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P42736 (RAP23_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ethylene-responsive transcription factor RAP2-3
Alternative name(s):
Cadmium-induced protein AS30
Ethylene response factor 72
Short name=ERF72
Ethylene-responsive element binding protein
Short name=AtEBP
Protein RELATED TO APETALA2 3
Short name=Related to AP2 3
Gene names
Name:RAP2-3
Synonyms:EBP, ERF072
Ordered Locus Names:At3g16770
ORF Names:MGL6.1, MGL6.24
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways By similarity. Ref.1

Subunit structure

Interacts with TGA4/OBF4, ACBP2, and ACBP4. Ref.1 Ref.8 Ref.10

Subcellular location

Nucleus. Cytoplasm. Cell membrane Ref.8 Ref.10.

Tissue specificity

Mostly expressed in stems and leaves, and, to a lower extent, in roots, siliques and flowers. Ref.3 Ref.10

Induction

By 1-aminocyclopropane-1-carboxylic acid (ACC, ethylene precursor), methyl jasmonate (MeJA), and Botrytis cinerea. Also induced by cadmium. Ref.1 Ref.10

Sequence similarities

Belongs to the AP2/ERF transcription factor family. ERF subfamily.

Contains 1 AP2/ERF DNA-binding domain.

Sequence caution

The sequence CAA71073.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

The sequence CAA85734.1 differs from that shown. Reason: Frameshift at position 20.

The sequence CAA85734.1 differs from that shown. Reason: Probable cloning artifact leading to an insertion into the sequence.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCell membrane
Cytoplasm
Membrane
Nucleus
   LigandCadmium
DNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell death

Inferred from mutant phenotype PubMed 15980186. Source: TAIR

ethylene-activated signaling pathway

Traceable author statement Ref.1. Source: TAIR

heat acclimation

Inferred from mutant phenotype PubMed 15980186. Source: TAIR

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 15980186. Source: TAIR

response to cytokinin

Inferred from expression pattern PubMed 16212609. Source: TAIR

response to ethylene

Inferred from expression pattern Ref.10. Source: UniProtKB

response to jasmonic acid

Inferred from expression pattern Ref.10. Source: UniProtKB

response to other organism

Inferred from expression pattern PubMed 10571872. Source: TAIR

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from direct assay Ref.10. Source: UniProtKB

nucleus

Inferred from direct assay Ref.8Ref.10. Source: UniProtKB

plasma membrane

Inferred from direct assay Ref.8. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from direct assay Ref.1. Source: UniProtKB

protein binding

Inferred from physical interaction Ref.8Ref.10Ref.1. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 15980186. Source: TAIR

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ACBP2Q9STP83EBI-368243,EBI-368234
ACBP4Q9MA554EBI-368243,EBI-2009699

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 248248Ethylene-responsive transcription factor RAP2-3
PRO_0000112523

Regions

DNA binding78 – 13558AP2/ERF
Compositional bias140 – 1434Poly-Pro

Experimental info

Sequence conflict421F → N in CAA85734. Ref.2
Sequence conflict1211A → P in CAA85734. Ref.2
Sequence conflict223 – 2242EQ → AR in CAA71073. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P42736 [UniParc].

Last modified January 10, 2003. Version 2.
Checksum: 317D50CC514782F1

FASTA24827,758
        10         20         30         40         50         60 
MCGGAIISDY APLVTKAKGR KLTAEELWSE LDASAADDFW GFYSTSKLHP TNQVNVKEEA 

        70         80         90        100        110        120 
VKKEQATEPG KRRKRKNVYR GIRKRPWGKW AAEIRDPRKG VRVWLGTFNT AEEAAMAYDV 

       130        140        150        160        170        180 
AAKQIRGDKA KLNFPDLHHP PPPNYTPPPS SPRSTDQPPA KKVCVVSQSE SELSQPSFPV 

       190        200        210        220        230        240 
ECIGFGNGDE FQNLSYGFEP DYDLKQQISS LESFLELDGN TAEQPSQLDE SVSEVDMWML 


DDVIASYE 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein."
Buettner M., Singh K.B.
Proc. Natl. Acad. Sci. U.S.A. 94:5961-5966(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH TGA4/OBF4, INDUCTION BY ETHYLENE.
Strain: cv. Columbia.
Tissue: Callus.
[2]Choi S., Baek E., Lee S.
Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[3]"The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis."
Okamuro J.K., Caster B., Villarroel R., Van Montagu M., Jofuku K.D.
Proc. Natl. Acad. Sci. U.S.A. 94:7076-7081(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[4]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"Arabidopsis Acyl-CoA-binding protein ACBP2 interacts with an ethylene-responsive element-binding protein, AtEBP, via its ankyrin repeats."
Li H.-Y., Chye M.-L.
Plant Mol. Biol. 54:233-243(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ACBP2.
[9]"Genome-wide analysis of the ERF gene family in Arabidopsis and rice."
Nakano T., Suzuki K., Fujimura T., Shinshi H.
Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[10]"Ethylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding protein."
Li H.-Y., Xiao S., Chye M.-L.
J. Exp. Bot. 59:3997-4006(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INDUCTION BY ETHYLENE; JASMONATE AND BOTRYTIS CINEREA, TISSUE SPECIFICITY, INTERACTION WITH ACBP4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09942 mRNA. Translation: CAA71073.1. Sequence problems.
Z37504 mRNA. Translation: CAA85734.1. Sequence problems.
AF003096 mRNA. Translation: AAC49769.1.
AB022217 Genomic DNA. Translation: BAB02769.1.
CP002686 Genomic DNA. Translation: AEE75863.1.
AY059917 mRNA. Translation: AAL24399.1.
AY035100 mRNA. Translation: AAK59605.1.
AY142562 mRNA. Translation: AAN13131.1.
AY087488 mRNA. Translation: AAM65031.1.
RefSeqNP_188299.1. NM_112550.4.
UniGeneAt.24773.

3D structure databases

ProteinModelPortalP42736.
SMRP42736. Positions 79-135.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid6263. 4 interactions.
IntActP42736. 3 interactions.
STRING3702.AT3G16770.1-P.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G16770.1; AT3G16770.1; AT3G16770.
GeneID820929.
KEGGath:AT3G16770.

Organism-specific databases

TAIRAT3G16770.

Phylogenomic databases

eggNOGNOG300006.
HOGENOMHOG000237624.
InParanoidP42736.
KOK09286.
OMAAEELWSE.
PhylomeDBP42736.

Gene expression databases

ArrayExpressP42736.
GenevestigatorP42736.

Family and domain databases

Gene3D3.30.730.10. 1 hit.
InterProIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamPF00847. AP2. 1 hit.
[Graphical view]
PRINTSPR00367. ETHRSPELEMNT.
SMARTSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMSSF54171. SSF54171. 1 hit.
PROSITEPS51032. AP2_ERF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRAP23_ARATH
AccessionPrimary (citable) accession number: P42736
Secondary accession number(s): O04382, O23105
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 10, 2003
Last modified: June 11, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names