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Protein

Ubiquitin-conjugating enzyme 15

Gene

UBC15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei99 – 991Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G45050-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme 15 (EC:6.3.2.19)
Alternative name(s):
PM42
Ubiquitin carrier protein
Ubiquitin-conjugating enzyme E2-18 kDa 15
Ubiquitin-protein ligase
Gene namesi
Name:UBC15
Synonyms:UBC2-1
Ordered Locus Names:At1g45050
ORF Names:F27F5.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G45050.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161Ubiquitin-conjugating enzyme 15PRO_0000082575Add
BLAST

Proteomic databases

PaxDbiP42743.
PRIDEiP42743.

Expressioni

Gene expression databases

GenevestigatoriP42743.

Structurei

3D structure databases

ProteinModelPortaliP42743.
SMRiP42743. Positions 19-154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233453.
InParanoidiP42743.
KOiK10688.
OMAiSDNLEEW.
PhylomeDBiP42743.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42743-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSSSAPSRK ALSKIACNRL QKELSEWQLN PPTGFRHKVT DNLQKWTIDV
60 70 80 90 100
TGAPGTLYAN ETYQLQVEFP EHYPMEAPQV VFVSPAPSHP HIYSNGHICL
110 120 130 140 150
DILYDSWSPA MTVNSVCISI LSMLSSSPAK QRPADNDRYV KNCKNGRSPK
160
ETRWWFHDDK V
Length:161
Mass (Da):18,262
Last modified:December 6, 2002 - v2
Checksum:i60C7F34A9574FB68
GO

Sequence cautioni

The sequence AAF69157.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331T → S in CAA48378 (PubMed:8219072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68306 mRNA. Translation: CAA48378.1.
AF028338 Genomic DNA. Translation: AAC39324.1.
DQ027029 mRNA. Translation: AAY44855.1.
AC007915 Genomic DNA. Translation: AAF69157.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32077.1.
BT026105 mRNA. Translation: ABG48461.1.
AK176757 mRNA. Translation: BAD44520.1.
PIRiC96509.
S39483.
RefSeqiNP_564493.1. NM_103582.3.
UniGeneiAt.204.

Genome annotation databases

EnsemblPlantsiAT1G45050.1; AT1G45050.1; AT1G45050.
GeneIDi841072.
KEGGiath:AT1G45050.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68306 mRNA. Translation: CAA48378.1.
AF028338 Genomic DNA. Translation: AAC39324.1.
DQ027029 mRNA. Translation: AAY44855.1.
AC007915 Genomic DNA. Translation: AAF69157.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32077.1.
BT026105 mRNA. Translation: ABG48461.1.
AK176757 mRNA. Translation: BAD44520.1.
PIRiC96509.
S39483.
RefSeqiNP_564493.1. NM_103582.3.
UniGeneiAt.204.

3D structure databases

ProteinModelPortaliP42743.
SMRiP42743. Positions 19-154.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP42743.
PRIDEiP42743.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G45050.1; AT1G45050.1; AT1G45050.
GeneIDi841072.
KEGGiath:AT1G45050.

Organism-specific databases

TAIRiAT1G45050.

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233453.
InParanoidiP42743.
KOiK10688.
OMAiSDNLEEW.
PhylomeDBiP42743.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciARA:AT1G45050-MONOMER.

Miscellaneous databases

PROiP42743.

Gene expression databases

GenevestigatoriP42743.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Functional expression and molecular characterization of AtUBC2-1, a novel ubiquitin-conjugating enzyme (E2) from Arabidopsis thaliana."
    Bartling D., Rehling P., Weiler E.W.
    Plant Mol. Biol. 23:387-396(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: cv. Landsberg erecta.
    Tissue: Leaf.
  2. "A new family of ubiquitin-conjugating enzymes (E2S) AtUBC15/16/17/18 in Arabidopsis thaliana."
    Yan N., Doelling J., Vierstra R.D.
    Plant Gene Register PGR97-174
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
  4. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBC15_ARATH
AccessioniPrimary (citable) accession number: P42743
Secondary accession number(s): O48952, Q67XR1, Q9MAK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 6, 2002
Last modified: May 27, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.