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P42703

- LIFR_MOUSE

UniProt

P42703 - LIFR_MOUSE

Protein

Leukemia inhibitory factor receptor

Gene

Lifr

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.

    GO - Molecular functioni

    1. leukemia inhibitory factor receptor activity Source: Ensembl
    2. oncostatin-M receptor activity Source: Ensembl
    3. protein binding Source: MGI

    GO - Biological processi

    1. positive regulation of cell proliferation Source: MGI

    Keywords - Molecular functioni

    Receptor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leukemia inhibitory factor receptor
    Short name:
    LIF receptor
    Short name:
    LIF-R
    Alternative name(s):
    D-factor/LIF receptor
    CD_antigen: CD118
    Gene namesi
    Name:Lifr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:96788. Lifr.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4343Sequence AnalysisAdd
    BLAST
    Chaini44 – 10921049Leukemia inhibitory factor receptorPRO_0000010903Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi53 ↔ 631 Publication
    Disulfide bondi80 ↔ 881 Publication
    Glycosylationi164 – 1641N-linked (GlcNAc...)1 Publication
    Glycosylationi199 – 1991N-linked (GlcNAc...)1 Publication
    Disulfide bondi208 ↔ 2651 Publication
    Glycosylationi238 – 2381N-linked (GlcNAc...)1 Publication
    Glycosylationi261 – 2611N-linked (GlcNAc...)1 Publication
    Disulfide bondi336 ↔ 3461 Publication
    Glycosylationi385 – 3851N-linked (GlcNAc...)4 Publications
    Glycosylationi402 – 4021N-linked (GlcNAc...)2 Publications
    Glycosylationi421 – 4211N-linked (GlcNAc...)1 Publication
    Glycosylationi440 – 4401N-linked (GlcNAc...)1 Publication
    Glycosylationi453 – 4531N-linked (GlcNAc...)1 Publication
    Disulfide bondi461 ↔ 5061 Publication
    Glycosylationi476 – 4761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi567 – 5671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi647 – 6471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi658 – 6581N-linked (GlcNAc...)1 Publication
    Glycosylationi675 – 6751N-linked (GlcNAc...)1 Publication
    Glycosylationi724 – 7241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi782 – 7821N-linked (GlcNAc...)Sequence Analysis
    Modified residuei922 – 9221PhosphoserineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP42703.
    PaxDbiP42703.
    PRIDEiP42703.

    PTM databases

    PhosphoSiteiP42703.

    Expressioni

    Tissue specificityi

    Placenta, liver, kidney, heart, lung, brain, and embryos. The liver may be the primary site of synthesis of the secreted form.

    Gene expression databases

    BgeeiP42703.
    CleanExiMM_LIFR.
    GenevestigatoriP42703.

    Interactioni

    Subunit structurei

    Heterodimer composed of LIFR and IL6ST. The heterodimer formed by LIFR and IL6ST interacts with the complex formed by CNTF and CNTFR By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201163. 3 interactions.
    DIPiDIP-5772N.
    IntActiP42703. 3 interactions.
    MINTiMINT-4100386.

    Structurei

    Secondary structure

    1
    1092
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi54 – 6411
    Beta strandi69 – 713
    Beta strandi76 – 9015
    Beta strandi92 – 998
    Beta strandi106 – 11510
    Beta strandi117 – 1226
    Beta strandi136 – 1383
    Turni143 – 1464
    Beta strandi147 – 1526
    Helixi156 – 1583
    Beta strandi161 – 17010
    Beta strandi174 – 1774
    Beta strandi190 – 1923
    Beta strandi197 – 2015
    Beta strandi207 – 2093
    Beta strandi212 – 2198
    Beta strandi255 – 2595
    Beta strandi263 – 2664
    Beta strandi272 – 2776
    Beta strandi288 – 2903
    Beta strandi292 – 2954
    Beta strandi307 – 3159
    Beta strandi317 – 3259
    Beta strandi335 – 34814
    Beta strandi363 – 3686
    Turni369 – 3713
    Beta strandi374 – 3785
    Beta strandi388 – 3936
    Beta strandi402 – 4098
    Beta strandi412 – 4198
    Helixi422 – 4243
    Beta strandi432 – 4387
    Beta strandi444 – 45310
    Beta strandi455 – 46612
    Beta strandi473 – 4808
    Beta strandi483 – 4919
    Beta strandi504 – 5107

