Reviewed, UniProtKB/Swiss-Prot P42737 (CAH2_ARATH)

Names and origin

Protein names

Recommended name:
    Carbonic anhydrase 2
    EC=4.2.1.1
Alternative name(s):
    Carbonate dehydratase 2

Gene names

Name:	CA2
Synonyms:	CA18
Ordered Locus Names:	At5g14740
ORF Names:	T9L3_40

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reversible hydration of carbon dioxide. This isoform ensures the supply of bicarbonate for pep carboxylase.
Catalytic activity	H2CO3 = CO2 + H2O.
Pathway	Photosynthesis; C4 acid pathway.
Subcellular location	Plastid › chloroplast membrane; Peripheral membrane protein. Plastid › chloroplast stroma. Ref.5
Induction	Expression reduced by 20% under dark conditions.
Sequence similarities	Belongs to the beta-class carbonic anhydrase family.
Sequence caution	The sequence CAC01873.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid
Coding sequence diversity	Alternative splicing
Ligand	Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbon utilization Inferred from electronic annotation. Source: InterPro defense response to bacterium Inferred from expression pattern. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast membrane Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast stroma Inferred from direct assay. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay. Source: TAIR cytosol Inferred from direct assay. Source: TAIR
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRX3	Q42403	1	EBI-449490,EBI-449157

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P42737-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Carbonic anhydrase 2		PRO_0000077458
Experimental info
	Sequence conflict	37 – 38	2	EL → DV in AAA50156. Ref.1
	Sequence conflict	215 – 216	2	LL → RC in AAA50156. Ref.1
	Sequence conflict	238	1	D → A in AAA50156. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 11, 2002. Version 2. Checksum: E329D4DAEF350CE0	FASTA	259	28,344
	10 20 30 40 50 60 MGNESYEDAI EALKKLLIEK DDLKDVAAAK VKKITAELQA ASSSDSKSFD PVERIKEGFV 70 80 90 100 110 120 TFKKEKYETN PALYGELAKG QSPKYMVFAC SDSRVCPSHV LDFHPGDAFV VRNIANMVPP 130 140 150 160 170 180 FDKVKYAGVG AAIEYAVLHL KVENIVVIGH SACGGIKGLM SFPLDGNNST DFIEDWVKIC 190 200 210 220 230 240 LPAKSKVLAE SESSAFEDQC GRCEREAVNV SLANLLTYPF VREGVVKGTL ALKGGYYDFV 250 NGSFELWELQ FGISPVHSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and expression of two cDNAs encoding carbonic anhydrase in Arabidopsis thaliana." Fett J.P., Coleman J.R. Plant Physiol. 105:707-713(1994) [PubMed: 7520589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs." Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H. Plant J. 9:101-124(1996) [PubMed: 8580968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 204-259. Strain: cv. Columbia. Tissue: Seedling.
[5]	"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana." Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N. Mol. Cell. Proteomics 2:325-345(2003) [PubMed: 12766230] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L18901 mRNA. Translation: AAA50156.1. AL391149 Genomic DNA. Translation: CAC01873.1. Sequence problems. AF324712 mRNA. Translation: AAG40063.1. AF326863 mRNA. Translation: AAG41445.1. AF339686 mRNA. Translation: AAK00368.1. AF428428 mRNA. Translation: AAL16197.1. BT000652 mRNA. Translation: AAN31799.1. BT000663 mRNA. Translation: AAN31810.1. Z26538 mRNA. Translation: CAA81309.1.
IPI	IPI00523587.
RefSeq	NP_974782.1.
UniGene	At.23743
3D structure databases
HSSP	HSSP built from PDB template 1EKJ based on UniProtKB P17067.
SMR	P42737. Positions 53-259.
ModBase	Search...
Protein-protein interaction databases
IntAct	P42737. 2 interactions.
2-D gel databases
SWISS-2DPAGE	P42737.
Proteomic databases
PRIDE	P42737.
ProMEX	P42737.
Genome annotation databases
GeneID	831326.
GenomeReviews	Gene locus AT5G14740 in contig BA000015_GR.
Organism-specific databases
TAIR	At5g14740.
Enzyme and pathway databases
BRENDA	4.2.1.1. 302.
Gene expression databases
ArrayExpress	P42737.
Family and domain databases
InterPro	IPR001765. Carbonic_anhydrase. IPR015892. Carbonic_anhydrase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1050.10. CO_anhd_prok_pln. 1 hit.
PANTHER	PTHR11002. Carbonic_anhydrase. 1 hit.
Pfam	PF00484. Pro_CA. 1 hit. [Graphical view]
PROSITE	PS00704. PROK_CO2_ANHYDRASE_1. 1 hit. PS00705. PROK_CO2_ANHYDRASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAH2_ARATH

Accession

Primary (citable) accession number: P42737
Secondary accession number(s): Q42116, Q9FDZ0, Q9LER2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 11, 2002
Last modified:	June 16, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)