P42450 (MASZ_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Synonyms:	aceB
Ordered Locus Names:	Cgl2329, cg2559

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	739 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641
Enzyme regulation	Inhibited by oxalate, glycolate and ATP. HAMAP-Rule MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641
Subunit structure	Monomer.
Subcellular location	Cytoplasm HAMAP-Rule MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 739	738	Malate synthase G HAMAP-Rule MF_00641		PRO_0000166885
Sites
	Active site	356	1	Proton acceptor By similarity
	Active site	647	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42450 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: DE9AC15F65CCC8EB	FASTA	739	82,363
	10 20 30 40 50 60 MTEQELLSAQ TADNAGTDST ERVDAGGMQV AKVLYDFVTE AVLPRVGVDA EKFWSGFAAI 70 80 90 100 110 120 ARDLTPRNRE LLARRDELQM LIDDYHRNNS GTIDQEAYED FLKEIGYLVE EPEAAEIRTQ 130 140 150 160 170 180 NVDTEISSTA GPQLVVPILN ARFALNAANA RWGSLYDALY GTNAIPETDG AEKGKEYNPV 190 200 210 220 230 240 RGQKVIEWGR EFLDSVVPLD GASHADVEKY NITDGKLAAH IGDSVYRLKN RESYRGFTGN 250 260 270 280 290 300 FLDPEAILLE TNGLHIELQI DPVHPIGKAD KTGLKDIVLE SAITTIMDFE DSVAAVDAED 310 320 330 340 350 360 KTLGYSNWFG LNTGELKEEM SKNGRIFTRE LNKDRVYIGR NGTELVLHGR SLLFVRNVGH 370 380 390 400 410 420 LMQNPSILID GEEIFEGIMD AVLTTVCAIP GIAPQNKMRN SRKGSIYIVK PKQHGPEEVA 430 440 450 460 470 480 FTNELFGRVE DLLDLPRHTL KVGVMDEERR TSVNLDASIM EVADRLAFIN TGFLDRTGDE 490 500 510 520 530 540 IHTSMEAGAM VRKADMQTAP WKQAYENNNV DAGIQRGLPG KAQIGKGMWA MTELMAEMLE 550 560 570 580 590 600 KKIGQPREGA NTAWVPSPTG ATLHATHYHL VDVFKVQDEL RAAGRRDSLR NILTIPTAPN 610 620 630 640 650 660 TNWSEEEKKE EMDNNCQSIL GYVVRWVEHG VGCSKVPDIH DIDLMEDRAT LRISSQMLAN 670 680 690 700 710 720 WIRHDVVSKE QVLESLERMA VVVDKQNAGD EAYRDMAPNY DASLAFQAAK DLIFEGTKSP 730 SGYTEPILHA RRREFKAKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Malate synthase from Corynebacterium glutamicum: sequence analysis of the gene and biochemical characterization of the enzyme." Reinscheid D.J., Eikmanns B.J., Sahm H. Microbiology 140:3099-3108(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"Molecular characterization of aceB, a gene encoding malate synthase in Corynebacterium glutamicum." Lee H.S., Sinskey A.J. J. Microbiol. Biotechnol. 4:256-263(1994) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[3]	"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78491 Genomic DNA. Translation: CAA55243.1. L27123 Genomic DNA. Translation: AAA68074.1. BA000036 Genomic DNA. Translation: BAB99722.1. BX927154 Genomic DNA. Translation: CAF20673.1.
PIR	I40715.
RefSeq	NP_601530.1. NC_003450.3. YP_226574.1. NC_006958.1.
3D structure databases
ProteinModelPortal	P42450.
SMR	P42450. Positions 20-738.
ModBase	Search...
Protein-protein interaction databases
STRING	196627.cg2559.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB99722; BAB99722; BAB99722. CAF20673; CAF20673; cg2559.
GeneID	1020280. 3345106.
KEGG	cgb:cg2559. cgl:NCgl2247.
PATRIC	21496662. VBICorGlu203724_2263.
Phylogenomic databases
eggNOG	COG2225.
KO	K01638.
OMA	VVKPKMH.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	CGLU196627:GJDM-2306-MONOMER.
BRENDA	2.3.3.9. 1648.
UniPathway	UPA00703; UER00720.
Family and domain databases
Gene3D	2.170.170.11. 2 hits.
HAMAP	MF_00641. Malate_synth_G.
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_CORGL

Accession

Primary (citable) accession number: P42450

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program