Reviewed, UniProtKB/Swiss-Prot P42743 (UBC15_ARATH)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme 15
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-conjugating enzyme E2-18 kDa 15
    Ubiquitin-protein ligase
    Ubiquitin carrier protein
    PM42

Gene names

Name:	UBC15
Synonyms:	UBC2-1
Ordered Locus Names:	At1g45050
ORF Names:	F27F5.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Ref.1
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution	The sequence AAF69157.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leaf morphogenesis Inferred from genetic interaction. Source: TAIR negative regulation of flower development Inferred from genetic interaction. Source: TAIR post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro ubiquitin-dependent protein catabolic process Ref.3 Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Ref.3 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	Ubiquitin-conjugating enzyme 15		PRO_0000082575
Sites
	Active site	99	1	Glycyl thioester intermediate By similarity
Experimental info
	Sequence conflict	33	1	T → S in CAA48378. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P42743-1 [UniParc]. Last modified December 6, 2002. Version 2. Checksum: 60C7F34A9574FB68	FASTA	161	18,262
	10 20 30 40 50 60 MTSSSAPSRK ALSKIACNRL QKELSEWQLN PPTGFRHKVT DNLQKWTIDV TGAPGTLYAN 70 80 90 100 110 120 ETYQLQVEFP EHYPMEAPQV VFVSPAPSHP HIYSNGHICL DILYDSWSPA MTVNSVCISI 130 140 150 160 LSMLSSSPAK QRPADNDRYV KNCKNGRSPK ETRWWFHDDK V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional expression and molecular characterization of AtUBC2-1, a novel ubiquitin-conjugating enzyme (E2) from Arabidopsis thaliana." Bartling D., Rehling P., Weiler E.W. Plant Mol. Biol. 23:387-396(1993) [PubMed: 8219072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: cv. Landsberg erecta. Tissue: Leaf.
[2]	"A new family of ubiquitin-conjugating enzymes (E2S) AtUBC15/16/17/18 in Arabidopsis thaliana." Yan N., Doelling J., Vierstra R.D. Plant Gene Register PGR97-174 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed: 16339806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
[4]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Arabidopsis ORF clones." Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68306 mRNA. Translation: CAA48378.1. AF028338 Genomic DNA. Translation: AAC39324.1. DQ027029 mRNA. Translation: AAY44855.1. AC007915 Genomic DNA. Translation: AAF69157.1. Sequence problems. BT026105 mRNA. Translation: ABG48461.1. AK176757 mRNA. Translation: BAD44520.1.
IPI	IPI00538643.
PIR	C96509. S39483.
RefSeq	NP_564493.1.
UniGene	At.204 Rsa.7518
3D structure databases
SMR	P42743. Positions 13-144.
ModBase	Search...
Protein-protein interaction databases
STRING	P42743.
Genome annotation databases
GeneID	841072.
GenomeReviews	Gene locus AT1G45050 in contig CT485782_GR.
KEGG	ath:AT1G45050.
Organism-specific databases
TAIR	At1g45050.
Phylogenomic databases
eggNOG	KOG0427.
HOGENOM	HBG756483.
InParanoid	P42743.
OMA	NSVCISI.
PhylomeDB	P42743.
Enzyme and pathway databases
BRENDA	6.3.2.19. 302.
Gene expression databases
ArrayExpress	P42743.
Genevestigator	P42743.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC15_ARATH

Accession

Primary (citable) accession number: P42743
Secondary accession number(s): O48952, Q67XR1, Q9MAK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 6, 2002
Last modified:	February 9, 2010
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)