P42719 (PYRE_RHILT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:

pyrE

Organism

Rhizobium leguminosarum bv. trifolii

Taxonomic identifier

386 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. Ref.1
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.
Biophysicochemical properties	Kinetic parameters: KM=27.6 µM for orotate Ref.1 Vmax=120 µmol/min/mg enzyme pH dependence: Optimum pH is 10.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110728
Regions
	Region	133 – 141	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	107	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	108	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	111	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	113	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	137	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42719 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 9C758A9C9E8D27D2	FASTA	223	24,623
	10 20 30 40 50 60 MIQTTFPDRA VMAELLAKML WEIKAVHFNA AQPYKLASGM ASPVYIDCRK LLSFPRIRST 70 80 90 100 110 120 VMDFAASTLL RDAGFEQFDC IAGGETAGIP FAALLADRLG LPMIYVRKQP KGHGRNAQIE 130 140 150 160 170 180 GNMPEGSRVL VIEDLTTAGG SMFKFIDAVR AAGGIVDHGI ALFFYGIFGE QRFADGKVRL 190 200 210 220 HHIATWRNVL PSPGSRSSST TRRCRKSSPS SMRRWLGRER MVA

References

[1]	"Sequence and phylogenetic analysis of the Rhizobium leguminosarum biovar trifolii pyrE gene, overproduction, purification and characterization of orotate phosphoribosyltransferase." Bayles D.O., Fennington G.J. Jr., Hughes T.A. Gene 195:329-336(1997) [PubMed: 9305779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U08434 Unassigned DNA. Translation: AAB52372.1.
3D structure databases
ProteinModelPortal	P42719.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_RHILT

Accession

Primary (citable) accession number: P42719

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 21, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program