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P42521 (RIR2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase small subunit

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase small subunit
Gene names
Name:rnrB-1
Synonyms:nrdB
ORF Names:DDB_G0272616
AND
Name:rnrB-2
Synonyms:nrdB
ORF Names:DDB_G0274021
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Heterodimer of a large and a small subunit By similarity.

Subcellular location

Cytoplasm By similarity.

Developmental stage

Expression is detected in growing cells and decreases dramatically at the onset of development. The rnrB transcript reappears after the cells have formed multicellular aggregates. Ref.1

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase small chain family.

Caution

The gene for this protein is duplicated in strains AX3 and AX4. These strains contain a duplication of a segment of 750 kb of chromosome 2 compared to the corresponding sequence in strain AX2.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 338338Ribonucleoside-diphosphate reductase small subunit
PRO_0000190456

Sites

Active site1191 By similarity
Metal binding811Iron 1 By similarity
Metal binding1121Iron 1 By similarity
Metal binding1121Iron 2 By similarity
Metal binding1151Iron 1 By similarity
Metal binding1741Iron 2 By similarity
Metal binding2081Iron 2 By similarity
Metal binding2111Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P42521 [UniParc].

Last modified January 16, 2004. Version 2.
Checksum: 9BF33B855246CD3B

FASTA33839,371
        10         20         30         40         50         60 
MEEINKKDTF IEPILKENKD RFVLFPIKYP DIWRMYKKAL ASHWVAEEID LGNDNVDWEY 

        70         80         90        100        110        120 
KLTDNERHFI SHVLAFFAAS DGIVNENLAT RFMSEVQIPE ARCFYGFQIA IENIHSETYS 

       130        140        150        160        170        180 
LLIETYIKDK QTKDKLFNAI ETIPCIKKKA EWALRWINDS DSFAERLVAF AAVEGIFFSG 

       190        200        210        220        230        240 
SFCSIFWLKK RGLMQGLTFS NELISRDEGL HCDFACLLYT KLQRKLDPKV IEKMIRDAVE 

       250        260        270        280        290        300 
CEKEFICESL PVDLIGMNSR SMSQYIEFCA DRLVVSLGYK KIFNSSNPFE WMEMISLQRK 

       310        320        330 
SNFFEGKVAE YAKTGVAIQG NNQQKNNQSR TLVLDEDF 

« Hide

References

« Hide 'large scale' references
[1]"A prespore-specific gene of Dictyostelium discoideum encodes the small subunit of ribonucleotide reductase."
Tsang A., Bonfils C., Czaika G., Shtevi A., Grant C.
Biochim. Biophys. Acta 1309:100-108(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
[2]Tsang A.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 233-237.
[3]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[4]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L36941 Genomic DNA. Translation: AAB72227.2.
AAFI02000011 Genomic DNA. Translation: EAL70444.1.
AAFI02000009 Genomic DNA. Translation: EAL70945.1.

3D structure databases

ProteinModelPortalP42521.
SMRP42521. Positions 12-293.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0185062.

Proteomic databases

PRIDEP42521.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0185062; DDB0185062; DDB_G0272616.
DDB0238222; DDB0238222; DDB_G0274021.
KEGGddi:DDB_G0272616.
ddi:DDB_G0274021.

Organism-specific databases

dictyBaseDDB_G0272616. rnrB-1.
DDB_G0274021. rnrB-2.

Phylogenomic databases

eggNOGCOG0208.
KOK10808.
OMAKIFNTEA.
PhylomeDBP42521.
ProtClustDBPTZ00211.

Enzyme and pathway databases

UniPathwayUPA00326.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERPTHR23409. PTHR23409. 1 hit.
PfamPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMSSF47240. SSF47240. 1 hit.
PROSITEPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP42521.

Entry information

Entry nameRIR2_DICDI
AccessionPrimary (citable) accession number: P42521
Secondary accession number(s): Q556I9, Q86AN2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 16, 2004
Last modified: April 16, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase