P42596 (RLMG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Synonyms:	ygjO
Ordered Locus Names:	b3084, JW5513

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA. Ref.3
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.
Sequence caution	The sequence AAA57885.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	rRNA base methylation Inferred from mutant phenotype Ref.3. Source: EcoCyc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from direct assay Ref.3. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000097492

Sequences

	Sequence		Length	Mass (Da)
	P42596 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 0496E8BBD36FFB2C	FASTA	378	42,331
	10 20 30 40 50 60 MSHLDNGFRS LTLQRFPATD DVNPLQAWEA ADEYLLQQLD DTEIRGPVLI LNDAFGALSC 70 80 90 100 110 120 ALAEHKPYSI GDSYISELAT RENLRLNGID ESSVKFLDST ADYPQQPGVV LIKVPKTLAL 130 140 150 160 170 180 LEQQLRALRK VVTSDTRIIA GAKARDIHTS TLELFEKVLG PTTTTLAWKK ARLINCTFNE 190 200 210 220 230 240 PQLADAPQTV SWKLEGTDWT IHNHANVFSR TGLDIGARFF MQHLPENLEG EIVDLGCGNG 250 260 270 280 290 300 VIGLTLLDKN PQAKVVFVDE SPMAVASSRL NVETNMPEAL DRCEFMINNA LSGVEPFRFN 310 320 330 340 350 360 AVLCNPPFHQ QHALTDNVAW EMFHHARRCL KINGELYIVA NRHLDYFHKL KKIFGNCTTI 370 ATNNKFVVLK AVKLGRRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Identification of Escherichia coli m2G methyltransferases: II. The ygjO gene encodes a methyltransferase specific for G1835 of the 23 S rRNA." Sergiev P.V., Lesnyak D.V., Bogdanov A.A., Dontsova O.A. J. Mol. Biol. 364:26-31(2006) [PubMed: 17010380] [Abstract] Cited for: FUNCTION AS RRNA METHYLTRANSFERASE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA57885.1. Different initiation. U00096 Genomic DNA. Translation: AAC76119.2. AP009048 Genomic DNA. Translation: BAE77134.1.
PIR	A65097.
RefSeq	NP_417555.4. NC_000913.2.
3D structure databases
ProteinModelPortal	P42596.
SMR	P42596. Positions 27-372.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004554; EBESCP00000004554; EBESCG00000003717. EBESCT00000018310; EBESCP00000017601; EBESCG00000017364.
GeneID	947589.
GenomeReviews	Gene locus JW5513 in contig AP009048_GR. Gene locus b3084 in contig U00096_GR.
KEGG	ecj:JW5513. eco:b3084.
PATRIC	32121586. VBIEscCol129921_3179.
Organism-specific databases
EchoBASE	EB2585.
EcoGene	EG12726. rlmG.
Phylogenomic databases
eggNOG	COG2813.
GeneTree	EBGT00050000009812.
HOGENOM	HBG321931.
OMA	NRHLGYH.
PhylomeDB	P42596.
ProtClustDB	PRK15001.
Enzyme and pathway databases
BioCyc	EcoCyc:G7603-MONOMER. MetaCyc:G7603-MONOMER.
Gene expression databases
Genevestigator	P42596.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K11391.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_ECOLI

Accession

Primary (citable) accession number: P42596
Secondary accession number(s): Q2M9C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program