Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dynamin-related protein 1A

Gene

DRP1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-associated force-producing protein that is targeted to the forming cell plate during cytokinesis. Plays also a major role in plasma membrane maintenance and cell wall integrity with implications in vesicular trafficking, polar cell expansion, vascular formation, and other aspects of plant growth and development. Has a GTPase activity.5 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 49GTP1 Publication6
Nucleotide bindingi142 – 146GTPBy similarity5
Nucleotide bindingi212 – 217GTP1 Publication6
Nucleotide bindingi242 – 245GTP1 Publication4

GO - Molecular functioni

  • clathrin binding Source: TAIR
  • GTPase activity Source: TAIR
  • GTP binding Source: UniProtKB-KW
  • microtubule binding Source: GO_Central
  • protein histidine kinase binding Source: UniProtKB

GO - Biological processi

  • cell plate formation involved in plant-type cell wall biogenesis Source: TAIR
  • clathrin-dependent endocytosis Source: TAIR
  • cytokinesis by cell plate formation Source: TAIR
  • embryo development ending in seed dormancy Source: TAIR
  • mitochondrial fission Source: GO_Central
  • regulation of establishment of cell polarity Source: TAIR
  • root hair initiation Source: TAIR
  • trichome branching Source: TAIR
  • xylem and phloem pattern formation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Motor protein

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G42080-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin-related protein 1A
Alternative name(s):
Dynamin-like protein 1
Dynamin-like protein A
Protein RADIAL SWELLING 9
Gene namesi
Name:DRP1A
Synonyms:ADL1, ADL1A, AG68, RSW9
Ordered Locus Names:At5g42080
ORF Names:MJC20.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G42080.

Subcellular locationi

GO - Cellular componenti

  • cell plate Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • cytosol Source: TAIR
  • microtubule Source: TAIR
  • phragmoplast Source: UniProtKB-SubCell
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Vascular discontinuity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002065771 – 610Dynamin-related protein 1AAdd BLAST610

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP42697.
PRIDEiP42697.

PTM databases

iPTMnetiP42697.

Expressioni

Tissue specificityi

Ubiquitous. Expressed in leaves (at protein level).4 Publications

Gene expression databases

ExpressionAtlasiP42697. baseline and differential.
GenevisibleiP42697. AT.

Interactioni

Subunit structurei

Forms homodimer and may homooligomerize and heterooligomerize. Interacts with AGD3/VAN3. May interact with CALS1. Binds to AHK2.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AGD3Q5W7F24EBI-994234,EBI-994222
AHK2Q9C5U23EBI-994234,EBI-1100634
DRP2BQ9LQ554EBI-994234,EBI-2355848

GO - Molecular functioni

  • clathrin binding Source: TAIR
  • microtubule binding Source: GO_Central
  • protein histidine kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi19463. 8 interactors.
DIPiDIP-37642N.
IntActiP42697. 8 interactors.
MINTiMINT-7712636.
STRINGi3702.AT5G42080.1.

Structurei

Secondary structure

1610
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 10Combined sources7
Turni11 – 16Combined sources6
Beta strandi36 – 40Combined sources5
Helixi47 – 55Combined sources9
Beta strandi64 – 66Combined sources3
Beta strandi72 – 78Combined sources7
Beta strandi86 – 89Combined sources4
Helixi100 – 113Combined sources14
Beta strandi126 – 132Combined sources7
Beta strandi137 – 142Combined sources6
Helixi158 – 171Combined sources14
Beta strandi172 – 182Combined sources11
Helixi187 – 189Combined sources3
Helixi191 – 198Combined sources8
Beta strandi206 – 211Combined sources6
Helixi213 – 215Combined sources3
Helixi223 – 226Combined sources4
Beta strandi229 – 231Combined sources3
Helixi245 – 249Combined sources5
Helixi254 – 265Combined sources12
Turni269 – 271Combined sources3
Helixi272 – 277Combined sources6
Helixi280 – 296Combined sources17
Helixi302 – 310Combined sources9
Turni311 – 314Combined sources4
Helixi588 – 598Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T34X-ray2.40A/B1-325[»]
A/B579-606[»]
3T35X-ray3.59A/B/C/D1-325[»]
A/B/C/D579-606[»]
ProteinModelPortaliP42697.
SMRiP42697.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 300Dynamin-type GAdd BLAST270
Domaini518 – 610GEDPROSITE-ProRule annotationAdd BLAST93

