Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homeobox protein Nkx-2.2

Gene

Nkx2-2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi128 – 187HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • DNA binding transcription factor activity Source: MGI
  • proximal promoter sequence-specific DNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: MGI
  • transcription coactivator activity Source: UniProtKB
  • transcription factor binding Source: MGI

GO - Biological processi

  • astrocyte differentiation Source: MGI
  • cell development Source: MGI
  • cell differentiation Source: MGI
  • digestive tract development Source: MGI
  • endocrine pancreas development Source: MGI
  • negative regulation of neuron differentiation Source: MGI
  • nervous system development Source: MGI
  • neuron fate specification Source: MGI
  • oligodendrocyte development Source: MGI
  • optic nerve development Source: Ensembl
  • pancreatic A cell fate commitment Source: MGI
  • pancreatic PP cell fate commitment Source: MGI
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of DNA binding transcription factor activity Source: UniProtKB
  • positive regulation of gene expression Source: MGI
  • positive regulation of neuron differentiation Source: MGI
  • positive regulation of oligodendrocyte differentiation Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of cell differentiation Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • response to glucose Source: Ensembl
  • response to progesterone Source: Ensembl
  • smoothened signaling pathway Source: MGI
  • spinal cord motor neuron differentiation Source: MGI
  • spinal cord oligodendrocyte cell differentiation Source: MGI
  • spinal cord oligodendrocyte cell fate specification Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • type B pancreatic cell development Source: MGI
  • type B pancreatic cell fate commitment Source: MGI
  • ventral spinal cord interneuron fate determination Source: MGI

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-210746 Regulation of gene expression in endocrine-committed (NEUROG3+) progenitor cells

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Nkx-2.2
Alternative name(s):
Homeobox protein NK-2 homolog B
Gene namesi
Name:Nkx2-2
Synonyms:Nkx-2.2, Nkx2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:97347 Nkx2-2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi184K → I: Inhibits DNA-binding activity and transcriptional activation of NEUROD1 expression. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000489311 – 273Homeobox protein Nkx-2.2Add BLAST273

Proteomic databases

PaxDbiP42586
PeptideAtlasiP42586
PRIDEiP42586

PTM databases

iPTMnetiP42586
PhosphoSitePlusiP42586

Expressioni

Tissue specificityi

Expressed in restricted areas of the developing CNS: the hindbrain and forebrain, and pancreas.

Developmental stagei

Expressed during embryogenesis.

Gene expression databases

BgeeiENSMUSG00000027434
ExpressionAtlasiP42586 baseline and differential
GenevisibleiP42586 MM

Interactioni

Subunit structurei

Interacts with OLIG2.1 Publication

GO - Molecular functioni

  • transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi2017764 interactors.
IntActiP42586 1 interactor.
MINTiP42586
STRINGi10090.ENSMUSP00000069666

Structurei

3D structure databases

ProteinModelPortaliP42586
SMRiP42586
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The homeodomain is essential for interaction with OLIG2.

Sequence similaritiesi

Belongs to the NK-2 homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0842 Eukaryota
ENOG410XR21 LUCA
GeneTreeiENSGT00910000144003
HOGENOMiHOG000231923
HOVERGENiHBG006689
InParanoidiP42586
KOiK08029
OrthoDBiEOG091G0LZ2
PhylomeDBiP42586
TreeFamiTF351204

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR020479 Homeobox_metazoa
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PRINTSiPR00024 HOMEOBOX
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

P42586-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLTNTKTGF SVKDILDLPD TNDEDGSVAE GPEEESEGPE PAKRAGPLGQ
60 70 80 90 100
GALDAVQSLP LKSPFYDSSD NPYTRWLAST EGLQYSLHGL AASAPPQDSS
110 120 130 140 150
SKSPEPSADE SPDNDKETQG GGGDAGKKRK RRVLFSKAQT YELERRFRQQ
160 170 180 190 200
RYLSAPEREH LASLIRLTPT QVKIWFQNHR YKMKRARAEK GMEVTPLPSP
210 220 230 240 250
RRVAVPVLVR DGKPCHALKA QDLAAATFQA GIPFSAYSAQ SLQHMQYNAQ
260 270
YSSASTPQYP TAHPLVQAQQ WTW
Length:273
Mass (Da):30,126
Last modified:October 1, 1996 - v2
Checksum:i1F3DBB8127667F19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31566 mRNA Translation: AAA79303.1
S83259 Genomic DNA No translation available.
CCDSiCCDS16834.1
PIRiJC4634
RefSeqiNP_035049.1, NM_010919.2
XP_006498961.1, XM_006498898.2
XP_006498962.1, XM_006498899.2
XP_006498963.1, XM_006498900.3
XP_011237651.1, XM_011239349.2
UniGeneiMm.330639

Genome annotation databases

EnsembliENSMUST00000067075; ENSMUSP00000069666; ENSMUSG00000027434
GeneIDi18088
KEGGimmu:18088
UCSCiuc008msu.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNKX22_MOUSE
AccessioniPrimary (citable) accession number: P42586
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome