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P42521

- RIR2_DICDI

UniProt

P42521 - RIR2_DICDI

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Protein

Ribonucleoside-diphosphate reductase small subunit

Gene

rnrB-1

more
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi81 – 811Iron 1PROSITE-ProRule annotation
Metal bindingi112 – 1121Iron 1PROSITE-ProRule annotation
Metal bindingi112 – 1121Iron 2By similarity
Metal bindingi115 – 1151Iron 1PROSITE-ProRule annotation
Active sitei119 – 1191PROSITE-ProRule annotation
Metal bindingi174 – 1741Iron 2By similarity
Metal bindingi208 – 2081Iron 2By similarity
Metal bindingi211 – 2111Iron 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. DNA replication Source: dictyBase
  3. response to drug Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Name:rnrB-1
Synonyms:nrdB
ORF Names:DDB_G0272616
AND
Name:rnrB-2
Synonyms:nrdB
ORF Names:DDB_G0274021
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0272616. rnrB-1.
DDB_G0274021. rnrB-2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: dictyBase
  2. nucleus Source: dictyBase
  3. ribonucleoside-diphosphate reductase complex Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338Ribonucleoside-diphosphate reductase small subunitPRO_0000190456Add
BLAST

Proteomic databases

PRIDEiP42521.

Expressioni

Developmental stagei

Expression is detected in growing cells and decreases dramatically at the onset of development. The rnrB transcript reappears after the cells have formed multicellular aggregates.1 Publication

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.By similarity

Protein-protein interaction databases

STRINGi44689.DDB_0185062.

Structurei

3D structure databases

ProteinModelPortaliP42521.
SMRiP42521. Positions 12-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
InParanoidiP42521.
KOiK10808.
OMAiCTFACLL.
PhylomeDBiP42521.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42521-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEEINKKDTF IEPILKENKD RFVLFPIKYP DIWRMYKKAL ASHWVAEEID
60 70 80 90 100
LGNDNVDWEY KLTDNERHFI SHVLAFFAAS DGIVNENLAT RFMSEVQIPE
110 120 130 140 150
ARCFYGFQIA IENIHSETYS LLIETYIKDK QTKDKLFNAI ETIPCIKKKA
160 170 180 190 200
EWALRWINDS DSFAERLVAF AAVEGIFFSG SFCSIFWLKK RGLMQGLTFS
210 220 230 240 250
NELISRDEGL HCDFACLLYT KLQRKLDPKV IEKMIRDAVE CEKEFICESL
260 270 280 290 300
PVDLIGMNSR SMSQYIEFCA DRLVVSLGYK KIFNSSNPFE WMEMISLQRK
310 320 330
SNFFEGKVAE YAKTGVAIQG NNQQKNNQSR TLVLDEDF
Length:338
Mass (Da):39,371
Last modified:January 16, 2004 - v2
Checksum:i9BF33B855246CD3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36941 Genomic DNA. Translation: AAB72227.2.
AAFI02000011 Genomic DNA. Translation: EAL70444.1.
AAFI02000009 Genomic DNA. Translation: EAL70945.1.
RefSeqiXP_644369.1. XM_639277.1.
XP_645045.1. XM_639953.1.

Genome annotation databases

EnsemblProtistsiDDB0185062; DDB0185062; DDB_G0272616.
DDB0238222; DDB0238222; DDB_G0274021.
GeneIDi8618721.
8619255.
KEGGiddi:DDB_G0272616.
ddi:DDB_G0274021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36941 Genomic DNA. Translation: AAB72227.2 .
AAFI02000011 Genomic DNA. Translation: EAL70444.1 .
AAFI02000009 Genomic DNA. Translation: EAL70945.1 .
RefSeqi XP_644369.1. XM_639277.1.
XP_645045.1. XM_639953.1.

3D structure databases

ProteinModelPortali P42521.
SMRi P42521. Positions 12-293.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0185062.

Proteomic databases

PRIDEi P42521.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0185062 ; DDB0185062 ; DDB_G0272616 .
DDB0238222 ; DDB0238222 ; DDB_G0274021 .
GeneIDi 8618721.
8619255.
KEGGi ddi:DDB_G0272616.
ddi:DDB_G0274021.

Organism-specific databases

dictyBasei DDB_G0272616. rnrB-1.
DDB_G0274021. rnrB-2.

Phylogenomic databases

eggNOGi COG0208.
InParanoidi P42521.
KOi K10808.
OMAi CTFACLL.
PhylomeDBi P42521.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Miscellaneous databases

PROi P42521.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A prespore-specific gene of Dictyostelium discoideum encodes the small subunit of ribonucleotide reductase."
    Tsang A., Bonfils C., Czaika G., Shtevi A., Grant C.
    Biochim. Biophys. Acta 1309:100-108(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
  2. Tsang A.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 233-237.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiRIR2_DICDI
AccessioniPrimary (citable) accession number: P42521
Secondary accession number(s): Q556I9, Q86AN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 16, 2004
Last modified: November 26, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3