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P42586

- NKX22_MOUSE

UniProt

P42586 - NKX22_MOUSE

Protein

Homeobox protein Nkx-2.2

Gene

Nkx2-2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi128 – 18760HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: UniProtKB
    2. core promoter proximal region DNA binding Source: UniProtKB
    3. protein binding Source: MGI
    4. sequence-specific DNA binding Source: MGI
    5. sequence-specific DNA binding transcription factor activity Source: MGI
    6. transcription coactivator activity Source: UniProtKB
    7. transcription factor binding Source: MGI

    GO - Biological processi

    1. astrocyte differentiation Source: MGI
    2. cell development Source: MGI
    3. cell differentiation Source: MGI
    4. digestive tract development Source: MGI
    5. endocrine pancreas development Source: MGI
    6. negative regulation of neuron differentiation Source: MGI
    7. nervous system development Source: MGI
    8. neuron fate specification Source: MGI
    9. oligodendrocyte development Source: MGI
    10. optic nerve development Source: Ensembl
    11. pancreatic A cell fate commitment Source: MGI
    12. pancreatic PP cell fate commitment Source: MGI
    13. positive regulation of cell differentiation Source: MGI
    14. positive regulation of neuron differentiation Source: MGI
    15. positive regulation of oligodendrocyte differentiation Source: MGI
    16. positive regulation of sequence-specific DNA binding transcription factor activity Source: UniProtKB
    17. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    18. regulation of cell differentiation Source: MGI
    19. regulation of transcription, DNA-templated Source: MGI
    20. response to glucose Source: Ensembl
    21. response to progesterone Source: Ensembl
    22. smoothened signaling pathway Source: MGI
    23. spinal cord motor neuron differentiation Source: MGI
    24. spinal cord oligodendrocyte cell differentiation Source: MGI
    25. spinal cord oligodendrocyte cell fate specification Source: MGI
    26. transcription, DNA-templated Source: UniProtKB-KW
    27. type B pancreatic cell development Source: MGI
    28. type B pancreatic cell fate commitment Source: MGI
    29. ventral spinal cord interneuron fate determination Source: MGI

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_214529. Regulation of gene expression in beta cells.
    REACT_221082. Regulation of gene expression in endocrine-committed (NEUROG3+) progenitor cells.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homeobox protein Nkx-2.2
    Alternative name(s):
    Homeobox protein NK-2 homolog B
    Gene namesi
    Name:Nkx2-2
    Synonyms:Nkx-2.2, Nkx2b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:97347. Nkx2-2.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. intracellular Source: MGI
    2. nucleoplasm Source: Reactome
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi184 – 1841K → I: Inhibits DNA-binding activity and transcriptional activation of NEUROD1 expression. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 273273Homeobox protein Nkx-2.2PRO_0000048931Add
    BLAST

    Proteomic databases

    PaxDbiP42586.
    PRIDEiP42586.

    Expressioni

    Tissue specificityi

    Expressed in restricted areas of the developing CNS: the hindbrain and forebrain, and pancreas.

    Developmental stagei

    Expressed during embryogenesis.

    Gene expression databases

    BgeeiP42586.
    GenevestigatoriP42586.

    Interactioni

    Subunit structurei

    Interacts with OLIG2.1 Publication

    Protein-protein interaction databases

    BioGridi201776. 4 interactions.
    IntActiP42586. 1 interaction.
    MINTiMINT-4103861.

    Structurei

    3D structure databases

    ProteinModelPortaliP42586.
    SMRiP42586. Positions 127-189.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The homeodomain is essential for interaction with OLIG2.

    Sequence similaritiesi

    Belongs to the NK-2 homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG310976.
    HOGENOMiHOG000231923.
    HOVERGENiHBG006689.
    InParanoidiP42586.
    KOiK08029.
    OMAiGSALCEY.
    PhylomeDBiP42586.
    TreeFamiTF351204.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    [Graphical view]
    PRINTSiPR00024. HOMEOBOX.
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42586-1 [UniParc]FASTAAdd to Basket

