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Protein

Megakaryocyte-associated tyrosine-protein kinase

Gene

MATK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei262ATPPROSITE-ProRule annotation1
Active sitei352Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi241 – 249ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: GO_Central
  • cell proliferation Source: ProtInc
  • ERBB2 signaling pathway Source: Reactome
  • innate immune response Source: GO_Central
  • mesoderm development Source: ProtInc
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • positive regulation of cell proliferation Source: ProtInc
  • protein phosphorylation Source: ProtInc
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 2681
ReactomeiR-HSA-8863795 Downregulation of ERBB2 signaling
SignaLinkiP42679
SIGNORiP42679

Names & Taxonomyi

Protein namesi
Recommended name:
Megakaryocyte-associated tyrosine-protein kinase (EC:2.7.10.2)
Alternative name(s):
CSK homologous kinase
Short name:
CHK
Hematopoietic consensus tyrosine-lacking kinase
Protein kinase HYL
Tyrosine-protein kinase CTK
Gene namesi
Name:MATK
Synonyms:CTK, HYL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000007264.13
HGNCiHGNC:6906 MATK
MIMi600038 gene
neXtProtiNX_P42679

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi4145
OpenTargetsiENSG00000007264
PharmGKBiPA30649

Chemistry databases

ChEMBLiCHEMBL4175
GuidetoPHARMACOLOGYi2101

Polymorphism and mutation databases

BioMutaiMATK
DMDMi1169123

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880731 – 507Megakaryocyte-associated tyrosine-protein kinaseAdd BLAST507

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei501PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP42679
MaxQBiP42679
PaxDbiP42679
PeptideAtlasiP42679
PRIDEiP42679

PTM databases

iPTMnetiP42679
PhosphoSitePlusiP42679

Expressioni

Tissue specificityi

Expressed in various myeloid cell lines, detected in brain and lung.

Gene expression databases

BgeeiENSG00000007264
CleanExiHS_MATK
ExpressionAtlasiP42679 baseline and differential
GenevisibleiP42679 HS

Organism-specific databases

HPAiHPA004847

Interactioni

Subunit structurei

Interacts with KIT.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110315, 14 interactors
IntActiP42679, 11 interactors
MINTiP42679
STRINGi9606.ENSP00000378485

Chemistry databases

BindingDBiP42679

Structurei

Secondary structure

1507
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi52 – 57Combined sources6
Beta strandi59 – 61Combined sources3
Beta strandi74 – 79Combined sources6
Beta strandi83 – 91Combined sources9
Turni92 – 94Combined sources3
Beta strandi97 – 101Combined sources5
Helixi102 – 104Combined sources3
Beta strandi105 – 107Combined sources3
Beta strandi123 – 126Combined sources4
Helixi129 – 135Combined sources7
Beta strandi144 – 148Combined sources5
Beta strandi150 – 152Combined sources3
Beta strandi156 – 162Combined sources7
Beta strandi165 – 174Combined sources10
Beta strandi177 – 188Combined sources12
Helixi189 – 198Combined sources10
Beta strandi203 – 205Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JWOX-ray2.50A117-213[»]
1X6GNMR-A41-108[»]
3US4X-ray1.50A117-213[»]
ProteinModelPortaliP42679
SMRiP42679
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42679

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 110SH3PROSITE-ProRule annotationAdd BLAST63
Domaini122 – 211SH2PROSITE-ProRule annotationAdd BLAST90
Domaini235 – 482Protein kinasePROSITE-ProRule annotationAdd BLAST248

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG0197 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00760000119011
HOGENOMiHOG000233858
HOVERGENiHBG008761
InParanoidiP42679
KOiK08888
OMAiKAGWLLN
PhylomeDBiP42679
TreeFamiTF351634

