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Protein

Proteasome subunit beta type-1

Gene

PBF1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-ATH-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-ATH-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-ATH-5632684 Hedgehog 'on' state
R-ATH-5687128 MAPK6/MAPK4 signaling
R-ATH-5689603 UCH proteinases
R-ATH-5689880 Ub-specific processing proteases
R-ATH-6798695 Neutrophil degranulation
R-ATH-68949 Orc1 removal from chromatin
R-ATH-69017 CDK-mediated phosphorylation and removal of Cdc6
R-ATH-69229 Ubiquitin-dependent degradation of Cyclin D1
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-1 (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit F-1
Proteasome component 5
Proteasome subunit beta type-6
TAS-F22/FAFP98
TAS-G39.20
Gene namesi
Name:PBF1
Synonyms:PRC5
Ordered Locus Names:At3g60820
ORF Names:T4C21_230
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G60820
TAIRilocus:2101846 AT3G60820

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001480381 – 223Proteasome subunit beta type-1Add BLAST223

Proteomic databases

PaxDbiP42742
PRIDEiP42742

PTM databases

iPTMnetiP42742

Expressioni

Tissue specificityi

Present in all tissues examined. Slightly lower levels in roots.1 Publication

Developmental stagei

Expressed at maximal levels after first day of cell growth.

Inductioni

During cell proliferation.1 Publication

Gene expression databases

ExpressionAtlasiP42742 differential
GenevisibleiP42742 AT

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi10567, 14 interactors
IntActiP42742, 8 interactors
STRINGi3702.AT3G60820.1

Structurei

3D structure databases

ProteinModelPortaliP42742
SMRiP42742
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0179 Eukaryota
COG0638 LUCA
HOGENOMiHOG000091081
InParanoidiP42742
KOiK02732
OrthoDBiEOG09360LMI
PhylomeDBiP42742

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR016050 Proteasome_bsu_CS
IPR001353 Proteasome_sua/b
IPR023333 Proteasome_suB-type
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00854 PROTEASOME_BETA_1, 1 hit
PS51476 PROTEASOME_BETA_2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P42742-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTKQHANWSP YDNNGGTCVA IAGSDYCVIA ADTRMSTGYS ILSRDYSKIH
60 70 80 90 100
KLADRAVLSS SGFQADVKAL QKVLKSRHLI YQHQHNKQMS CPAMAQLLSN
110 120 130 140 150
TLYFKRFFPY YAFNVLGGLD EEGKGCVFTY DAVGSYERVG YGAQGSGSTL
160 170 180 190 200
IMPFLDNQLK SPSPLLLPKQ DSNTPLSEAE AVDLVKTVFA SATERDIYTG
210 220
DKLEIMILKA DGIKTELMDL RKD
Length:223
Mass (Da):24,644
Last modified:December 8, 2000 - v2
Checksum:iE8050079F353D4C0
GO

Sequence cautioni

The sequence CAA47753 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67338 mRNA Translation: CAA47753.1 Different initiation.
X79806 mRNA Translation: CAA56201.1
AF043537 mRNA Translation: AAC32073.1
AL162295 Genomic DNA Translation: CAB82686.1
CP002686 Genomic DNA Translation: AEE80112.1
CP002686 Genomic DNA Translation: AEE80114.1
AY062790 mRNA Translation: AAL32868.1
AY081571 mRNA Translation: AAM10133.1
AY086619 mRNA Translation: AAM63678.1
AK220787 mRNA Translation: BAD94023.1
Z26405 mRNA Translation: CAA81240.1
PIRiT47893
RefSeqiNP_001190146.1, NM_001203217.1 [P42742-1]
NP_191641.1, NM_115946.3 [P42742-1]
UniGeneiAt.20500
At.48789

Genome annotation databases

EnsemblPlantsiAT3G60820.1; AT3G60820.1; AT3G60820 [P42742-1]
AT3G60820.3; AT3G60820.3; AT3G60820 [P42742-1]
GeneIDi825253
GrameneiAT3G60820.1; AT3G60820.1; AT3G60820 [P42742-1]
AT3G60820.3; AT3G60820.3; AT3G60820 [P42742-1]
KEGGiath:AT3G60820

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSB1_ARATH
AccessioniPrimary (citable) accession number: P42742
Secondary accession number(s): P42741
, Q570C3, Q6LAD2, Q9SBI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 8, 2000
Last modified: May 23, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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