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Protein

Chaperone protein ClpB1

Gene

CLPB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular chaperone that plays an important role in thermotolerance. Together with HSA32, required for long-term acquired thermotolerance (LAT) in plants and naturally high basal thermotolerance observed in germinating seedlings.7 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi207 – 214ATPSequence analysis8
Nucleotide bindingi606 – 613ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • positive regulation of translation Source: TAIR
  • protein metabolic process Source: InterPro
  • protein unfolding Source: TAIR
  • response to heat Source: TAIR
  • response to high light intensity Source: TAIR
  • response to hydrogen peroxide Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB1
Alternative name(s):
ATP-dependent Clp protease ATP-binding subunit ClpB homolog 1
Casein lytic proteinase B1
Heat shock protein 101
Protein DEFECTIVE IN LONG-TERM ACQUIRED THERMOTOLERANCE1 Publication
Gene namesi
Name:CLPB1
Synonyms:DLT11 Publication, HOT1, HSP101
Ordered Locus Names:At1g74310
ORF Names:F1O17.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G74310.

Subcellular locationi

GO - Cellular componenti

  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytosol Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but germinating seeds have greatly reduced basal thermotolerance and are unable to acquire thermotolerance (PubMed:10760305, PubMed:11489180). Faster degradation of HSA32 (at protein level) (PubMed:23439916).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33H → Y in dlt1-2; normal growth and development under nonstress conditions. Impaired chaperone activity and thermotolerance function, but normal positive regulation of HSA32 during recovery after heat treatment. 1 Publication1
Mutagenesisi499A → T in hot1-4; reduced basal thermotolerance and unable to acquire thermotolerance. 1 Publication1
Mutagenesisi509E → K in hot1-6; reduced ability to acquire thermotolerance. 1 Publication1
Mutagenesisi599T → I in dlt1-1; normal growth and development under nonstress conditions, and normal chaperone activity and thermotolerance function. Compromised positive regulation of HSA32 during recovery after heat treatment. 1 Publication1
Mutagenesisi637E → K in hot1-1; greatly reduced basal thermotolerance and unable to acquire thermotolerance. 2 Publications1
Mutagenesisi706R → K in hot1-5; unable to acquire thermotolerance. 1 Publication1
Mutagenesisi815G → D in hot1-7; reduced ability to acquire thermotolerance. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001912201 – 911Chaperone protein ClpB1Add BLAST911

Proteomic databases

PaxDbiP42730.
PRIDEiP42730.

Expressioni

Developmental stagei

Starts to accumulate in maturing seeds 15 days after pollination and decreases rapidly during seed germination.2 Publications

Inductioni

By heat stress and oilseed rape mosaic virus (PubMed:10760238, PubMed:10760305, PubMed:11489180, PubMed:16644052, PubMed:16995899, PubMed:17144892, PubMed:7866032). Regulated by HSA32 that retards its decay in a positive feedback loop (at protein level) during recovery after heat treatment (PubMed:23439916).8 Publications

Gene expression databases

GenevisibleiP42730. AT.

Interactioni

Protein-protein interaction databases

BioGridi28990. 2 interactors.
IntActiP42730. 1 interactor.
STRINGi3702.AT1G74310.1.

Structurei

3D structure databases

ProteinModelPortaliP42730.
SMRiP42730.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 410IAdd BLAST247
Regioni532 – 723IIAdd BLAST192

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili403 – 500Sequence analysisAdd BLAST98

