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Protein

Transcriptional activator protein Pur-alpha

Gene

Pura

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the c-Myc gene. May play a role in the initiation of DNA replication and in recombination.

GO - Molecular functioni

  • DNA binding Source: MGI
  • double-stranded DNA binding Source: MGI
  • double-stranded telomeric DNA binding Source: MGI
  • poly(A) RNA binding Source: MGI
  • purine-rich negative regulatory element binding Source: HGNC
  • sequence-specific DNA binding transcription factor activity Source: MGI
  • single-stranded DNA binding Source: UniProtKB
  • SMAD binding Source: MGI
  • transcription factor binding Source: UniProtKB
  • translation repressor activity, nucleic acid binding Source: HGNC

GO - Biological processi

  • apoptotic process Source: UniProtKB
  • cell differentiation Source: UniProtKB
  • cell proliferation Source: UniProtKB
  • DNA unwinding involved in DNA replication Source: MGI
  • mitotic cell cycle checkpoint Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: HGNC
  • negative regulation of translation Source: GOC
  • nervous system development Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator protein Pur-alpha
Alternative name(s):
Purine-rich single-stranded DNA-binding protein alpha
Gene namesi
Name:Pura
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:103079. Pura.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • dendrite Source: MGI
  • DNA replication factor A complex Source: UniProtKB
  • neuronal cell body Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 321320Transcriptional activator protein Pur-alphaPRO_0000097108Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP42669.
PaxDbiP42669.
PRIDEiP42669.

2D gel databases

UCD-2DPAGEP42669.

PTM databases

PhosphoSiteiP42669.

Expressioni

Gene expression databases

BgeeiP42669.
CleanExiMM_PURA.
GenevisibleiP42669. MM.

Interactioni

Subunit structurei

Homodimer, heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1.1 Publication

Protein-protein interaction databases

BioGridi202515. 14 interactions.
IntActiP42669. 5 interactions.
MINTiMINT-243159.
STRINGi10090.ENSMUSP00000059404.

Structurei

3D structure databases

ProteinModelPortaliP42669.
SMRiP42669. Positions 58-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 5242Gly-richAdd
BLAST
Compositional biasi292 – 32130Gln/Glu-rich (part of the transcriptional activation domain)Add
BLAST

Sequence similaritiesi

Belongs to the PUR DNA-binding protein family.Curated

Phylogenomic databases

eggNOGiNOG312871.
GeneTreeiENSGT00390000015406.
HOGENOMiHOG000232132.
HOVERGENiHBG006888.
InParanoidiP42669.
OMAiVPYKVWS.
OrthoDBiEOG78D7M4.
PhylomeDBiP42669.
TreeFamiTF313701.

Family and domain databases

InterProiIPR006628. PUR-bd_fam.
IPR030500. PURalpha.
[Graphical view]
PANTHERiPTHR12611. PTHR12611. 1 hit.
PTHR12611:SF2. PTHR12611:SF2. 1 hit.
PfamiPF04845. PurA. 1 hit.
[Graphical view]
SMARTiSM00712. PUR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42669-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRDSGSEQ GGAALGSGGS LGHPGSGSGS GGGGGGGGGG GGSGGGGGAP
60 70 80 90 100
GGLQHETQEL ASKRVDIQNK RFYLDVKQNA KGRFLKIAEV GAGGNKSRLT
110 120 130 140 150
LSMSVAVEFR DYLGDFIEHY AQLGPSQPPD LAQAQDEPRR ALKSEFLVRE
160 170 180 190 200
NRKYYMDLKE NQRGRFLRIR QTVNRGPGLG STQGQTIALP AQGLIEFRDA
210 220 230 240 250
LAKLIDDYGV EEEPAELPEG TSLTVDNKRF FFDVGSNKYG VFMRVSEVKP
260 270 280 290 300
TYRNSITVPY KVWAKFGHTF CKYSEEMKKI QEKQREKRAA CEQLHQQQQQ
310 320
QQEETTAATL LLQGEEEGEE D
Length:321
Mass (Da):34,884
Last modified:November 1, 1995 - v1
Checksum:i0379DBD96D47DCEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02098 mRNA. Translation: AAA64630.1.
AF017631 mRNA. Translation: AAB71860.1.
CCDSiCCDS29151.1.
RefSeqiNP_033015.1. NM_008989.3.
XP_006525787.1. XM_006525724.2.
XP_011245162.1. XM_011246860.1.
UniGeneiMm.231802.
Mm.482241.

Genome annotation databases

EnsembliENSMUST00000051301; ENSMUSP00000059404; ENSMUSG00000043991.
GeneIDi19290.
KEGGimmu:19290.
UCSCiuc008enh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02098 mRNA. Translation: AAA64630.1.
AF017631 mRNA. Translation: AAB71860.1.
CCDSiCCDS29151.1.
RefSeqiNP_033015.1. NM_008989.3.
XP_006525787.1. XM_006525724.2.
XP_011245162.1. XM_011246860.1.
UniGeneiMm.231802.
Mm.482241.

3D structure databases

ProteinModelPortaliP42669.
SMRiP42669. Positions 58-276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202515. 14 interactions.
IntActiP42669. 5 interactions.
MINTiMINT-243159.
STRINGi10090.ENSMUSP00000059404.

PTM databases

PhosphoSiteiP42669.

2D gel databases

UCD-2DPAGEP42669.

Proteomic databases

MaxQBiP42669.
PaxDbiP42669.
PRIDEiP42669.

Protocols and materials databases

DNASUi19290.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051301; ENSMUSP00000059404; ENSMUSG00000043991.
GeneIDi19290.
KEGGimmu:19290.
UCSCiuc008enh.2. mouse.

Organism-specific databases

CTDi5813.
MGIiMGI:103079. Pura.

Phylogenomic databases

eggNOGiNOG312871.
GeneTreeiENSGT00390000015406.
HOGENOMiHOG000232132.
HOVERGENiHBG006888.
InParanoidiP42669.
OMAiVPYKVWS.
OrthoDBiEOG78D7M4.
PhylomeDBiP42669.
TreeFamiTF313701.

Miscellaneous databases

NextBioi296222.
PROiP42669.
SOURCEiSearch...

Gene expression databases

BgeeiP42669.
CleanExiMM_PURA.
GenevisibleiP42669. MM.

Family and domain databases

InterProiIPR006628. PUR-bd_fam.
IPR030500. PURalpha.
[Graphical view]
PANTHERiPTHR12611. PTHR12611. 1 hit.
PTHR12611:SF2. PTHR12611:SF2. 1 hit.
PfamiPF04845. PurA. 1 hit.
[Graphical view]
SMARTiSM00712. PUR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Conservation in human and mouse Pur alpha of a motif common to several proteins involved in initiation of DNA replication."
    Ma Z.-W., Bergemann A.D., Johnson E.M.
    Gene 149:311-314(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence of cDNAs encoding components of vascular actin single-stranded DNA-binding factor 2 establish identity to Puralpha and Purbeta."
    Kelm R.J. Jr., Elder P.K., Strauch A.R., Getz M.J.
    J. Biol. Chem. 272:26727-26733(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Lung.
  3. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 176-198 AND 204-228, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  4. "Molecular interactions between single-stranded DNA-binding proteins associated with an essential MCAT element in the mouse smooth muscle alpha-actin promoter."
    Kelm R.J. Jr., Cogan J.G., Elder P.K., Strauch A.R., Getz M.J.
    J. Biol. Chem. 274:14238-14245(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  5. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPURA_MOUSE
AccessioniPrimary (citable) accession number: P42669
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 22, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.