Reviewed, UniProtKB/Swiss-Prot P42535 (PCPB_SPHCR)

Names and origin

Protein names

Recommended name:
    Pentachlorophenol 4-monooxygenase
    EC=1.14.13.50
Alternative name(s):
    Pentachlorophenol hydroxylase

Gene names

Name:

pcpB

Organism

Sphingobium chlorophenolicum

Taxonomic identifier

46429 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingobium

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol. Ref.2
Catalytic activity	Pentachlorophenol + 2 NADPH + O2 = 2,3,5,6-tetrachlorohydroquinone + 2 NADP+ + chloride + H2O. 2,3,5,6-tetrachlorophenol + NADPH + O2 = 2,3,5,6-tetrachlorohydroquinone + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Xenobiotic degradation; pentachlorophenol degradation.
Induction	By polychlorinated phenols. Ref.2
Sequence similarities	Belongs to the pheA/tfdB FAD monooxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	pentachlorophenol monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 538	537	Pentachlorophenol 4-monooxygenase		PRO_0000214044
Regions
	Nucleotide binding	16 – 45	30	FAD Potential
	Nucleotide binding	288 – 298	11	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P42535-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: CB545A9A1DCE8AB9	FASTA	538	59,992
	10 20 30 40 50 60 MSTYPINAPG QSADAAVLIV GGGPTGLIAA NELLRRGVSC RMIDRLPVAH QTSKSCTIHA 70 80 90 100 110 120 RSMEMMEHIG IAARYIETGV RSNGFTFNFE NTDANALLDF SVLPGRYPFI TIYNQNETER 130 140 150 160 170 180 VLRHDLEATY SFQPEWGTQL LALNQDENGI RADLRLKDGT KQTISPRWVI GADGVRSRVR 190 200 210 220 230 240 ECLGIAYEGE DYEENVLQMM DVGIQDFEAG DDWIHYFIGQ DKFVFVTKLP GSNYRVIISD 250 260 270 280 290 300 LGGANKSNLE ETREAFQGYL SSFDDHATLD EPRWATKWRV WKRMATAYRK GNVFLAGDAA 310 320 330 340 350 360 HCHSPSGGSG MNVGMQDAFN LGWKIAMVER GEAKPDLLDT YHTERTPVAQ QLLEGTHAMH 370 380 390 400 410 420 EIIMGHGKGL TDRIELTQAP GWHDAATYRV SGMSYNYRDQ LVSFNDDRLA GPSAGDRIPD 430 440 450 460 470 480 AELAPRIRLF DLVRNTRPTL LVAPATEAEV AEAEKLRDLI REQWPLVKPV LVRPQGSEES 490 500 510 520 530 IEGDVHVDSY GQLKREWGDN AKGWAALLRP DNYIHARAGL DRGDLLVQAI DAMLVRCA

References

[1]	"Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in Escherichia coli." Orser C.S., Lange C.C., Xun L., Zahrt T.C., Schneider B.J. J. Bacteriol. 175:411-416(1993) [PubMed: 7678243] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-14. Strain: ATCC 39723 / DSM 6824 / L-1.
[2]	"Organization and regulation of pentachlorophenol-degrading genes in Sphingobium chlorophenolicum ATCC 39723." Cai M., Xun L. J. Bacteriol. 184:4672-4680(2002) [PubMed: 12169590] [Abstract] Cited for: SEQUENCE REVISION TO 536, FUNCTION, INDUCTION. Strain: ATCC 39723 / DSM 6824 / L-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U12290 Genomic DNA. Translation: AAF15368.2.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR002938. mOase_FAD_bd. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01494. FAD_binding_3. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

PCPB_SPHCR

Accession

Primary (citable) accession number: P42535
Secondary accession number(s): Q8KMS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	March 24, 2009
This is version 58 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)