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1051. Eukaryota.
COG0542. LUCA.
HOGENOMiHOG000218211.
InParanoidiP42730.
KOiK03695.
OMAiHKTNETI.
OrthoDBiEOG093602DK.
PhylomeDBiP42730.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42730-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPEKFTHKT NETIATAHEL AVNAGHAQFT PLHLAGALIS DPTGIFPQAI
60 70 80 90 100
SSAGGENAAQ SAERVINQAL KKLPSQSPPP DDIPASSSLI KVIRRAQAAQ
110 120 130 140 150
KSRGDTHLAV DQLIMGLLED SQIRDLLNEV GVATARVKSE VEKLRGKEGK
160 170 180 190 200
KVESASGDTN FQALKTYGRD LVEQAGKLDP VIGRDEEIRR VVRILSRRTK
210 220 230 240 250
NNPVLIGEPG VGKTAVVEGL AQRIVKGDVP NSLTDVRLIS LDMGALVAGA
260 270 280 290 300
KYRGEFEERL KSVLKEVEDA EGKVILFIDE IHLVLGAGKT EGSMDAANLF
310 320 330 340 350
KPMLARGQLR CIGATTLEEY RKYVEKDAAF ERRFQQVYVA EPSVPDTISI
360 370 380 390 400
LRGLKEKYEG HHGVRIQDRA LINAAQLSAR YITGRHLPDK AIDLVDEACA
410 420 430 440 450
NVRVQLDSQP EEIDNLERKR MQLEIELHAL EREKDKASKA RLIEVRKELD
460 470 480 490 500
DLRDKLQPLT MKYRKEKERI DEIRRLKQKR EELMFSLQEA ERRYDLARAA
510 520 530 540 550
DLRYGAIQEV ESAIAQLEGT SSEENVMLTE NVGPEHIAEV VSRWTGIPVT
560 570 580 590 600
RLGQNEKERL IGLADRLHKR VVGQNQAVNA VSEAILRSRA GLGRPQQPTG
610 620 630 640 650
SFLFLGPTGV GKTELAKALA EQLFDDENLL VRIDMSEYME QHSVSRLIGA
660 670 680 690 700
PPGYVGHEEG GQLTEAVRRR PYCVILFDEV EKAHVAVFNT LLQVLDDGRL
710 720 730 740 750
TDGQGRTVDF RNSVIIMTSN LGAEHLLAGL TGKVTMEVAR DCVMREVRKH
760 770 780 790 800
FRPELLNRLD EIVVFDPLSH DQLRKVARLQ MKDVAVRLAE RGVALAVTDA
810 820 830 840 850
ALDYILAESY DPVYGARPIR RWMEKKVVTE LSKMVVREEI DENSTVYIDA
860 870 880 890 900
GAGDLVYRVE SGGLVDASTG KKSDVLIHIA NGPKRSDAAQ AVKKMRIEEI
910
EDDDNEEMIE D
Length:911
Mass (Da):101,295
Last modified:May 27, 2002 - v2
Checksum:i191EC1853B0C4CB9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti141V → F in AAL32674 (PubMed:14593172).Curated1
Sequence conflicti595P → A in AAA67927 (PubMed:7866032).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13949 mRNA. Translation: AAA67927.1.
AF218796 Genomic DNA. Translation: AAF26423.1.
AC020579 Genomic DNA. Translation: AAG52410.1.
CP002684 Genomic DNA. Translation: AEE35576.1.
AY062596 mRNA. Translation: AAL32674.1.
PIRiF96771.
RefSeqiNP_565083.1. NM_106091.4.
UniGeneiAt.48370.
At.69978.

Genome annotation databases

EnsemblPlantsiAT1G74310.1; AT1G74310.1; AT1G74310.
GeneIDi843771.
GrameneiAT1G74310.1; AT1G74310.1; AT1G74310.
KEGGiath:AT1G74310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13949 mRNA. Translation: AAA67927.1.
AF218796 Genomic DNA. Translation: AAF26423.1.
AC020579 Genomic DNA. Translation: AAG52410.1.
CP002684 Genomic DNA. Translation: AEE35576.1.
AY062596 mRNA. Translation: AAL32674.1.
PIRiF96771.
RefSeqiNP_565083.1. NM_106091.4.
UniGeneiAt.48370.
At.69978.

3D structure databases

ProteinModelPortaliP42730.
SMRiP42730.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28990. 2 interactors.
IntActiP42730. 1 interactor.
STRINGi3702.AT1G74310.1.

Proteomic databases

PaxDbiP42730.
PRIDEiP42730.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G74310.1; AT1G74310.1; AT1G74310.
GeneIDi843771.
GrameneiAT1G74310.1; AT1G74310.1; AT1G74310.
KEGGiath:AT1G74310.

Organism-specific databases

TAIRiAT1G74310.

Phylogenomic databases

eggNOGiKOG1051. Eukaryota.
COG0542. LUCA.
HOGENOMiHOG000218211.
InParanoidiP42730.
KOiK03695.
OMAiHKTNETI.
OrthoDBiEOG093602DK.
PhylomeDBiP42730.

Miscellaneous databases

PROiP42730.

Gene expression databases

GenevisibleiP42730. AT.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPB1_ARATH
AccessioniPrimary (citable) accession number: P42730
Secondary accession number(s): Q8W4F2, Q9LE57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 27, 2002
Last modified: November 30, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Over-expression of HSP101 partially reverse the sensitivity of 14 day-old seedling to heat stress.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.