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P42449

- ACEA_CORGL

UniProt

P42449 - ACEA_CORGL

Protein

Isocitrate lyase

Gene

aceA

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase-positive serine pathway By similarity.By similarity

    Catalytic activityi

    Isocitrate = succinate + glyoxylate.PROSITE-ProRule annotation

    Cofactori

    Divalent cations. Magnesium, manganese and cobalt can be used.

    Enzyme regulationi

    Inhibited by 3-phosphoglycerate, 6-phosphogluconate, PEP, fructose 1,6-bisphosphate, and succinate.

    pH dependencei

    Optimum pH is 7.3.

    Temperature dependencei

    Optimum temperature is 40 degrees Celsius.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei193 – 1931Curated

    GO - Molecular functioni

    1. isocitrate lyase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-UniPathway
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00719.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate lyase (EC:4.1.3.1)
    Short name:
    ICL
    Short name:
    Isocitrase
    Short name:
    Isocitratase
    Gene namesi
    Name:aceA
    Ordered Locus Names:Cgl2331, cg2560
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 432431Isocitrate lyasePRO_0000068773Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    DIPiDIP-2898N.
    STRINGi196627.cg2560.

    Structurei

    3D structure databases

    ProteinModelPortaliP42449.
    SMRiP42449. Positions 2-430.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2224.
    HOGENOMiHOG000238475.
    KOiK01637.
    OMAiKEWWASP.
    OrthoDBiEOG689HMX.

    Family and domain databases

    Gene3Di3.20.20.60. 1 hit.
    InterProiIPR006254. Isocitrate_lyase.
    IPR018523. Isocitrate_lyase_ph_CS.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
    PfamiPF00463. ICL. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001362. Isocit_lyase. 1 hit.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    TIGRFAMsiTIGR01346. isocit_lyase. 2 hits.
    PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P42449-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNVGKPRTA QEIQQDWDTN PRWNGITRDY TADQVADLQG SVIEEHTLAR    50
    RGSEILWDAV TQEGDGYINA LGALTGNQAV QQVRAGLKAV YLSGWQVAGD 100
    ANLSGHTYPD QSLYPANSVP SVVRRINNAL LRSDEIARTE GDTSVDNWVV 150
    PIVADGEAGF GGALNVYELQ KAMIAAGAAG THWEDQLASE KKCGHLGGKV 200
    LIPTQQHIRT LNSARLAADV ANTPTVVIAR TDAEAATLIT SDVDERDQPF 250
    ITGERTAEGY YHVKNGLEPC IARAKSYAPY ADMIWMETGT PDLELAKKFA 300
    EGVRSEFPDQ LLSYNCSPSF NWSAHLEADE IAKFQKELGA MGFKFQFITL 350
    AGFHSLNYGM FDLAYGYARE GMTSFVDLQN REFKAAEERG FTAVKHQREV 400
    GAGYFDQIAT TVDPNSSTTA LKGSTEEGQF HN 432
    Length:432
    Mass (Da):47,228
    Last modified:January 23, 2007 - v2
    Checksum:iF8B50DA176D32E87
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75504 Genomic DNA. Translation: CAA53219.1.
    BA000036 Genomic DNA. Translation: BAB99724.1.
    BX927154 Genomic DNA. Translation: CAF20674.1.
    PIRiI40713.
    RefSeqiNP_601531.1. NC_003450.3.
    YP_226575.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB99724; BAB99724; BAB99724.
    CAF20674; CAF20674; cg2560.
    GeneIDi1020281.
    KEGGicgb:cg2560.
    cgl:NCgl2248.
    PATRICi21496664. VBICorGlu203724_2264.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75504 Genomic DNA. Translation: CAA53219.1 .
    BA000036 Genomic DNA. Translation: BAB99724.1 .
    BX927154 Genomic DNA. Translation: CAF20674.1 .
    PIRi I40713.
    RefSeqi NP_601531.1. NC_003450.3.
    YP_226575.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali P42449.
    SMRi P42449. Positions 2-430.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-2898N.
    STRINGi 196627.cg2560.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB99724 ; BAB99724 ; BAB99724 .
    CAF20674 ; CAF20674 ; cg2560 .
    GeneIDi 1020281.
    KEGGi cgb:cg2560.
    cgl:NCgl2248.
    PATRICi 21496664. VBICorGlu203724_2264.

    Phylogenomic databases

    eggNOGi COG2224.
    HOGENOMi HOG000238475.
    KOi K01637.
    OMAi KEWWASP.
    OrthoDBi EOG689HMX.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00719 .

    Family and domain databases

    Gene3Di 3.20.20.60. 1 hit.
    InterProi IPR006254. Isocitrate_lyase.
    IPR018523. Isocitrate_lyase_ph_CS.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    PANTHERi PTHR21631:SF3. PTHR21631:SF3. 1 hit.
    Pfami PF00463. ICL. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001362. Isocit_lyase. 1 hit.
    SUPFAMi SSF51621. SSF51621. 1 hit.
    TIGRFAMsi TIGR01346. isocit_lyase. 2 hits.
    PROSITEi PS00161. ISOCITRATE_LYASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme."
      Reinscheid D.J., Eikmanns B.J., Sahm H.
      J. Bacteriol. 176:3474-3483(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION.
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiACEA_CORGL
    AccessioniPrimary (citable) accession number: P42449
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3