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Protein

Myosin heavy chain kinase A

Gene

mhkA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates threonine in the C-terminal tail region of myosin II heavy chain. This phosphorylation is critical in regulating the assembly and disassembly of myosin II filament. Requires autophosphorylation for activity.

Catalytic activityi

ATP + [myosin heavy-chain] = ADP + [myosin heavy-chain] phosphate.

Cofactori

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi778 – 783ATPSequence analysis6

GO - Molecular functioni

  • actin filament binding Source: dictyBase
  • ATPase activity Source: dictyBase
  • ATP binding Source: dictyBase
  • myosin heavy chain kinase activity Source: dictyBase
  • myosin II binding Source: dictyBase
  • protein kinase activity Source: dictyBase
  • protein serine/threonine kinase activity Source: dictyBase

GO - Biological processi

  • actin crosslink formation Source: dictyBase
  • actin filament bundle assembly Source: dictyBase
  • chemotaxis Source: dictyBase
  • mitotic cytokinesis Source: dictyBase
  • myosin II filament disassembly Source: dictyBase
  • peptidyl-threonine phosphorylation Source: dictyBase
  • protein autophosphorylation Source: dictyBase
  • protein homooligomerization Source: dictyBase
  • protein phosphorylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.7. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin heavy chain kinase A (EC:2.7.11.7)
Short name:
MHCK-A
Gene namesi
Name:mhkA
Synonyms:mhckA
ORF Names:DDB_G0291231
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291231. mhkA.

Subcellular locationi

GO - Cellular componenti

  • actomyosin contractile ring Source: GO_Central
  • cell cortex Source: dictyBase
  • cytoplasm Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510471 – 1146Myosin heavy chain kinase AAdd BLAST1146

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP42527.

Interactioni

Subunit structurei

Oligomer.

GO - Molecular functioni

  • actin filament binding Source: dictyBase
  • myosin II binding Source: dictyBase

Protein-protein interaction databases

STRINGi44689.DDB0216274.

Structurei

Secondary structure

11146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi554 – 556Combined sources3
Beta strandi558 – 566Combined sources9
Turni567 – 570Combined sources4
Beta strandi571 – 581Combined sources11
Beta strandi586 – 588Combined sources3
Beta strandi590 – 605Combined sources16
Beta strandi614 – 618Combined sources5
Helixi620 – 622Combined sources3
Beta strandi623 – 625Combined sources3
Helixi632 – 635Combined sources4
Beta strandi641 – 647Combined sources7
Beta strandi649 – 651Combined sources3
Helixi652 – 654Combined sources3
Helixi657 – 679Combined sources23
Beta strandi692 – 695Combined sources4
Beta strandi703 – 705Combined sources3
Beta strandi709 – 714Combined sources6
Beta strandi720 – 722Combined sources3
Beta strandi726 – 728Combined sources3
Helixi735 – 747Combined sources13
Turni748 – 750Combined sources3
Beta strandi753 – 755Combined sources3
Beta strandi758 – 760Combined sources3
Beta strandi763 – 765Combined sources3
Beta strandi768 – 770Combined sources3
Helixi783 – 793Combined sources11
Helixi798 – 802Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LKMX-ray1.60A552-841[»]
3LLAX-ray2.11A/B552-841[»]
3LMHX-ray2.00A/B552-841[»]
3LMIX-ray2.20A/B/C/D552-841[»]
3PDTX-ray1.80A552-809[»]
4ZMEX-ray1.98A/B552-841[»]
4ZMFX-ray2.39A/B552-841[»]
4ZS4X-ray2.40A/B552-841[»]
5DYJX-ray2.50A/B552-841[»]
5E4HX-ray2.90A/B/C/D/E/F/G/H552-841[»]
5E9EX-ray2.40A/B552-841[»]
ProteinModelPortaliP42527.
SMRiP42527.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42527.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini564 – 808Alpha-type protein kinasePROSITE-ProRule annotationAdd BLAST245
Repeati867 – 897WD 1Add BLAST31
Repeati910 – 938WD 2Add BLAST29
Repeati952 – 980WD 3Add BLAST29
Repeati993 – 1021WD 4Add BLAST29
Repeati1033 – 1061WD 5Add BLAST29
Repeati1073 – 1101WD 6Add BLAST29
Repeati1114 – 1142WD 7Add BLAST29

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni500 – 551Pseudosubstrate/autoinhibitory domainSequence analysisAdd BLAST52
Regioni552 – 852CatalyticAdd BLAST301

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili100 – 120Sequence analysisAdd BLAST21
Coiled coili187 – 241Sequence analysisAdd BLAST55
Coiled coili297 – 502Sequence analysisAdd BLAST206

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi144 – 148Poly-Gln5
Compositional biasi175 – 181Poly-Gly7
Compositional biasi345 – 348Poly-Ser4
Compositional biasi438 – 441Poly-Leu4