Sequence similaritiesi

Contains 1 GED domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0446. Eukaryota.
COG0699. LUCA.
HOGENOMiHOG000161068.
InParanoidiP42697.
OMAiDKMREGS.
OrthoDBiEOG093605SP.
PhylomeDBiP42697.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P42697-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENLISLVNK IQRACTALGD HGDSSALPTL WDSLPAIAVV GGQSSGKSSV
60 70 80 90 100
LESIVGKDFL PRGSGIVTRR PLVLQLQKID DGTREYAEFL HLPRKKFTDF
110 120 130 140 150
AAVRKEIQDE TDRETGRSKA ISSVPIHLSI YSPNVVNLTL IDLPGLTKVA
160 170 180 190 200
VDGQSDSIVK DIENMVRSYI EKPNCIILAI SPANQDLATS DAIKISREVD
210 220 230 240 250
PSGDRTFGVL TKIDLMDKGT DAVEILEGRS FKLKYPWVGV VNRSQADINK
260 270 280 290 300
NVDMIAARKR EREYFSNTTE YRHLANKMGS EHLAKMLSKH LERVIKSRIP
310 320 330 340 350
GIQSLINKTV LELETELSRL GKPIAADAGG KLYSIMEICR LFDQIFKEHL
360 370 380 390 400
DGVRAGGEKV YNVFDNQLPA ALKRLQFDKQ LAMDNIRKLV TEADGYQPHL
410 420 430 440 450
IAPEQGYRRL IESSIVSIRG PAEASVDTVH AILKDLVHKS VNETVELKQY
460 470 480 490 500
PALRVEVTNA AIESLDKMRE GSKKATLQLV DMECSYLTVD FFRKLPQDVE
510 520 530 540 550
KGGNPTHSIF DRYNDSYLRR IGSNVLSYVN MVCAGLRNSI PKSIVYCQVR
560 570 580 590 600
EAKRSLLDHF FAELGTMDMK RLSSLLNEDP AIMERRSAIS KRLELYRAAQ
610
SEIDAVAWSK
Length:610
Mass (Da):68,172
Last modified:January 10, 2003 - v3
Checksum:iFE77E9EDBC603D91
GO
Isoform 2 (identifier: P42697-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     430-610: Missing.

Show »
Length:429
Mass (Da):47,533
Checksum:iFA88FAFE16D7DCE1
GO

Sequence cautioni

The sequence AAB63528 differs from that shown. Reason: Frameshift at positions 104, 132, 530 and 573.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50V → A in AAM19784 (PubMed:14593172).Curated1
Sequence conflicti50V → A in AAN46817 (PubMed:14593172).Curated1
Sequence conflicti167R → Q in AAB63528 (PubMed:9342876).Curated1
Sequence conflicti367Q → H in AAM65743 (Ref. 7) Curated1
Sequence conflicti423 – 424EA → KT in AAB63528 (PubMed:9342876).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009187430 – 610Missing in isoform 2. 2 PublicationsAdd BLAST181

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38614 mRNA. Translation: AAA84446.1.
L36939 mRNA. Translation: AAB63528.1. Frameshift.
AB017067 Genomic DNA. Translation: BAB08441.1.
CP002688 Genomic DNA. Translation: AED94763.1.
CP002688 Genomic DNA. Translation: AED94764.1.
AY094408 mRNA. Translation: AAM19784.1.
BT001063 mRNA. Translation: AAN46817.1.
BT025994 mRNA. Translation: ABG25083.1.
AY088201 mRNA. Translation: AAM65743.1.
AK230240 mRNA. Translation: BAF02045.1.
PIRiS59558.
RefSeqiNP_568602.3. NM_123573.3. [P42697-2]
NP_851120.1. NM_180789.3. [P42697-1]
UniGeneiAt.10365.

Genome annotation databases

EnsemblPlantsiAT5G42080.1; AT5G42080.1; AT5G42080. [P42697-1]
GeneIDi834213.
GrameneiAT5G42080.1; AT5G42080.1; AT5G42080.
KEGGiath:AT5G42080.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38614 mRNA. Translation: AAA84446.1.
L36939 mRNA. Translation: AAB63528.1. Frameshift.
AB017067 Genomic DNA. Translation: BAB08441.1.
CP002688 Genomic DNA. Translation: AED94763.1.
CP002688 Genomic DNA. Translation: AED94764.1.
AY094408 mRNA. Translation: AAM19784.1.
BT001063 mRNA. Translation: AAN46817.1.
BT025994 mRNA. Translation: ABG25083.1.
AY088201 mRNA. Translation: AAM65743.1.
AK230240 mRNA. Translation: BAF02045.1.
PIRiS59558.
RefSeqiNP_568602.3. NM_123573.3. [P42697-2]
NP_851120.1. NM_180789.3. [P42697-1]
UniGeneiAt.10365.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T34X-ray2.40A/B1-325[»]
A/B579-606[»]
3T35X-ray3.59A/B/C/D1-325[»]
A/B/C/D579-606[»]
ProteinModelPortaliP42697.
SMRiP42697.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19463. 8 interactors.
DIPiDIP-37642N.
IntActiP42697. 8 interactors.
MINTiMINT-7712636.
STRINGi3702.AT5G42080.1.

PTM databases

iPTMnetiP42697.

Proteomic databases

PaxDbiP42697.
PRIDEiP42697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G42080.1; AT5G42080.1; AT5G42080. [P42697-1]
GeneIDi834213.
GrameneiAT5G42080.1; AT5G42080.1; AT5G42080.
KEGGiath:AT5G42080.

Organism-specific databases

TAIRiAT5G42080.

Phylogenomic databases

eggNOGiKOG0446. Eukaryota.
COG0699. LUCA.
HOGENOMiHOG000161068.
InParanoidiP42697.
OMAiDKMREGS.
OrthoDBiEOG093605SP.
PhylomeDBiP42697.

Enzyme and pathway databases

BioCyciARA:AT5G42080-MONOMER.

Miscellaneous databases

PROiP42697.

Gene expression databases

ExpressionAtlasiP42697. baseline and differential.
GenevisibleiP42697. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDRP1A_ARATH
AccessioniPrimary (citable) accession number: P42697
Secondary accession number(s): Q0WLG2
, Q1EBU0, Q39118, Q8L9V5, Q8LPS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 10, 2003
Last modified: November 30, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.