P42687 (SPK1_DUGTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Tyrosine-protein kinase SPK-1 EC=2.7.10.2
Organism	Dugesia tigrina (Planarian) (Girardia tigrina)
Taxonomic identifier	6162 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Platyhelminthes › Turbellaria › Seriata › Tricladida › Continenticola › Geoplanoidea › Dugesiidae › Girardia

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
Caution	It is uncertain whether Met-1 is the initiator.

Ontologies

Keywords
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Tyrosine-protein kinase SPK-1		PRO_0000088138
Regions
	Domain	33 – 94	62	SH3
	Domain	100 – 200	101	SH2
	Domain	220 – 482	263	Protein kinase
	Nucleotide binding	226 – 234	9	ATP By similarity
Sites
	Active site	342	1	Proton acceptor By similarity
	Binding site	248	1	ATP By similarity
Natural variations
	Natural variant	212	1	D → A.
	Natural variant	235	1	H → D.

Sequences

	Sequence		Length	Mass (Da)
	P42687 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 412FD10588997524	FASTA	497	57,447
	10 20 30 40 50 60 MGQKFSIKCK KQSKNKNTSK CQKIPKKAYE GPPGSYMVKA KYKYAASGDT DISFEEKEIM 70 80 90 100 110 120 YVLEQFDEFW LKVVKQKDNK EGLVPSNYVS KQDGSPQSVE AWREIQRWEA EKSLMKIGLQ 130 140 150 160 170 180 KGTYIIRPSR KENSYALSVR DFDEKKKICI VKHFQIKTLQ DEKGISYSVN IRNFPNILTL 190 200 210 220 230 240 IQFYEKNGIG NTHIPLTDPM PDNYQPPVHF QDIEINRENI EILNEIGRGF FGSVHRAKWG 250 260 270 280 290 300 RSYEVAAKML QSSKAEREKF VLEAKIMHKL RHRKIVELLG VCTEPQDMPM LIIVEYMKNG 310 320 330 340 350 360 SLKEYLKTPD GKKTNLNQMV HMMAEISEGM AYLESEKVVH RDLRADNILV ANDLTRKVAD 370 380 390 400 410 420 FGLTELTDGS LGDQEKKTLR FPYKWTAPEA AKSKVFTSKS DVWSYGIVMF EILTWASSPY 430 440 450 460 470 480 PDIPAKEVIE KVSKGYRMPN PEKFITGVCC PDEIYKIMIW CWDANPEKRP TFLVLQEKMD 490 LLIVDTLTNN AYYSHSK

References

[1]	"Structure and expression of Spk-1, an src-related gene product found in the planarian Dugesia (G) tigrina." Burgaya F., Garcia-Fernandez J., Riutort M., Baguna J., Salo E. Oncogene 9:1267-1272(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75310 mRNA. Translation: CAA53058.1.
PIR	S43532.
3D structure databases
ProteinModelPortal	P42687.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.7.10.2. 2016.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR001452. SH3_domain. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00109. TYRKINASE.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPK1_DUGTI

Accession

Primary (citable) accession number: P42687

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)