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2Q7NX-ray4.00A/C50-529[»]
    ProteinModelPortaliP42703.
    SMRiP42703. Positions 50-817.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP42703.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini44 – 828785ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini854 – 1092239CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei829 – 85325HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 12682Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini330 – 429100Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini430 – 529100Fibronectin type-III 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini533 – 62492Fibronectin type-III 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini622 – 71493Fibronectin type-III 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini719 – 828110Fibronectin type-III 6PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi514 – 5185WSXWS motif
    Motifi864 – 8729Box 1 motif

    Domaini

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

    Sequence similaritiesi

    Contains 6 fibronectin type-III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG147644.
    GeneTreeiENSGT00550000074436.
    HOGENOMiHOG000113324.
    HOVERGENiHBG006266.
    InParanoidiQ5I0Y2.
    KOiK05058.
    OMAiFYPDIPN.
    OrthoDBiEOG7V49XP.
    PhylomeDBiP42703.
    TreeFamiTF338122.

    Family and domain databases

    Gene3Di2.60.40.10. 4 hits.
    InterProiIPR003961. Fibronectin_type3.
    IPR003529. Hematopoietin_rcpt_Gp130_CS.
    IPR013783. Ig-like_fold.
    [Graphical view]
    PfamiPF00041. fn3. 1 hit.
    [Graphical view]
    SMARTiSM00060. FN3. 5 hits.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 3 hits.
    PROSITEiPS50853. FN3. 4 hits.
    PS01353. HEMATOPO_REC_L_F2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P42703-1) [UniParc]FASTAAdd to Basket

    Also known as: Membrane

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAYSWWRQP SWMVDNKRSR MTPNLPWLLS ALTLLHLTMH ANGLKRGVQD     50
    LKCTTNNMRV WDCTWPAPLG VSPGTVKDIC IKDRFHSCHP LETTNVKIPA 100
    LSPGDHEVTI NYLNGFQSKF TLNEKDVSLI PETPEILDLS ADFFTSSLLL 150
    KWNDRGSALP HPSNATWEIK VLQNPRTEPV ALVLLNTMLS GKDTVQHWNW 200
    TSDLPLQCAT HSVSIRWHID SPHFSGYKEW SDWSPLKNIS WIRNTETNVF 250
    PQDKVVLAGS NMTICCMSPT KVLSGQIGNT LRPLIHLYGQ TVAIHILNIP 300
    VSENSGTNII FITDDDVYGT VVFAGYPPDV PQKLSCETHD LKEIICSWNP 350
    GRITGLVGPR NTEYTLFESI SGKSAVFHRI EGLTNETYRL GVQMHPGQEI 400
    HNFTLTGRNP LGQAQSAVVI NVTERVAPHD PTSLKVKDIN STVVTFSWYL 450
    PGNFTKINLL CQIEICKANS KKEVRNATIR GAEDSTYHVA VDKLNPYTAY 500
    TFRVRCSSKT FWKWSRWSDE KRHLTTEATP SKGPDTWREW SSDGKNLIVY 550
    WKPLPINEAN GKILSYNVSC SLNEETQSVL EIFDPQHRAE IQLSKNDYII 600
    SVVARNSAGS SPPSKIASME IPNDDITVEQ AVGLGNRIFL TWRHDPNMTC 650
    DYVIKWCNSS RSEPCLLDWR KVPSNSTETV IESDQFQPGV RYNFYLYGCT 700
    NQGYQLLRSI IGYVEELAPI VAPNFTVEDT SADSILVKWD DIPVEELRGF 750
    LRGYLFYFQK GERDTPKTRS LEPHHSDIKL KNITDISQKT LRIADLQGKT 800
    SYHLVLRAYT HGGLGPEKSM FVVTKENSVG LIIAILIPVA VAVIVGVVTS 850
    ILCYRKREWI KETFYPDIPN PENCKALQFQ KSVCEGSNAL KTLEMNPCTP 900
    NNVEVLESRS IVPKIEDTEI ISPVAERPGE RSEVDPENHV VVSYCPPIIE 950
    EEITNPAADE VGGASQVVYI DVQSMYQPQA KAEEEQDVDP VVVAGYKPQM 1000
    RLPISPAVED TAAEDEEGKT AGYRPQANVN TWNLVSPDSP RSTDSNNEVV 1050
    SFGSPCSINS RQFLIPPKDE DSPKSNGGGW SFTNFFQNKP ND 1092
    Length:1,092
    Mass (Da):122,574
    Last modified:November 1, 1995 - v1
    Checksum:i6F02BBC8E154DE70
    GO
    Isoform 2 (identifier: P42703-2) [UniParc]FASTAAdd to Basket

    Also known as: Secreted

    The sequence of this isoform differs from the canonical sequence as follows:
         718-719: AP → EA
         720-1092: Missing.

    Show »
    Length:719
    Mass (Da):81,287
    Checksum:i4DF1779816B06D2B
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei718 – 7192AP → EA in isoform 2. 4 PublicationsVSP_001686
    Alternative sequencei720 – 1092373Missing in isoform 2. 4 PublicationsVSP_001687Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S73496 mRNA. Translation: AAC60698.1.
    S73495 mRNA. Translation: AAC60697.1.
    D26177 mRNA. Translation: BAA05165.1.
    D17444 mRNA. Translation: BAA04258.1.
    BC031929 mRNA. Translation: AAH31929.1.
    CCDSiCCDS27369.1. [P42703-1]
    CCDS49577.1. [P42703-2]
    PIRiJX0312.
    RefSeqiNP_001106857.1. NM_001113386.1. [P42703-2]
    NP_038612.1. NM_013584.2. [P42703-1]
    XP_006520030.1. XM_006519967.1. [P42703-1]
    XP_006520031.1. XM_006519968.1. [P42703-1]
    XP_006520032.1. XM_006519969.1. [P42703-1]
    XP_006520033.1. XM_006519970.1. [P42703-2]
    XP_006520034.1. XM_006519971.1. [P42703-2]
    UniGeneiMm.149720.

    Genome annotation databases

    EnsembliENSMUST00000067190; ENSMUSP00000064551; ENSMUSG00000054263. [P42703-1]
    ENSMUST00000164529; ENSMUSP00000131434; ENSMUSG00000054263. [P42703-2]
    ENSMUST00000171588; ENSMUSP00000126137; ENSMUSG00000054263. [P42703-1]
    GeneIDi16880.
    KEGGimmu:16880.
    UCSCiuc007vdw.2. mouse. [P42703-2]
    uc007vdx.2. mouse. [P42703-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S73496 mRNA. Translation: AAC60698.1 .
    S73495 mRNA. Translation: AAC60697.1 .
    D26177 mRNA. Translation: BAA05165.1 .
    D17444 mRNA. Translation: BAA04258.1 .
    BC031929 mRNA. Translation: AAH31929.1 .
    CCDSi CCDS27369.1. [P42703-1 ]
    CCDS49577.1. [P42703-2 ]
    PIRi JX0312.
    RefSeqi NP_001106857.1. NM_001113386.1. [P42703-2 ]
    NP_038612.1. NM_013584.2. [P42703-1 ]
    XP_006520030.1. XM_006519967.1. [P42703-1 ]
    XP_006520031.1. XM_006519968.1. [P42703-1 ]
    XP_006520032.1. XM_006519969.1. [P42703-1 ]
    XP_006520033.1. XM_006519970.1. [P42703-2 ]
    XP_006520034.1. XM_006519971.1. [P42703-2 ]
    UniGenei Mm.149720.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2Q7N X-ray 4.00 A/C 50-529 [» ]
    ProteinModelPortali P42703.
    SMRi P42703. Positions 50-817.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201163. 3 interactions.
    DIPi DIP-5772N.
    IntActi P42703. 3 interactions.
    MINTi MINT-4100386.

