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P42669

- PURA_MOUSE

UniProt

P42669 - PURA_MOUSE

Protein

Transcriptional activator protein Pur-alpha

Gene

Pura

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the c-Myc gene. May play a role in the initiation of DNA replication and in recombination.

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. double-stranded DNA binding Source: MGI
    3. double-stranded telomeric DNA binding Source: Ensembl
    4. protein binding Source: MGI
    5. purine-rich negative regulatory element binding Source: HGNC
    6. sequence-specific DNA binding transcription factor activity Source: MGI
    7. single-stranded DNA binding Source: UniProtKB
    8. SMAD binding Source: MGI
    9. transcription factor binding Source: UniProtKB
    10. translation repressor activity, nucleic acid binding Source: HGNC

    GO - Biological processi

    1. apoptotic process Source: UniProtKB
    2. cell differentiation Source: UniProtKB
    3. cell proliferation Source: UniProtKB
    4. DNA unwinding involved in DNA replication Source: Ensembl
    5. mitotic cell cycle checkpoint Source: UniProtKB
    6. negative regulation of transcription, DNA-templated Source: HGNC
    7. negative regulation of translation Source: GOC
    8. nervous system development Source: MGI
    9. positive regulation of cell proliferation Source: MGI
    10. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional activator protein Pur-alpha
    Alternative name(s):
    Purine-rich single-stranded DNA-binding protein alpha
    Gene namesi
    Name:Pura
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:103079. Pura.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. dendrite Source: MGI
    3. DNA replication factor A complex Source: UniProtKB
    4. neuronal cell body Source: MGI
    5. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 321321Transcriptional activator protein Pur-alphaPRO_0000097108Add
    BLAST

    Proteomic databases

    MaxQBiP42669.
    PaxDbiP42669.
    PRIDEiP42669.

    2D gel databases

    UCD-2DPAGEP42669.

    PTM databases

    PhosphoSiteiP42669.

    Expressioni

    Gene expression databases

    BgeeiP42669.
    CleanExiMM_PURA.
    GenevestigatoriP42669.

    Interactioni

    Subunit structurei

    Homodimer, heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1.1 Publication

    Protein-protein interaction databases

    BioGridi202515. 14 interactions.
    IntActiP42669. 5 interactions.
    MINTiMINT-243159.
    STRINGi10090.ENSMUSP00000059404.

    Structurei

    3D structure databases

    ProteinModelPortaliP42669.
    SMRiP42669. Positions 58-276.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi11 – 5242Gly-richAdd
    BLAST
    Compositional biasi292 – 32130Gln/Glu-rich (part of the transcriptional activation domain)Add
    BLAST

    Sequence similaritiesi

    Belongs to the PUR DNA-binding protein family.Curated

    Phylogenomic databases

    eggNOGiNOG312871.
    GeneTreeiENSGT00390000015406.
    HOGENOMiHOG000232132.
    HOVERGENiHBG006888.
    InParanoidiP42669.
    OMAiREKRACE.
    OrthoDBiEOG78D7M4.
    PhylomeDBiP42669.
    TreeFamiTF313701.

    Family and domain databases

    InterProiIPR006628. PUR_DNA_RNA-bd.
    [Graphical view]
    PANTHERiPTHR12611. PTHR12611. 1 hit.
    PfamiPF04845. PurA. 1 hit.
    [Graphical view]
    SMARTiSM00712. PUR. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42669-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADRDSGSEQ GGAALGSGGS LGHPGSGSGS GGGGGGGGGG GGSGGGGGAP    50
    GGLQHETQEL ASKRVDIQNK RFYLDVKQNA KGRFLKIAEV GAGGNKSRLT 100
    LSMSVAVEFR DYLGDFIEHY AQLGPSQPPD LAQAQDEPRR ALKSEFLVRE 150
    NRKYYMDLKE NQRGRFLRIR QTVNRGPGLG STQGQTIALP AQGLIEFRDA 200
    LAKLIDDYGV EEEPAELPEG TSLTVDNKRF FFDVGSNKYG VFMRVSEVKP 250
    TYRNSITVPY KVWAKFGHTF CKYSEEMKKI QEKQREKRAA CEQLHQQQQQ 300
    QQEETTAATL LLQGEEEGEE D 321
    Length:321
    Mass (Da):34,884
    Last modified:November 1, 1995 - v1
    Checksum:i0379DBD96D47DCEA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02098 mRNA. Translation: AAA64630.1.
    AF017631 mRNA. Translation: AAB71860.1.
    CCDSiCCDS29151.1.
    RefSeqiNP_033015.1. NM_008989.3.
    XP_006525787.1. XM_006525724.1.
    UniGeneiMm.231802.
    Mm.482241.

