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P42450

- MASZ_CORGL

UniProt

P42450 - MASZ_CORGL

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Protein
Malate synthase G
Gene
glcB, aceB, Cgl2329, cg2559
Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.1 Publication

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.1 Publication

Cofactori

Magnesium. Cobalt or Mnaganese could partially replace magnesium.1 Publication

Enzyme regulationi

Inhibited by oxalate, glycolate and ATP.1 Publication

Kineticsi

  1. KM=12 µM for acetyl-CoA (at pH 7.6 and 30 degrees Celsius)1 Publication
  2. KM=30 µM for glyoxylate (at pH 7.6 and 30 degrees Celsius)

pH dependencei

Optimum pH is 7.6.

Temperature dependencei

Optimum temperature is 43 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei135 – 1351Acetyl-CoA; via carbonyl oxygen By similarity
Binding sitei292 – 2921Acetyl-CoA By similarity
Binding sitei329 – 3291Acetyl-CoA By similarity
Active sitei356 – 3561Proton acceptor By similarity
Binding sitei356 – 3561Glyoxylate By similarity
Metal bindingi447 – 4471Magnesium By similarity
Binding sitei447 – 4471Glyoxylate By similarity
Metal bindingi475 – 4751Magnesium By similarity
Binding sitei556 – 5561Acetyl-CoA; via carbonyl oxygen By similarity
Active sitei647 – 6471Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi2.3.3.9. 1648.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G (EC:2.3.3.9)
Gene namesi
Name:glcB
Synonyms:aceB
Ordered Locus Names:Cgl2329, cg2559
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show the absence of malate synthase activity and to the inability to grow on acetate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 739738Malate synthase GUniRule annotation
PRO_0000166885Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei633 – 6331Cysteine sulfenic acid (-SOH) By similarity

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi196627.cg2559.

Structurei

3D structure databases

ProteinModelPortaliP42450.
SMRiP42450. Positions 20-738.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni142 – 1432Acetyl-CoA binding By similarity
Regioni472 – 4754Glyoxylate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2225.
KOiK01638.
OMAiSTIMDFE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42450-1 [UniParc]FASTAAdd to Basket

« Hide

MTEQELLSAQ TADNAGTDST ERVDAGGMQV AKVLYDFVTE AVLPRVGVDA    50
EKFWSGFAAI ARDLTPRNRE LLARRDELQM LIDDYHRNNS GTIDQEAYED 100
FLKEIGYLVE EPEAAEIRTQ NVDTEISSTA GPQLVVPILN ARFALNAANA 150
RWGSLYDALY GTNAIPETDG AEKGKEYNPV RGQKVIEWGR EFLDSVVPLD 200
GASHADVEKY NITDGKLAAH IGDSVYRLKN RESYRGFTGN FLDPEAILLE 250
TNGLHIELQI DPVHPIGKAD KTGLKDIVLE SAITTIMDFE DSVAAVDAED 300
KTLGYSNWFG LNTGELKEEM SKNGRIFTRE LNKDRVYIGR NGTELVLHGR 350
SLLFVRNVGH LMQNPSILID GEEIFEGIMD AVLTTVCAIP GIAPQNKMRN 400
SRKGSIYIVK PKQHGPEEVA FTNELFGRVE DLLDLPRHTL KVGVMDEERR 450
TSVNLDASIM EVADRLAFIN TGFLDRTGDE IHTSMEAGAM VRKADMQTAP 500
WKQAYENNNV DAGIQRGLPG KAQIGKGMWA MTELMAEMLE KKIGQPREGA 550
NTAWVPSPTG ATLHATHYHL VDVFKVQDEL RAAGRRDSLR NILTIPTAPN 600
TNWSEEEKKE EMDNNCQSIL GYVVRWVEHG VGCSKVPDIH DIDLMEDRAT 650
LRISSQMLAN WIRHDVVSKE QVLESLERMA VVVDKQNAGD EAYRDMAPNY 700
DASLAFQAAK DLIFEGTKSP SGYTEPILHA RRREFKAKN 739
Length:739
Mass (Da):82,363
Last modified:January 23, 2007 - v2
Checksum:iDE9AC15F65CCC8EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78491 Genomic DNA. Translation: CAA55243.1.
L27123 Genomic DNA. Translation: AAA68074.1.
BA000036 Genomic DNA. Translation: BAB99722.1.
BX927154 Genomic DNA. Translation: CAF20673.1.
PIRiI40715.
RefSeqiNP_601530.1. NC_003450.3.
WP_011015044.1. NC_006958.1.
YP_226574.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99722; BAB99722; BAB99722.
CAF20673; CAF20673; cg2559.
GeneIDi1020280.
KEGGicgb:cg2559.
cgl:NCgl2247.
PATRICi21496662. VBICorGlu203724_2263.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78491 Genomic DNA. Translation: CAA55243.1 .
L27123 Genomic DNA. Translation: AAA68074.1 .
BA000036 Genomic DNA. Translation: BAB99722.1 .
BX927154 Genomic DNA. Translation: CAF20673.1 .
PIRi I40715.
RefSeqi NP_601530.1. NC_003450.3.
WP_011015044.1. NC_006958.1.
YP_226574.1. NC_006958.1.

3D structure databases

ProteinModelPortali P42450.
SMRi P42450. Positions 20-738.
ModBasei Search...

Protein-protein interaction databases

STRINGi 196627.cg2559.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB99722 ; BAB99722 ; BAB99722 .
CAF20673 ; CAF20673 ; cg2559 .
GeneIDi 1020280.
KEGGi cgb:cg2559.
cgl:NCgl2247.
PATRICi 21496662. VBICorGlu203724_2263.

Phylogenomic databases

eggNOGi COG2225.
KOi K01638.
OMAi STIMDFE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BRENDAi 2.3.3.9. 1648.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Malate synthase from Corynebacterium glutamicum: sequence analysis of the gene and biochemical characterization of the enzyme."
    Reinscheid D.J., Eikmanns B.J., Sahm H.
    Microbiology 140:3099-3108(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, COFACTOR, SUBUNIT.
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. "Molecular characterization of aceB, a gene encoding malate synthase in Corynebacterium glutamicum."
    Lee H.S., Sinskey A.J.
    J. Microbiol. Biotechnol. 4:256-263(1994)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
  3. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiMASZ_CORGL
AccessioniPrimary (citable) accession number: P42450
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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