2G02760.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19353 Genomic DNA. Translation: AAA32899.1 .
    Y13031 mRNA. Translation: CAA73476.1 .
    DQ027017 mRNA. Translation: AAY44843.1 .
    AC002521 Genomic DNA. Translation: AAC05346.1 .
    CP002685 Genomic DNA. Translation: AEC05621.1 .
    AF370558 mRNA. Translation: AAK48985.1 .
    AY072479 mRNA. Translation: AAL66894.1 .
    PIRi S43783.
    RefSeqi NP_565289.1. NM_126331.3.
    UniGenei At.203.

    3D structure databases

    ProteinModelPortali P42745.
    SMRi P42745. Positions 1-150.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207. 1 interaction.
    IntActi P42745. 2 interactions.
    STRINGi 3702.AT2G02760.1-P.

    Proteomic databases

    PaxDbi P42745.
    PRIDEi P42745.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G02760.1 ; AT2G02760.1 ; AT2G02760 .
    GeneIDi 814805.
    KEGGi ath:AT2G02760.

    Organism-specific databases

    GeneFarmi 4744. 464.
    TAIRi AT2G02760.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233454.
    InParanoidi P42745.
    KOi K10573.
    OMAi IVEQSWV.
    PhylomeDBi P42745.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT2G02760-MONOMER.
    Reactomei REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.

    Gene expression databases

    ArrayExpressi P42745.
    Genevestigatori P42745.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana."
      Sullivan M.L., Carpenter T.B., Vierstra R.D.
      Plant Mol. Biol. 24:651-661(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
      Tissue: Green leaf.
    2. "Arabidopsis thaliana RAD6 homolog AtUBC2 complements UV sensitivity, but not N-end rule degradation deficiency, of Saccharomyces cerevisiae rad6 mutants."
      Zwirn P., Stary S., Luschnig C., Bachmair A.
      Curr. Genet. 32:309-314(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    3. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GENE FAMILY, NOMENCLATURE.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed."
      Thoma S., Sullivan M.L., Vierstra R.D.
      Plant Mol. Biol. 31:493-505(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiUBC2_ARATH
    AccessioniPrimary (citable) accession number: P42745
    Secondary accession number(s): Q4TZ07
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3