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Protein

Tyrosine-protein kinase SPK-1

Gene
N/A
Organism
Girardia tigrina (Planarian) (Dugesia tigrina)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei248 – 2481ATPPROSITE-ProRule annotation
Active sitei342 – 3421Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi226 – 2349ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 2016.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase SPK-1 (EC:2.7.10.2)
OrganismiGirardia tigrina (Planarian) (Dugesia tigrina)
Taxonomic identifieri6162 [NCBI]
Taxonomic lineageiEukaryotaMetazoaPlatyhelminthesRhabditophoraSeriataTricladidaContinenticolaGeoplanoideaDugesiidaeGirardia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497Tyrosine-protein kinase SPK-1PRO_0000088138Add
BLAST

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliP42687.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 9462SH3PROSITE-ProRule annotationAdd
BLAST
Domaini100 – 200101SH2PROSITE-ProRule annotationAdd
BLAST
Domaini220 – 482263Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42687-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQKFSIKCK KQSKNKNTSK CQKIPKKAYE GPPGSYMVKA KYKYAASGDT
60 70 80 90 100
DISFEEKEIM YVLEQFDEFW LKVVKQKDNK EGLVPSNYVS KQDGSPQSVE
110 120 130 140 150
AWREIQRWEA EKSLMKIGLQ KGTYIIRPSR KENSYALSVR DFDEKKKICI
160 170 180 190 200
VKHFQIKTLQ DEKGISYSVN IRNFPNILTL IQFYEKNGIG NTHIPLTDPM
210 220 230 240 250
PDNYQPPVHF QDIEINRENI EILNEIGRGF FGSVHRAKWG RSYEVAAKML
260 270 280 290 300
QSSKAEREKF VLEAKIMHKL RHRKIVELLG VCTEPQDMPM LIIVEYMKNG
310 320 330 340 350
SLKEYLKTPD GKKTNLNQMV HMMAEISEGM AYLESEKVVH RDLRADNILV
360 370 380 390 400
ANDLTRKVAD FGLTELTDGS LGDQEKKTLR FPYKWTAPEA AKSKVFTSKS
410 420 430 440 450
DVWSYGIVMF EILTWASSPY PDIPAKEVIE KVSKGYRMPN PEKFITGVCC
460 470 480 490
PDEIYKIMIW CWDANPEKRP TFLVLQEKMD LLIVDTLTNN AYYSHSK
Length:497
Mass (Da):57,447
Last modified:November 1, 1995 - v1
Checksum:i412FD10588997524
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti212 – 2121D → A.
Natural varianti235 – 2351H → D.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75310 mRNA. Translation: CAA53058.1.
PIRiS43532.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75310 mRNA. Translation: CAA53058.1.
PIRiS43532.

3D structure databases

ProteinModelPortaliP42687.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.7.10.2. 2016.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure and expression of Spk-1, an src-related gene product found in the planarian Dugesia (G) tigrina."
    Burgaya F., Garcia-Fernandez J., Riutort M., Baguna J., Salo E.
    Oncogene 9:1267-1272(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiSPK1_GIRTI
AccessioniPrimary (citable) accession number: P42687
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 1, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

It is uncertain whether Met-1 is the initiator.Curated

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.