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Protein

Citrate synthase

Gene

gltA

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Enzyme regulationi

Weakly inhibited by ATP (apparent Ki = 10 mm).

Pathway: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 2-methylcitrate dehydratase 2 (prpD2), Citrate synthase (gltA)
  2. 2-methylcitrate synthase 2 (prpC2), Aconitate hydratase A (acn)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei316 – 3161PROSITE-ProRule annotation
Active sitei372 – 3721PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciCGLU196627:GJDM-824-MONOMER.
UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase (EC:2.3.3.16)
Gene namesi
Name:gltA
Ordered Locus Names:Cgl0829, cg0949
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Citrate synthasePRO_0000169940Add
BLAST

Proteomic databases

PRIDEiP42457.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

STRINGi196627.cg0949.

Structurei

3D structure databases

ProteinModelPortaliP42457.
SMRiP42457. Positions 13-437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000021224.
KOiK01647.
OMAiNKEDGVR.
OrthoDBiEOG6P8TP4.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFERDIVATD NNKAVLHYPG GEFEMDIIEA SEGNNGVVLG KMLSETGLIT
60 70 80 90 100
FDPGYVSTGS TESKITYIDG DAGILRYRGY DIADLAENAT FNEVSYLLIN
110 120 130 140 150
GELPTPDELH KFNDEIRHHT LLDEDFKSQF NVFPRDAHPM ATLASSVNIL
160 170 180 190 200
STYYQDQLNP LDEAQLDKAT VRLMAKVPML AAYAHRARKG APYMYPDNSL
210 220 230 240 250
NARENFLRMM FGYPTEPYEI DPIMVKALDK LLILHADHEQ NCSTSTVRMI
260 270 280 290 300
GSAQANMFVS IAGGINALSG PLHGGANQAV LEMLEDIKSN HGGDATEFMN
310 320 330 340 350
KVKNKEDGVR LMGFGHRVYK NYDPRAAIVK ETAHEILEHL GGDDLLDLAI
360 370 380 390 400
KLEEIALADD YFISRKLYPN VDFYTGLIYR AMGFPTDFFT VLFAIGRLPG
410 420 430
WIAHYREQLG AAGNKINRPR QVYTGNESRK LVPREER
Length:437
Mass (Da):48,929
Last modified:November 1, 1995 - v1
Checksum:i921780BF2C59676B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66112 Genomic DNA. Translation: CAA46902.1.
BA000036 Genomic DNA. Translation: BAB98222.1.
BX927150 Genomic DNA. Translation: CAF19535.1.
PIRiI40717.
RefSeqiNP_600058.1. NC_003450.3.
WP_011013914.1. NC_006958.1.
YP_225121.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98222; BAB98222; BAB98222.
CAF19535; CAF19535; cg0949.
GeneIDi1018824.
KEGGicgb:cg0949.
cgl:NCgl0795.
PATRICi21493706. VBICorGlu203724_0813.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66112 Genomic DNA. Translation: CAA46902.1.
BA000036 Genomic DNA. Translation: BAB98222.1.
BX927150 Genomic DNA. Translation: CAF19535.1.
PIRiI40717.
RefSeqiNP_600058.1. NC_003450.3.
WP_011013914.1. NC_006958.1.
YP_225121.1. NC_006958.1.

3D structure databases

ProteinModelPortaliP42457.
SMRiP42457. Positions 13-437.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg0949.

Proteomic databases

PRIDEiP42457.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB98222; BAB98222; BAB98222.
CAF19535; CAF19535; cg0949.
GeneIDi1018824.
KEGGicgb:cg0949.
cgl:NCgl0795.
PATRICi21493706. VBICorGlu203724_0813.

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000021224.
KOiK01647.
OMAiNKEDGVR.
OrthoDBiEOG6P8TP4.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.
BioCyciCGLU196627:GJDM-824-MONOMER.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence, expression and transcriptional analysis of the Corynebacterium glutamicum gltA gene encoding citrate synthase."
    Eikmanns B.J., Thum-Schmitz N., Eggeling L., Luedtke K.U., Sahm H.
    Microbiology 140:1817-1828(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiCISY_CORGL
AccessioniPrimary (citable) accession number: P42457
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 24, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.