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P42683

- LCK_CHICK

UniProt

P42683 - LCK_CHICK

Protein

Proto-oncogene tyrosine-protein kinase LCK

Gene

LCK

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Enzyme regulationi

    Inhibited by tyrosine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei272 – 2721ATPPROSITE-ProRule annotation
    Active sitei363 – 3631Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi250 – 2589ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    3. protein serine/threonine phosphatase activity Source: UniProtKB
    4. protein tyrosine kinase activity Source: UniProtKB
    5. SH2 domain binding Source: UniProtKB

    GO - Biological processi

    1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
    2. cellular zinc ion homeostasis Source: UniProtKB
    3. dephosphorylation Source: GOC
    4. peptidyl-tyrosine phosphorylation Source: GOC
    5. positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
    6. positive regulation of T cell activation Source: UniProtKB
    7. response to drug Source: UniProtKB
    8. T cell differentiation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 1306.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proto-oncogene tyrosine-protein kinase LCK (EC:2.7.10.2)
    Alternative name(s):
    Protein-tyrosine kinase C-TKL
    p56tk1
    Gene namesi
    Name:LCK
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. membrane raft Source: UniProtKB
    2. pericentriolar material Source: UniProtKB
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedCurated
    Chaini2 – 508507Proto-oncogene tyrosine-protein kinase LCKPRO_0000088128Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Lipidationi3 – 31S-palmitoyl cysteineBy similarity
    Lipidationi5 – 51S-palmitoyl cysteineBy similarity
    Modified residuei393 – 3931Phosphotyrosine; by autocatalysisBy similarity
    Modified residuei504 – 5041PhosphotyrosineBy similarity

    Post-translational modificationi

    Phosphorylated on Tyr-393, which increases enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-504, presumably by CSK, which decreases activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-393 by PTPN2 negatively regulates T-cells differentiation By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate, Phosphoprotein

    Proteomic databases

    PRIDEiP42683.

    Interactioni

    Subunit structurei

    Binds to the cytoplasmic domain of cell surface receptors, such as CD4, CD8.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP42683.
    SMRiP42683. Positions 7-33, 64-508.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini60 – 12061SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini126 – 22398SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini244 – 497254Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain, SH3 domain

    Phylogenomic databases

    HOVERGENiHBG008761.
    PhylomeDBiP42683.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P42683-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCCCSSDYD EDWIENIDIC EHCNYPIDPD SKRQQLIRNV SEVRDPLVSY    50
    EAMSPPCSPL QDKLVVALYD YEPTHDGDLG LKQGEKLRVL EESGEWWRAQ 100
    SLTTGQEGLI PHNFVAMVNS LEPEPWFFKN LSRKNAEARL LASGNTHGSF 150
    LIRESETSKG SYSLSVRDFD QNQGETVKHY KIRNMDNGGY YISPRVTFSS 200
    LHELVEYYSS SSDGLCTRLG KPCRTQKPQK PWWQDEWEVP RESLKLVEKL 250
    GAGQFGEVWM GFYNGHTKVA IKNLKQGSMS PSAFLAEANL MKNLQHPRLV 300
    RLYAVVTKEP IYIITEYMEK GSLVDFLKTS EGIKLSINKL LDMAAQIAEG 350
    MAFIEAKNYI HRDLRAANIL VSEALCCKIA DFGLARLIED NEYTAREGAK 400
    FPIKWTAPEA INYGTFTIKS DVWSFGILLT EIVTYGRIPY PGMTNPEVIQ 450
    NLERGYRMPQ PDNCPQELYE LMMQCWKEQP EERPTFEYMK SVLEDFFTAT 500
    EGQYQQQP 508
    Length:508
    Mass (Da):58,140
    Last modified:January 23, 2007 - v2
    Checksum:iBC83C4E61CB66170
    GO

    Sequence cautioni

    The sequence AAA49081.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60380 mRNA. Translation: CAA42930.1.
    M85043 mRNA. Translation: AAA49003.1.
    J03579 mRNA. Translation: AAA49081.1. Different initiation.
    PIRiA42126. A39939.
    UniGeneiGga.11320.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60380 mRNA. Translation: CAA42930.1 .
    M85043 mRNA. Translation: AAA49003.1 .
    J03579 mRNA. Translation: AAA49081.1 . Different initiation.
    PIRi A42126. A39939.
    UniGenei Gga.11320.

    3D structure databases

    ProteinModelPortali P42683.
    SMRi P42683. Positions 7-33, 64-508.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P42683.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG008761.
    PhylomeDBi P42683.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 1306.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Gaertner T., Khnel H., Strebhardt K., Ruebsamen-Waigmann H.
      Submitted (AUG-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Spleen.
    2. "tkl is the avian homolog of the mammalian lck tyrosine protein kinase gene."
      Chow L., Ratcliffe M., Veillette A.
      Mol. Cell. Biol. 12:1226-1233(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-89.
    3. "Additional member of the protein-tyrosine kinase family: the src- and lck-related protooncogene c-tkl."
      Strebhardt K., Mullins J.I., Bruck C., Ruebsamen-Waigmann H.
      Proc. Natl. Acad. Sci. U.S.A. 84:8778-8782(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 47-508.

    Entry informationi

    Entry nameiLCK_CHICK
    AccessioniPrimary (citable) accession number: P42683
    Secondary accession number(s): Q53WS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3