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Protein

Extracellular signal-regulated kinase 1

Gene

erkA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Kinase involved in a signal transduction pathway.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei178ATPPROSITE-ProRule annotation1
Active sitei275Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi155 – 163ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: dictyBase

GO - Biological processi

  • aggregation involved in sorocarp development Source: dictyBase
  • asexual reproduction Source: dictyBase
  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cellular response to organic substance Source: GO_Central
  • chemotaxis to cAMP Source: dictyBase
  • defense response to bacterium Source: dictyBase
  • G-protein coupled receptor signaling pathway Source: dictyBase
  • MAPK cascade Source: dictyBase
  • protein phosphorylation Source: dictyBase
  • regulation of aggregate size involved in sorocarp development Source: dictyBase
  • regulation of gene expression Source: GO_Central
  • slug development involved in sorocarp development Source: dictyBase
  • sorocarp development Source: dictyBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24 1939
ReactomeiR-DDI-110056 MAPK3 (ERK1) activation
R-DDI-112409 RAF-independent MAPK1/3 activation
R-DDI-112411 MAPK1 (ERK2) activation
R-DDI-198753 ERK/MAPK targets
R-DDI-198765 Signalling to ERK5
R-DDI-202670 ERKs are inactivated
R-DDI-2029482 Regulation of actin dynamics for phagocytic cup formation
R-DDI-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-DDI-3371453 Regulation of HSF1-mediated heat shock response
R-DDI-375165 NCAM signaling for neurite out-growth
R-DDI-442742 CREB phosphorylation through the activation of Ras
R-DDI-444257 RSK activation
R-DDI-445144 Signal transduction by L1
R-DDI-5663213 RHO GTPases Activate WASPs and WAVEs
R-DDI-5673001 RAF/MAP kinase cascade
R-DDI-5674135 MAP2K and MAPK activation
R-DDI-5674499 Negative feedback regulation of MAPK pathway
R-DDI-6798695 Neutrophil degranulation
R-DDI-881907 Gastrin-CREB signalling pathway via PKC and MAPK

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular signal-regulated kinase 1 (EC:2.7.11.24)
Short name:
ERK1
Alternative name(s):
MAP kinase 1
Gene namesi
Name:erkA
Synonyms:erk1
ORF Names:DDB_G0286353
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0286353 erkA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: dictyBase
  • nucleus Source: dictyBase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863081 – 529Extracellular signal-regulated kinase 1Add BLAST529

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei309PhosphothreonineBy similarity1
Modified residuei311PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-309 and Tyr-311, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP42525

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0201635

Structurei

3D structure databases

ProteinModelPortaliP42525
SMRiP42525
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini149 – 439Protein kinasePROSITE-ProRule annotationAdd BLAST291

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi309 – 311TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
InParanoidiP42525
KOiK04371
OMAiEWKQHIY
PhylomeDBiP42525

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008352 MAPK_p38
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01773 P38MAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P42525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPEFDHFQS QMDSDNTHQS TMFNVQDNNA ILMSGMENVL QSPRQLQAAA
60 70 80 90 100
QAQQQAAAQA QQQQVQAQQV QAQQAQQQQQ QQQNQQQQQQ QQNQQNQQQQ
110 120 130 140 150
QNQQQQSQQM TQQQLQQLMP PPPTSDTSNF NDNISYFVYG SQFTVPRRYS
160 170 180 190 200
IVKCIGHGAY GVVCSAKDNL TGEKVAIKKI SKAFDNLKDT KRTLREIHLL
210 220 230 240 250
RHFKHENLIS IKDILKPNSK EQFEDVYIVS ELMDTDLHQI ITSPQPLSDD
260 270 280 290 300
HCQYFVYQML RGLKHIHSAN VLHRDLKPSN LLINEDCLLK ICDLGLARVE
310 320 330 340 350
DATHQGFMTE YVATRWYRAP EVILSWNKYT KAIDIWSVGC IFAELLGRKP
360 370 380 390 400
LFQGKDYIHQ ITLIIETIGS PSEEDICNIA NEQARQFIRN MGNQPKVNFA
410 420 430 440 450
NMFPKANPDA IDLLERMLYF DPSKRLTVEE ALAHPYFQSL HDPSDEPICL
460 470 480 490 500
HKFSLNFEAW DLNRDLLKEL IYNEMLAYHP EDPQAPYYTD LNNPNFNLSR
510 520
IQSSSELFNL LQQQKQQIHQ QVNQQSIKN
Length:529
Mass (Da):61,303
Last modified:December 4, 2007 - v2
Checksum:i495C8ACD0215A3A7
GO

Sequence cautioni

The sequence AAA59387 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti389R → RSL in AAA59387 (PubMed:15875012).Curated1
Sequence conflicti517Q → P in AAA59387 (PubMed:15875012).Curated1
Sequence conflicti529N → INN in AAA59387 (PubMed:15875012).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000085 Genomic DNA Translation: EAL64201.1
U11077 mRNA Translation: AAA59387.1 Different initiation.
PIRiA56042
RefSeqiXP_637704.1, XM_632612.1

Genome annotation databases

EnsemblProtistsiEAL64201; EAL64201; DDB_G0286353
GeneIDi8625569
KEGGiddi:DDB_G0286353

Similar proteinsi

Entry informationi

Entry nameiERK1_DICDI
AccessioniPrimary (citable) accession number: P42525
Secondary accession number(s): Q54LX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 4, 2007
Last modified: March 28, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health