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Protein

Glucosidase YgjK

Gene

ygjK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Glucoside hydrolase that cleaves the alpha-1,3-glucosidic linkage in nigerose. Has very low activity towards maltooligosaccharides, soluble starch, nigerotriose, kojibiose and trehalose.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi454 – 4541Calcium
Metal bindingi456 – 4561Calcium
Metal bindingi458 – 4581Calcium
Metal bindingi460 – 4601Calcium; via carbonyl oxygen
Metal bindingi462 – 4621Calcium
Active sitei524 – 5241Proton donor/acceptorBy similarity
Metal bindingi572 – 5721Calcium
Active sitei750 – 7501Proton donor/acceptorBy similarity

GO - Molecular functioni

  • alpha,alpha-trehalase activity Source: GO_Central
  • glucosidase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • trehalose catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7599-MONOMER.
ECOL316407:JW3051-MONOMER.
MetaCyc:G7599-MONOMER.

Protein family/group databases

CAZyiGH63. Glycoside Hydrolase Family 63.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosidase YgjK (EC:3.2.1.-)
Gene namesi
Name:ygjK
Ordered Locus Names:b3080, JW3051
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12722. ygjK.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 783761Glucosidase YgjKPRO_0000013906Add
BLAST

Proteomic databases

PaxDbiP42592.
PRIDEiP42592.

Interactioni

Protein-protein interaction databases

BioGridi4259266. 13 interactions.
IntActiP42592. 5 interactions.
STRINGi511145.b3080.

Structurei

Secondary structure

1
783
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi25 – 273Combined sources
Beta strandi40 – 445Combined sources
Beta strandi48 – 525Combined sources
Beta strandi61 – 644Combined sources
Turni70 – 745Combined sources
Beta strandi79 – 824Combined sources
Beta strandi84 – 10118Combined sources
Beta strandi109 – 1157Combined sources
Beta strandi118 – 1247Combined sources
Beta strandi126 – 13813Combined sources
Beta strandi141 – 15212Combined sources
Beta strandi154 – 1629Combined sources
Beta strandi165 – 1695Combined sources
Beta strandi172 – 1743Combined sources
Helixi179 – 1824Combined sources
Turni184 – 1863Combined sources
Beta strandi189 – 1935Combined sources
Beta strandi196 – 2005Combined sources
Beta strandi204 – 2063Combined sources
Turni207 – 2093Combined sources
Beta strandi210 – 2123Combined sources
Beta strandi217 – 2248Combined sources
Beta strandi227 – 2315Combined sources
Beta strandi234 – 2418Combined sources
Beta strandi243 – 25412Combined sources
Helixi257 – 27216Combined sources
Helixi274 – 29219Combined sources
Helixi300 – 31516Combined sources
Beta strandi316 – 3183Combined sources
Turni336 – 3383Combined sources
Helixi345 – 35511Combined sources
Turni356 – 3583Combined sources
Helixi360 – 37112Combined sources
Helixi383 – 3853Combined sources
Beta strandi391 – 3933Combined sources
Helixi399 – 4013Combined sources
Helixi418 – 42912Combined sources
Helixi432 – 45221Combined sources
Beta strandi457 – 4604Combined sources
Beta strandi464 – 4663Combined sources
Turni469 – 4713Combined sources
Beta strandi478 – 4847Combined sources
Beta strandi487 – 4926Combined sources
Helixi494 – 50310Combined sources
Beta strandi507 – 5104Combined sources
Helixi512 – 52110Combined sources
Helixi527 – 5293Combined sources
Helixi534 – 5429Combined sources
Helixi547 – 5504Combined sources
Beta strandi554 – 5585Combined sources
Beta strandi564 – 5729Combined sources
Helixi574 – 59320Combined sources
Helixi597 – 61721Combined sources
Turni621 – 6244Combined sources
Helixi646 – 6483Combined sources
Helixi652 – 6554Combined sources
Helixi656 – 6594Combined sources
Helixi665 – 67511Combined sources
Turni678 – 6814Combined sources
Beta strandi683 – 6853Combined sources
Turni702 – 7054Combined sources
Helixi709 – 72113Combined sources
Helixi725 – 73814Combined sources
Turni740 – 7434Combined sources
Beta strandi744 – 7463Combined sources
Beta strandi750 – 7523Combined sources
Turni754 – 7563Combined sources
Beta strandi759 – 7613Combined sources
Helixi766 – 77813Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3D3IX-ray1.78A/B23-783[»]
3W7SX-ray1.90A/B24-783[»]
3W7TX-ray1.50A/B24-783[»]
3W7UX-ray1.99A/B24-783[»]
3W7WX-ray2.00A/B24-783[»]
3W7XX-ray2.70A/B24-783[»]
ProteinModelPortaliP42592.
SMRiP42592. Positions 24-783.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42592.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 254231N-terminal domainAdd
BLAST
Regioni254 – 29946LinkerAdd
BLAST
Regioni300 – 783484A domainAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 63 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107WCA. Bacteria.
ENOG410ZVE6. LUCA.
HOGENOMiHOG000281137.
InParanoidiP42592.
KOiK03931.
OMAiWLEEMYP.
PhylomeDBiP42592.

