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Protein

Ribonucleoside-diphosphate reductase small subunit

Gene

rnrB-1

more
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Caution

The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.Curated

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi81Iron 1PROSITE-ProRule annotation1
Metal bindingi112Iron 1PROSITE-ProRule annotation1
Metal bindingi112Iron 2By similarity1
Metal bindingi115Iron 1PROSITE-ProRule annotation1
Active sitei119PROSITE-ProRule annotation1
Metal bindingi174Iron 2By similarity1
Metal bindingi208Iron 2By similarity1
Metal bindingi211Iron 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • deoxyribonucleotide biosynthetic process Source: InterPro
  • DNA replication Source: UniProtKB-UniPathway
  • sorocarp morphogenesis Source: dictyBase

Keywordsi

Molecular functionOxidoreductase
Biological processDNA replication
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-DDI-499943 Interconversion of nucleotide di- and triphosphates
UniPathwayiUPA00326

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Name:rnrB-1
Synonyms:nrdB
ORF Names:DDB_G0272616
AND
Name:rnrB-2
Synonyms:nrdB
ORF Names:DDB_G0274021
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0272616 rnrB-1
DDB_G0274021 rnrB-2

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: dictyBase
  • nucleus Source: dictyBase
  • ribonucleoside-diphosphate reductase complex Source: dictyBase

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001904561 – 338Ribonucleoside-diphosphate reductase small subunitAdd BLAST338

Proteomic databases

PaxDbiP42521
PRIDEiP42521

Expressioni

Developmental stagei

Expression is detected in growing cells and decreases dramatically at the onset of development. The rnrB transcript reappears after the cells have formed multicellular aggregates.1 Publication

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0238222

Structurei

3D structure databases

ProteinModelPortaliP42521
SMRiP42521
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1567 Eukaryota
COG0208 LUCA
InParanoidiP42521
KOiK10808
OMAiFYVGFVQ
PhylomeDBiP42521

Family and domain databases

CDDicd01049 RNRR2, 1 hit
Gene3Di1.10.620.20, 1 hit
InterProiView protein in InterPro
IPR009078 Ferritin-like_SF
IPR012348 RNR-like
IPR033909 RNR_small
IPR030475 RNR_small_AS
IPR000358 RNR_small_fam
PANTHERiPTHR23409 PTHR23409, 1 hit
PfamiView protein in Pfam
PF00268 Ribonuc_red_sm, 1 hit
PIRSFiPIRSF000355 NrdB, 1 hit
SUPFAMiSSF47240 SSF47240, 1 hit
PROSITEiView protein in PROSITE
PS00368 RIBORED_SMALL, 1 hit

Sequencei

Sequence statusi: Complete.

P42521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEINKKDTF IEPILKENKD RFVLFPIKYP DIWRMYKKAL ASHWVAEEID
60 70 80 90 100
LGNDNVDWEY KLTDNERHFI SHVLAFFAAS DGIVNENLAT RFMSEVQIPE
110 120 130 140 150
ARCFYGFQIA IENIHSETYS LLIETYIKDK QTKDKLFNAI ETIPCIKKKA
160 170 180 190 200
EWALRWINDS DSFAERLVAF AAVEGIFFSG SFCSIFWLKK RGLMQGLTFS
210 220 230 240 250
NELISRDEGL HCDFACLLYT KLQRKLDPKV IEKMIRDAVE CEKEFICESL
260 270 280 290 300
PVDLIGMNSR SMSQYIEFCA DRLVVSLGYK KIFNSSNPFE WMEMISLQRK
310 320 330
SNFFEGKVAE YAKTGVAIQG NNQQKNNQSR TLVLDEDF
Length:338
Mass (Da):39,371
Last modified:January 16, 2004 - v2
Checksum:i9BF33B855246CD3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36941 Genomic DNA Translation: AAB72227.2
AAFI02000011 Genomic DNA Translation: EAL70444.1
AAFI02000009 Genomic DNA Translation: EAL70945.1
RefSeqiXP_644369.1, XM_639277.1
XP_645045.1, XM_639953.1

Genome annotation databases

EnsemblProtistsiEAL70444; EAL70444; DDB_G0274021
EAL70945; EAL70945; DDB_G0272616
GeneIDi8618721
8619255
KEGGiddi:DDB_G0272616
ddi:DDB_G0274021

Similar proteinsi

Entry informationi

Entry nameiRIR2_DICDI
AccessioniPrimary (citable) accession number: P42521
Secondary accession number(s): Q556I9, Q86AN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 16, 2004
Last modified: April 25, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health