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Protein

Ribosomal RNA large subunit methyltransferase G

Gene

rlmG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates the guanine in position 1835 (m2G1835) of 23S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N2-methylguanine(1835) in 23S rRNA.

GO - Molecular functioni

GO - Biological processi

  • rRNA base methylation Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7603-MONOMER
MetaCyc:G7603-MONOMER
BRENDAi2.1.1.174 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase G (EC:2.1.1.174)
Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG
Gene namesi
Name:rlmG
Synonyms:ygjO
Ordered Locus Names:b3084, JW5513
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12726 rlmG

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000974921 – 378Ribosomal RNA large subunit methyltransferase GAdd BLAST378

Proteomic databases

PaxDbiP42596
PRIDEiP42596

Interactioni

Protein-protein interaction databases

BioGridi4260876, 40 interactors
IntActiP42596, 1 interactor
STRINGi316385.ECDH10B_3259

Structurei

Secondary structure

1378
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 37Combined sources8
Turni38 – 41Combined sources4
Beta strandi48 – 51Combined sources4
Beta strandi54 – 56Combined sources3
Helixi57 – 61Combined sources5
Helixi63 – 65Combined sources3
Beta strandi68 – 72Combined sources5
Helixi74 – 86Combined sources13
Helixi91 – 93Combined sources3
Beta strandi94 – 98Combined sources5
Beta strandi108 – 113Combined sources6
Helixi118 – 129Combined sources12
Beta strandi136 – 143Combined sources8
Helixi144 – 146Combined sources3
Helixi149 – 158Combined sources10
Beta strandi162 – 164Combined sources3
Beta strandi171 – 176Combined sources6
Beta strandi190 – 194Combined sources5
Turni195 – 198Combined sources4
Beta strandi199 – 203Combined sources5
Helixi215 – 222Combined sources8
Beta strandi230 – 236Combined sources7
Helixi241 – 249Combined sources9
Beta strandi254 – 260Combined sources7
Helixi262 – 275Combined sources14
Helixi277 – 282Combined sources6
Beta strandi283 – 287Combined sources5
Turni290 – 293Combined sources4
Beta strandi299 – 304Combined sources6
Helixi318 – 329Combined sources12
Beta strandi330 – 341Combined sources12
Helixi346 – 354Combined sources9
Beta strandi358 – 362Combined sources5
Beta strandi364 – 372Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DCMX-ray2.30A9-373[»]
ProteinModelPortaliP42596
SMRiP42596
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D8J Bacteria
COG2813 LUCA
HOGENOMiHOG000246323
InParanoidiP42596
KOiK11391
OMAiNLQAWDA
PhylomeDBiP42596

Family and domain databases

HAMAPiMF_01859 23SrRNA_methyltr_G, 1 hit
InterProiView protein in InterPro
IPR002052 DNA_methylase_N6_adenine_CS
IPR017237 rRNA_m2G-MeTrfase_RsmD
IPR029063 SAM-dependent_MTases
IPR007848 Small_mtfrase_dom
PANTHERiPTHR18895:SF6 PTHR18895:SF6, 1 hit
PfamiView protein in Pfam
PF05175 MTS, 1 hit
PIRSFiPIRSF037565 RRNA_m2G_Mtase_RsmD_prd, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

P42596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHLDNGFRS LTLQRFPATD DVNPLQAWEA ADEYLLQQLD DTEIRGPVLI
60 70 80 90 100
LNDAFGALSC ALAEHKPYSI GDSYISELAT RENLRLNGID ESSVKFLDST
110 120 130 140 150
ADYPQQPGVV LIKVPKTLAL LEQQLRALRK VVTSDTRIIA GAKARDIHTS
160 170 180 190 200
TLELFEKVLG PTTTTLAWKK ARLINCTFNE PQLADAPQTV SWKLEGTDWT
210 220 230 240 250
IHNHANVFSR TGLDIGARFF MQHLPENLEG EIVDLGCGNG VIGLTLLDKN
260 270 280 290 300
PQAKVVFVDE SPMAVASSRL NVETNMPEAL DRCEFMINNA LSGVEPFRFN
310 320 330 340 350
AVLCNPPFHQ QHALTDNVAW EMFHHARRCL KINGELYIVA NRHLDYFHKL
360 370
KKIFGNCTTI ATNNKFVVLK AVKLGRRR
Length:378
Mass (Da):42,331
Last modified:December 1, 2000 - v2
Checksum:i0496E8BBD36FFB2C
GO

Sequence cautioni

The sequence AAA57885 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA57885.1 Different initiation.
U00096 Genomic DNA Translation: AAC76119.2
AP009048 Genomic DNA Translation: BAE77134.1
PIRiA65097
RefSeqiNP_417555.4, NC_000913.3
WP_000018695.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76119; AAC76119; b3084
BAE77134; BAE77134; BAE77134
GeneIDi947589
KEGGiecj:JW5513
eco:b3084
PATRICifig|511145.12.peg.3179

Similar proteinsi

Entry informationi

Entry nameiRLMG_ECOLI
AccessioniPrimary (citable) accession number: P42596
Secondary accession number(s): Q2M9C2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 1, 2000
Last modified: March 28, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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