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Protein

14-3-3-like protein GF14 chi

Gene

GRF1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes. Involved in the regulation of nutrient metabolism (PubMed:22104211).1 Publication

GO - Molecular functioni

GO - Biological processi

  • regulation of metabolic process Source: UniProtKB

Enzyme and pathway databases

ReactomeiR-ATH-1445148. Translocation of GLUT4 to the plasma membrane.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371511. HSF1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3-like protein GF14 chi
Alternative name(s):
General regulatory factor 1
Gene namesi
Name:GRF1
Ordered Locus Names:At4g09000
ORF Names:F23J3.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G09000.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Disturbed levels of several metabolites (e.g. beta-alanine, aspartate, pyroglutamate, glutamate, glutamine, alpha-ketoglutarate, palmitate and shikimate).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000586632 – 26714-3-3-like protein GF14 chiAdd BLAST266

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei72PhosphoserineBy similarity1
Modified residuei195PhosphoserineBy similarity1
Modified residuei216PhosphothreonineBy similarity1
Modified residuei267PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP42643.

PTM databases

iPTMnetiP42643.

Expressioni

Gene expression databases

ExpressionAtlasiP42643. baseline and differential.
GenevisibleiP42643. AT.

Interactioni

Subunit structurei

Interacts with TPK1 (PubMed:17764516). Interacts with the isocitrate dehydrogenase IDH3, and malate dehydrogenases MDH1 and MDH2 (PubMed:22104211). Interacts with DREB1A and DREB1B in the nucleus (PubMed:28344081).3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi11778. 218 interactors.
IntActiP42643. 6 interactors.
MINTiMINT-8058797.

Structurei

3D structure databases

ProteinModelPortaliP42643.
SMRiP42643.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

HOGENOMiHOG000240379.
KOiK06630.
PhylomeDBiP42643.

Family and domain databases

Gene3Di1.20.190.20. 1 hit.
InterProiView protein in InterPro
IPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR036815. 14-3-3_dom_sf.
IPR023410. 14-3-3_domain.
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiView protein in Pfam
PF00244. 14-3-3. 1 hit.
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiView protein in SMART
SM00101. 14_3_3. 1 hit.
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiView protein in PROSITE
PS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P42643-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATPGASSAR DEFVYMAKLA EQAERYEEMV EFMEKVAKAV DKDELTVEER
60 70 80 90 100
NLLSVAYKNV IGARRASWRI ISSIEQKEES RGNDDHVSLI RDYRSKIETE
110 120 130 140 150
LSDICDGILK LLDTILVPAA ASGDSKVFYL KMKGDYHRYL AEFKSGQERK
160 170 180 190 200
DAAEHTLTAY KAAQDIANSE LAPTHPIRLG LALNFSVFYY EILNSPDRAC
210 220 230 240 250
NLAKQAFDEA IAELDTLGEE SYKDSTLIMQ LLRDNLTLWT SDMQDDVADD
260
IKEAAPAAAK PADEQQS
Length:267
Mass (Da):29,932
Last modified:May 29, 2007 - v3
Checksum:i328FA525D8AE0290
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti240T → A in AAA96323 (PubMed:7972511).Curated1
Sequence conflicti240T → A in AAA96254 (PubMed:7870824).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09112 mRNA. Translation: AAA96323.1.
U09377 Genomic DNA. Translation: AAA96254.1.
AL161513 Genomic DNA. Translation: CAB78024.1.
CP002687 Genomic DNA. Translation: AEE82709.1.
AF412067 mRNA. Translation: AAL06520.1.
AY065064 mRNA. Translation: AAL57697.1.
AY086143 mRNA. Translation: AAM63348.1.
BT024489 mRNA. Translation: ABD19670.1.
PIRiH85090.
S57276.
RefSeqiNP_567344.1. NM_116969.3. [P42643-1]
UniGeneiAt.22637.
At.47549.

Genome annotation databases

EnsemblPlantsiAT4G09000.1; AT4G09000.1; AT4G09000. [P42643-1]
GeneIDi826479.
GrameneiAT4G09000.1; AT4G09000.1; AT4G09000.
KEGGiath:AT4G09000.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry namei14331_ARATH
AccessioniPrimary (citable) accession number: P42643
Secondary accession number(s): Q945M2, Q9M0S7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 29, 2007
Last modified: October 25, 2017
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families