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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

Ba71V-045

Organism
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei177SubstrateBy similarity1
Sitei193Important for hydrogen atom transferBy similarity1
Sitei200Allosteric effector bindingBy similarity1
Binding sitei221Substrate; via amide nitrogenBy similarity1
Sitei230Allosteric effector bindingBy similarity1
Active sitei419Proton acceptorBy similarity1
Active sitei421Cysteine radical intermediateBy similarity1
Active sitei423Proton acceptorBy similarity1
Sitei439Important for hydrogen atom transferBy similarity1
Sitei747Important for electron transferBy similarity1
Sitei748Important for electron transferBy similarity1
Sitei773Interacts with thioredoxin/glutaredoxinBy similarity1
Sitei776Interacts with thioredoxin/glutaredoxinBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Ordered Locus Names:Ba71V-045
ORF Names:F778R
OrganismiAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Taxonomic identifieri10498 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000000624 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872271 – 778Ribonucleoside-diphosphate reductase large subunitAdd BLAST778

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi193 ↔ 439Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP42491.

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP42491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni192 – 193Substrate bindingBy similarity2
Regioni419 – 423Substrate bindingBy similarity5
Regioni613 – 617Substrate bindingBy similarity5

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42491-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METFFIETLA SDVYGKALNV DLDRLSQAQV KYTLQELISY CSALTILHYD
60 70 80 90 100
YSTLAARLSV YQLHQSTASS FSKAVRLQAA QSCSRLSPQF VDVVYKYKAI
110 120 130 140 150
FDSYIDYNRD YKLSLLGIET MKNSYLLKNK DGVIMERPQD AYMRVAIMIY
160 170 180 190 200
GMGKVVNIKM ILLTYDLLSR HVITHASPTM FNAGTKKPQL SSCFLLNVND
210 220 230 240 250
NLENLYDMVK TAGIISGGGG GIGLCLSGIR AKNSFISGSG LRSNGIQNYI
260 270 280 290 300
MLQNASQCYA NQGGLRPGAY AVYLELWHQD IFTFLQMPRL KGQMAEQRLN
310 320 330 340 350
APNLKYGLWV PDLFMEILED QIHNRGDGTW YLFSPDQAPN LHKVFDLERS
360 370 380 390 400
QHENAHREFK KLYYQYVAEK RYTGVTTAKE IIKEWFKTVI QVGNPYIGFK
410 420 430 440 450
DAINRKSNLS HVGTITNSNL CIEVTIPCWE GDKAEQGVCN LAAVNLAAFI
460 470 480 490 500
RENGYDYRGL IEASGNVTEN LDNIIDNGYY PTEATRRSNM RHRPIGIGVF
510 520 530 540 550
GLADVFASLK MKFGSPEAIA MDEAIHAALY YGAMRRSIEL AKEKGSHPSF
560 570 580 590 600
PGSAASKGLL QPDLWVRCGD LSSSWEERVA QTTQGVLTRK SWWQLRLAAM
610 620 630 640 650
QGVRNGYLTA LMPTATSSNS TGKNECFEPF TSNLYTRRTL SGEFIVLNKY
660 670 680 690 700
LIDDLKEIDL WTEAIQQQLL NAGGSIQHIL DIPAEIRDRY KTSREMNQKI
710 720 730 740 750
LTKHAAARNP FVSQSMSLNY YFYEPELSQV LTVLVLGWKK GLTTGSYYCH
760 770
FSPGAGTQKK IIRNSEKACN ADCEACLL
Length:778
Mass (Da):87,492
Last modified:November 1, 1995 - v1
Checksum:i9DB88008677A877F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18466 Genomic DNA. Translation: AAA65275.1.
RefSeqiNP_042739.1. NC_001659.2.

Genome annotation databases

GeneIDi22220427.
KEGGivg:22220427.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18466 Genomic DNA. Translation: AAA65275.1.
RefSeqiNP_042739.1. NC_001659.2.

3D structure databases

ProteinModelPortaliP42491.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP42491.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi22220427.
KEGGivg:22220427.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_ASFB7
AccessioniPrimary (citable) accession number: P42491
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 1, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.