Reviewed, UniProtKB/Swiss-Prot P42525 (ERK1_DICDI)

Names and origin

Protein names

Recommended name:
    Extracellular signal-regulated kinase 1
      Short name=ERK1
    EC=2.7.11.24
Alternative name(s):
    MAP kinase 1

Gene names

Name:	erkA
Synonyms:	erk1
ORF Names:	DDB_G0286353

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Kinase involved in a signal transduction pathway. Ref.2
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases By similarity.
Post-translational modification	Dually phosphorylated on Thr-309 and Tyr-311, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Extracellular signal-regulated kinase 1		PRO_0000186308
Regions
	Domain	149 – 439	291	Protein kinase
	Nucleotide binding	155 – 163	9	ATP By similarity
	Motif	309 – 311	3	TXY
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	178	1	ATP By similarity
Amino acid modifications
	Modified residue	309	1	Phosphothreonine By similarity
	Modified residue	311	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	389	1	R → RSL in AAA59387. Ref.1
	Sequence conflict	517	1	Q → P in AAA59387. Ref.1
	Sequence conflict	529	1	N → INN in AAA59387. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P42525-1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 495C8ACD0215A3A7	FASTA	529	61,303
	10 20 30 40 50 60 MEPEFDHFQS QMDSDNTHQS TMFNVQDNNA ILMSGMENVL QSPRQLQAAA QAQQQAAAQA 70 80 90 100 110 120 QQQQVQAQQV QAQQAQQQQQ QQQNQQQQQQ QQNQQNQQQQ QNQQQQSQQM TQQQLQQLMP 130 140 150 160 170 180 PPPTSDTSNF NDNISYFVYG SQFTVPRRYS IVKCIGHGAY GVVCSAKDNL TGEKVAIKKI 190 200 210 220 230 240 SKAFDNLKDT KRTLREIHLL RHFKHENLIS IKDILKPNSK EQFEDVYIVS ELMDTDLHQI 250 260 270 280 290 300 ITSPQPLSDD HCQYFVYQML RGLKHIHSAN VLHRDLKPSN LLINEDCLLK ICDLGLARVE 310 320 330 340 350 360 DATHQGFMTE YVATRWYRAP EVILSWNKYT KAIDIWSVGC IFAELLGRKP LFQGKDYIHQ 370 380 390 400 410 420 ITLIIETIGS PSEEDICNIA NEQARQFIRN MGNQPKVNFA NMFPKANPDA IDLLERMLYF 430 440 450 460 470 480 DPSKRLTVEE ALAHPYFQSL HDPSDEPICL HKFSLNFEAW DLNRDLLKEL IYNEMLAYHP 490 500 510 520 EDPQAPYYTD LNNPNFNLSR IQSSSELFNL LQQQKQQIHQ QVNQQSIKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Identification and functional analysis of a developmentally regulated extracellular signal-regulated kinase gene in Dictyostelium discoideum." Gaskins C.J., Maeda M., Firtel R.A. Mol. Cell. Biol. 14:6996-7012(1994) [PubMed: 7935416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 96-529, FUNCTION. Strain: AX3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000085 Genomic DNA. Translation: EAL64201.1. U11077 mRNA. Translation: AAA59387.1. Different initiation.
PIR	A56042.
RefSeq	XP_637704.1.
3D structure databases
HSSP	HSSP built from PDB template 1KV1 based on UniProtKB Q16539.
ModBase	Search...
Genome annotation databases
GeneID	3389341.
GenomeReviews	Gene locus erkA in contig CM000153_GR.
KEGG	ddi:DDB_0201635.
Organism-specific databases
dictyBase	DDB_G0286353. erkA.
Phylogenomic databases
OMA	ENIGIGA.
Enzyme and pathway databases
BRENDA	2.7.11.24. 424.
Family and domain databases
InterPro	IPR003527. MAP_kinase_CS. IPR008352. MAPK_p38. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01773. P38MAPKINASE.
ProDom	PD000001. Prot_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 2 hits. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERK1_DICDI

Accession

Primary (citable) accession number: P42525
Secondary accession number(s): Q54LX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)