P42463 (ILVB_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetolactate synthase large subunit
Short name=AHAS
EC=2.2.1.6

Alternative name(s):
Acetohydroxy-acid synthase large subunit
Short name=ALS

Gene names

Name:	ilvB
Ordered Locus Names:	Cgl1271, cg1435

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	626 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Subunit structure	Dimer of large and small chains.
Miscellaneous	Contains 1 molecule of FAD per monomer. The role of this cofactor is not clear considering that the reaction does not involve redox chemistry By similarity.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acetolactate synthase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 626	626	Acetolactate synthase large subunit		PRO_0000090787
Regions
	Nucleotide binding	281 – 302	22	FAD By similarity
	Nucleotide binding	324 – 343	20	FAD By similarity
	Region	416 – 496	81	Thiamine pyrophosphate binding
Sites
	Metal binding	467	1	Magnesium By similarity
	Metal binding	494	1	Magnesium By similarity
	Binding site	73	1	Thiamine pyrophosphate By similarity
	Binding site	175	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42463 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C425E93F83B8A946	FASTA	626	66,846
	10 20 30 40 50 60 MNVAASQQPT PATVASRGRS AAPERMTGAK AIVRSLEELN ADIVFGIPGG AVLPVYDPLY 70 80 90 100 110 120 SSTKVRHVLV RHEQGAGHAA TGYAQVTGRV GVCIATSGPG ATNLVTPIAD ANLDSVPMVA 130 140 150 160 170 180 ITGQVGSGLL GTDAFQEADI RGITMPVTKH NFMVTNPNDI PQALAEAFHL AITGRPGPVL 190 200 210 220 230 240 VDIPKDVQNA ELDFVWPPKI DLPGYRPVST PHARQIEQAV KLIGEAKKPV LYVGGGVIKA 250 260 270 280 290 300 DAHEELRAFA EYTGIPVVTT LMALGTFPES HELHMGMPGM HGTVSAVGAL QRSDLLIAIG 310 320 330 340 350 360 SRFDDRVTGD VDTFAPDAKI IHADIDPAEI GKIKQVEVPI VGDAREVLAR LLETTKASKA 370 380 390 400 410 420 ETEDISEWVD YLKGLKARFP RGYDEQPGDL LAPQFVIETL SKEVGPDAIY CAGVGQHQMW 430 440 450 460 470 480 AAQFVDFEKP RTWLNSGGLG TMGYAVPAAL GAKAGAPDKE VWAIDGDGCF QMTNQELTTA 490 500 510 520 530 540 AVEGFPIKIA LINNGNLGMV RQWQTLFYEG RYSNTKLRNQ GEYMPDFVTL SEGLGCVAIR 550 560 570 580 590 600 VTKAEEVLPA IQKAREINDR PVVIDFIVGE DAQVWPMVSA GSSNSDIQYA LGLRPFFDGD 610 620 ESAAEDPADI HEAVSDIDAA VESTEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon." Keilhauer C., Eggeling L., Sahm H. J. Bacteriol. 175:5595-5603(1993) [PubMed: 8366043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L09232 Genomic DNA. Translation: AAA62429.1. BA000036 Genomic DNA. Translation: BAB98664.1. BX927151 Genomic DNA. Translation: CAF19974.1.
PIR	A48648.
RefSeq	NP_600493.1. NC_003450.3. YP_225560.1. NC_006958.1.
3D structure databases
ProteinModelPortal	P42463.
ModBase	Search...
2D gel databases
World-2DPAGE	0001:P42463.
Proteomic databases
PRIDE	P42463.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1019252. 3345149.
GenomeReviews	Gene locus Cgl1271 in contig BA000036_GR. Gene locus cg1435 in contig BX927147_GR.
KEGG	cgb:cg1435. cgl:NCgl1222.
PATRIC	21494584. VBICorGlu203724_1248.
Phylogenomic databases
HOGENOM	HBG323037.
OMA	WQNLYYG.
PhylomeDB	P42463.
ProtClustDB	PRK07789.
Enzyme and pathway databases
BioCyc	CGLU196627:CG1435-MONOMER.
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
KO	K01652.
PANTHER	PTHR18968:SF13. PTHR18968:SF13. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. Acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVB_CORGL

Accession

Primary (citable) accession number: P42463

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program