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    MSLTNTKTGF SVKDILDLPD TNDEDGSVAE GPEEESEGPE PAKRAGPLGQ    50
    GALDAVQSLP LKSPFYDSSD NPYTRWLAST EGLQYSLHGL AASAPPQDSS 100
    SKSPEPSADE SPDNDKETQG GGGDAGKKRK RRVLFSKAQT YELERRFRQQ 150
    RYLSAPEREH LASLIRLTPT QVKIWFQNHR YKMKRARAEK GMEVTPLPSP 200
    RRVAVPVLVR DGKPCHALKA QDLAAATFQA GIPFSAYSAQ SLQHMQYNAQ 250
    YSSASTPQYP TAHPLVQAQQ WTW 273
    Length:273
    Mass (Da):30,126
    Last modified:October 1, 1996 - v2
    Checksum:i1F3DBB8127667F19
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31566 mRNA. Translation: AAA79303.1.
    S83259 Genomic DNA. No translation available.
    CCDSiCCDS16834.1.
    PIRiJC4634.
    RefSeqiNP_035049.1. NM_010919.2.
    XP_006498961.1. XM_006498898.1.
    XP_006498962.1. XM_006498899.1.
    XP_006498963.1. XM_006498900.1.
    UniGeneiMm.330639.

    Genome annotation databases

    EnsembliENSMUST00000067075; ENSMUSP00000069666; ENSMUSG00000027434.
    GeneIDi18088.
    KEGGimmu:18088.
    UCSCiuc008msu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31566 mRNA. Translation: AAA79303.1 .
    S83259 Genomic DNA. No translation available.
    CCDSi CCDS16834.1.
    PIRi JC4634.
    RefSeqi NP_035049.1. NM_010919.2.
    XP_006498961.1. XM_006498898.1.
    XP_006498962.1. XM_006498899.1.
    XP_006498963.1. XM_006498900.1.
    UniGenei Mm.330639.

    3D structure databases

    ProteinModelPortali P42586.
    SMRi P42586. Positions 127-189.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201776. 4 interactions.
    IntActi P42586. 1 interaction.
    MINTi MINT-4103861.

    Proteomic databases

    PaxDbi P42586.
    PRIDEi P42586.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000067075 ; ENSMUSP00000069666 ; ENSMUSG00000027434 .
    GeneIDi 18088.
    KEGGi mmu:18088.
    UCSCi uc008msu.1. mouse.

    Organism-specific databases

    CTDi 4821.
    MGIi MGI:97347. Nkx2-2.

    Phylogenomic databases

    eggNOGi NOG310976.
    HOGENOMi HOG000231923.
    HOVERGENi HBG006689.
    InParanoidi P42586.
    KOi K08029.
    OMAi GSALCEY.
    PhylomeDBi P42586.
    TreeFami TF351204.

    Enzyme and pathway databases

    Reactomei REACT_214529. Regulation of gene expression in beta cells.
    REACT_221082. Regulation of gene expression in endocrine-committed (NEUROG3+) progenitor cells.

    Miscellaneous databases

    NextBioi 293225.
    PROi P42586.
    SOURCEi Search...

    Gene expression databases

    Bgeei P42586.
    Genevestigatori P42586.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    [Graphical view ]
    PRINTSi PR00024. HOMEOBOX.
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The embryonic vertebrate forebrain: the prosomeric model."
      Rubenstein J.L., Martinez S., Shimamura K., Puelles L.
      Science 266:578-580(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Regional expression of the homeobox gene Nkx-2.2 in the developing mammalian forebrain."
      Price M., Lazzaro D., Pohl T., Mattei M.-G., Ruether U., Olivo J.-C., Duboule D., di Lauro R.
      Neuron 8:241-255(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 112-196.
    4. "Cross-repressive interaction of the Olig2 and Nkx2.2 transcription factors in developing neural tube associated with formation of a specific physical complex."
      Sun T., Dong H., Wu L., Kane M., Rowitch D.H., Stiles C.D.
      J. Neurosci. 23:9547-9556(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH OLIG2, SUBCELLULAR LOCATION.
    5. "Cooperative transcriptional regulation of the essential pancreatic islet gene NeuroD1 (beta2) by Nkx2.2 and neurogenin 3."
      Anderson K.R., Torres C.A., Solomon K., Becker T.C., Newgard C.B., Wright C.V., Hagman J., Sussel L.
      J. Biol. Chem. 284:31236-31248(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ASSOCIATION WITH CHROMATIN, DNA-BINDING, MUTAGENESIS OF LYS-184.

    Entry informationi

    Entry nameiNKX22_MOUSE
    AccessioniPrimary (citable) accession number: P42586
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3