P42521 (RIR2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase small subunit
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase small subunit

Gene names

Name:	rnrB-1
Synonyms:	nrdB
ORF Names:	DDB_G0272616
AND
Name:	rnrB-2
Synonyms:	nrdB
ORF Names:	DDB_G0274021

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large and a small subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Developmental stage	Expression is detected in growing cells and decreases dramatically at the onset of development. The rnrB transcript reappears after the cells have formed multicellular aggregates. Ref.1
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.
Caution	The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from sequence or structural similarity Ref.1. Source: dictyBase deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro response to drug Inferred from sequence or structural similarity. Source: dictyBase
Cellular_component	nucleus Inferred from sequence or structural similarity Ref.1. Source: dictyBase ribonucleoside-diphosphate reductase complex Inferred from sequence or structural similarity Ref.1. Source: dictyBase
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Ribonucleoside-diphosphate reductase small subunit		PRO_0000190456
Sites
	Active site	119	1	By similarity
	Metal binding	81	1	Iron 1 By similarity
	Metal binding	112	1	Iron 1 By similarity
	Metal binding	112	1	Iron 2 By similarity
	Metal binding	115	1	Iron 1 By similarity
	Metal binding	174	1	Iron 2 By similarity
	Metal binding	208	1	Iron 2 By similarity
	Metal binding	211	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42521 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: 9BF33B855246CD3B	FASTA	338	39,371
	10 20 30 40 50 60 MEEINKKDTF IEPILKENKD RFVLFPIKYP DIWRMYKKAL ASHWVAEEID LGNDNVDWEY 70 80 90 100 110 120 KLTDNERHFI SHVLAFFAAS DGIVNENLAT RFMSEVQIPE ARCFYGFQIA IENIHSETYS 130 140 150 160 170 180 LLIETYIKDK QTKDKLFNAI ETIPCIKKKA EWALRWINDS DSFAERLVAF AAVEGIFFSG 190 200 210 220 230 240 SFCSIFWLKK RGLMQGLTFS NELISRDEGL HCDFACLLYT KLQRKLDPKV IEKMIRDAVE 250 260 270 280 290 300 CEKEFICESL PVDLIGMNSR SMSQYIEFCA DRLVVSLGYK KIFNSSNPFE WMEMISLQRK 310 320 330 SNFFEGKVAE YAKTGVAIQG NNQQKNNQSR TLVLDEDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A prespore-specific gene of Dictyostelium discoideum encodes the small subunit of ribonucleotide reductase." Tsang A., Bonfils C., Czaika G., Shtevi A., Grant C. Biochim. Biophys. Acta 1309:100-108(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
[2]	Tsang A. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 233-237.
[3]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L36941 Genomic DNA. Translation: AAB72227.2. AAFI02000011 Genomic DNA. Translation: EAL70444.1. AAFI02000009 Genomic DNA. Translation: EAL70945.1.
RefSeq	XP_644369.1. XM_639277.1. XP_645045.1. XM_639953.1.
3D structure databases
ProteinModelPortal	P42521.
SMR	P42521. Positions 12-293.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0185062.
Proteomic databases
PRIDE	P42521.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185062; DDB0185062; DDB_G0272616. DDB0238222; DDB0238222; DDB_G0274021.
GeneID	8618721. 8619255.
KEGG	ddi:DDB_G0272616. ddi:DDB_G0274021.
Organism-specific databases
dictyBase	DDB_G0272616. rnrB-1. DDB_G0274021. rnrB-2.
Phylogenomic databases
eggNOG	COG0208.
KO	K10808.
OMA	ICNRRCQ.
ProtClustDB	PTZ00211.
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_DICDI

Accession

Primary (citable) accession number: P42521
Secondary accession number(s): Q556I9, Q86AN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 16, 2004
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)