ID   SUMT_SYNE7              Reviewed;         243 AA.
AC   P42451; Q31RL6;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   27-MAR-2024, entry version 131.
DE   RecName: Full=Uroporphyrinogen-III C-methyltransferase;
DE            Short=Urogen III methylase;
DE            EC=2.1.1.107 {ECO:0000250|UniProtKB:P21631};
DE   AltName: Full=S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase;
DE            Short=SUMT;
DE   AltName: Full=Uroporphyrinogen III methylase {ECO:0000303|PubMed:8123787};
DE            Short=UROM {ECO:0000303|PubMed:8123787};
GN   Name=cobA {ECO:0000303|PubMed:8123787};
GN   OrderedLocusNames=Synpcc7942_0271;
OS   Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)
OS   (Anacystis nidulans R2).
OC   Bacteria; Cyanobacteriota; Cyanophyceae; Synechococcales; Synechococcaceae;
OC   Synechococcus.
OX   NCBI_TaxID=1140;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8123787; DOI=10.1007/bf00024112;
RA   Jones M.C., Jenkins J.M., Smith A.G., Howe C.J.;
RT   "Cloning and characterisation of genes for tetrapyrrole biosynthesis from
RT   the cyanobacterium Anacystis nidulans R2.";
RL   Plant Mol. Biol. 24:435-448(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33912 / PCC 7942 / FACHB-805;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Lykidis A., Golden S., Richardson P.;
RT   "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the two successive C-2 and C-7 methylation
CC       reactions involved in the conversion of uroporphyrinogen III to
CC       precorrin-2 via the intermediate formation of precorrin-1. It is a step
CC       in the biosynthesis of both cobalamin (vitamin B12) and siroheme.
CC       {ECO:0000250|UniProtKB:P21631}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 S-adenosyl-L-methionine + uroporphyrinogen III = H(+) +
CC         precorrin-2 + 2 S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:32459,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57308, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:58827, ChEBI:CHEBI:59789; EC=2.1.1.107;
CC         Evidence={ECO:0000250|UniProtKB:P21631};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32460;
CC         Evidence={ECO:0000250|UniProtKB:P21631};
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       precorrin-2 from uroporphyrinogen III: step 1/1.
CC       {ECO:0000250|UniProtKB:P21631}.
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme
CC       biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
CC       {ECO:0000250|UniProtKB:P21631}.
CC   -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; X70966; CAA50302.1; -; Genomic_DNA.
DR   EMBL; CP000100; ABB56303.1; -; Genomic_DNA.
DR   RefSeq; WP_011243554.1; NZ_JACJTX010000002.1.
DR   AlphaFoldDB; P42451; -.
DR   SMR; P42451; -.
DR   STRING; 1140.Synpcc7942_0271; -.
DR   PaxDb; 1140-Synpcc7942_0271; -.
DR   GeneID; 76399018; -.
DR   KEGG; syf:Synpcc7942_0271; -.
DR   eggNOG; COG0007; Bacteria.
DR   HOGENOM; CLU_011276_7_0_3; -.
DR   OrthoDB; 9815856at2; -.
DR   BioCyc; SYNEL:SYNPCC7942_0271-MONOMER; -.
DR   UniPathway; UPA00148; UER00211.
DR   UniPathway; UPA00262; UER00211.
DR   Proteomes; UP000889800; Chromosome.
DR   GO; GO:0004851; F:uroporphyrin-III C-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0019354; P:siroheme biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd11642; SUMT; 1.
DR   InterPro; IPR000878; 4pyrrol_Mease.
DR   InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR   InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR   InterPro; IPR014776; 4pyrrole_Mease_sub2.
DR   InterPro; IPR006366; CobA/CysG_C.
DR   InterPro; IPR003043; Uropor_MeTrfase_CS.
DR   NCBIfam; TIGR01469; cobA_cysG_Cterm; 1.
DR   PANTHER; PTHR45790:SF3; S-ADENOSYL-L-METHIONINE-DEPENDENT UROPORPHYRINOGEN III METHYLTRANSFERASE, CHLOROPLASTIC; 1.
DR   PANTHER; PTHR45790; SIROHEME SYNTHASE-RELATED; 1.
DR   Pfam; PF00590; TP_methylase; 1.
DR   SUPFAM; SSF53790; Tetrapyrrole methylase; 1.
DR   PROSITE; PS00839; SUMT_1; 1.
DR   PROSITE; PS00840; SUMT_2; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Methyltransferase; Porphyrin biosynthesis;
KW   Reference proteome; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..243
FT                   /note="Uroporphyrinogen-III C-methyltransferase"
FT                   /id="PRO_0000150374"
FT   BINDING         12
FT                   /ligand="S-adenosyl-L-homocysteine"
FT                   /ligand_id="ChEBI:CHEBI:57856"
FT                   /evidence="ECO:0000250|UniProtKB:P21631"
FT   BINDING         88..90
FT                   /ligand="S-adenosyl-L-homocysteine"
FT                   /ligand_id="ChEBI:CHEBI:57856"
FT                   /evidence="ECO:0000250|UniProtKB:P21631"
FT   BINDING         118..119
FT                   /ligand="S-adenosyl-L-homocysteine"
FT                   /ligand_id="ChEBI:CHEBI:57856"
FT                   /evidence="ECO:0000250|UniProtKB:P21631"
FT   BINDING         166
FT                   /ligand="S-adenosyl-L-homocysteine"
FT                   /ligand_id="ChEBI:CHEBI:57856"
FT                   /evidence="ECO:0000250|UniProtKB:P21631"
FT   BINDING         195
FT                   /ligand="S-adenosyl-L-homocysteine"
FT                   /ligand_id="ChEBI:CHEBI:57856"
FT                   /evidence="ECO:0000250|UniProtKB:P21631"
SQ   SEQUENCE   243 AA;  26463 MW;  9E11AA90724A9870 CRC64;
     MTGKVYLVGA GPGDPEYLTL QAQQCLQSAE VLIYDALIDP RILDLVPANC DRIAVGKRGG
     AESTPQATIN QLLVEHCQQG RKVIRLKSGD PFVFGRAASE LDALERSGCD YAVLPGLSTA
     LAAPLLAGIP ITDPVLSRGF AVYTVHEPDA LNWEALAQLE TLILLMGSRH LLTIGHELIR
     HGRSPDSPVA LIQWAGHPQQ TVLESSLSRM AQQWGDQPLS PCVIVIGEVV RLRKYWAHYR
     YDG
//