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Protein

14-3-3-like protein GF14 psi

Gene

GRF3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes (PubMed:7972511, PubMed:7870824, PubMed:16407442). Involved in the regulation of nutrient metabolism (PubMed:22104211, PubMed:21094157). Reciprocal negative transcription regulation of miR396 (PubMed:22751317). Negative regulator of constitutive freezing tolerance and cold acclimation by controlling cold-induced gene expression partially through an ethylene (ET)-dependent pathway; prevents ethylene (ET) biosynthesis, probably by binding 1-aminocyclopropane-1-carboxylate synthases (ACS) to reduce their stability, thus contributing to establish adequate ET levels under both standard and low-temperature conditions (PubMed:25122152).2 Publications5 Publications

GO - Molecular functioni

  • ATP binding Source: TAIR

GO - Biological processi

  • cellular response to nitrogen starvation Source: UniProtKB
  • cellular response to phosphate starvation Source: UniProtKB
  • cellular response to potassium ion starvation Source: UniProtKB
  • cold acclimation Source: UniProtKB
  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • regulation of metabolic process Source: UniProtKB
  • response to cold Source: UniProtKB
  • response to freezing Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ethylene signaling pathway, Stress response

Enzyme and pathway databases

ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371511. HSF1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3-like protein GF14 psi1 Publication
Alternative name(s):
General regulatory factor 31 Publication
Protein RARE COLD INDUCIBLE 1A1 Publication
Gene namesi
Name:GRF31 Publication
Synonyms:RCI1A1 Publication
Ordered Locus Names:At5g38480Imported
ORF Names:MXI10.21Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G38480.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • chloroplast Source: TAIR
  • cytoplasm Source: UniProtKB
  • cytosol Source: TAIR
  • Golgi apparatus Source: TAIR
  • mitochondrion Source: TAIR
  • nucleus Source: UniProtKB
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Short primary roots and reduced size, with smaller cells (PubMed:21094157, PubMed:25122152). Disturbed levels of several metabolites (e.g. beta-alanine, threonic acid, phenylalanine, 1,3-diaminopropane dihydrochloride, citrate, glycine, aspartate, glucose, 1,4-diaminobutane, proline, palmitate and shikimate) (PubMed:22104211). Increased miR396 levels in plants lacking simultaneously GRF1, GRF2 and GRF3 (PubMed:22751317). Higher freezing tolerance, and greater levels of cold-inducible genes and ethylene- (ET-) inducible genes leading to higher ET levels, as well as slightly enhanced expression of ACS6 (PubMed:25122152).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586651 – 25514-3-3-like protein GF14 psiAdd BLAST255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162PhosphothreonineCombined sources1
Modified residuei238PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP42644.
PRIDEiP42644.

PTM databases

iPTMnetiP42644.

Expressioni

Inductioni

By cold in an abscisic acid- (ABA-) independent manner (at protein level) (PubMed:7520301, PubMed:25122152). Repressed by phosphate (Pi) deprivation (PubMed:17598127). Induced by nitrogen (N) and phosphate (P) deprivation in leaves, but repressed by potassium (K) and N deprivation in roots (PubMed:21094157). Repressed by miR396 (PubMed:22751317).5 Publications

Gene expression databases

ExpressionAtlasiP42644. baseline and differential.
GenevisibleiP42644. AT.

Interactioni

Subunit structurei

Component of a DNA binding complex that binds to the G box (PubMed:16407442). Interacts with IDH3, AGT3, GLN1-1, GLN1-2, GLN1-4, SAM1, SAM2, MDH1, METK3 and MDH2 (PubMed:22104211, PubMed:21094157). Binds to 1-aminocyclopropane-1-carboxylate synthases (ACS) such as ACS2, ACS5, ACS6, ACS8, and ACS11 (PubMed:25122152). Interacts with FD (PubMed:25661797).5 Publications

Protein-protein interaction databases

BioGridi19087. 229 interactors.
IntActiP42644. 9 interactors.
MINTiMINT-8067157.
STRINGi3702.AT5G38480.1.

