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Protein

Megakaryocyte-associated tyrosine-protein kinase

Gene

MATK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei262ATPPROSITE-ProRule annotation1
Active sitei352Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi241 – 249ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: GO_Central
  • cell migration Source: GO_Central
  • cell proliferation Source: ProtInc
  • ERBB2 signaling pathway Source: Reactome
  • innate immune response Source: GO_Central
  • mesoderm development Source: ProtInc
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • positive regulation of cell proliferation Source: ProtInc
  • protein phosphorylation Source: ProtInc
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS00209-MONOMER.
BRENDAi2.7.10.2. 2681.
ReactomeiR-HSA-8863795. Downregulation of ERBB2 signaling.
SignaLinkiP42679.
SIGNORiP42679.

Names & Taxonomyi

Protein namesi
Recommended name:
Megakaryocyte-associated tyrosine-protein kinase (EC:2.7.10.2)
Alternative name(s):
CSK homologous kinase
Short name:
CHK
Hematopoietic consensus tyrosine-lacking kinase
Protein kinase HYL
Tyrosine-protein kinase CTK
Gene namesi
Name:MATK
Synonyms:CTK, HYL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:6906. MATK.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Membrane 1 Publication

  • Note: In platelets, 90% of MATK localizes to the membrane fraction, and translocates to the cytoskeleton upon thrombin stimulation.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi4145.
OpenTargetsiENSG00000007264.
PharmGKBiPA30649.

Chemistry databases

ChEMBLiCHEMBL4175.
GuidetoPHARMACOLOGYi2101.

Polymorphism and mutation databases

BioMutaiMATK.
DMDMi1169123.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880731 – 507Megakaryocyte-associated tyrosine-protein kinaseAdd BLAST507

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei501PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP42679.
MaxQBiP42679.
PaxDbiP42679.
PeptideAtlasiP42679.
PRIDEiP42679.

PTM databases

iPTMnetiP42679.
PhosphoSitePlusiP42679.

Expressioni

Tissue specificityi

Expressed in various myeloid cell lines, detected in brain and lung.

Gene expression databases

BgeeiENSG00000007264.
CleanExiHS_MATK.
ExpressionAtlasiP42679. baseline and differential.
GenevisibleiP42679. HS.

Organism-specific databases

HPAiHPA004847.

Interactioni

Subunit structurei

Interacts with KIT.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ARP102754EBI-751664,EBI-608057
ERBB2P046262EBI-751664,EBI-641062
HSP90AB1P082382EBI-751664,EBI-352572
LRRK2Q5S0072EBI-751664,EBI-5323863

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110315. 13 interactors.
IntActiP42679. 11 interactors.
MINTiMINT-1466004.
STRINGi9606.ENSP00000378485.

Chemistry databases

BindingDBiP42679.

Structurei

Secondary structure

1507
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi52 – 57Combined sources6
Beta strandi59 – 61Combined sources3
Beta strandi74 – 79Combined sources6
Beta strandi83 – 91Combined sources9
Turni92 – 94Combined sources3
Beta strandi97 – 101Combined sources5
Helixi102 – 104Combined sources3
Beta strandi105 – 107Combined sources3
Beta strandi123 – 126Combined sources4
Helixi129 – 135Combined sources7
Beta strandi144 – 148Combined sources5
Beta strandi150 – 152Combined sources3
Beta strandi156 – 162Combined sources7
Beta strandi165 – 174Combined sources10
Beta strandi177 – 188Combined sources12
Helixi189 – 198Combined sources10
Beta strandi203 – 205Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JWOX-ray2.50A117-213[»]
1X6GNMR-A41-108[»]
3US4X-ray1.50A117-213[»]
ProteinModelPortaliP42679.
SMRiP42679.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42679.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 110SH3PROSITE-ProRule annotationAdd BLAST53
Domaini122 – 211SH2PROSITE-ProRule annotationAdd BLAST90
Domaini235 – 482Protein kinasePROSITE-ProRule annotationAdd BLAST248

