SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P42683

- LCK_CHICK

UniProt

P42683 - LCK_CHICK

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Proto-oncogene tyrosine-protein kinase LCK

Gene
LCK
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways By similarity.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulationi

Inhibited by tyrosine phosphorylation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei272 – 2721ATP By similarity
Active sitei363 – 3631Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi250 – 2589ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  3. protein serine/threonine phosphatase activity Source: UniProtKB
  4. protein tyrosine kinase activity Source: UniProtKB
  5. SH2 domain binding Source: UniProtKB

GO - Biological processi

  1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  2. cellular zinc ion homeostasis Source: UniProtKB
  3. dephosphorylation Source: GOC
  4. peptidyl-tyrosine phosphorylation Source: GOC
  5. positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
  6. positive regulation of T cell activation Source: UniProtKB
  7. response to drug Source: UniProtKB
  8. T cell differentiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 1306.

Names & Taxonomyi

Protein namesi
Recommended name:
Proto-oncogene tyrosine-protein kinase LCK (EC:2.7.10.2)
Alternative name(s):
Protein-tyrosine kinase C-TKL
p56tk1
Gene namesi
Name:LCK
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. membrane raft Source: UniProtKB
  2. pericentriolar material Source: UniProtKB
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed Inferred
Chaini2 – 508507Proto-oncogene tyrosine-protein kinase LCKPRO_0000088128Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine By similarity
Lipidationi3 – 31S-palmitoyl cysteine By similarity
Lipidationi5 – 51S-palmitoyl cysteine By similarity
Modified residuei393 – 3931Phosphotyrosine; by autocatalysis By similarity
Modified residuei504 – 5041Phosphotyrosine By similarity

Post-translational modificationi

Phosphorylated on Tyr-393, which increases enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-504, presumably by CSK, which decreases activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-393 by PTPN2 negatively regulates T-cells differentiation By similarity.

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiP42683.

Interactioni

Subunit structurei

Binds to the cytoplasmic domain of cell surface receptors, such as CD4, CD8 By similarity.

Structurei

3D structure databases

ProteinModelPortaliP42683.
SMRiP42683. Positions 7-33, 64-508.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 12061SH3Add
BLAST
Domaini126 – 22398SH2Add
BLAST
Domaini244 – 497254Protein kinaseAdd
BLAST

Sequence similaritiesi

Contains 1 SH2 domain.
Contains 1 SH3 domain.

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

HOVERGENiHBG008761.
PhylomeDBiP42683.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42683-1 [UniParc]FASTAAdd to Basket

« Hide

MGCCCSSDYD EDWIENIDIC EHCNYPIDPD SKRQQLIRNV SEVRDPLVSY    50
EAMSPPCSPL QDKLVVALYD YEPTHDGDLG LKQGEKLRVL EESGEWWRAQ 100
SLTTGQEGLI PHNFVAMVNS LEPEPWFFKN LSRKNAEARL LASGNTHGSF 150
LIRESETSKG SYSLSVRDFD QNQGETVKHY KIRNMDNGGY YISPRVTFSS 200
LHELVEYYSS SSDGLCTRLG KPCRTQKPQK PWWQDEWEVP RESLKLVEKL 250
GAGQFGEVWM GFYNGHTKVA IKNLKQGSMS PSAFLAEANL MKNLQHPRLV 300
RLYAVVTKEP IYIITEYMEK GSLVDFLKTS EGIKLSINKL LDMAAQIAEG 350
MAFIEAKNYI HRDLRAANIL VSEALCCKIA DFGLARLIED NEYTAREGAK 400
FPIKWTAPEA INYGTFTIKS DVWSFGILLT EIVTYGRIPY PGMTNPEVIQ 450
NLERGYRMPQ PDNCPQELYE LMMQCWKEQP EERPTFEYMK SVLEDFFTAT 500
EGQYQQQP 508
Length:508
Mass (Da):58,140
Last modified:January 23, 2007 - v2
Checksum:iBC83C4E61CB66170
GO

Sequence cautioni

The sequence AAA49081.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60380 mRNA. Translation: CAA42930.1.
M85043 mRNA. Translation: AAA49003.1.
J03579 mRNA. Translation: AAA49081.1. Different initiation.
PIRiA42126. A39939.
UniGeneiGga.11320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60380 mRNA. Translation: CAA42930.1 .
M85043 mRNA. Translation: AAA49003.1 .
J03579 mRNA. Translation: AAA49081.1 . Different initiation.
PIRi A42126. A39939.
UniGenei Gga.11320.

3D structure databases

ProteinModelPortali P42683.
SMRi P42683. Positions 7-33, 64-508.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P42683.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG008761.
PhylomeDBi P42683.

Enzyme and pathway databases

BRENDAi 2.7.10.2. 1306.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Gaertner T., Khnel H., Strebhardt K., Ruebsamen-Waigmann H.
    Submitted (AUG-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  2. "tkl is the avian homolog of the mammalian lck tyrosine protein kinase gene."
    Chow L., Ratcliffe M., Veillette A.
    Mol. Cell. Biol. 12:1226-1233(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-89.
  3. "Additional member of the protein-tyrosine kinase family: the src- and lck-related protooncogene c-tkl."
    Strebhardt K., Mullins J.I., Bruck C., Ruebsamen-Waigmann H.
    Proc. Natl. Acad. Sci. U.S.A. 84:8778-8782(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 47-508.

Entry informationi

Entry nameiLCK_CHICK
AccessioniPrimary (citable) accession number: P42683
Secondary accession number(s): Q53WS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi