Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P42457 (CISY_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase

EC=2.3.3.16
Gene names
Name:gltA
Ordered Locus Names:Cgl0829, cg0949
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Enzyme regulation

Weakly inhibited by ATP (apparent Ki = 10 mm).

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subunit structure

Homohexamer By similarity.

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Sequence similarities

Belongs to the citrate synthase family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Molecular functionTransferase
   Technical termAllosteric enzyme
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncitrate (Si)-synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 437437Citrate synthase
PRO_0000169940

Sites

Active site3161 By similarity
Active site3721 By similarity

Sequences

Sequence LengthMass (Da)Tools
P42457 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 921780BF2C59676B

FASTA43748,929
        10         20         30         40         50         60 
MFERDIVATD NNKAVLHYPG GEFEMDIIEA SEGNNGVVLG KMLSETGLIT FDPGYVSTGS 

        70         80         90        100        110        120 
TESKITYIDG DAGILRYRGY DIADLAENAT FNEVSYLLIN GELPTPDELH KFNDEIRHHT 

       130        140        150        160        170        180 
LLDEDFKSQF NVFPRDAHPM ATLASSVNIL STYYQDQLNP LDEAQLDKAT VRLMAKVPML 

       190        200        210        220        230        240 
AAYAHRARKG APYMYPDNSL NARENFLRMM FGYPTEPYEI DPIMVKALDK LLILHADHEQ 

       250        260        270        280        290        300 
NCSTSTVRMI GSAQANMFVS IAGGINALSG PLHGGANQAV LEMLEDIKSN HGGDATEFMN 

       310        320        330        340        350        360 
KVKNKEDGVR LMGFGHRVYK NYDPRAAIVK ETAHEILEHL GGDDLLDLAI KLEEIALADD 

       370        380        390        400        410        420 
YFISRKLYPN VDFYTGLIYR AMGFPTDFFT VLFAIGRLPG WIAHYREQLG AAGNKINRPR 

       430 
QVYTGNESRK LVPREER 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence, expression and transcriptional analysis of the Corynebacterium glutamicum gltA gene encoding citrate synthase."
Eikmanns B.J., Thum-Schmitz N., Eggeling L., Luedtke K.U., Sahm H.
Microbiology 140:1817-1828(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X66112 Genomic DNA. Translation: CAA46902.1.
BA000036 Genomic DNA. Translation: BAB98222.1.
BX927150 Genomic DNA. Translation: CAF19535.1.
PIRI40717.
RefSeqNP_600058.1. NC_003450.3.
YP_225121.1. NC_006958.1.

3D structure databases

ProteinModelPortalP42457.
SMRP42457. Positions 13-437.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg0949.

Proteomic databases

PRIDEP42457.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB98222; BAB98222; BAB98222.
CAF19535; CAF19535; cg0949.
GeneID1018824.
KEGGcgb:cg0949.
cgl:NCgl0795.
PATRIC21493706. VBICorGlu203724_0813.

Phylogenomic databases

eggNOGCOG0372.
HOGENOMHOG000021224.
KOK01647.
OMAYSGIIFR.
OrthoDBEOG6P8TP4.

Enzyme and pathway databases

BioCycCGLU196627:GJDM-824-MONOMER.
UniPathwayUPA00223; UER00717.

Family and domain databases

Gene3D1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-II.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFPIRSF001369. Citrate_synth. 1 hit.
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
TIGRFAMsTIGR01798. cit_synth_I. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISY_CORGL
AccessionPrimary (citable) accession number: P42457
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways