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P42525

- ERK1_DICDI

UniProt

P42525 - ERK1_DICDI

Protein

Extracellular signal-regulated kinase 1

Gene

erkA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Kinase involved in a signal transduction pathway.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by tyrosine and threonine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei178 – 1781ATPPROSITE-ProRule annotation
    Active sitei275 – 2751Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi155 – 1639ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC
    3. protein kinase activity Source: dictyBase

    GO - Biological processi

    1. aggregation involved in sorocarp development Source: dictyBase
    2. chemotaxis to cAMP Source: dictyBase
    3. MAPK cascade Source: dictyBase
    4. mitotic nuclear division Source: UniProtKB-KW
    5. protein phosphorylation Source: dictyBase
    6. sorocarp development Source: dictyBase

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 1939.
    ReactomeiREACT_204254. NOD1/2 Signaling Pathway.
    REACT_206814. Signaling by FGFR.
    REACT_208820. activated TAK1 mediates p38 MAPK activation.
    REACT_209442. ERK1 activation.
    REACT_209920. CDO in myogenesis.
    REACT_210783. p38MAPK events.
    REACT_214343. Oxidative Stress Induced Senescence.
    REACT_220482. ADP signalling through P2Y purinoceptor 1.
    REACT_220796. Signal transduction by L1.
    REACT_221153. ERK2 activation.
    REACT_221807. ERK/MAPK targets.
    REACT_226365. Signalling to ERK5.
    REACT_227492. Regulation of HSF1-mediated heat shock response.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Extracellular signal-regulated kinase 1 (EC:2.7.11.24)
    Short name:
    ERK1
    Alternative name(s):
    MAP kinase 1
    Gene namesi
    Name:erkA
    Synonyms:erk1
    ORF Names:DDB_G0286353
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0286353. erkA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 529529Extracellular signal-regulated kinase 1PRO_0000186308Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei309 – 3091PhosphothreonineBy similarity
    Modified residuei311 – 3111PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-309 and Tyr-311, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0201635.

    Structurei

    3D structure databases

    ProteinModelPortaliP42525.
    SMRiP42525. Positions 132-481.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini149 – 439291Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi309 – 3113TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.By similarity

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    KOiK04371.
    OMAiTIDEFHA.
    PhylomeDBiP42525.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01773. P38MAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42525-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPEFDHFQS QMDSDNTHQS TMFNVQDNNA ILMSGMENVL QSPRQLQAAA    50
    QAQQQAAAQA QQQQVQAQQV QAQQAQQQQQ QQQNQQQQQQ QQNQQNQQQQ 100
    QNQQQQSQQM TQQQLQQLMP PPPTSDTSNF NDNISYFVYG SQFTVPRRYS 150
    IVKCIGHGAY GVVCSAKDNL TGEKVAIKKI SKAFDNLKDT KRTLREIHLL 200
    RHFKHENLIS IKDILKPNSK EQFEDVYIVS ELMDTDLHQI ITSPQPLSDD 250
    HCQYFVYQML RGLKHIHSAN VLHRDLKPSN LLINEDCLLK ICDLGLARVE 300
    DATHQGFMTE YVATRWYRAP EVILSWNKYT KAIDIWSVGC IFAELLGRKP 350
    LFQGKDYIHQ ITLIIETIGS PSEEDICNIA NEQARQFIRN MGNQPKVNFA 400
    NMFPKANPDA IDLLERMLYF DPSKRLTVEE ALAHPYFQSL HDPSDEPICL 450
    HKFSLNFEAW DLNRDLLKEL IYNEMLAYHP EDPQAPYYTD LNNPNFNLSR 500
    IQSSSELFNL LQQQKQQIHQ QVNQQSIKN 529
    Length:529
    Mass (Da):61,303
    Last modified:December 4, 2007 - v2
    Checksum:i495C8ACD0215A3A7
    GO

    Sequence cautioni

    The sequence AAA59387.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti389 – 3891R → RSL in AAA59387. (PubMed:15875012)Curated
    Sequence conflicti517 – 5171Q → P in AAA59387. (PubMed:15875012)Curated
    Sequence conflicti529 – 5291N → INN in AAA59387. (PubMed:15875012)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000085 Genomic DNA. Translation: EAL64201.1.
    U11077 mRNA. Translation: AAA59387.1. Different initiation.
    PIRiA56042.
    RefSeqiXP_637704.1. XM_632612.1.

    Genome annotation databases

    EnsemblProtistsiDDB0201635; DDB0201635; DDB_G0286353.
    GeneIDi8625569.
    KEGGiddi:DDB_G0286353.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000085 Genomic DNA. Translation: EAL64201.1 .
    U11077 mRNA. Translation: AAA59387.1 . Different initiation.
    PIRi A56042.
    RefSeqi XP_637704.1. XM_632612.1.

    3D structure databases

    ProteinModelPortali P42525.
    SMRi P42525. Positions 132-481.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0201635.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0201635 ; DDB0201635 ; DDB_G0286353 .
    GeneIDi 8625569.
    KEGGi ddi:DDB_G0286353.

    Organism-specific databases

    dictyBasei DDB_G0286353. erkA.

    Phylogenomic databases

    eggNOGi COG0515.
    KOi K04371.
    OMAi TIDEFHA.
    PhylomeDBi P42525.

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 1939.
    Reactomei REACT_204254. NOD1/2 Signaling Pathway.
    REACT_206814. Signaling by FGFR.
    REACT_208820. activated TAK1 mediates p38 MAPK activation.
    REACT_209442. ERK1 activation.
    REACT_209920. CDO in myogenesis.
    REACT_210783. p38MAPK events.
    REACT_214343. Oxidative Stress Induced Senescence.
    REACT_220482. ADP signalling through P2Y purinoceptor 1.
    REACT_220796. Signal transduction by L1.
    REACT_221153. ERK2 activation.
    REACT_221807. ERK/MAPK targets.
    REACT_226365. Signalling to ERK5.
    REACT_227492. Regulation of HSF1-mediated heat shock response.

    Miscellaneous databases

    PROi P42525.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01773. P38MAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Identification and functional analysis of a developmentally regulated extracellular signal-regulated kinase gene in Dictyostelium discoideum."
      Gaskins C.J., Maeda M., Firtel R.A.
      Mol. Cell. Biol. 14:6996-7012(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 96-529, FUNCTION.
      Strain: AX3.

    Entry informationi

    Entry nameiERK1_DICDI
    AccessioniPrimary (citable) accession number: P42525
    Secondary accession number(s): Q54LX6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3