Sequence similaritiesi

Contains 1 alpha-type protein kinase domain.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0274. Eukaryota.
ENOG410XRWX. LUCA.
InParanoidiP42527.
OMAiGNEQVER.
PhylomeDBiP42527.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02816. Alpha_kinase. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00811. Alpha_kinase. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51158. ALPHA_KINASE. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNIKKRKES ITGIPPINVN SPQSVPLSGT LQSPLITPNS PNFVSRQCPF
60 70 80 90 100
KKFGCSSFLV SKAEFDNHLK DDAQFHLQLA VEKFDHQFDL HTQLMAHFTE
110 120 130 140 150
QMEDQLEKTM KVVRNHTDSL GGNVQTKLDE GIEKCMAFAK KVEQQQQQLA
160 170 180 190 200
KRLITQQIQE KKSTSSPLVK GGISGGGGSG GDDSFDGANI SSMSTSKQEL
210 220 230 240 250
QQELQSLSIK MKKELTELSD ELSQKLERST GNIDIKIKRI EGEVNEKIDK
260 270 280 290 300
RQLVSTIDDS IGKKTDSIGY TLESSIIKKV EEKEKKKSEQ NQLLFDSKIE
310 320 330 340 350
SLKDKIKIIE TQQLDTSSEV RKLKLESTSS GNLMAGLNGT SGRPSSSSHF
360 370 380 390 400
IPSSVSAAAN NINKNEIMEE VKKVEEKLQK KIREEIDNTK SELSKVERSV
410 420 430 440 450
KDNRSEIEGL EKDCKNQFDK QDNKIKQVED DLKKSDSLLL LMQNNLKKYN
460 470 480 490 500
EFVDRERDRE SERLKLQDSI KRLEQNQKKI EAEIQEGNEQ VERVLREEAS
510 520 530 540 550
ISPISSVPKS PITTKRSSII LNSPPMTSQQ SSPKIQDLLS SSGSSSVSGI
560 570 580 590 600
NISSETGEMG ILWEFDPIIN KWIRLSMKLK VERKPFAEGA LREAYHTVSL
610 620 630 640 650
GVGTDENYPL GTTTKLFPPI EMISPISKNN EAMTQLKNGT KFVLKLYKKE
660 670 680 690 700
AEQQASRELY FEDVKMQMVC RDWGNKFNQK KPPKKIEFLM SWVVELIDRS
710 720 730 740 750
PSSNGQPILC SIEPLLVGEF KKNNSNYGAV LTNRSTPQAF SHFTYELSNK
760 770 780 790 800
QMIVVDIQGV DDLYTDPQIH TPDGKGFGLG NLGKAGINKF ITTHKCNAVC
810 820 830 840 850
ALLDLDVKLG GVLSGNNKKQ LQQGTMVMPD ILPELMPSDN TIKVGAKQLP
860 870 880 890 900
KAEFSKKDLK CVSTIQSFRE RVNSIAFFDN QKLLCAGYGD GTYRVFDVND
910 920 930 940 950
NWKCLYTVNG HRKSIESIAC NSNYIFTSSP DNTIKVHIIR SGNTKCIETL
960 970 980 990 1000
VGHTGEVNCV VANEKYLFSC SYDKTIKVWD LSTFKEIKSF EGVHTKYIKT
1010 1020 1030 1040 1050
LALSGRYLFS GGNDQIIYVW DTETLSMLFN MQGHEDWVLS LHCTASYLFS
1060 1070 1080 1090 1100
TSKDNVIKIW DLSNFSCIDT LKGHWNSVSS CVVKDRYLYS GSEDNSIKVW
1110 1120 1130 1140
DLDTLECVYT IPKSHSLGVK CLMVFNNQII SAAFDGSIKV WEWQSK
Length:1,146
Mass (Da):128,962
Last modified:December 4, 2007 - v2
Checksum:i54A651F6CC38CA8E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti391S → A in AAA66070 (PubMed:7822274).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16856 mRNA. Translation: AAA66070.1.
AAFI02000177 Genomic DNA. Translation: EAL61599.1.
PIRiA55532.
RefSeqiXP_635119.1. XM_630027.1.

Genome annotation databases

EnsemblProtistsiEAL61599; EAL61599; DDB_G0291231.
GeneIDi8628066.
KEGGiddi:DDB_G0291231.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16856 mRNA. Translation: AAA66070.1.
AAFI02000177 Genomic DNA. Translation: EAL61599.1.
PIRiA55532.
RefSeqiXP_635119.1. XM_630027.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LKMX-ray1.60A552-841[»]
3LLAX-ray2.11A/B552-841[»]
3LMHX-ray2.00A/B552-841[»]
3LMIX-ray2.20A/B/C/D552-841[»]
3PDTX-ray1.80A552-809[»]
4ZMEX-ray1.98A/B552-841[»]
4ZMFX-ray2.39A/B552-841[»]
4ZS4X-ray2.40A/B552-841[»]
5DYJX-ray2.50A/B552-841[»]
5E4HX-ray2.90A/B/C/D/E/F/G/H552-841[»]
5E9EX-ray2.40A/B552-841[»]
ProteinModelPortaliP42527.
SMRiP42527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0216274.

Proteomic databases

PaxDbiP42527.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL61599; EAL61599; DDB_G0291231.
GeneIDi8628066.
KEGGiddi:DDB_G0291231.

Organism-specific databases

dictyBaseiDDB_G0291231. mhkA.

Phylogenomic databases

eggNOGiKOG0274. Eukaryota.
ENOG410XRWX. LUCA.
InParanoidiP42527.
OMAiGNEQVER.
PhylomeDBiP42527.

Enzyme and pathway databases

BRENDAi2.7.11.7. 1939.

Miscellaneous databases

EvolutionaryTraceiP42527.
PROiP42527.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR011009. Kinase-like_dom.
IPR004166. MHCK_EF2_kinase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF02816. Alpha_kinase. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00811. Alpha_kinase. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51158. ALPHA_KINASE. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMHCKA_DICDI
AccessioniPrimary (citable) accession number: P42527
Secondary accession number(s): Q54EX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.