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Protein

Dynamin-related protein 1A

Gene

DRP1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-associated force-producing protein that is targeted to the forming cell plate during cytokinesis. Plays also a major role in plasma membrane maintenance and cell wall integrity with implications in vesicular trafficking, polar cell expansion, vascular formation, and other aspects of plant growth and development. Has a GTPase activity.5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi44 – 496GTP1 Publication
Nucleotide bindingi142 – 1465GTPBy similarity
Nucleotide bindingi212 – 2176GTP1 Publication
Nucleotide bindingi242 – 2454GTP1 Publication

GO - Molecular functioni

  • clathrin binding Source: TAIR
  • GTPase activity Source: TAIR
  • GTP binding Source: UniProtKB-KW
  • protein histidine kinase binding Source: UniProtKB

GO - Biological processi

  • cell plate formation involved in plant-type cell wall biogenesis Source: TAIR
  • clathrin-mediated endocytosis Source: TAIR
  • cytokinesis by cell plate formation Source: TAIR
  • embryo development ending in seed dormancy Source: TAIR
  • trichome branching Source: TAIR
  • xylem and phloem pattern formation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Motor protein

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G42080-MONOMER.
ARA:GQT-1043-MONOMER.
ARA:GQT-1044-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin-related protein 1A
Alternative name(s):
Dynamin-like protein 1
Dynamin-like protein A
Protein RADIAL SWELLING 9
Gene namesi
Name:DRP1A
Synonyms:ADL1, ADL1A, AG68, RSW9
Ordered Locus Names:At5g42080
ORF Names:MJC20.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G42080.

Subcellular locationi

GO - Cellular componenti

  • cell plate Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • cytosol Source: TAIR
  • microtubule Source: TAIR
  • phragmoplast Source: UniProtKB-SubCell
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Vascular discontinuity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610Dynamin-related protein 1APRO_0000206577Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP42697.
PRIDEiP42697.

PTM databases

iPTMnetiP42697.

Expressioni

Tissue specificityi

Ubiquitous. Expressed in leaves (at protein level).4 Publications

Gene expression databases

ExpressionAtlasiP42697. baseline and differential.
GenevisibleiP42697. AT.

Interactioni

Subunit structurei

Forms homodimer and may homooligomerize and heterooligomerize. Interacts with AGD3/VAN3. May interact with CALS1. Binds to AHK2.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AGD3Q5W7F24EBI-994234,EBI-994222
AHK2Q9C5U23EBI-994234,EBI-1100634
DRP2BQ9LQ554EBI-994234,EBI-2355848

GO - Molecular functioni

  • clathrin binding Source: TAIR
  • protein histidine kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi19463. 8 interactions.
DIPiDIP-37642N.
IntActiP42697. 8 interactions.
MINTiMINT-7712636.
STRINGi3702.AT5G42080.1.

Structurei

Secondary structure

1
610
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 107Combined sources
Turni11 – 166Combined sources
Beta strandi36 – 405Combined sources
Helixi47 – 559Combined sources
Beta strandi64 – 663Combined sources
Beta strandi72 – 787Combined sources
Beta strandi86 – 894Combined sources
Helixi100 – 11314Combined sources
Beta strandi126 – 1327Combined sources
Beta strandi137 – 1426Combined sources
Helixi158 – 17114Combined sources
Beta strandi172 – 18211Combined sources
Helixi187 – 1893Combined sources
Helixi191 – 1988Combined sources
Beta strandi206 – 2116Combined sources
Helixi213 – 2153Combined sources
Helixi223 – 2264Combined sources
Beta strandi229 – 2313Combined sources
Helixi245 – 2495Combined sources
Helixi254 – 26512Combined sources
Turni269 – 2713Combined sources
Helixi272 – 2776Combined sources
Helixi280 – 29617Combined sources
Helixi302 – 3109Combined sources
Turni311 – 3144Combined sources
Helixi588 – 59811Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3T34X-ray2.40A/B1-325[»]
A/B579-606[»]
3T35X-ray3.59A/B/C/D1-325[»]
A/B/C/D579-606[»]
ProteinModelPortaliP42697.
SMRiP42697. Positions 1-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 300270Dynamin-type GAdd
BLAST
Domaini518 – 61093GEDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GED domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0446. Eukaryota.
COG0699. LUCA.
HOGENOMiHOG000161068.
InParanoidiP42697.
OMAiDKMREGS.
OrthoDBiEOG093605SP.
PhylomeDBiP42697.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P42697-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENLISLVNK IQRACTALGD HGDSSALPTL WDSLPAIAVV GGQSSGKSSV
60 70 80 90 100
LESIVGKDFL PRGSGIVTRR PLVLQLQKID DGTREYAEFL HLPRKKFTDF
110 120 130 140 150
AAVRKEIQDE TDRETGRSKA ISSVPIHLSI YSPNVVNLTL IDLPGLTKVA
160 170 180 190 200
VDGQSDSIVK DIENMVRSYI EKPNCIILAI SPANQDLATS DAIKISREVD
210 220 230 240 250
PSGDRTFGVL TKIDLMDKGT DAVEILEGRS FKLKYPWVGV VNRSQADINK
260 270 280 290 300
NVDMIAARKR EREYFSNTTE YRHLANKMGS EHLAKMLSKH LERVIKSRIP
310 320 330 340 350
GIQSLINKTV LELETELSRL GKPIAADAGG KLYSIMEICR LFDQIFKEHL
360 370 380 390 400
DGVRAGGEKV YNVFDNQLPA ALKRLQFDKQ LAMDNIRKLV TEADGYQPHL
410 420 430 440 450
IAPEQGYRRL IESSIVSIRG PAEASVDTVH AILKDLVHKS VNETVELKQY
460 470 480 490 500
PALRVEVTNA AIESLDKMRE GSKKATLQLV DMECSYLTVD FFRKLPQDVE
510 520 530 540 550
KGGNPTHSIF DRYNDSYLRR IGSNVLSYVN MVCAGLRNSI PKSIVYCQVR
560 570 580 590 600
EAKRSLLDHF FAELGTMDMK RLSSLLNEDP AIMERRSAIS KRLELYRAAQ
610
SEIDAVAWSK
Length:610
Mass (Da):68,172
Last modified:January 10, 2003 - v3
Checksum:iFE77E9EDBC603D91
GO
Isoform 2 (identifier: P42697-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     430-610: Missing.

