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P42586 (NKX22_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein Nkx-2.2
Alternative name(s):
Homeobox protein NK-2 homolog B
Gene names
Name:Nkx2-2
Synonyms:Nkx-2.2, Nkx2b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter. Ref.5

Subunit structure

Interacts with OLIG2. Ref.4

Subcellular location

Nucleus Ref.4.

Tissue specificity

Expressed in restricted areas of the developing CNS: the hindbrain and forebrain, and pancreas.

Developmental stage

Expressed during embryogenesis.

Domain

The homeodomain is essential for interaction with OLIG2.

Sequence similarities

Belongs to the NK-2 homeobox family.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processastrocyte differentiation

Inferred from mutant phenotype PubMed 19258013. Source: MGI

cell development

Inferred from mutant phenotype PubMed 11526078. Source: MGI

cell differentiation

Inferred from mutant phenotype PubMed 18987169. Source: MGI

digestive tract development

Inferred from mutant phenotype PubMed 18987169. Source: MGI

endocrine pancreas development

Inferred from mutant phenotype PubMed 9584121. Source: MGI

negative regulation of neuron differentiation

Inferred from direct assay PubMed 10830170. Source: MGI

nervous system development

Inferred from direct assay Ref.4. Source: MGI

neuron fate specification

Inferred from genetic interaction. Source: MGI

oligodendrocyte development

Inferred from mutant phenotype PubMed 11526078. Source: MGI

optic nerve development

Inferred from electronic annotation. Source: Ensembl

pancreatic A cell fate commitment

Inferred from mutant phenotype PubMed 17202186. Source: MGI

pancreatic PP cell fate commitment

Inferred from genetic interaction PubMed 17988662. Source: MGI

positive regulation of cell differentiation

Inferred from mutant phenotype PubMed 14729487. Source: MGI

positive regulation of neuron differentiation

Inferred from direct assay PubMed 10830170. Source: MGI

positive regulation of oligodendrocyte differentiation

Inferred from genetic interaction PubMed 11566099. Source: MGI

positive regulation of sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.5. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 11526078PubMed 14701942. Source: MGI

regulation of cell differentiation

Inferred from mutant phenotype PubMed 14970313. Source: MGI

regulation of transcription, DNA-templated

Inferred from direct assay Ref.4. Source: MGI

response to glucose

Inferred from electronic annotation. Source: Ensembl

response to progesterone

Inferred from electronic annotation. Source: Ensembl

smoothened signaling pathway

Inferred from direct assay PubMed 18590716. Source: MGI

spinal cord motor neuron differentiation

Inferred from mutant phenotype PubMed 10217145. Source: MGI

spinal cord oligodendrocyte cell differentiation

Inferred from mutant phenotype PubMed 11526078. Source: MGI

spinal cord oligodendrocyte cell fate specification

Inferred from direct assay PubMed 18287202. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

type B pancreatic cell development

Inferred from direct assay PubMed 17456846. Source: MGI

type B pancreatic cell fate commitment

Inferred from mutant phenotype PubMed 17202186PubMed 17988662. Source: MGI

ventral spinal cord interneuron fate determination

Inferred from mutant phenotype PubMed 10217145. Source: MGI

   Cellular_componentintracellular

Inferred from direct assay PubMed 15793245. Source: MGI

nucleoplasm

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay Ref.4PubMed 16306355PubMed 18076286PubMed 9230312. Source: MGI

   Molecular_functionchromatin binding

Inferred from direct assay Ref.5. Source: UniProtKB

core promoter proximal region DNA binding

Inferred from direct assay Ref.5. Source: UniProtKB

protein binding

Inferred from physical interaction Ref.4PubMed 17202186. Source: MGI

sequence-specific DNA binding

Inferred from direct assay PubMed 14701942. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.4PubMed 14701942. Source: MGI

transcription coactivator activity

Inferred from direct assay Ref.5. Source: UniProtKB

transcription factor binding

Inferred from physical interaction PubMed 16887115. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273Homeobox protein Nkx-2.2
PRO_0000048931

Regions

DNA binding128 – 18760Homeobox Ref.5

Experimental info

Mutagenesis1841K → I: Inhibits DNA-binding activity and transcriptional activation of NEUROD1 expression. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P42586 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 1F3DBB8127667F19

FASTA27330,126
        10         20         30         40         50         60 
MSLTNTKTGF SVKDILDLPD TNDEDGSVAE GPEEESEGPE PAKRAGPLGQ GALDAVQSLP 

        70         80         90        100        110        120 
LKSPFYDSSD NPYTRWLAST EGLQYSLHGL AASAPPQDSS SKSPEPSADE SPDNDKETQG 

       130        140        150        160        170        180 
GGGDAGKKRK RRVLFSKAQT YELERRFRQQ RYLSAPEREH LASLIRLTPT QVKIWFQNHR 

       190        200        210        220        230        240 
YKMKRARAEK GMEVTPLPSP RRVAVPVLVR DGKPCHALKA QDLAAATFQA GIPFSAYSAQ 

       250        260        270 
SLQHMQYNAQ YSSASTPQYP TAHPLVQAQQ WTW 

« Hide

References

[1]"The embryonic vertebrate forebrain: the prosomeric model."
Rubenstein J.L., Martinez S., Shimamura K., Puelles L.
Science 266:578-580(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The cDNA sequence of murine Nkx-2.2."
Hartigan D.J., Rubenstein J.L.
Gene 168:271-272(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Regional expression of the homeobox gene Nkx-2.2 in the developing mammalian forebrain."
Price M., Lazzaro D., Pohl T., Mattei M.-G., Ruether U., Olivo J.-C., Duboule D., di Lauro R.
Neuron 8:241-255(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 112-196.
[4]"Cross-repressive interaction of the Olig2 and Nkx2.2 transcription factors in developing neural tube associated with formation of a specific physical complex."
Sun T., Dong H., Wu L., Kane M., Rowitch D.H., Stiles C.D.
J. Neurosci. 23:9547-9556(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OLIG2, SUBCELLULAR LOCATION.
[5]"Cooperative transcriptional regulation of the essential pancreatic islet gene NeuroD1 (beta2) by Nkx2.2 and neurogenin 3."
Anderson K.R., Torres C.A., Solomon K., Becker T.C., Newgard C.B., Wright C.V., Hagman J., Sussel L.
J. Biol. Chem. 284:31236-31248(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ASSOCIATION WITH CHROMATIN, DNA-BINDING, MUTAGENESIS OF LYS-184.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U31566 mRNA. Translation: AAA79303.1.
S83259 Genomic DNA. No translation available.
CCDSCCDS16834.1.
PIRJC4634.
RefSeqNP_035049.1. NM_010919.2.
XP_006498961.1. XM_006498898.1.
XP_006498962.1. XM_006498899.1.
XP_006498963.1. XM_006498900.1.
UniGeneMm.330639.

3D structure databases

ProteinModelPortalP42586.
SMRP42586. Positions 127-189.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201776. 4 interactions.
IntActP42586. 1 interaction.
MINTMINT-4103861.

Proteomic databases

PaxDbP42586.
PRIDEP42586.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000067075; ENSMUSP00000069666; ENSMUSG00000027434.
GeneID18088.
KEGGmmu:18088.
UCSCuc008msu.1. mouse.

Organism-specific databases

CTD4821.
MGIMGI:97347. Nkx2-2.

Phylogenomic databases

eggNOGNOG310976.
HOGENOMHOG000231923.
HOVERGENHBG006689.
InParanoidP42586.
KOK08029.
OMAGSALCEY.
PhylomeDBP42586.
TreeFamTF351204.

Enzyme and pathway databases

ReactomeREACT_13641. Regulation of Beta-Cell Development.

Gene expression databases

BgeeP42586.
GenevestigatorP42586.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00024. HOMEOBOX.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio293225.
PROP42586.
SOURCESearch...

Entry information

Entry nameNKX22_MOUSE
AccessionPrimary (citable) accession number: P42586
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot