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P42743

- UBC15_ARATH

UniProt

P42743 - UBC15_ARATH

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Protein

Ubiquitin-conjugating enzyme 15

Gene

UBC15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei99 – 991Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. leaf morphogenesis Source: TAIR
  2. negative regulation of flower development Source: TAIR
  3. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G45050-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme 15 (EC:6.3.2.19)
Alternative name(s):
PM42
Ubiquitin carrier protein
Ubiquitin-conjugating enzyme E2-18 kDa 15
Ubiquitin-protein ligase
Gene namesi
Name:UBC15
Synonyms:UBC2-1
Ordered Locus Names:At1g45050
ORF Names:F27F5.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G45050.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161Ubiquitin-conjugating enzyme 15PRO_0000082575Add
BLAST

Proteomic databases

PaxDbiP42743.
PRIDEiP42743.

Expressioni

Gene expression databases

GenevestigatoriP42743.

Structurei

3D structure databases

ProteinModelPortaliP42743.
SMRiP42743. Positions 19-125.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233453.
InParanoidiP42743.
KOiK10688.
OMAiWIFTIEV.
PhylomeDBiP42743.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42743-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSSSAPSRK ALSKIACNRL QKELSEWQLN PPTGFRHKVT DNLQKWTIDV
60 70 80 90 100
TGAPGTLYAN ETYQLQVEFP EHYPMEAPQV VFVSPAPSHP HIYSNGHICL
110 120 130 140 150
DILYDSWSPA MTVNSVCISI LSMLSSSPAK QRPADNDRYV KNCKNGRSPK
160
ETRWWFHDDK V
Length:161
Mass (Da):18,262
Last modified:December 6, 2002 - v2
Checksum:i60C7F34A9574FB68
GO

Sequence cautioni

The sequence AAF69157.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331T → S in CAA48378. (PubMed:8219072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68306 mRNA. Translation: CAA48378.1.
AF028338 Genomic DNA. Translation: AAC39324.1.
DQ027029 mRNA. Translation: AAY44855.1.
AC007915 Genomic DNA. Translation: AAF69157.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32077.1.
BT026105 mRNA. Translation: ABG48461.1.
AK176757 mRNA. Translation: BAD44520.1.
PIRiC96509.
S39483.
RefSeqiNP_564493.1. NM_103582.3.
UniGeneiAt.204.

Genome annotation databases

EnsemblPlantsiAT1G45050.1; AT1G45050.1; AT1G45050.
GeneIDi841072.
KEGGiath:AT1G45050.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68306 mRNA. Translation: CAA48378.1 .
AF028338 Genomic DNA. Translation: AAC39324.1 .
DQ027029 mRNA. Translation: AAY44855.1 .
AC007915 Genomic DNA. Translation: AAF69157.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE32077.1 .
BT026105 mRNA. Translation: ABG48461.1 .
AK176757 mRNA. Translation: BAD44520.1 .
PIRi C96509.
S39483.
RefSeqi NP_564493.1. NM_103582.3.
UniGenei At.204.

3D structure databases

ProteinModelPortali P42743.
SMRi P42743. Positions 19-125.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P42743.
PRIDEi P42743.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G45050.1 ; AT1G45050.1 ; AT1G45050 .
GeneIDi 841072.
KEGGi ath:AT1G45050.

Organism-specific databases

TAIRi AT1G45050.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233453.
InParanoidi P42743.
KOi K10688.
OMAi WIFTIEV.
PhylomeDBi P42743.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT1G45050-MONOMER.

Gene expression databases

Genevestigatori P42743.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional expression and molecular characterization of AtUBC2-1, a novel ubiquitin-conjugating enzyme (E2) from Arabidopsis thaliana."
    Bartling D., Rehling P., Weiler E.W.
    Plant Mol. Biol. 23:387-396(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: cv. Landsberg erecta.
    Tissue: Leaf.
  2. "A new family of ubiquitin-conjugating enzymes (E2S) AtUBC15/16/17/18 in Arabidopsis thaliana."
    Yan N., Doelling J., Vierstra R.D.
    Plant Gene Register PGR97-174
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
  4. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBC15_ARATH
AccessioniPrimary (citable) accession number: P42743
Secondary accession number(s): O48952, Q67XR1, Q9MAK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 6, 2002
Last modified: October 1, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3