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Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Rhodobacter capsulatus (Rhodopseudomonas capsulata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.1 Publication

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei202 – 2021Schiff-base intermediate with substrateBy similarity
Binding sitei212 – 2121Substrate 1By similarity
Binding sitei225 – 2251Substrate 1By similarity
Active sitei256 – 2561Schiff-base intermediate with substrateBy similarity
Binding sitei282 – 2821Substrate 2By similarity
Binding sitei321 – 3211Substrate 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: InterPro
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13250.
UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
OrganismiRhodobacter capsulatus (Rhodopseudomonas capsulata)
Taxonomic identifieri1061 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Delta-aminolevulinic acid dehydratasePRO_0000140508Add
BLAST

Interactioni

Subunit structurei

Homohexamer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP42504.
SMRiP42504. Positions 8-331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42504-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLITPPFPT NRLRRMRRTE ALRDLAQENR LSVKDLIWPI FITDVPGADV
60 70 80 90 100
EISSMPGVVR RTMDGALKAA EGSRDAGHSR DLPVPLTDPA VKTETCEMAW
110 120 130 140 150
QPDNFTNRVI AAMKQAVPEV AIMTDIALDP YNANGHDGLV RDGILLNDET
160 170 180 190 200
TEALVKMALA QAAAGADILG PSDMMDGRVG AIRQAMEAAG HKDIAILSYA
210 220 230 240 250
AKYASAFYGP FRDAVGASSA LKGDKKTYQM NPANSAEALR NVARDIAEGA
260 270 280 290 300
DMVMVKPGMP YLDIVRQVKD AFGMPTYAYQ VSGEYAMLMA AVQNGWLNHD
310 320 330
KVMLESLMAF RRAGCDGVLT YFAPAAAKLI GA
Length:332
Mass (Da):35,854
Last modified:November 1, 1995 - v1
Checksum:i4118DED5A15C8DBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14593 Genomic DNA. Translation: AAA92884.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14593 Genomic DNA. Translation: AAA92884.1.

3D structure databases

ProteinModelPortaliP42504.
SMRiP42504. Positions 8-331.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.
BioCyciMetaCyc:MONOMER-13250.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the Rhodobacter capsulatus hemB gene."
    Indest K., Biel A.J.
    Plant Physiol. 108:421-421(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: PAS100.
  2. "Rhodobacter capsulatus porphobilinogen synthase, a high activity metal ion independent hexamer."
    Bollivar D.W., Clauson C., Lighthall R., Forbes S., Kokona B., Fairman R., Kundrat L., Jaffe E.K.
    BMC Biochem. 5:17-17(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, FUNCTION, SUBUNIT.

Entry informationi

Entry nameiHEM2_RHOCA
AccessioniPrimary (citable) accession number: P42504
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 4, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Does not seem to have a metal requirement for activity.1 Publication

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.