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Protein

Neurolysin, mitochondrial

Gene

Nln

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.

Catalytic activityi

Preferential cleavage in neurotensin: 10-Pro-|-Tyr-11.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi497Zinc; catalyticPROSITE-ProRule annotation1
Active sitei498PROSITE-ProRule annotation1
Metal bindingi501Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi504Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: RGD
  • oligopeptidase activity Source: RGD
  • peptidase activity Source: RGD
  • peptide binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM03.002

Names & Taxonomyi

Protein namesi
Recommended name:
Neurolysin, mitochondrial (EC:3.4.24.16)
Alternative name(s):
Microsomal endopeptidase
Short name:
MEP
Mitochondrial oligopeptidase M
Neurotensin endopeptidase
Gene namesi
Name:Nln
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621518 Nln

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37Mitochondrion1 PublicationAdd BLAST37
ChainiPRO_000002857838 – 704Neurolysin, mitochondrialAdd BLAST667

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei664N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP42676
PRIDEiP42676

PTM databases

iPTMnetiP42676
PhosphoSitePlusiP42676
SwissPalmiP42676

Miscellaneous databases

PMAP-CutDBiP42676

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000059652

Structurei

Secondary structure

1704
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni43 – 45Combined sources3
Helixi54 – 75Combined sources22
Helixi80 – 82Combined sources3
Turni85 – 88Combined sources4
Helixi89 – 107Combined sources19
Helixi109 – 112Combined sources4
Helixi116 – 137Combined sources22
Helixi140 – 152Combined sources13
Helixi160 – 175Combined sources16
Turni176 – 179Combined sources4
Helixi182 – 187Combined sources6
Helixi189 – 208Combined sources20
Beta strandi212 – 216Combined sources5
Turni217 – 222Combined sources6
Helixi225 – 229Combined sources5
Beta strandi231 – 233Combined sources3
Beta strandi235 – 244Combined sources10
Helixi245 – 254Combined sources10
Helixi258 – 268Combined sources11
Turni269 – 272Combined sources4
Helixi273 – 293Combined sources21
Helixi299 – 304Combined sources6
Helixi312 – 347Combined sources36
Helixi358 – 360Combined sources3
Helixi361 – 372Combined sources12
Helixi377 – 380Combined sources4
Helixi381 – 383Combined sources3
Helixi386 – 401Combined sources16
Beta strandi403 – 407Combined sources5
Beta strandi419 – 425Combined sources7
Turni426 – 428Combined sources3
Beta strandi431 – 438Combined sources8
Beta strandi450 – 455Combined sources6
Beta strandi468 – 473Combined sources6
Beta strandi481 – 483Combined sources3
Helixi489 – 507Combined sources19
Beta strandi510 – 512Combined sources3
Helixi513 – 515Combined sources3
Turni522 – 526Combined sources5
Helixi527 – 533Combined sources7
Helixi534 – 537Combined sources4
Helixi539 – 545Combined sources7
Beta strandi549 – 551Combined sources3
Helixi557 – 565Combined sources9
Turni566 – 570Combined sources5
Helixi571 – 588Combined sources18
Helixi596 – 606Combined sources11
Helixi618 – 621Combined sources4
Beta strandi625 – 628Combined sources4
Helixi635 – 649Combined sources15
Helixi651 – 654Combined sources4
Helixi659 – 669Combined sources11
Turni670 – 672Combined sources3
Helixi673 – 675Combined sources3
Helixi678 – 686Combined sources9
Helixi693 – 699Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I1IX-ray2.30P24-704[»]
2O3EX-ray2.20A24-701[»]
4FXYX-ray2.80P/Q24-704[»]
ProteinModelPortaliP42676
SMRiP42676
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42676

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2089 Eukaryota
COG0339 LUCA
HOGENOMiHOG000245985
HOVERGENiHBG000238
InParanoidiP42676
KOiK01393
PhylomeDBiP42676
TreeFamiTF300459

Family and domain databases

Gene3Di1.10.1370.10, 2 hits
1.20.1050.40, 1 hit
3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR024079 MetalloPept_cat_dom_sf
IPR024077 Neurolysin/TOP_dom2
IPR024080 Neurolysin/TOP_N
IPR001567 Pept_M3A_M3B
PfamiView protein in Pfam
PF01432 Peptidase_M3, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITLCLSTLR GLHRAGGSRL QLTMTLGKEL ASPLQAMSSY TAAGRNVLRW
60 70 80 90 100
DLSPEQIKTR TEQLIAQTKQ VYDTVGTIAL KEVTYENCLQ VLADIEVTYI
110 120 130 140 150
VERTMLDFPQ HVSSDREVRA ASTEADKKLS RFDIEMSMRE DVFQRIVHLQ
160 170 180 190 200
ETCDLEKIKP EARRYLEKSI KMGKRNGLHL SEHIRNEIKS MKKRMSELCI
210 220 230 240 250
DFNKNLNEDD TSLVFSKAEL GALPDDFIDS LEKTDEDKYK VTLKYPHYFP
260 270 280 290 300
VMKKCCVPET RRKMEMAFHT RCKQENTAIL QQLLPLRAQV AKLLGYNTHA
310 320 330 340 350
DFVLELNTAK STSRVAAFLD DLSQKLKPLG EAEREFILSL KKKECEERGF
360 370 380 390 400
EYDGKINAWD LHYYMTQTEE LKYSVDQESL KEYFPIEVVT EGLLSIYQEL
410 420 430 440 450
LGLSFEQVPD AHVWNKSVSL YTVKDKATGE VLGQFYLDLY PREGKYNHAA
460 470 480 490 500
CFGLQPGCLL PDGSRMMSVA ALVVNFSQPV AGRPSLLRHD EVRTYFHEFG
510 520 530 540 550
HVMHQICAQT DFARFSGTNV ETDFVEVPSQ MLENWVWDVD SLRKLSKHYK
560 570 580 590 600
DGHPITDELL EKLVASRLVN TGLLTLRQIV LSKVDQSLHT NATLDAASEY
610 620 630 640 650
AKYCTEILGV AATPGTNMPA TFGHLAGGYD GQYYGYLWSE VFSMDMFHSC
660 670 680 690 700
FKKEGIMNPE VGMKYRNLIL KPGGSLDGMD MLQNFLQREP NQKAFLMSRG

LNGS
Length:704
Mass (Da):80,254
Last modified:November 1, 1995 - v1
Checksum:iE33F7967A79343D1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti128K → R in AAH72687 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87157 mRNA Translation: CAA60630.1
BC072687 mRNA Translation: AAH72687.1
RefSeqiNP_446422.2, NM_053970.2
UniGeneiRn.11029

Genome annotation databases

GeneIDi117041
KEGGirno:117041
UCSCiRGD:621518 rat

Similar proteinsi

Entry informationi

Entry nameiNEUL_RAT
AccessioniPrimary (citable) accession number: P42676
Secondary accession number(s): Q6GQQ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health