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Protein

Probable cinnamyl alcohol dehydrogenase 9

Gene

CAD9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in lignin biosynthesis. May catalyze the final step specific for the production of lignin monomers, like coniferyl alcohol, sinapyl alcohol and 4-coumaryl alcohol.1 Publication

Catalytic activityi

Cinnamyl alcohol + NADP+ = cinnamaldehyde + NADPH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Pathwayi: phenylpropanoid biosynthesis

This protein is involved in the pathway phenylpropanoid biosynthesis, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway phenylpropanoid biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50Zinc 1; catalyticBy similarity1
Binding sitei52NADPBy similarity1
Metal bindingi72Zinc 1; catalyticBy similarity1
Metal bindingi73Zinc 1; catalyticBy similarity1
Metal bindingi103Zinc 2By similarity1
Metal bindingi106Zinc 2By similarity1
Metal bindingi109Zinc 2By similarity1
Metal bindingi117Zinc 2By similarity1
Metal bindingi166Zinc 1; catalyticBy similarity1
Binding sitei170NADPBy similarity1
Binding sitei254NADPBy similarity1
Binding sitei278NADP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi191 – 196NADPBy similarity6
Nucleotide bindingi214 – 219NADPBy similarity6
Nucleotide bindingi301 – 303NADPBy similarity3

GO - Molecular functioni

  • cinnamyl-alcohol dehydrogenase activity Source: UniProtKB
  • sinapyl alcohol dehydrogenase activity Source: UniProtKB-EC
  • zinc ion binding Source: InterPro

GO - Biological processi

  • lignin biosynthetic process Source: UniProtKB
  • response to cytokinin Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processLignin biosynthesis
LigandMetal-binding, NADP, Zinc

Enzyme and pathway databases

BioCyciARA:AT4G39330-MONOMER
UniPathwayiUPA00711

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cinnamyl alcohol dehydrogenase 9 (EC:1.1.1.195)
Short name:
AtCAD9
Gene namesi
Name:CAD9
Synonyms:CAD1
Ordered Locus Names:At4g39330
ORF Names:T22F8.230
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G39330
TAIRilocus:2136278 AT4G39330

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001608101 – 360Probable cinnamyl alcohol dehydrogenase 9Add BLAST360

Proteomic databases

PaxDbiP42734
PRIDEiP42734

PTM databases

iPTMnetiP42734

Expressioni

Tissue specificityi

Expressed in the vasculature of the primary root and elongation regions. Expressed in the hypocotyl, cotyledon veins, vasculature of the first rosette leaves, and hydathodes. In stems, expressed in the vascular cambium, interfascicular cambium, developing xylem, and phloem. Expressed in the entire floral organs at late developing stage, and in the abscission, style and stigmatic regions of siliques and seed funicules.2 Publications

Gene expression databases

ExpressionAtlasiP42734 baseline and differential
GenevisibleiP42734 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi15368, 1 interactor
IntActiP42734, 1 interactor
STRINGi3702.AT4G39330.1

Structurei

3D structure databases

ProteinModelPortaliP42734
SMRiP42734
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0023 Eukaryota
COG1064 LUCA
HOGENOMiHOG000294667
InParanoidiP42734
KOiK00083
OMAiWRGPAHF
OrthoDBiEOG09360F7A
PhylomeDBiP42734

Family and domain databases

InterProiView protein in InterPro
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P42734-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKSPETEHP NKVFGWGARD KSGVLSPFHF SRRDNGENDV TVKILFCGVC
60 70 80 90 100
HTDLHTIKND WGYSYYPVVP GHEIVGIATK VGKNVTKFKE GDRVGVGVIS
110 120 130 140 150
GSCQSCESCD QDLENYCPQM SFTYNAIGSD GTKNYGGYSE NIVVDQRFVL
160 170 180 190 200
RFPENLPSDS GAPLLCAGIT VYSPMKYYGM TEAGKHLGVA GLGGLGHVAV
210 220 230 240 250
KIGKAFGLKV TVISSSSTKA EEAINHLGAD SFLVTTDPQK MKAAIGTMDY
260 270 280 290 300
IIDTISAVHA LYPLLGLLKV NGKLIALGLP EKPLELPMFP LVLGRKMVGG
310 320 330 340 350
SDVGGMKETQ EMLDFCAKHN ITADIELIKM DEINTAMERL AKSDVRYRFV
360
IDVANSLSPP
Length:360
Mass (Da):38,934
Last modified:May 29, 2007 - v2
Checksum:i0E4F3B0581785759
GO
Isoform 2 (identifier: P42734-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     302-311: DVGGMKETQE → LLSLMLGTGS
     312-360: Missing.

Note: No experimental confirmation available.
Show »
Length:311
Mass (Da):33,253
Checksum:i539EE3E5D40C94CE
GO

Sequence cautioni

The sequence BAH56862 differs from that shown. Reason: Frameshift at position 300.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti202I → S in AAA99511 (PubMed:7630954).Curated1
Sequence conflicti352D → N in AAM64913 (PubMed:19423640).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037894302 – 311DVGGMKETQE → LLSLMLGTGS in isoform 2. 1 Publication10
Alternative sequenceiVSP_037895312 – 360Missing in isoform 2. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37883 Genomic DNA Translation: AAA99511.1
L37884 mRNA Translation: AAA74746.1
AY302076 mRNA Translation: AAP59429.1
AL050351 Genomic DNA Translation: CAB43648.1
AL161595 Genomic DNA Translation: CAB80596.1
CP002687 Genomic DNA Translation: AEE87056.1
CP002687 Genomic DNA Translation: AEE87057.1
AF370498 mRNA Translation: AAK43875.1
AY064669 mRNA Translation: AAL47376.1
AK317632 mRNA Translation: BAH20294.1
AK318747 mRNA Translation: BAH56862.1 Frameshift.
AY087363 mRNA Translation: AAM64913.1
PIRiS71179
T08581
RefSeqiNP_001031812.1, NM_001036735.2 [P42734-2]
NP_195643.1, NM_120093.5 [P42734-1]
UniGeneiAt.24772
At.67820
At.75608

Genome annotation databases

EnsemblPlantsiAT4G39330.1; AT4G39330.1; AT4G39330 [P42734-1]
AT4G39330.2; AT4G39330.2; AT4G39330 [P42734-2]
GeneIDi830088
GrameneiAT4G39330.1; AT4G39330.1; AT4G39330 [P42734-1]
AT4G39330.2; AT4G39330.2; AT4G39330 [P42734-2]
KEGGiath:AT4G39330

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCADH9_ARATH
AccessioniPrimary (citable) accession number: P42734
Secondary accession number(s): B9DHS7
, C0Z2D3, Q8LB84, Q94K02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 29, 2007
Last modified: April 25, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health