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Protein

Ethylene-responsive transcription factor RAP2-3

Gene

RAP2-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi78 – 135AP2/ERFPROSITE-ProRule annotationAdd BLAST58

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cell death Source: TAIR
  • ethylene-activated signaling pathway Source: TAIR
  • heat acclimation Source: TAIR
  • positive regulation of transcription, DNA-templated Source: TAIR
  • response to cytokinin Source: TAIR
  • response to ethylene Source: UniProtKB
  • response to jasmonic acid Source: UniProtKB
  • response to other organism Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandCadmium

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene-responsive transcription factor RAP2-3
Alternative name(s):
Cadmium-induced protein AS30
Ethylene response factor 72
Short name:
ERF72
Ethylene-responsive element binding protein
Short name:
AtEBP
Protein RELATED TO APETALA2 3
Short name:
Related to AP2 3
Gene namesi
Name:RAP2-3
Synonyms:EBP, ERF072
Ordered Locus Names:At3g16770
ORF Names:MGL6.1, MGL6.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G16770.
TAIRilocus:2089368. AT3G16770.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi151S → A: Loss of phosphorylation by MAPK6. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001125231 – 248Ethylene-responsive transcription factor RAP2-3Add BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei151Phosphoserine; by MAPK61 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP42736.

Expressioni

Tissue specificityi

Mostly expressed in stems and leaves, and, to a lower extent, in roots, siliques and flowers.2 Publications

Inductioni

By 1-aminocyclopropane-1-carboxylic acid (ACC, ethylene precursor), methyl jasmonate (MeJA), and Botrytis cinerea. Also induced by cadmium.2 Publications

Gene expression databases

GenevisibleiP42736. AT.

Interactioni

Subunit structurei

Interacts with TGA4/OBF4, ACBP2, and ACBP4.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi6263. 7 interactors.
IntActiP42736. 3 interactors.
STRINGi3702.AT3G16770.1.

Structurei

3D structure databases

ProteinModelPortaliP42736.
SMRiP42736.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi140 – 143Poly-Pro4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IYVI. Eukaryota.
ENOG41113EY. LUCA.
HOGENOMiHOG000237624.
InParanoidiP42736.
KOiK09286.
OMAiDVWMIDD.
OrthoDBiEOG09360LUP.
PhylomeDBiP42736.

Family and domain databases

CDDicd00018. AP2. 1 hit.
Gene3Di3.30.730.10. 1 hit.
InterProiView protein in InterPro
IPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
PfamiView protein in Pfam
PF00847. AP2. 1 hit.
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiView protein in SMART
SM00380. AP2. 1 hit.
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiView protein in PROSITE
PS51032. AP2_ERF. 1 hit.

Sequencei

Sequence statusi: Complete.

P42736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGGAIISDY APLVTKAKGR KLTAEELWSE LDASAADDFW GFYSTSKLHP
60 70 80 90 100
TNQVNVKEEA VKKEQATEPG KRRKRKNVYR GIRKRPWGKW AAEIRDPRKG
110 120 130 140 150
VRVWLGTFNT AEEAAMAYDV AAKQIRGDKA KLNFPDLHHP PPPNYTPPPS
160 170 180 190 200
SPRSTDQPPA KKVCVVSQSE SELSQPSFPV ECIGFGNGDE FQNLSYGFEP
210 220 230 240
DYDLKQQISS LESFLELDGN TAEQPSQLDE SVSEVDMWML DDVIASYE
Length:248
Mass (Da):27,758
Last modified:January 10, 2003 - v2
Checksum:i317D50CC514782F1
GO

Sequence cautioni

The sequence CAA71073 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated
The sequence CAA85734 differs from that shown. Probable cloning artifact leading to an insertion into the sequence.Curated
The sequence CAA85734 differs from that shown. Reason: Frameshift at position 20.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42F → N in CAA85734 (Ref. 2) Curated1
Sequence conflicti121A → P in CAA85734 (Ref. 2) Curated1
Sequence conflicti223 – 224EQ → AR in CAA71073 (PubMed:9159183).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09942 mRNA. Translation: CAA71073.1. Sequence problems.
Z37504 mRNA. Translation: CAA85734.1. Sequence problems.
AF003096 mRNA. Translation: AAC49769.1.
AB022217 Genomic DNA. Translation: BAB02769.1.
CP002686 Genomic DNA. Translation: AEE75863.1.
AY059917 mRNA. Translation: AAL24399.1.
AY035100 mRNA. Translation: AAK59605.1.
AY142562 mRNA. Translation: AAN13131.1.
AY087488 mRNA. Translation: AAM65031.1.
RefSeqiNP_188299.1. NM_112550.5.
UniGeneiAt.24773.

Genome annotation databases

EnsemblPlantsiAT3G16770.1; AT3G16770.1; AT3G16770.
GeneIDi820929.
GrameneiAT3G16770.1; AT3G16770.1; AT3G16770.
KEGGiath:AT3G16770.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRAP23_ARATH
AccessioniPrimary (citable) accession number: P42736
Secondary accession number(s): O04382, O23105
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 10, 2003
Last modified: June 7, 2017
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families