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000119011.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiP42679.
KOiK08888.
PhylomeDBiP42679.
TreeFamiTF351634.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P42679-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGRGSLVSW RAFHGCDSAE ELPRVSPRFL RAWHPPPVSA RMPTRRWAPG
60 70 80 90 100
TQCITKCEHT RPKPGELAFR KGDVVTILEA CENKSWYRVK HHTSGQEGLL
110 120 130 140 150
AAGALREREA LSADPKLSLM PWFHGKISGQ EAVQQLQPPE DGLFLVRESA
160 170 180 190 200
RHPGDYVLCV SFGRDVIHYR VLHRDGHLTI DEAVFFCNLM DMVEHYSKDK
210 220 230 240 250
GAICTKLVRP KRKHGTKSAE EELARAGWLL NLQHLTLGAQ IGEGEFGAVL
260 270 280 290 300
QGEYLGQKVA VKNIKCDVTA QAFLDETAVM TKMQHENLVR LLGVILHQGL
310 320 330 340 350
YIVMEHVSKG NLVNFLRTRG RALVNTAQLL QFSLHVAEGM EYLESKKLVH
360 370 380 390 400
RDLAARNILV SEDLVAKVSD FGLAKAERKG LDSSRLPVKW TAPEALKHGK
410 420 430 440 450
FTSKSDVWSF GVLLWEVFSY GRAPYPKMSL KEVSEAVEKG YRMEPPEGCP
460 470 480 490 500
GPVHVLMSSC WEAEPARRPP FRKLAEKLAR ELRSAGAPAS VSGQDADGST

SPRSQEP
Length:507
Mass (Da):56,469
Last modified:November 1, 1995 - v1
Checksum:i85721C6E024575EF
GO
Isoform 2 (identifier: P42679-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-24: MAGRGSLVSWRAFHGCDSAEELPR → MQGHFPAERREGRPRRGTRGQQQLL

Note: No experimental confirmation available.
Show »
Length:508
Mass (Da):56,798
Checksum:iCEFD5D74DAC082E5
GO
Isoform 3 (identifier: P42679-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: Missing.

Show »
Length:466
Mass (Da):51,899
Checksum:iB2C4DFFE995D5FEE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107 – 108ER → DG in AAA16703 (PubMed:8134117).Curated2
Sequence conflicti400Missing in AAA16703 (PubMed:8134117).Curated1
Sequence conflicti466 – 507ARRPP…RSQEP → PAGHPSANWPRSWPGSYAVQ VPQPPSQGRTPTVHLAPKPG ALTPPGGPWPQRTERVESAA WGH in AAA16703 (PubMed:8134117).CuratedAdd BLAST42

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_041679354A → T in an ovarian mucinous carcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_041680496A → T.1 PublicationCorresponds to variant rs35351680dbSNPEnsembl.1
Natural variantiVAR_041681503R → Q in a colorectal adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant rs778726488dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0442771 – 41Missing in isoform 3. 1 PublicationAdd BLAST41
Alternative sequenceiVSP_0431231 – 24MAGRG…EELPR → MQGHFPAERREGRPRRGTRG QQQLL in isoform 2. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L18974 mRNA. Translation: AAA16703.1.
X77278 mRNA. Translation: CAA54493.1.
S75164
, S75145, S75147, S75166, S75168, S75151, S75153, S75155, S75156, S75158, S75159, S75162 Genomic DNA. Translation: AAC60645.1.
AK055395 mRNA. Translation: BAG51511.1.
AL137754 mRNA. Translation: CAB70906.2.
AC005777 Genomic DNA. Translation: AAC62843.1.
CH471139 Genomic DNA. Translation: EAW69285.1.
BC000114 mRNA. Translation: AAH00114.1.
BC003109 mRNA. Translation: AAH03109.1.
CCDSiCCDS12113.1. [P42679-2]
CCDS12114.1. [P42679-1]
CCDS42468.1. [P42679-3]
PIRiA49865.
A55625.
RefSeqiNP_002369.2. NM_002378.3. [P42679-2]
NP_647611.1. NM_139354.2. [P42679-3]
NP_647612.1. NM_139355.2. [P42679-1]
XP_011526320.1. XM_011528018.1. [P42679-1]
UniGeneiHs.631845.

