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Protein

Ribosomal RNA large subunit methyltransferase G

Gene

rlmG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the guanine in position 1835 (m2G1835) of 23S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1835) in 23S rRNA.

GO - Molecular functioni

GO - Biological processi

  • rRNA base methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7603-MONOMER.
ECOL316407:JW5513-MONOMER.
MetaCyc:G7603-MONOMER.
BRENDAi2.1.1.174. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase G (EC:2.1.1.174)
Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG
Gene namesi
Name:rlmG
Synonyms:ygjO
Ordered Locus Names:b3084, JW5513
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12726. rlmG.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000974921 – 378Ribosomal RNA large subunit methyltransferase GAdd BLAST378

Proteomic databases

PaxDbiP42596.
PRIDEiP42596.

Interactioni

Protein-protein interaction databases

BioGridi4260876. 18 interactors.
IntActiP42596. 1 interactor.
STRINGi511145.b3084.

Structurei

Secondary structure

1378
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 37Combined sources8
Turni38 – 41Combined sources4
Beta strandi48 – 51Combined sources4
Beta strandi54 – 56Combined sources3
Helixi57 – 61Combined sources5
Helixi63 – 65Combined sources3
Beta strandi68 – 72Combined sources5
Helixi74 – 86Combined sources13
Helixi91 – 93Combined sources3
Beta strandi94 – 98Combined sources5
Beta strandi108 – 113Combined sources6
Helixi118 – 129Combined sources12
Beta strandi136 – 143Combined sources8
Helixi144 – 146Combined sources3
Helixi149 – 158Combined sources10
Beta strandi162 – 164Combined sources3
Beta strandi171 – 176Combined sources6
Beta strandi190 – 194Combined sources5
Turni195 – 198Combined sources4
Beta strandi199 – 203Combined sources5
Helixi215 – 222Combined sources8
Beta strandi230 – 236Combined sources7
Helixi241 – 249Combined sources9
Beta strandi254 – 260Combined sources7
Helixi262 – 275Combined sources14
Helixi277 – 282Combined sources6
Beta strandi283 – 287Combined sources5
Turni290 – 293Combined sources4
Beta strandi299 – 304Combined sources6
Helixi318 – 329Combined sources12
Beta strandi330 – 341Combined sources12
Helixi346 – 354Combined sources9
Beta strandi358 – 362Combined sources5
Beta strandi364 – 372Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DCMX-ray2.30A9-373[»]
ProteinModelPortaliP42596.
SMRiP42596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D8J. Bacteria.
COG2813. LUCA.
HOGENOMiHOG000246323.
InParanoidiP42596.
KOiK11391.
OMAiFHQQQVV.
PhylomeDBiP42596.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01859. 23SrRNA_methyltr_G. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR017237. rRNA_m2G-MeTrfase_RsmD.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
[Graphical view]
PfamiPF05175. MTS. 1 hit.
[Graphical view]
PIRSFiPIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P42596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHLDNGFRS LTLQRFPATD DVNPLQAWEA ADEYLLQQLD DTEIRGPVLI
60 70 80 90 100
LNDAFGALSC ALAEHKPYSI GDSYISELAT RENLRLNGID ESSVKFLDST
110 120 130 140 150
ADYPQQPGVV LIKVPKTLAL LEQQLRALRK VVTSDTRIIA GAKARDIHTS
160 170 180 190 200
TLELFEKVLG PTTTTLAWKK ARLINCTFNE PQLADAPQTV SWKLEGTDWT
210 220 230 240 250
IHNHANVFSR TGLDIGARFF MQHLPENLEG EIVDLGCGNG VIGLTLLDKN
260 270 280 290 300
PQAKVVFVDE SPMAVASSRL NVETNMPEAL DRCEFMINNA LSGVEPFRFN
310 320 330 340 350
AVLCNPPFHQ QHALTDNVAW EMFHHARRCL KINGELYIVA NRHLDYFHKL
360 370
KKIFGNCTTI ATNNKFVVLK AVKLGRRR
Length:378
Mass (Da):42,331
Last modified:December 1, 2000 - v2
Checksum:i0496E8BBD36FFB2C
GO

Sequence cautioni

The sequence AAA57885 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57885.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76119.2.
AP009048 Genomic DNA. Translation: BAE77134.1.
PIRiA65097.
RefSeqiNP_417555.4. NC_000913.3.
WP_000018695.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76119; AAC76119; b3084.
BAE77134; BAE77134; BAE77134.
GeneIDi947589.
KEGGiecj:JW5513.
eco:b3084.
PATRICi32121586. VBIEscCol129921_3179.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57885.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76119.2.
AP009048 Genomic DNA. Translation: BAE77134.1.
PIRiA65097.
RefSeqiNP_417555.4. NC_000913.3.
WP_000018695.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DCMX-ray2.30A9-373[»]
ProteinModelPortaliP42596.
SMRiP42596.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260876. 18 interactors.
IntActiP42596. 1 interactor.
STRINGi511145.b3084.

Proteomic databases

PaxDbiP42596.
PRIDEiP42596.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76119; AAC76119; b3084.
BAE77134; BAE77134; BAE77134.
GeneIDi947589.
KEGGiecj:JW5513.
eco:b3084.
PATRICi32121586. VBIEscCol129921_3179.

Organism-specific databases

EchoBASEiEB2585.
EcoGeneiEG12726. rlmG.

Phylogenomic databases

eggNOGiENOG4105D8J. Bacteria.
COG2813. LUCA.
HOGENOMiHOG000246323.
InParanoidiP42596.
KOiK11391.
OMAiFHQQQVV.
PhylomeDBiP42596.

Enzyme and pathway databases

BioCyciEcoCyc:G7603-MONOMER.
ECOL316407:JW5513-MONOMER.
MetaCyc:G7603-MONOMER.
BRENDAi2.1.1.174. 2026.

Miscellaneous databases

PROiP42596.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01859. 23SrRNA_methyltr_G. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR017237. rRNA_m2G-MeTrfase_RsmD.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
[Graphical view]
PfamiPF05175. MTS. 1 hit.
[Graphical view]
PIRSFiPIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRLMG_ECOLI
AccessioniPrimary (citable) accession number: P42596
Secondary accession number(s): Q2M9C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.