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Protein

Glucosidase YgjK

Gene

ygjK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Glucoside hydrolase that cleaves the alpha-1,3-glucosidic linkage in nigerose. Has very low activity towards maltooligosaccharides, soluble starch, nigerotriose, kojibiose and trehalose.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi454Calcium1
Metal bindingi456Calcium1
Metal bindingi458Calcium1
Metal bindingi460Calcium; via carbonyl oxygen1
Metal bindingi462Calcium1
Active sitei524Proton donor/acceptorBy similarity1
Metal bindingi572Calcium1
Active sitei750Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • alpha,alpha-trehalase activity Source: GO_Central
  • glucosidase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • trehalose catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7599-MONOMER.
ECOL316407:JW3051-MONOMER.
MetaCyc:G7599-MONOMER.

Protein family/group databases

CAZyiGH63. Glycoside Hydrolase Family 63.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosidase YgjK (EC:3.2.1.-)
Gene namesi
Name:ygjK
Ordered Locus Names:b3080, JW3051
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12722. ygjK.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000001390623 – 783Glucosidase YgjKAdd BLAST761

Proteomic databases

PaxDbiP42592.
PRIDEiP42592.

Interactioni

Protein-protein interaction databases

BioGridi4259266. 13 interactors.
IntActiP42592. 5 interactors.
STRINGi511145.b3080.

Structurei

Secondary structure

1783
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 27Combined sources3
Beta strandi40 – 44Combined sources5
Beta strandi48 – 52Combined sources5
Beta strandi61 – 64Combined sources4
Turni70 – 74Combined sources5
Beta strandi79 – 82Combined sources4
Beta strandi84 – 101Combined sources18
Beta strandi109 – 115Combined sources7
Beta strandi118 – 124Combined sources7
Beta strandi126 – 138Combined sources13
Beta strandi141 – 152Combined sources12
Beta strandi154 – 162Combined sources9
Beta strandi165 – 169Combined sources5
Beta strandi172 – 174Combined sources3
Helixi179 – 182Combined sources4
Turni184 – 186Combined sources3
Beta strandi189 – 193Combined sources5
Beta strandi196 – 200Combined sources5
Beta strandi204 – 206Combined sources3
Turni207 – 209Combined sources3
Beta strandi210 – 212Combined sources3
Beta strandi217 – 224Combined sources8
Beta strandi227 – 231Combined sources5
Beta strandi234 – 241Combined sources8
Beta strandi243 – 254Combined sources12
Helixi257 – 272Combined sources16
Helixi274 – 292Combined sources19
Helixi300 – 315Combined sources16
Beta strandi316 – 318Combined sources3
Turni336 – 338Combined sources3
Helixi345 – 355Combined sources11
Turni356 – 358Combined sources3
Helixi360 – 371Combined sources12
Helixi383 – 385Combined sources3
Beta strandi391 – 393Combined sources3
Helixi399 – 401Combined sources3
Helixi418 – 429Combined sources12
Helixi432 – 452Combined sources21
Beta strandi457 – 460Combined sources4
Beta strandi464 – 466Combined sources3
Turni469 – 471Combined sources3
Beta strandi478 – 484Combined sources7
Beta strandi487 – 492Combined sources6
Helixi494 – 503Combined sources10
Beta strandi507 – 510Combined sources4
Helixi512 – 521Combined sources10
Helixi527 – 529Combined sources3
Helixi534 – 542Combined sources9
Helixi547 – 550Combined sources4
Beta strandi554 – 558Combined sources5
Beta strandi564 – 572Combined sources9
Helixi574 – 593Combined sources20
Helixi597 – 617Combined sources21
Turni621 – 624Combined sources4
Helixi646 – 648Combined sources3
Helixi652 – 655Combined sources4
Helixi656 – 659Combined sources4
Helixi665 – 675Combined sources11
Turni678 – 681Combined sources4
Beta strandi683 – 685Combined sources3
Turni702 – 705Combined sources4
Helixi709 – 721Combined sources13
Helixi725 – 738Combined sources14
Turni740 – 743Combined sources4
Beta strandi744 – 746Combined sources3
Beta strandi750 – 752Combined sources3
Turni754 – 756Combined sources3
Beta strandi759 – 761Combined sources3
Helixi766 – 778Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D3IX-ray1.78A/B23-783[»]
3W7SX-ray1.90A/B24-783[»]
3W7TX-ray1.50A/B24-783[»]
3W7UX-ray1.99A/B24-783[»]
3W7WX-ray2.00A/B24-783[»]
3W7XX-ray2.70A/B24-783[»]
5CA3X-ray1.80A/B24-783[»]
5GW7X-ray2.20A/B24-783[»]
ProteinModelPortaliP42592.
SMRiP42592.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42592.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 254N-terminal domainAdd BLAST231
Regioni254 – 299LinkerAdd BLAST46
Regioni300 – 783A domainAdd BLAST484

Sequence similaritiesi

Belongs to the glycosyl hydrolase 63 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107WCA. Bacteria.
ENOG410ZVE6. LUCA.
HOGENOMiHOG000281137.
InParanoidiP42592.
KOiK03931.
OMAiWLEEMYP.
PhylomeDBiP42592.

