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Protein

DNA damage checkpoint protein rad24

Gene

rad24

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the DNA damage checkpoint that ensures that DNA damage is repaired before mitosis is attempted. Acts as a negative regulator of meiosis by antagonizing the function of mei2. It inhibits the association of meiRNA (a non-coding RNA molecule required for the nuclear mei2 dot formation) to the phosphorylated but not to the unphosphorylated form of mei2 in vitro.1 Publication

GO - Molecular functioni

  • protein domain specific binding Source: InterPro
  • protein phosphatase inhibitor activity Source: PomBase

GO - Biological processi

  • cellular protein localization Source: PomBase
  • cytoplasmic sequestering of protein Source: PomBase
  • cytoplasmic sequestering of protein involved in mitotic DNA replication checkpoint Source: PomBase
  • cytoplasmic sequestering of protein involved in mitotic G2 DNA damage checkpoint Source: PomBase
  • meiotic cell cycle Source: UniProtKB-KW
  • mitotic cytokinesis checkpoint Source: PomBase
  • mitotic G2 DNA damage checkpoint Source: PomBase
  • negative regulation of DNA binding transcription factor activity Source: PomBase
  • negative regulation of induction of conjugation with cellular fusion Source: PomBase
  • negative regulation of protein export from nucleus Source: PomBase
  • negative regulation of transcription by RNA polymerase II Source: PomBase
  • positive regulation of septation initiation signaling Source: PomBase

Keywordsi

Biological processCell cycle, DNA damage, Meiosis

Enzyme and pathway databases

ReactomeiR-SPO-3371453 Regulation of HSF1-mediated heat shock response
R-SPO-3371511 HSF1 activation
R-SPO-5625740 RHO GTPases activate PKNs
R-SPO-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Names & Taxonomyi

Protein namesi
Recommended name:
DNA damage checkpoint protein rad24
Gene namesi
Name:rad24
ORF Names:SPAC8E11.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC8E11.02c
PomBaseiSPAC8E11.02c rad24

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000587171 – 270DNA damage checkpoint protein rad24Add BLAST270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34Phosphoserine1 Publication1
Modified residuei66Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP42656
PaxDbiP42656
PRIDEiP42656

PTM databases

iPTMnetiP42656
SwissPalmiP42656

Interactioni

Subunit structurei

Binds preferentially to mei2 phosphorylated by ran1/pat1.

Binary interactionsi

WithEntry#Exp.IntActNotes
clp1Q9P7H13EBI-704791,EBI-704737

GO - Molecular functioni

Protein-protein interaction databases

BioGridi278511, 81 interactors
ELMiP42656
IntActiP42656, 3 interactors
STRINGi4896.SPAC8E11.02c.1

Structurei

3D structure databases

ProteinModelPortaliP42656
SMRiP42656
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

HOGENOMiHOG000240379
InParanoidiP42656
KOiK06630
OMAiIPCATTG
OrthoDBiEOG092C46UY
PhylomeDBiP42656

Family and domain databases

Gene3Di1.20.190.20, 1 hit
InterProiView protein in InterPro
IPR000308 14-3-3
IPR023409 14-3-3_CS
IPR036815 14-3-3_dom_sf
IPR023410 14-3-3_domain
PANTHERiPTHR18860 PTHR18860, 1 hit
PfamiView protein in Pfam
PF00244 14-3-3, 1 hit
PIRSFiPIRSF000868 14-3-3, 1 hit
PRINTSiPR00305 1433ZETA
SMARTiView protein in SMART
SM00101 14_3_3, 1 hit
SUPFAMiSSF48445 SSF48445, 1 hit
PROSITEiView protein in PROSITE
PS00796 1433_1, 1 hit
PS00797 1433_2, 1 hit

Sequencei

Sequence statusi: Complete.

P42656-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTSREDAV YLAKLAEQAE RYEGMVENMK SVASTDQELT VEERNLLSVA
60 70 80 90 100
YKNVIGARRA SWRIVSSIEQ KEESKGNTAQ VELIKEYRQK IEQELDTICQ
110 120 130 140 150
DILTVLEKHL IPNAASAESK VFYYKMKGDY YRYLAEFAVG EKRQHSADQS
160 170 180 190 200
LEGYKAASEI ATAELAPTHP IRLGLALNFS VFYYEILNSP DRACYLAKQA
210 220 230 240 250
FDEAISELDS LSEESYKDST LIMQLLRDNL TLWTSDAEYS AAAAGGNTEG
260 270
AQENAPSNAP EGEAEPKADA
Length:270
Mass (Da):30,082
Last modified:July 15, 1998 - v2
Checksum:i1401DD425A1A872F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti219S → F in BAA24800 (PubMed:12242289).Curated1
Sequence conflicti264A → R in CAA55795 (PubMed:8036497).Curated1
Sequence conflicti269 – 270DA → THR in CAA55795 (PubMed:8036497).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79206 Genomic DNA Translation: CAA55795.1
AB010899 mRNA Translation: BAA24800.1
AB008545 Genomic DNA Translation: BAA28672.1
CU329670 Genomic DNA Translation: CAA17023.1
PIRiT39156
T43316
T45211
RefSeqiNP_594167.1, NM_001019591.2

Genome annotation databases

EnsemblFungiiSPAC8E11.02c.1; SPAC8E11.02c.1:pep; SPAC8E11.02c
GeneIDi2542029
KEGGispo:SPAC8E11.02c

Similar proteinsi

Entry informationi

Entry nameiRAD24_SCHPO
AccessioniPrimary (citable) accession number: P42656
Secondary accession number(s): O42704, O42879
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 15, 1998
Last modified: May 23, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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