Show »
Length:429
Mass (Da):47,533
Checksum:iFA88FAFE16D7DCE1
GO

Sequence cautioni

The sequence AAB63528 differs from that shown. Reason: Frameshift at positions 104, 132, 530 and 573. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501V → A in AAM19784 (PubMed:14593172).Curated
Sequence conflicti50 – 501V → A in AAN46817 (PubMed:14593172).Curated
Sequence conflicti167 – 1671R → Q in AAB63528 (PubMed:9342876).Curated
Sequence conflicti367 – 3671Q → H in AAM65743 (Ref. 7) Curated
Sequence conflicti423 – 4242EA → KT in AAB63528 (PubMed:9342876).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei430 – 610181Missing in isoform 2. 2 PublicationsVSP_009187Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38614 mRNA. Translation: AAA84446.1.
L36939 mRNA. Translation: AAB63528.1. Frameshift.
AB017067 Genomic DNA. Translation: BAB08441.1.
CP002688 Genomic DNA. Translation: AED94763.1.
CP002688 Genomic DNA. Translation: AED94764.1.
AY094408 mRNA. Translation: AAM19784.1.
BT001063 mRNA. Translation: AAN46817.1.
BT025994 mRNA. Translation: ABG25083.1.
AY088201 mRNA. Translation: AAM65743.1.
AK230240 mRNA. Translation: BAF02045.1.
PIRiS59558.
RefSeqiNP_568602.3. NM_123573.3. [P42697-2]
NP_851120.1. NM_180789.2. [P42697-1]
UniGeneiAt.10365.

Genome annotation databases

EnsemblPlantsiAT5G42080.1; AT5G42080.1; AT5G42080. [P42697-1]
GeneIDi834213.
KEGGiath:AT5G42080.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38614 mRNA. Translation: AAA84446.1.
L36939 mRNA. Translation: AAB63528.1. Frameshift.
AB017067 Genomic DNA. Translation: BAB08441.1.
CP002688 Genomic DNA. Translation: AED94763.1.
CP002688 Genomic DNA. Translation: AED94764.1.
AY094408 mRNA. Translation: AAM19784.1.
BT001063 mRNA. Translation: AAN46817.1.
BT025994 mRNA. Translation: ABG25083.1.
AY088201 mRNA. Translation: AAM65743.1.
AK230240 mRNA. Translation: BAF02045.1.
PIRiS59558.
RefSeqiNP_568602.3. NM_123573.3. [P42697-2]
NP_851120.1. NM_180789.2. [P42697-1]
UniGeneiAt.10365.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3T34X-ray2.40A/B1-325[»]
A/B579-606[»]
3T35X-ray3.59A/B/C/D1-325[»]
A/B/C/D579-606[»]
ProteinModelPortaliP42697.
SMRiP42697. Positions 1-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19463. 8 interactions.
DIPiDIP-37642N.
IntActiP42697. 8 interactions.
MINTiMINT-7712636.
STRINGi3702.AT5G42080.1.

PTM databases

iPTMnetiP42697.

Proteomic databases

PaxDbiP42697.
PRIDEiP42697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G42080.1; AT5G42080.1; AT5G42080. [P42697-1]
GeneIDi834213.
KEGGiath:AT5G42080.

Organism-specific databases

TAIRiAT5G42080.

Phylogenomic databases

eggNOGiKOG0446. Eukaryota.
COG0699. LUCA.
HOGENOMiHOG000161068.
InParanoidiP42697.
OMAiDKMREGS.
OrthoDBiEOG093605SP.
PhylomeDBiP42697.

Enzyme and pathway databases

BioCyciARA:AT5G42080-MONOMER.
ARA:GQT-1043-MONOMER.
ARA:GQT-1044-MONOMER.

Miscellaneous databases

PROiP42697.

Gene expression databases

ExpressionAtlasiP42697. baseline and differential.
GenevisibleiP42697. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDRP1A_ARATH
AccessioniPrimary (citable) accession number: P42697
Secondary accession number(s): Q0WLG2
, Q1EBU0, Q39118, Q8L9V5, Q8LPS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 10, 2003
Last modified: September 7, 2016
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.