Family and domain databases

CDDicd09937 SH2_csk_like, 1 hit
Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR035027 Csk-like_SH2
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
PF00017 SH2, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00401 SH2DOMAIN
PR00109 TYRKINASE
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00326 SH3, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
SSF55550 SSF55550, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS50001 SH2, 1 hit
PS50002 SH3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P42679-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGRGSLVSW RAFHGCDSAE ELPRVSPRFL RAWHPPPVSA RMPTRRWAPG
60 70 80 90 100
TQCITKCEHT RPKPGELAFR KGDVVTILEA CENKSWYRVK HHTSGQEGLL
110 120 130 140 150
AAGALREREA LSADPKLSLM PWFHGKISGQ EAVQQLQPPE DGLFLVRESA
160 170 180 190 200
RHPGDYVLCV SFGRDVIHYR VLHRDGHLTI DEAVFFCNLM DMVEHYSKDK
210 220 230 240 250
GAICTKLVRP KRKHGTKSAE EELARAGWLL NLQHLTLGAQ IGEGEFGAVL
260 270 280 290 300
QGEYLGQKVA VKNIKCDVTA QAFLDETAVM TKMQHENLVR LLGVILHQGL
310 320 330 340 350
YIVMEHVSKG NLVNFLRTRG RALVNTAQLL QFSLHVAEGM EYLESKKLVH
360 370 380 390 400
RDLAARNILV SEDLVAKVSD FGLAKAERKG LDSSRLPVKW TAPEALKHGK
410 420 430 440 450
FTSKSDVWSF GVLLWEVFSY GRAPYPKMSL KEVSEAVEKG YRMEPPEGCP
460 470 480 490 500
GPVHVLMSSC WEAEPARRPP FRKLAEKLAR ELRSAGAPAS VSGQDADGST

SPRSQEP
Length:507
Mass (Da):56,469
Last modified:November 1, 1995 - v1
Checksum:i85721C6E024575EF
GO
Isoform 2 (identifier: P42679-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-24: MAGRGSLVSWRAFHGCDSAEELPR → MQGHFPAERREGRPRRGTRGQQQLL

Note: No experimental confirmation available.
Show »
Length:508
Mass (Da):56,798
Checksum:iCEFD5D74DAC082E5
GO
Isoform 3 (identifier: P42679-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: Missing.

Show »
Length:466
Mass (Da):51,899
Checksum:iB2C4DFFE995D5FEE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107 – 108ER → DG in AAA16703 (PubMed:8134117).Curated2
Sequence conflicti400Missing in AAA16703 (PubMed:8134117).Curated1
Sequence conflicti466 – 507ARRPP…RSQEP → PAGHPSANWPRSWPGSYAVQ VPQPPSQGRTPTVHLAPKPG ALTPPGGPWPQRTERVESAA WGH in AAA16703 (PubMed:8134117).CuratedAdd BLAST42

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_041679354A → T in an ovarian mucinous carcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_041680496A → T1 PublicationCorresponds to variant dbSNP:rs35351680Ensembl.1
Natural variantiVAR_041681503R → Q in a colorectal adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs778726488Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0442771 – 41Missing in isoform 3. 1 PublicationAdd BLAST41
Alternative sequenceiVSP_0431231 – 24MAGRG…EELPR → MQGHFPAERREGRPRRGTRG QQQLL in isoform 2. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L18974 mRNA Translation: AAA16703.1
X77278 mRNA Translation: CAA54493.1
S75164
, S75145, S75147, S75166, S75168, S75151, S75153, S75155, S75156, S75158, S75159, S75162 Genomic DNA Translation: AAC60645.1
AK055395 mRNA Translation: BAG51511.1
AL137754 mRNA Translation: CAB70906.2
AC005777 Genomic DNA Translation: AAC62843.1
CH471139 Genomic DNA Translation: EAW69285.1
BC000114 mRNA Translation: AAH00114.1
BC003109 mRNA Translation: AAH03109.1
CCDSiCCDS12113.1 [P42679-2]
CCDS12114.1 [P42679-1]
CCDS42468.1 [P42679-3]
PIRiA49865
A55625
RefSeqiNP_002369.2, NM_002378.3 [P42679-2]
NP_647611.1, NM_139354.2 [P42679-3]
NP_647612.1, NM_139355.2 [P42679-1]
XP_011526320.1, XM_011528018.1 [P42679-1]
UniGeneiHs.631845

Genome annotation databases

EnsembliENST00000310132; ENSP00000308734; ENSG00000007264 [P42679-1]
ENST00000395040; ENSP00000378481; ENSG00000007264 [P42679-3]
ENST00000395045; ENSP00000378485; ENSG00000007264 [P42679-2]
ENST00000619596; ENSP00000483213; ENSG00000007264 [P42679-2]
GeneIDi4145
KEGGihsa:4145
UCSCiuc002lyt.4 human [P42679-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMATK_HUMAN
AccessioniPrimary (citable) accession number: P42679
Secondary accession number(s): B3KNZ9, Q9NST8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 28, 2018
This is version 187 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health