P42709 (VDH_STRAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Valine dehydrogenase
Short name=ValDH
EC=1.4.1.-

Gene names

Name:

vdh

Organism

Streptomyces ambofaciens

Taxonomic identifier

1889 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	106 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxidative deamination of branched-chain amino acids. The catabolism of valine is the major source of fatty acid precursors for macrolide biosynthesis and a vital source of antibiotic precursors.
Catalytic activity	L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH.
Pathway	Amino-acid degradation; L-valine degradation.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Biological process	Branched-chain amino acid catabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	valine catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – ›106	›105	Valine dehydrogenase		PRO_0000182811
Sites
	Active site	91	1	By similarity
Experimental info
	Non-terminal residue	106	1

Sequences

	Sequence		Length	Mass (Da)
	P42709 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 1A1116020A884ADE	FASTA	106	10,964
	10 20 30 40 50 60 MTDVTGAPAD VLHTLFHSDQ GGHEQVVLCQ DRASGLKAVI ALHSTALGPA LGGTRFYPIA 70 80 90 100 NEAEAVADAL NLARGMSYKN AMAGLEHGGG KAVIIGDPEQ IKSEEL

References

[1]

"Amino acid catabolism and antibiotic synthesis: valine is a source of precursors for macrolide biosynthesis in Streptomyces ambofaciens and Streptomyces fradiae." Tang L., Zhang Y.X., Hutchinson C.R. J. Bacteriol. 176:6107-6119(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15154 / NBRC 13685 / NRRL 2420 / Isolate B3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L33871 Genomic DNA. Translation: AAA62597.1.
3D structure databases
ProteinModelPortal	P42709.
SMR	P42709. Positions 23-106.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00362.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR016211. Glu/Phe/Leu/Val_DH_bac/arc. [Graphical view]
PANTHER	PTHR11606:SF3. PTHR11606:SF3. 1 hit.
Pfam	PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VDH_STRAM

Accession

Primary (citable) accession number: P42709

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program