    Genome annotation databases

    EnsembliENSMUST00000051301; ENSMUSP00000059404; ENSMUSG00000043991.
    GeneIDi19290.
    KEGGimmu:19290.
    UCSCiuc008enh.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02098 mRNA. Translation: AAA64630.1 .
    AF017631 mRNA. Translation: AAB71860.1 .
    CCDSi CCDS29151.1.
    RefSeqi NP_033015.1. NM_008989.3.
    XP_006525787.1. XM_006525724.1.
    UniGenei Mm.231802.
    Mm.482241.

    3D structure databases

    ProteinModelPortali P42669.
    SMRi P42669. Positions 58-276.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202515. 14 interactions.
    IntActi P42669. 5 interactions.
    MINTi MINT-243159.
    STRINGi 10090.ENSMUSP00000059404.

    PTM databases

    PhosphoSitei P42669.

    2D gel databases

    UCD-2DPAGE P42669.

    Proteomic databases

    MaxQBi P42669.
    PaxDbi P42669.
    PRIDEi P42669.

    Protocols and materials databases

    DNASUi 19290.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000051301 ; ENSMUSP00000059404 ; ENSMUSG00000043991 .
    GeneIDi 19290.
    KEGGi mmu:19290.
    UCSCi uc008enh.2. mouse.

    Organism-specific databases

    CTDi 5813.
    MGIi MGI:103079. Pura.

    Phylogenomic databases

    eggNOGi NOG312871.
    GeneTreei ENSGT00390000015406.
    HOGENOMi HOG000232132.
    HOVERGENi HBG006888.
    InParanoidi P42669.
    OMAi REKRACE.
    OrthoDBi EOG78D7M4.
    PhylomeDBi P42669.
    TreeFami TF313701.

    Miscellaneous databases

    NextBioi 296222.
    PROi P42669.
    SOURCEi Search...

    Gene expression databases

    Bgeei P42669.
    CleanExi MM_PURA.
    Genevestigatori P42669.

    Family and domain databases

    InterProi IPR006628. PUR_DNA_RNA-bd.
    [Graphical view ]
    PANTHERi PTHR12611. PTHR12611. 1 hit.
    Pfami PF04845. PurA. 1 hit.
    [Graphical view ]
    SMARTi SM00712. PUR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Conservation in human and mouse Pur alpha of a motif common to several proteins involved in initiation of DNA replication."
      Ma Z.-W., Bergemann A.D., Johnson E.M.
      Gene 149:311-314(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Sequence of cDNAs encoding components of vascular actin single-stranded DNA-binding factor 2 establish identity to Puralpha and Purbeta."
      Kelm R.J. Jr., Elder P.K., Strauch A.R., Getz M.J.
      J. Biol. Chem. 272:26727-26733(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X CBA.
      Tissue: Lung.
    3. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 176-198 AND 204-228, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    4. "Molecular interactions between single-stranded DNA-binding proteins associated with an essential MCAT element in the mouse smooth muscle alpha-actin promoter."
      Kelm R.J. Jr., Cogan J.G., Elder P.K., Strauch A.R., Getz M.J.
      J. Biol. Chem. 274:14238-14245(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.

    Entry informationi

    Entry nameiPURA_MOUSE
    AccessioniPrimary (citable) accession number: P42669
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3