P42690 (SRK4_SPOLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase isoform SRK4
EC=2.7.10.2

Gene names

Name:

SRK1

Organism

Spongilla lacustris (Freshwater sponge)

Taxonomic identifier

6055 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Porifera › Demospongiae › Ceractinomorpha › Haplosclerida › Spongillidae › Spongilla

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform SRK4 (identifier: P42690-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform SRK1 (identifier: P42686-1)

The sequence of this isoform can be found in the external entry P42686.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Tyrosine-protein kinase isoform SRK4		PRO_0000088157
Regions
	Domain	54 – 116	63	SH3
	Domain	122 – 214	93	SH2
	Domain	240 – 493	254	Protein kinase
	Nucleotide binding	246 – 254	9	ATP By similarity
Sites
	Active site	359	1	Proton acceptor By similarity
	Binding site	268	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform SRK4 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 8ABDF4A2546C280B	FASTA	506	57,562
	10 20 30 40 50 60 MGSCCSSQDG DGNGKATAGS TVDSHELSQS VKGKIKQPEP KPKPPPQVPP AQDVKYPIYV 70 80 90 100 110 120 GKYDYDSRTD DDLSFKKGDL MYIISTDEGD WWFARSKDTA GKEGYIPSNY VAEYKSLDAE 130 140 150 160 170 180 EWFFGQVKRV DAEKQLMMPF NNLGSFLIRD SDTTPGDFSL SVRDIDRVRH YRIKKLENGT 190 200 210 220 230 240 YFVTRRLTFQ SIQELVAYYT QQADGLCVNL KGPCMVMEKP QTAGLSKQAN EEWEIEKKQI 250 260 270 280 290 300 KLLRGLGAGQ FGEVWEGLWN GTTSVAVKTL KPGTMSIEEF LEEASIMKQL RHPKLIQLYA 310 320 330 340 350 360 VCTKEEPIYI VTELMKHGSL LEYLRGDGRS LKLPDLVDMC SQVASGMSYL EQQNYIHRDL 370 380 390 400 410 420 AARNILVGEH KICKVADFGL ARVIDEEIYE AKLGAKFPIK WTAPEAAMYS RFTIKSDVWS 430 440 450 460 470 480 FGIVLYEVIT YGRFPYPGMT NAQVLEQIQQ SYRMPRPMGC PEKLYAIMMD CWREDPASRP 490 500 TFETLSWQLE EFFTTGDDAG YKDMER
	Isoform SRK1 [UniParc]. See P42686.		–	–

References

[1]	"Multiple src-related kinase genes, srk1-4, in the fresh water sponge Spongilla lacustris." Ottilie S., Raulf F., Barnekow A., Hannig G., Schartl M. Oncogene 7:1625-1630(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61604 mRNA. Translation: CAA43801.1.
PIR	S24553.
3D structure databases
ProteinModelPortal	P42690.
SMR	P42690. Positions 58-133, 233-492.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.7.10.2. 5838.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR001452. SH3_domain. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. PR00109. TYRKINASE.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SRK4_SPOLA

Accession

Primary (citable) accession number: P42690

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)