Family and domain databases

InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR001661. Glyco_hydro_37.
[Graphical view]
PANTHERiPTHR23403. PTHR23403. 2 hits.
PfamiPF01204. Trehalase. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42592-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIKTILTPV TCALLISFSA HAANADNYKN VINRTGAPQY MKDYDYDDHQ
60 70 80 90 100
RFNPFFDLGA WHGHLLPDGP NTMGGFPGVA LLTEEYINFM ASNFDRLTVW
110 120 130 140 150
QDGKKVDFTL EAYSIPGALV QKLTAKDVQV EMTLRFATPR TSLLETKITS
160 170 180 190 200
NKPLDLVWDG ELLEKLEAKE GKPLSDKTIA GEYPDYQRKI SATRDGLKVT
210 220 230 240 250
FGKVRATWDL LTSGESEYQV HKSLPVQTEI NGNRFTSKAH INGSTTLYTT
260 270 280 290 300
YSHLLTAQEV SKEQMQIRDI LARPAFYLTA SQQRWEEYLK KGLTNPDATP
310 320 330 340 350
EQTRVAVKAI ETLNGNWRSP GGAVKFNTVT PSVTGRWFSG NQTWPWDTWK
360 370 380 390 400
QAFAMAHFNP DIAKENIRAV FSWQIQPGDS VRPQDVGFVP DLIAWNLSPE
410 420 430 440 450
RGGDGGNWNE RNTKPSLAAW SVMEVYNVTQ DKTWVAEMYP KLVAYHDWWL
460 470 480 490 500
RNRDHNGNGV PEYGATRDKA HNTESGEMLF TVKKGDKEET QSGLNNYARV
510 520 530 540 550
VEKGQYDSLE IPAQVAASWE SGRDDAAVFG FIDKEQLDKY VANGGKRSDW
560 570 580 590 600
TVKFAENRSQ DGTLLGYSLL QESVDQASYM YSDNHYLAEM ATILGKPEEA
610 620 630 640 650
KRYRQLAQQL ADYINTCMFD PTTQFYYDVR IEDKPLANGC AGKPIVERGK
660 670 680 690 700
GPEGWSPLFN GAATQANADA VVKVMLDPKE FNTFVPLGTA ALTNPAFGAD
710 720 730 740 750
IYWRGRVWVD QFWFGLKGME RYGYRDDALK LADTFFRHAK GLTADGPIQE
760 770 780
NYNPLTGAQQ GAPNFSWSAA HLYMLYNDFF RKQ
Length:783
Mass (Da):88,321
Last modified:November 1, 1995 - v1
Checksum:iAE4C37BFB78EC82B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57881.1.
U00096 Genomic DNA. Translation: AAC76115.1.
AP009048 Genomic DNA. Translation: BAE77130.1.
PIRiE65096.
RefSeqiNP_417551.1. NC_000913.3.
WP_000695487.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76115; AAC76115; b3080.
BAE77130; BAE77130; BAE77130.
GeneIDi947596.
KEGGiecj:JW3051.
eco:b3080.
PATRICi32121576. VBIEscCol129921_3174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57881.1.
U00096 Genomic DNA. Translation: AAC76115.1.
AP009048 Genomic DNA. Translation: BAE77130.1.
PIRiE65096.
RefSeqiNP_417551.1. NC_000913.3.
WP_000695487.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3D3IX-ray1.78A/B23-783[»]
3W7SX-ray1.90A/B24-783[»]
3W7TX-ray1.50A/B24-783[»]
3W7UX-ray1.99A/B24-783[»]
3W7WX-ray2.00A/B24-783[»]
3W7XX-ray2.70A/B24-783[»]
ProteinModelPortaliP42592.
SMRiP42592. Positions 24-783.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259266. 13 interactions.
IntActiP42592. 5 interactions.
STRINGi511145.b3080.

Protein family/group databases

CAZyiGH63. Glycoside Hydrolase Family 63.

Proteomic databases

PaxDbiP42592.
PRIDEiP42592.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76115; AAC76115; b3080.
BAE77130; BAE77130; BAE77130.
GeneIDi947596.
KEGGiecj:JW3051.
eco:b3080.
PATRICi32121576. VBIEscCol129921_3174.

Organism-specific databases

EchoBASEiEB2581.
EcoGeneiEG12722. ygjK.

Phylogenomic databases

eggNOGiENOG4107WCA. Bacteria.
ENOG410ZVE6. LUCA.
HOGENOMiHOG000281137.
InParanoidiP42592.
KOiK03931.
OMAiWLEEMYP.
PhylomeDBiP42592.

Enzyme and pathway databases

BioCyciEcoCyc:G7599-MONOMER.
ECOL316407:JW3051-MONOMER.
MetaCyc:G7599-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP42592.
PROiP42592.

Family and domain databases

InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR001661. Glyco_hydro_37.
[Graphical view]
PANTHERiPTHR23403. PTHR23403. 2 hits.
PfamiPF01204. Trehalase. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiYGJK_ECOLI
AccessioniPrimary (citable) accession number: P42592
Secondary accession number(s): Q2M9C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.