Family and domain databases

InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR001661. Glyco_hydro_37.
[Graphical view]
PANTHERiPTHR23403. PTHR23403. 2 hits.
PfamiPF01204. Trehalase. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42592-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIKTILTPV TCALLISFSA HAANADNYKN VINRTGAPQY MKDYDYDDHQ
60 70 80 90 100
RFNPFFDLGA WHGHLLPDGP NTMGGFPGVA LLTEEYINFM ASNFDRLTVW
110 120 130 140 150
QDGKKVDFTL EAYSIPGALV QKLTAKDVQV EMTLRFATPR TSLLETKITS
160 170 180 190 200
NKPLDLVWDG ELLEKLEAKE GKPLSDKTIA GEYPDYQRKI SATRDGLKVT
210 220 230 240 250
FGKVRATWDL LTSGESEYQV HKSLPVQTEI NGNRFTSKAH INGSTTLYTT
260 270 280 290 300
YSHLLTAQEV SKEQMQIRDI LARPAFYLTA SQQRWEEYLK KGLTNPDATP
310 320 330 340 350
EQTRVAVKAI ETLNGNWRSP GGAVKFNTVT PSVTGRWFSG NQTWPWDTWK
360 370 380 390 400
QAFAMAHFNP DIAKENIRAV FSWQIQPGDS VRPQDVGFVP DLIAWNLSPE
410 420 430 440 450
RGGDGGNWNE RNTKPSLAAW SVMEVYNVTQ DKTWVAEMYP KLVAYHDWWL
460 470 480 490 500
RNRDHNGNGV PEYGATRDKA HNTESGEMLF TVKKGDKEET QSGLNNYARV
510 520 530 540 550
VEKGQYDSLE IPAQVAASWE SGRDDAAVFG FIDKEQLDKY VANGGKRSDW
560 570 580 590 600
TVKFAENRSQ DGTLLGYSLL QESVDQASYM YSDNHYLAEM ATILGKPEEA
610 620 630 640 650
KRYRQLAQQL ADYINTCMFD PTTQFYYDVR IEDKPLANGC AGKPIVERGK
660 670 680 690 700
GPEGWSPLFN GAATQANADA VVKVMLDPKE FNTFVPLGTA ALTNPAFGAD
710 720 730 740 750
IYWRGRVWVD QFWFGLKGME RYGYRDDALK LADTFFRHAK GLTADGPIQE
760 770 780
NYNPLTGAQQ GAPNFSWSAA HLYMLYNDFF RKQ
Length:783
Mass (Da):88,321
Last modified:November 1, 1995 - v1
Checksum:iAE4C37BFB78EC82B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57881.1.
U00096 Genomic DNA. Translation: AAC76115.1.
AP009048 Genomic DNA. Translation: BAE77130.1.
PIRiE65096.
RefSeqiNP_417551.1. NC_000913.3.
WP_000695487.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76115; AAC76115; b3080.
BAE77130; BAE77130; BAE77130.
GeneIDi947596.
KEGGiecj:JW3051.
eco:b3080.
PATRICi32121576. VBIEscCol129921_3174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57881.1.
U00096 Genomic DNA. Translation: AAC76115.1.
AP009048 Genomic DNA. Translation: BAE77130.1.
PIRiE65096.
RefSeqiNP_417551.1. NC_000913.3.
WP_000695487.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D3IX-ray1.78A/B23-783[»]
3W7SX-ray1.90A/B24-783[»]
3W7TX-ray1.50A/B24-783[»]
3W7UX-ray1.99A/B24-783[»]
3W7WX-ray2.00A/B24-783[»]
3W7XX-ray2.70A/B24-783[»]
5CA3X-ray1.80A/B24-783[»]
5GW7X-ray2.20A/B24-783[»]
ProteinModelPortaliP42592.
SMRiP42592.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259266. 13 interactors.
IntActiP42592. 5 interactors.
STRINGi511145.b3080.

Protein family/group databases

CAZyiGH63. Glycoside Hydrolase Family 63.

Proteomic databases

PaxDbiP42592.
PRIDEiP42592.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76115; AAC76115; b3080.
BAE77130; BAE77130; BAE77130.
GeneIDi947596.
KEGGiecj:JW3051.
eco:b3080.
PATRICi32121576. VBIEscCol129921_3174.

Organism-specific databases

EchoBASEiEB2581.
EcoGeneiEG12722. ygjK.

Phylogenomic databases

eggNOGiENOG4107WCA. Bacteria.
ENOG410ZVE6. LUCA.
HOGENOMiHOG000281137.
InParanoidiP42592.
KOiK03931.
OMAiWLEEMYP.
PhylomeDBiP42592.

Enzyme and pathway databases

BioCyciEcoCyc:G7599-MONOMER.
ECOL316407:JW3051-MONOMER.
MetaCyc:G7599-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP42592.
PROiP42592.

Family and domain databases

InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR001661. Glyco_hydro_37.
[Graphical view]
PANTHERiPTHR23403. PTHR23403. 2 hits.
PfamiPF01204. Trehalase. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiYGJK_ECOLI
AccessioniPrimary (citable) accession number: P42592
Secondary accession number(s): Q2M9C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.