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Reviewed, UniProtKB/Swiss-Prot P42450 (MASZ_CORGL)

Last modified November 3, 2009. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Malate synthase G
    EC=2.3.3.9
Gene names
Name: glcB
Synonyms: aceB
Ordered Locus Names: Cgl2329, cg2559
OrganismCorynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]
Taxonomic identifier1718 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length739 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641

Enzyme regulation

Inhibited by oxalate, glycolate and ATP. HAMAP MF_00641

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641

Subunit structure

Monomer. HAMAP MF_00641

Subcellular location

Cytoplasm. HAMAP MF_00641

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processglyoxylate cycle

Inferred from electronic annotation. Source: HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionmalate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 739738Malate synthase G HAMAP MF_00641
PRO_0000166885

Sites

Active site3561Proton acceptor By similarity
Active site6471Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P42450-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: DE9AC15F65CCC8EB

FASTA73982,363
        10         20         30         40         50         60 
MTEQELLSAQ TADNAGTDST ERVDAGGMQV AKVLYDFVTE AVLPRVGVDA EKFWSGFAAI 

        70         80         90        100        110        120 
ARDLTPRNRE LLARRDELQM LIDDYHRNNS GTIDQEAYED FLKEIGYLVE EPEAAEIRTQ 

       130        140        150        160        170        180 
NVDTEISSTA GPQLVVPILN ARFALNAANA RWGSLYDALY GTNAIPETDG AEKGKEYNPV 

       190        200        210        220        230        240 
RGQKVIEWGR EFLDSVVPLD GASHADVEKY NITDGKLAAH IGDSVYRLKN RESYRGFTGN 

       250        260        270        280        290        300 
FLDPEAILLE TNGLHIELQI DPVHPIGKAD KTGLKDIVLE SAITTIMDFE DSVAAVDAED 

       310        320        330        340        350        360 
KTLGYSNWFG LNTGELKEEM SKNGRIFTRE LNKDRVYIGR NGTELVLHGR SLLFVRNVGH 

       370        380        390        400        410        420 
LMQNPSILID GEEIFEGIMD AVLTTVCAIP GIAPQNKMRN SRKGSIYIVK PKQHGPEEVA 

       430        440        450        460        470        480 
FTNELFGRVE DLLDLPRHTL KVGVMDEERR TSVNLDASIM EVADRLAFIN TGFLDRTGDE 

       490        500        510        520        530        540 
IHTSMEAGAM VRKADMQTAP WKQAYENNNV DAGIQRGLPG KAQIGKGMWA MTELMAEMLE 

       550        560        570        580        590        600 
KKIGQPREGA NTAWVPSPTG ATLHATHYHL VDVFKVQDEL RAAGRRDSLR NILTIPTAPN 

       610        620        630        640        650        660 
TNWSEEEKKE EMDNNCQSIL GYVVRWVEHG VGCSKVPDIH DIDLMEDRAT LRISSQMLAN 

       670        680        690        700        710        720 
WIRHDVVSKE QVLESLERMA VVVDKQNAGD EAYRDMAPNY DASLAFQAAK DLIFEGTKSP 

       730 
SGYTEPILHA RRREFKAKN 

« Hide

References

« Hide 'large scale' references
[1]"Malate synthase from Corynebacterium glutamicum: sequence analysis of the gene and biochemical characterization of the enzyme."
Reinscheid D.J., Eikmanns B.J., Sahm H.
Microbiology 140:3099-3108(1994) [PubMed: 7812449] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION.
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]"Molecular characterization of aceB, a gene encoding malate synthase in Corynebacterium glutamicum."
Lee H.S., Sinskey A.J.
J. Microbiol. Biotechnol. 4:256-263(1994)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[3]"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032."
Nakagawa S.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

X78491 Genomic DNA. Translation: CAA55243.1.
L27123 Genomic DNA. Translation: AAA68074.1.
BA000036 Genomic DNA. Translation: BAB99722.1.
BX927154 Genomic DNA. Translation: CAF20673.1.
PIRI40715.
RefSeqNP_601530.1.
YP_226574.1.

3D structure databases

HSSPHSSP built from PDB template 1N8I based on UniProtKB Q50596.
ModBaseSearch...

Genome annotation databases

GeneID1020280.
3345106.
GenomeReviewsGene locus Cgl2329 in contig BA000036_GR.
Gene locus cg2559 in contig BX927147_GR.
KEGGcgb:cg2559.
cgl:NCgl2247.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP42450.
OMAPKMHGPD.

Enzyme and pathway databases

BioCycCGLU196627-1:CG2559-MON.
BRENDA2.3.3.9. 812.

Family and domain databases

HAMAPMF_00641.
[Tree]
InterProIPR011076. Malate_synth-like_core.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PANTHERPTHR21631:SF1. Malate_synthase. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01345. malate_syn_G. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMASZ_CORGL
AccessionPrimary (citable) accession number: P42450
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 3, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents