P42516 (PCL_HALHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-coumarate--CoA ligase
Short name=4CL
EC=6.2.1.12

Alternative name(s):
4-coumaroyl-CoA synthase

Gene names

Name:

pcl

Organism

Halorhodospira halophila (Ectothiorhodospira halophila)

Taxonomic identifier

1053 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Halorhodospira

Protein attributes

Sequence length	391 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore.
Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›391	›391	4-coumarate--CoA ligase		PRO_0000193043
Experimental info
	Non-terminal residue	391	1

Sequences

	Sequence		Length	Mass (Da)
	P42516 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 698321FB6EB2A07D	FASTA	391	42,439
	10 20 30 40 50 60 MQGLNADEVL RLLRSLIPGE LATGRGQRGD PPEAQDLCAD SRLDAEPIRA DSLDRLNLAS 70 80 90 100 110 120 ALNRFFRLHE TGVEDRLLAV RRIGDMAELI ADASQHTSGL TFSTSGSTGT PQPHHHSWAA 130 140 150 160 170 180 LTQEAEALAN ALGSHPRVIA WLPVHHLYGF VFGVALPRAL GSTVIESHAA PTALFREPAP 190 200 210 220 230 240 DDLIATVPAR WRYLFDSNHR FPGGTGISST AALETACRNG LLQAGLDALL EVYGATEAGG 250 260 270 280 290 300 IGLRWAPSED YRLLPHWHGD ATATSSALNP DGAAVTVAPL DRLQWRDERV FRPTGRIDDI 310 320 330 340 350 360 IQIGGVNVSP GHVARRLESH EAVAACAVRS HGEGSRRRLK AFIVPARSDA DPETLRQTLE 370 380 390 NWIWEHLPAV ERPTDLRIGT ELPRNAMGKL Q

References

[1]	"The xanthopsins: a new family of eubacterial blue-light photoreceptors." Kort R., Hoff W.D., van West M., Kroon A.R., Hoffer S.M., Vlieg K.H., Crielaard W., van Beeumen J.J., Hellingwerf K.J. EMBO J. 15:3209-3218(1996) [PubMed: 8670821] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BN9626.
[2]	"Complete chemical structure of photoactive yellow protein: novel thioester-linked 4-hydroxycinnamyl chromophore and photocycle chemistry." Baca M., Borgstahl G.E., Boissinot M., Burke P.M., Williams D.R., Slater K.A., Getzoff E.D. Biochemistry 33:14369-14377(1994) [PubMed: 7981196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112. Strain: BN9626.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98887 Genomic DNA. Translation: CAA67392.1. U17017 Genomic DNA. Translation: AAA61736.1.
PIR	C55993.
3D structure databases
ProteinModelPortal	P42516.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR012743. 4_coum_CoA_lig. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 2 hits. [Graphical view]
TIGRFAMs	TIGR02372. 4_coum_CoA_lig. 1 hit.
PROSITE	PS00455. AMP_BINDING. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCL_HALHA

Accession

Primary (citable) accession number: P42516

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1997
Last modified:	May 31, 2011
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program