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P42743

- UBC15_ARATH

UniProt

P42743 - UBC15_ARATH

Protein

Ubiquitin-conjugating enzyme 15

Gene

UBC15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (06 Dec 2002)
      Previous versions | rss
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    Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei99 – 991Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. leaf morphogenesis Source: TAIR
    2. negative regulation of flower development Source: TAIR
    3. ubiquitin-dependent protein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G45050-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme 15 (EC:6.3.2.19)
    Alternative name(s):
    PM42
    Ubiquitin carrier protein
    Ubiquitin-conjugating enzyme E2-18 kDa 15
    Ubiquitin-protein ligase
    Gene namesi
    Name:UBC15
    Synonyms:UBC2-1
    Ordered Locus Names:At1g45050
    ORF Names:F27F5.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G45050.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 161161Ubiquitin-conjugating enzyme 15PRO_0000082575Add
    BLAST

    Proteomic databases

    PaxDbiP42743.
    PRIDEiP42743.

    Expressioni

    Gene expression databases

    GenevestigatoriP42743.

    Structurei

    3D structure databases

    ProteinModelPortaliP42743.
    SMRiP42743. Positions 19-125.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233453.
    InParanoidiP42743.
    KOiK10688.
    OMAiWIFTIEV.
    PhylomeDBiP42743.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42743-1 [UniParc]FASTAAdd to Basket

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    MTSSSAPSRK ALSKIACNRL QKELSEWQLN PPTGFRHKVT DNLQKWTIDV    50
    TGAPGTLYAN ETYQLQVEFP EHYPMEAPQV VFVSPAPSHP HIYSNGHICL 100
    DILYDSWSPA MTVNSVCISI LSMLSSSPAK QRPADNDRYV KNCKNGRSPK 150
    ETRWWFHDDK V 161
    Length:161
    Mass (Da):18,262
    Last modified:December 6, 2002 - v2
    Checksum:i60C7F34A9574FB68
    GO

    Sequence cautioni

    The sequence AAF69157.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti33 – 331T → S in CAA48378. (PubMed:8219072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68306 mRNA. Translation: CAA48378.1.
    AF028338 Genomic DNA. Translation: AAC39324.1.
    DQ027029 mRNA. Translation: AAY44855.1.
    AC007915 Genomic DNA. Translation: AAF69157.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32077.1.
    BT026105 mRNA. Translation: ABG48461.1.
    AK176757 mRNA. Translation: BAD44520.1.
    PIRiC96509.
    S39483.
    RefSeqiNP_564493.1. NM_103582.3.
    UniGeneiAt.204.

    Genome annotation databases

    EnsemblPlantsiAT1G45050.1; AT1G45050.1; AT1G45050.
    GeneIDi841072.
    KEGGiath:AT1G45050.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68306 mRNA. Translation: CAA48378.1 .
    AF028338 Genomic DNA. Translation: AAC39324.1 .
    DQ027029 mRNA. Translation: AAY44855.1 .
    AC007915 Genomic DNA. Translation: AAF69157.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32077.1 .
    BT026105 mRNA. Translation: ABG48461.1 .
    AK176757 mRNA. Translation: BAD44520.1 .
    PIRi C96509.
    S39483.
    RefSeqi NP_564493.1. NM_103582.3.
    UniGenei At.204.

    3D structure databases

    ProteinModelPortali P42743.
    SMRi P42743. Positions 19-125.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P42743.
    PRIDEi P42743.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G45050.1 ; AT1G45050.1 ; AT1G45050 .
    GeneIDi 841072.
    KEGGi ath:AT1G45050.

    Organism-specific databases

    TAIRi AT1G45050.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233453.
    InParanoidi P42743.
    KOi K10688.
    OMAi WIFTIEV.
    PhylomeDBi P42743.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT1G45050-MONOMER.

    Gene expression databases

    Genevestigatori P42743.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional expression and molecular characterization of AtUBC2-1, a novel ubiquitin-conjugating enzyme (E2) from Arabidopsis thaliana."
      Bartling D., Rehling P., Weiler E.W.
      Plant Mol. Biol. 23:387-396(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Strain: cv. Landsberg erecta.
      Tissue: Leaf.
    2. "A new family of ubiquitin-conjugating enzymes (E2S) AtUBC15/16/17/18 in Arabidopsis thaliana."
      Yan N., Doelling J., Vierstra R.D.
      Plant Gene Register PGR97-174
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    3. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
    4. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Arabidopsis ORF clones."
      Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBC15_ARATH
    AccessioniPrimary (citable) accession number: P42743
    Secondary accession number(s): O48952, Q67XR1, Q9MAK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: December 6, 2002
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3