Genome annotation databases

EnsembliENST00000310132; ENSP00000308734; ENSG00000007264. [P42679-1]
ENST00000395040; ENSP00000378481; ENSG00000007264. [P42679-3]
ENST00000395045; ENSP00000378485; ENSG00000007264. [P42679-2]
ENST00000619596; ENSP00000483213; ENSG00000007264. [P42679-2]
GeneIDi4145.
KEGGihsa:4145.
UCSCiuc002lyt.4. human. [P42679-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L18974 mRNA. Translation: AAA16703.1.
X77278 mRNA. Translation: CAA54493.1.
S75164
, S75145, S75147, S75166, S75168, S75151, S75153, S75155, S75156, S75158, S75159, S75162 Genomic DNA. Translation: AAC60645.1.
AK055395 mRNA. Translation: BAG51511.1.
AL137754 mRNA. Translation: CAB70906.2.
AC005777 Genomic DNA. Translation: AAC62843.1.
CH471139 Genomic DNA. Translation: EAW69285.1.
BC000114 mRNA. Translation: AAH00114.1.
BC003109 mRNA. Translation: AAH03109.1.
CCDSiCCDS12113.1. [P42679-2]
CCDS12114.1. [P42679-1]
CCDS42468.1. [P42679-3]
PIRiA49865.
A55625.
RefSeqiNP_002369.2. NM_002378.3. [P42679-2]
NP_647611.1. NM_139354.2. [P42679-3]
NP_647612.1. NM_139355.2. [P42679-1]
XP_011526320.1. XM_011528018.1. [P42679-1]
UniGeneiHs.631845.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JWOX-ray2.50A117-213[»]
1X6GNMR-A41-108[»]
3US4X-ray1.50A117-213[»]
ProteinModelPortaliP42679.
SMRiP42679.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110315. 13 interactors.
IntActiP42679. 11 interactors.
MINTiMINT-1466004.
STRINGi9606.ENSP00000378485.

Chemistry databases

BindingDBiP42679.
ChEMBLiCHEMBL4175.
GuidetoPHARMACOLOGYi2101.

PTM databases

iPTMnetiP42679.
PhosphoSitePlusiP42679.

Polymorphism and mutation databases

BioMutaiMATK.
DMDMi1169123.

Proteomic databases

EPDiP42679.
MaxQBiP42679.
PaxDbiP42679.
PeptideAtlasiP42679.
PRIDEiP42679.

Protocols and materials databases

DNASUi4145.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310132; ENSP00000308734; ENSG00000007264. [P42679-1]
ENST00000395040; ENSP00000378481; ENSG00000007264. [P42679-3]
ENST00000395045; ENSP00000378485; ENSG00000007264. [P42679-2]
ENST00000619596; ENSP00000483213; ENSG00000007264. [P42679-2]
GeneIDi4145.
KEGGihsa:4145.
UCSCiuc002lyt.4. human. [P42679-1]

Organism-specific databases

CTDi4145.
DisGeNETi4145.
GeneCardsiMATK.
HGNCiHGNC:6906. MATK.
HPAiHPA004847.
MIMi600038. gene.
neXtProtiNX_P42679.
OpenTargetsiENSG00000007264.
PharmGKBiPA30649.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000119011.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiP42679.
KOiK08888.
PhylomeDBiP42679.
TreeFamiTF351634.

Enzyme and pathway databases

BioCyciZFISH:HS00209-MONOMER.
BRENDAi2.7.10.2. 2681.
ReactomeiR-HSA-8863795. Downregulation of ERBB2 signaling.
SignaLinkiP42679.
SIGNORiP42679.

Miscellaneous databases

EvolutionaryTraceiP42679.
GeneWikiiMegakaryocyte-associated_tyrosine_kinase.
GenomeRNAii4145.
PROiP42679.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000007264.
CleanExiHS_MATK.
ExpressionAtlasiP42679. baseline and differential.
GenevisibleiP42679. HS.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMATK_HUMAN
AccessioniPrimary (citable) accession number: P42679
Secondary accession number(s): B3KNZ9, Q9NST8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 179 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.