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P42743 (UBC15_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme 15

EC=6.3.2.19
Alternative name(s):
PM42
Ubiquitin carrier protein
Ubiquitin-conjugating enzyme E2-18 kDa 15
Ubiquitin-protein ligase
Gene names
Name:UBC15
Synonyms:UBC2-1
Ordered Locus Names:At1g45050
ORF Names:F27F5.13
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length161 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Ref.1

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence caution

The sequence AAF69157.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 161161Ubiquitin-conjugating enzyme 15
PRO_0000082575

Sites

Active site991Glycyl thioester intermediate By similarity

Experimental info

Sequence conflict331T → S in CAA48378. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P42743 [UniParc].

Last modified December 6, 2002. Version 2.
Checksum: 60C7F34A9574FB68

FASTA16118,262
        10         20         30         40         50         60 
MTSSSAPSRK ALSKIACNRL QKELSEWQLN PPTGFRHKVT DNLQKWTIDV TGAPGTLYAN 

        70         80         90        100        110        120 
ETYQLQVEFP EHYPMEAPQV VFVSPAPSHP HIYSNGHICL DILYDSWSPA MTVNSVCISI 

       130        140        150        160 
LSMLSSSPAK QRPADNDRYV KNCKNGRSPK ETRWWFHDDK V 

« Hide

References

« Hide 'large scale' references
[1]"Functional expression and molecular characterization of AtUBC2-1, a novel ubiquitin-conjugating enzyme (E2) from Arabidopsis thaliana."
Bartling D., Rehling P., Weiler E.W.
Plant Mol. Biol. 23:387-396(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Strain: cv. Landsberg erecta.
Tissue: Leaf.
[2]"A new family of ubiquitin-conjugating enzymes (E2S) AtUBC15/16/17/18 in Arabidopsis thaliana."
Yan N., Doelling J., Vierstra R.D.
Plant Gene Register PGR97-174
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[3]"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
[4]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Arabidopsis ORF clones."
Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X68306 mRNA. Translation: CAA48378.1.
AF028338 Genomic DNA. Translation: AAC39324.1.
DQ027029 mRNA. Translation: AAY44855.1.
AC007915 Genomic DNA. Translation: AAF69157.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32077.1.
BT026105 mRNA. Translation: ABG48461.1.
AK176757 mRNA. Translation: BAD44520.1.
PIRC96509.
S39483.
RefSeqNP_564493.1. NM_103582.3.
UniGeneAt.204.

3D structure databases

ProteinModelPortalP42743.
SMRP42743. Positions 19-154.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP42743.
PRIDEP42743.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G45050.1; AT1G45050.1; AT1G45050.
GeneID841072.
KEGGath:AT1G45050.

Organism-specific databases

TAIRAT1G45050.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000233453.
InParanoidP42743.
KOK10688.
OMAFQIRIAN.
PhylomeDBP42743.
ProtClustDBCLSN2688481.

Enzyme and pathway databases

BioCycARA:AT1G45050-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorP42743.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC15_ARATH
AccessionPrimary (citable) accession number: P42743
Secondary accession number(s): O48952, Q67XR1, Q9MAK4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 6, 2002
Last modified: March 19, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names