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Protein

Altronate dehydratase

Gene

uxaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dehydration of D-altronate.1 Publication

Catalytic activityi

D-altronate = 2-dehydro-3-deoxy-D-gluconate + H2O.1 Publication

Cofactori

Fe2+1 Publication, Mn2+1 PublicationNote: Mn2+ can substitute for iron, but in higher concentrations. Cannot use Fe3+, Ni2+ or Mg2+.1 Publication

Enzyme regulationi

Is inhibited by high concentrations of Fe2+ (> 2 mM), and by EDTA or other iron chelators in vitro.1 Publication

pH dependencei

Optimum pH is about 7.5. The activity at pH 6.5 and 8.6 is only about 20% of the maximal achievable activity at optimum pH.1 Publication

Pathwayi: pentose and glucuronate interconversion

This protein is involved in the pathway pentose and glucuronate interconversion, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway pentose and glucuronate interconversion and in Carbohydrate metabolism.

GO - Molecular functioni

  • altronate dehydratase activity Source: UniProtKB-EC
  • ferrous iron binding Source: EcoCyc

GO - Biological processi

  • D-galacturonate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Iron, Manganese

Enzyme and pathway databases

BioCyciEcoCyc:ALTRODEHYDRAT-MONOMER.
ECOL316407:JW3062-MONOMER.
MetaCyc:ALTRODEHYDRAT-MONOMER.
UniPathwayiUPA00246.

Names & Taxonomyi

Protein namesi
Recommended name:
Altronate dehydratase (EC:4.2.1.7)
Alternative name(s):
D-altronate hydro-lyase
Gene namesi
Name:uxaA
Synonyms:ygjW
Ordered Locus Names:b3091, JW3062
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12734. uxaA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495Altronate dehydratasePRO_0000172283Add
BLAST

Proteomic databases

PaxDbiP42604.

Expressioni

Inductioni

Repressed by LeuO and H-NS. Part of the uxaCA operon.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262407. 11 interactions.
DIPiDIP-11105N.
IntActiP42604. 6 interactions.
STRINGi511145.b3091.

Structurei

3D structure databases

ProteinModelPortaliP42604.
SMRiP42604. Positions 1-84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UxaA family.Curated

Phylogenomic databases

eggNOGiENOG4105CTX. Bacteria.
COG2721. LUCA.
HOGENOMiHOG000219064.
InParanoidiP42604.
KOiK01685.
OMAiGDDHENT.
PhylomeDBiP42604.

Family and domain databases

InterProiIPR007392. Gal/Altron_deHydtase_C.
IPR013974. SAF.
[Graphical view]
PfamiPF04295. GD_AH_C. 1 hit.
PF08666. SAF. 1 hit.
[Graphical view]
SMARTiSM00858. SAF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYIKIHALD NVAVALADLA EGTEVSVDNQ TVTLRQDVAR GHKFALTDIA
60 70 80 90 100
KGANVIKYGL PIGYALADIA AGVHVHAHNT RTNLSDLDQY RYQPDFQDLP
110 120 130 140 150
AQAADREVQI YRRANGDVGV RNELWILPTV GCVNGIARQI QNRFLKETNN
160 170 180 190 200
AEGTDGVFLF SHTYGCSQLG DDHINTRTML QNMVRHPNAG AVLVIGLGCE
210 220 230 240 250
NNQVAAFRET LGDIDPERVH FMICQQQDDE IEAGIEHLHQ LYNVMRNDKR
260 270 280 290 300
EPGKLSELKF GLECGGSDGL SGITANPMLG RFSDYVIANG GTTVLTEVPE
310 320 330 340 350
MFGAEQLLMD HCRDEATFEK LVTMVNDFKQ YFIAHDQPIY ENPSPGNKAG
360 370 380 390 400
GITTLEDKSL GCTQKAGSSV VVDVLRYGER LKTPGLNLLS APGNDAVATS
410 420 430 440 450
ALAGAGCHMV LFSTGRGTPY GGFVPTVKIA TNSELAAKKK HWIDFDAGQL
460 470 480 490
IHGKAMPQLL EEFIDTIVEF ANGKQTCNER NDFRELAIFK SGVTL
Length:495
Mass (Da):54,093
Last modified:November 1, 1997 - v3
Checksum:i480B21A93624FF32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13328 Genomic DNA. Translation: BAA18901.1.
U18997 Genomic DNA. Translation: AAA57893.1.
U00096 Genomic DNA. Translation: AAC76126.1.
AP009048 Genomic DNA. Translation: BAE77141.1.
PIRiH65097.
RefSeqiNP_417562.1. NC_000913.3.
WP_001199390.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76126; AAC76126; b3091.
BAE77141; BAE77141; BAE77141.
GeneIDi947603.
KEGGiecj:JW3062.
eco:b3091.
PATRICi32121600. VBIEscCol129921_3186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13328 Genomic DNA. Translation: BAA18901.1.
U18997 Genomic DNA. Translation: AAA57893.1.
U00096 Genomic DNA. Translation: AAC76126.1.
AP009048 Genomic DNA. Translation: BAE77141.1.
PIRiH65097.
RefSeqiNP_417562.1. NC_000913.3.
WP_001199390.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP42604.
SMRiP42604. Positions 1-84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262407. 11 interactions.
DIPiDIP-11105N.
IntActiP42604. 6 interactions.
STRINGi511145.b3091.

Proteomic databases

PaxDbiP42604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76126; AAC76126; b3091.
BAE77141; BAE77141; BAE77141.
GeneIDi947603.
KEGGiecj:JW3062.
eco:b3091.
PATRICi32121600. VBIEscCol129921_3186.

Organism-specific databases

EchoBASEiEB2592.
EcoGeneiEG12734. uxaA.

Phylogenomic databases

eggNOGiENOG4105CTX. Bacteria.
COG2721. LUCA.
HOGENOMiHOG000219064.
InParanoidiP42604.
KOiK01685.
OMAiGDDHENT.
PhylomeDBiP42604.

Enzyme and pathway databases

UniPathwayiUPA00246.
BioCyciEcoCyc:ALTRODEHYDRAT-MONOMER.
ECOL316407:JW3062-MONOMER.
MetaCyc:ALTRODEHYDRAT-MONOMER.

Miscellaneous databases

PROiP42604.

Family and domain databases

InterProiIPR007392. Gal/Altron_deHydtase_C.
IPR013974. SAF.
[Graphical view]
PfamiPF04295. GD_AH_C. 1 hit.
PF08666. SAF. 1 hit.
[Graphical view]
SMARTiSM00858. SAF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUXAA_ECOLI
AccessioniPrimary (citable) accession number: P42604
Secondary accession number(s): Q2M9B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.