    PTM databases

    PhosphoSitei P42703.

    Proteomic databases

    MaxQBi P42703.
    PaxDbi P42703.
    PRIDEi P42703.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000067190 ; ENSMUSP00000064551 ; ENSMUSG00000054263 . [P42703-1 ]
    ENSMUST00000164529 ; ENSMUSP00000131434 ; ENSMUSG00000054263 . [P42703-2 ]
    ENSMUST00000171588 ; ENSMUSP00000126137 ; ENSMUSG00000054263 . [P42703-1 ]
    GeneIDi 16880.
    KEGGi mmu:16880.
    UCSCi uc007vdw.2. mouse. [P42703-2 ]
    uc007vdx.2. mouse. [P42703-1 ]

    Organism-specific databases

    CTDi 3977.
    MGIi MGI:96788. Lifr.

    Phylogenomic databases

    eggNOGi NOG147644.
    GeneTreei ENSGT00550000074436.
    HOGENOMi HOG000113324.
    HOVERGENi HBG006266.
    InParanoidi Q5I0Y2.
    KOi K05058.
    OMAi FYPDIPN.
    OrthoDBi EOG7V49XP.
    PhylomeDBi P42703.
    TreeFami TF338122.

    Miscellaneous databases

    EvolutionaryTracei P42703.
    NextBioi 290878.
    PROi P42703.
    SOURCEi Search...

    Gene expression databases

    Bgeei P42703.
    CleanExi MM_LIFR.
    Genevestigatori P42703.

    Family and domain databases

    Gene3Di 2.60.40.10. 4 hits.
    InterProi IPR003961. Fibronectin_type3.
    IPR003529. Hematopoietin_rcpt_Gp130_CS.
    IPR013783. Ig-like_fold.
    [Graphical view ]
    Pfami PF00041. fn3. 1 hit.
    [Graphical view ]
    SMARTi SM00060. FN3. 5 hits.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 3 hits.
    PROSITEi PS50853. FN3. 4 hits.
    PS01353. HEMATOPO_REC_L_F2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Leukemia inhibitory factor receptor is structurally related to the IL-6 signal transducer, gp130."
      Gearing D.P., Thut C.J., Vanden Bos T., Gimpel S.D., Delaney P.B., King J., Price V., Cosman D., Beckmann M.P.
      EMBO J. 10:2839-2848(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    2. "Pregnancy associated increase in mRNA for soluble D-factor/LIF receptor in mouse liver."
      Tomida M., Yamamoto-Yamaguchi Y., Hozumi M.
      FEBS Lett. 334:193-197(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: ICR.
      Tissue: Liver.
    3. "Three different cDNAs encoding mouse D-factor/LIF receptor."
      Tomida M., Yamamoto-Yamaguchi Y., Hozumi M.
      J. Biochem. 115:557-562(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: FVB/N.
      Tissue: Liver.
    5. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
      Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
      J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-385; ASN-402 AND ASN-675.
      Strain: C57BL/6.
      Tissue: Plasma.
    6. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
      Bernhard O.K., Kapp E.A., Simpson R.J.
      J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-385 AND ASN-658.
      Strain: C57BL/6.
      Tissue: Plasma.
    7. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-385.
    8. "An unusual cytokine:Ig-domain interaction revealed in the crystal structure of leukemia inhibitory factor (LIF) in complex with the LIF receptor."
      Huyton T., Zhang J.G., Luo C.S., Lou M.Z., Hilton D.J., Nicola N.A., Garrett T.P.
      Proc. Natl. Acad. Sci. U.S.A. 104:12737-12742(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 49-529 IN COMPLEX WITH HUMAN LIF, DISULFIDE BOND, GLYCOSYLATION AT ASN-164; ASN-199; ASN-238; ASN-261; ASN-385; ASN-402; ASN-421; ASN-440 AND ASN-453.

    Entry informationi

    Entry nameiLIFR_MOUSE
    AccessioniPrimary (citable) accession number: P42703
    Secondary accession number(s): Q5I0Y2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3