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P42521

- RIR2_DICDI

UniProt

P42521 - RIR2_DICDI

Protein

Ribonucleoside-diphosphate reductase small subunit

Gene

rnrB-1

more
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (16 Jan 2004)
      Previous versions | rss
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    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi81 – 811Iron 1PROSITE-ProRule annotation
    Metal bindingi112 – 1121Iron 1PROSITE-ProRule annotation
    Metal bindingi112 – 1121Iron 2By similarity
    Metal bindingi115 – 1151Iron 1PROSITE-ProRule annotation
    Active sitei119 – 1191PROSITE-ProRule annotation
    Metal bindingi174 – 1741Iron 2By similarity
    Metal bindingi208 – 2081Iron 2By similarity
    Metal bindingi211 – 2111Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. DNA replication Source: dictyBase
    3. response to drug Source: dictyBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_212866. E2F mediated regulation of DNA replication.
    REACT_212891. G1/S-Specific Transcription.
    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase small subunit (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase small subunit
    Gene namesi
    Name:rnrB-1
    Synonyms:nrdB
    ORF Names:DDB_G0272616
    AND
    Name:rnrB-2
    Synonyms:nrdB
    ORF Names:DDB_G0274021
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0272616. rnrB-1.
    DDB_G0274021. rnrB-2.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase
    2. nucleus Source: dictyBase
    3. ribonucleoside-diphosphate reductase complex Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 338338Ribonucleoside-diphosphate reductase small subunitPRO_0000190456Add
    BLAST

    Proteomic databases

    PRIDEiP42521.

    Expressioni

    Developmental stagei

    Expression is detected in growing cells and decreases dramatically at the onset of development. The rnrB transcript reappears after the cells have formed multicellular aggregates.1 Publication

    Interactioni

    Subunit structurei

    Heterodimer of a large and a small subunit.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0185062.

    Structurei

    3D structure databases

    ProteinModelPortaliP42521.
    SMRiP42521. Positions 12-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0208.
    KOiK10808.
    OMAiCTFACLL.
    PhylomeDBiP42521.

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42521-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEINKKDTF IEPILKENKD RFVLFPIKYP DIWRMYKKAL ASHWVAEEID    50
    LGNDNVDWEY KLTDNERHFI SHVLAFFAAS DGIVNENLAT RFMSEVQIPE 100
    ARCFYGFQIA IENIHSETYS LLIETYIKDK QTKDKLFNAI ETIPCIKKKA 150
    EWALRWINDS DSFAERLVAF AAVEGIFFSG SFCSIFWLKK RGLMQGLTFS 200
    NELISRDEGL HCDFACLLYT KLQRKLDPKV IEKMIRDAVE CEKEFICESL 250
    PVDLIGMNSR SMSQYIEFCA DRLVVSLGYK KIFNSSNPFE WMEMISLQRK 300
    SNFFEGKVAE YAKTGVAIQG NNQQKNNQSR TLVLDEDF 338
    Length:338
    Mass (Da):39,371
    Last modified:January 16, 2004 - v2
    Checksum:i9BF33B855246CD3B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36941 Genomic DNA. Translation: AAB72227.2.
    AAFI02000011 Genomic DNA. Translation: EAL70444.1.
    AAFI02000009 Genomic DNA. Translation: EAL70945.1.
    RefSeqiXP_644369.1. XM_639277.1.
    XP_645045.1. XM_639953.1.

    Genome annotation databases

    EnsemblProtistsiDDB0185062; DDB0185062; DDB_G0272616.
    DDB0238222; DDB0238222; DDB_G0274021.
    GeneIDi8618721.
    8619255.
    KEGGiddi:DDB_G0272616.
    ddi:DDB_G0274021.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36941 Genomic DNA. Translation: AAB72227.2 .
    AAFI02000011 Genomic DNA. Translation: EAL70444.1 .
    AAFI02000009 Genomic DNA. Translation: EAL70945.1 .
    RefSeqi XP_644369.1. XM_639277.1.
    XP_645045.1. XM_639953.1.

    3D structure databases

    ProteinModelPortali P42521.
    SMRi P42521. Positions 12-293.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0185062.

    Proteomic databases

    PRIDEi P42521.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0185062 ; DDB0185062 ; DDB_G0272616 .
    DDB0238222 ; DDB0238222 ; DDB_G0274021 .
    GeneIDi 8618721.
    8619255.
    KEGGi ddi:DDB_G0272616.
    ddi:DDB_G0274021.

    Organism-specific databases

    dictyBasei DDB_G0272616. rnrB-1.
    DDB_G0274021. rnrB-2.

    Phylogenomic databases

    eggNOGi COG0208.
    KOi K10808.
    OMAi CTFACLL.
    PhylomeDBi P42521.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .
    Reactomei REACT_212866. E2F mediated regulation of DNA replication.
    REACT_212891. G1/S-Specific Transcription.

    Miscellaneous databases

    PROi P42521.

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A prespore-specific gene of Dictyostelium discoideum encodes the small subunit of ribonucleotide reductase."
      Tsang A., Bonfils C., Czaika G., Shtevi A., Grant C.
      Biochim. Biophys. Acta 1309:100-108(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
    2. Tsang A.
      Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 233-237.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    4. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiRIR2_DICDI
    AccessioniPrimary (citable) accession number: P42521
    Secondary accession number(s): Q556I9, Q86AN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 16, 2004
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Caution

    The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3