Structurei

3D structure databases

ProteinModelPortaliP42644.
SMRiP42644.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
HOGENOMiHOG000240379.
KOiK06630.
OMAiMSTREEN.
OrthoDBiEOG09360IZO.
PhylomeDBiP42644.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P42644-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTREENVYM AKLAEQAERY EEMVEFMEKV AKTVDVEELS VEERNLLSVA
60 70 80 90 100
YKNVIGARRA SWRIISSIEQ KEESKGNEDH VAIIKDYRGK IESELSKICD
110 120 130 140 150
GILNVLEAHL IPSASPAESK VFYLKMKGDY HRYLAEFKAG AERKEAAEST
160 170 180 190 200
LVAYKSASDI ATAELAPTHP IRLGLALNFS VFYYEILNSP DRACSLAKQA
210 220 230 240 250
FDDAIAELDT LGEESYKDST LIMQLLRDNL TLWTSDMTDE AGDEIKEASK

PDGAE
Length:255
Mass (Da):28,606
Last modified:December 12, 2006 - v2
Checksum:i51E018F146ED2A0F
GO
Isoform 2 (identifier: P42644-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     239-241: DEA → VT

Note: Derived from EST data. No experimental confirmation available.Imported
Show »
Length:254
Mass (Da):28,491
Checksum:iBE89D6B67FA41C4C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90K → E in AAA32799 (PubMed:7972511).Curated1
Sequence conflicti90K → E in AAA96252 (PubMed:7870824).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057062239 – 241DEA → VT in isoform 2. Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09110 mRNA. Translation: AAA32799.1.
X74140 mRNA. Translation: CAA52237.1.
U09375 Genomic DNA. Translation: AAA96252.1.
AB005231, AB005248 Genomic DNA. Translation: BAB10138.1.
CP002688 Genomic DNA. Translation: AED94323.1.
CP002688 Genomic DNA. Translation: AED94324.1.
AF412093 mRNA. Translation: AAL06546.1.
AY093976 mRNA. Translation: AAM16237.1.
AK226217 mRNA. Translation: BAE98382.1.
AK228478 mRNA. Translation: BAF00404.1.
PIRiS47969.
S57277.
RefSeqiNP_568557.1. NM_123209.4. [P42644-1]
UniGeneiAt.22344.
At.22949.

Genome annotation databases

EnsemblPlantsiAT5G38480.1; AT5G38480.1; AT5G38480. [P42644-1]
AT5G38480.2; AT5G38480.2; AT5G38480. [P42644-2]
GeneIDi833836.
GrameneiAT5G38480.1; AT5G38480.1; AT5G38480.
AT5G38480.2; AT5G38480.2; AT5G38480.
KEGGiath:AT5G38480.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09110 mRNA. Translation: AAA32799.1.
X74140 mRNA. Translation: CAA52237.1.
U09375 Genomic DNA. Translation: AAA96252.1.
AB005231, AB005248 Genomic DNA. Translation: BAB10138.1.
CP002688 Genomic DNA. Translation: AED94323.1.
CP002688 Genomic DNA. Translation: AED94324.1.
AF412093 mRNA. Translation: AAL06546.1.
AY093976 mRNA. Translation: AAM16237.1.
AK226217 mRNA. Translation: BAE98382.1.
AK228478 mRNA. Translation: BAF00404.1.
PIRiS47969.
S57277.
RefSeqiNP_568557.1. NM_123209.4. [P42644-1]
UniGeneiAt.22344.
At.22949.

3D structure databases

ProteinModelPortaliP42644.
SMRiP42644.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19087. 229 interactors.
IntActiP42644. 9 interactors.
MINTiMINT-8067157.
STRINGi3702.AT5G38480.1.

PTM databases

iPTMnetiP42644.

Proteomic databases

PaxDbiP42644.
PRIDEiP42644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G38480.1; AT5G38480.1; AT5G38480. [P42644-1]
AT5G38480.2; AT5G38480.2; AT5G38480. [P42644-2]
GeneIDi833836.
GrameneiAT5G38480.1; AT5G38480.1; AT5G38480.
AT5G38480.2; AT5G38480.2; AT5G38480.
KEGGiath:AT5G38480.

Organism-specific databases

TAIRiAT5G38480.

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
HOGENOMiHOG000240379.
KOiK06630.
OMAiMSTREEN.
OrthoDBiEOG09360IZO.
PhylomeDBiP42644.

Enzyme and pathway databases

ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371511. HSF1 activation.

Miscellaneous databases

PROiP42644.

Gene expression databases

ExpressionAtlasiP42644. baseline and differential.
GenevisibleiP42644. AT.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei14333_ARATH
AccessioniPrimary (citable) accession number: P42644
Secondary accession number(s): F4KBI7, Q945L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 12, 2006
Last modified: November 30, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.