P42573 (CED3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell death protein 3
EC=3.4.22.-

Cleaved into the following 2 chains:

1. Cell death protein 3 subunit 1
2. Cell death protein 3 subunit 2

Gene names

Name:	ced-3
ORF Names:	C48D1.2

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a cysteine protease in controlling programmed cell death by proteolytically activating or inactivating a substrate protein or proteins, a potential substrate may be ced-4. Alternatively it might directly cause cell death by proteolytically cleaving proteins that are crucial for cell viability.
Subunit structure	Could be a heterodimer of two subunits derived from the precursor sequence by a probable autocatalytic mechanism.
Developmental stage	Most abundant during embryogenesis and is also detected at later stages.
Post-translational modification	May be regulated by phosphorylation.
Sequence similarities	Belongs to the peptidase C14A family. Contains 1 CARD domain.

Ontologies

Keywords
Biological process	Apoptosis
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cleavage of cytosolic proteins involved in apoptosis Inferred from direct assay. Source: WormBase determination of adult lifespan Inferred from mutant phenotype. Source: WormBase regulation of apoptotic process Inferred from electronic annotation. Source: InterPro reproduction Inferred from mutant phenotype. Source: WormBase
Cellular component	cytoplasm Inferred from direct assay. Source: WormBase membrane Inferred from direct assay. Source: WormBase
Molecular function	cysteine-type endopeptidase activity Inferred from direct assay. Source: WormBase identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		6	EBI-494247,EBI-494247
ced-4	P30429	8	EBI-494247,EBI-494118
ced-4	P30429-2	9	EBI-494247,EBI-536271
ced-9	P41958	2	EBI-494247,EBI-494110

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	Cell death protein 3 subunit 1 Potential		PRO_0000004674
	Chain	372 – 503	132	Cell death protein 3 subunit 2 Potential		PRO_0000004675
Regions
	Domain	1 – 91	91	CARD
	Compositional bias	107 – 205	99	Ser-rich
Sites
	Active site	315	1	By similarity
	Active site	358	1	By similarity
Experimental info
	Mutagenesis	27	1	L → F in N1040; loss of function. Ref.1
	Mutagenesis	65	1	G → R in N718; loss of function. Ref.1
	Mutagenesis	360	1	G → S in N2433; loss of function. Ref.1
	Mutagenesis	449	1	A → V in N1229/N1164; loss of function. Ref.1
	Mutagenesis	466	1	A → V in N2430; loss of function. Ref.1
	Mutagenesis	483	1	E → K in N2426; loss of function. Ref.1
	Mutagenesis	486	1	S → F in N1163; loss of function. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P42573 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: 722D5831F94DAA69	FASTA	503	56,617
	10 20 30 40 50 60 MMRQDRRSLL ERNIMMFSSH LKVDEILEVL IAKQVLNSDN GDMINSCGTV REKRREIVKA 70 80 90 100 110 120 VQRRGDVAFD AFYDALRSTG HEGLAEVLEP LARSVDSNAV EFECPMSPAS HRRSRALSPA 130 140 150 160 170 180 GYTSPTRVHR DSVSSVSSFT SYQDIYSRAR SRSRSRALHS SDRHNYSSPP VNAFPSQPSS 190 200 210 220 230 240 ANSSFTGCSS LGYSSSRNRS FSKASGPTQY IFHEEDMNFV DAPTISRVFD EKTMYRNFSS 250 260 270 280 290 300 PRGMCLIINN EHFEQMPTRN GTKADKDNLT NLFRCMGYTV ICKDNLTGRG MLLTIRDFAK 310 320 330 340 350 360 HESHGDSAIL VILSHGEENV IIGVDDIPIS THEIYDLLNA ANAPRLANKP KIVFVQACRG 370 380 390 400 410 420 ERRDNGFPVL DSVDGVPAFL RRGWDNRDGP LFNFLGCVRP QVQQVWRKKP SQADILIAYA 430 440 450 460 470 480 TTAQYVSWRN SARGSWFIQA VCEVFSTHAK DMDVVELLTE VNKKVACGFQ TSQGSNILKQ 490 500 MPEMTSRLLK KFYFWPEARN SAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme." Yuan J., Shaham S., Ledoux S., Ellis H.M., Horvitz H.R. Cell 75:641-652(1993) [PubMed: 8242740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF LEU-27; GLY-65; GLY-360; ALA-449; ALA-466; GLU-483 AND SER-486. Strain: Bristol N2.
[2]	Horvitz H.R. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 418.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L29052 Genomic DNA. Translation: AAA27982.2. AF210702 mRNA. Translation: AAG42045.1. Z81049 Genomic DNA. Translation: CAB61001.2.
PIR	A49429.
RefSeq	NP_502538.1. NM_070137.4.
UniGene	Cel.19438.
3D structure databases
ProteinModelPortal	P42573.
SMR	P42573. Positions 1-92, 231-497.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-244N.
IntAct	P42573. 5 interactions.
MINT	MINT-128934.
STRING	P42573.
Protein family/group databases
MEROPS	C14.002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C48D1.2a; C48D1.2a; C48D1.2.
GeneID	178272.
KEGG	cel:C48D1.2.
NMPDR	fig|6239.3.peg.15680.
UCSC	C48D1.2. c. elegans.
Organism-specific databases
CTD	178272.
WormBase	C48D1.2a; CE29088; WBGene00000417; ced-3.
Phylogenomic databases
eggNOG	meNOG09091.
GeneTree	EMGT00050000010307.
HOGENOM	HBG383757.
InParanoid	P42573.
OMA	YLSAIVY.
PhylomeDB	P42573.
Gene expression databases
ArrayExpress	P42573.
Family and domain databases
InterPro	IPR001315. CARD. IPR011029. DEATH-like. IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view]
Gene3D	G3DSA:1.10.533.10. DEATH_like. 1 hit.
KO	K04489.
PANTHER	PTHR10454. Pept_C14_p45. 1 hit.
Pfam	PF00619. CARD. 1 hit. PF00656. Peptidase_C14. 1 hit. [Graphical view]
PRINTS	PR00376. IL1BCENZYME.
SMART	SM00114. CARD. 1 hit. SM00115. CASc. 1 hit. [Graphical view]
SUPFAM	SSF47986. DEATH_like. 1 hit.
PROSITE	PS50209. CARD. 1 hit. PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. 1 hit. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	900454.

Entry information

Entry name

CED3_CAEEL

Accession

Primary (citable) accession number: P42573
Secondary accession number(s): P45435, Q9GQQ4, Q9NAQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	August 14, 2001
Last modified:	November 16, 2011
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project