P42449 (ACEA_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate lyase
Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1

Gene names

Name:	aceA
Ordered Locus Names:	Cgl2331, cg2560

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase-positive serine pathway By similarity.
Catalytic activity	Isocitrate = succinate + glyoxylate.
Cofactor	Divalent cations. Magnesium, manganese and cobalt can be used.
Enzyme regulation	Inhibited by 3-phosphoglycerate, 6-phosphogluconate, PEP, fructose 1,6-bisphosphate, and succinate.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.3. Temperature dependence: Optimum temperature is 40 degrees Celsius.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isocitrate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 432	431	Isocitrate lyase		PRO_0000068773
Sites
	Active site	193	1	Probable

Sequences

	Sequence		Length	Mass (Da)
	P42449 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: F8B50DA176D32E87	FASTA	432	47,228
	10 20 30 40 50 60 MSNVGKPRTA QEIQQDWDTN PRWNGITRDY TADQVADLQG SVIEEHTLAR RGSEILWDAV 70 80 90 100 110 120 TQEGDGYINA LGALTGNQAV QQVRAGLKAV YLSGWQVAGD ANLSGHTYPD QSLYPANSVP 130 140 150 160 170 180 SVVRRINNAL LRSDEIARTE GDTSVDNWVV PIVADGEAGF GGALNVYELQ KAMIAAGAAG 190 200 210 220 230 240 THWEDQLASE KKCGHLGGKV LIPTQQHIRT LNSARLAADV ANTPTVVIAR TDAEAATLIT 250 260 270 280 290 300 SDVDERDQPF ITGERTAEGY YHVKNGLEPC IARAKSYAPY ADMIWMETGT PDLELAKKFA 310 320 330 340 350 360 EGVRSEFPDQ LLSYNCSPSF NWSAHLEADE IAKFQKELGA MGFKFQFITL AGFHSLNYGM 370 380 390 400 410 420 FDLAYGYARE GMTSFVDLQN REFKAAEERG FTAVKHQREV GAGYFDQIAT TVDPNSSTTA 430 LKGSTEEGQF HN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme." Reinscheid D.J., Eikmanns B.J., Sahm H. J. Bacteriol. 176:3474-3483(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75504 Genomic DNA. Translation: CAA53219.1. BA000036 Genomic DNA. Translation: BAB99724.1. BX927154 Genomic DNA. Translation: CAF20674.1.
PIR	I40713.
RefSeq	NP_601531.1. NC_003450.3. YP_226575.1. NC_006958.1.
3D structure databases
ProteinModelPortal	P42449.
SMR	P42449. Positions 2-430.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2898N.
STRING	196627.cg2560.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB99724; BAB99724; BAB99724. CAF20674; CAF20674; cg2560.
GeneID	1020281. 3345421.
KEGG	cgb:cg2560. cgl:NCgl2248.
PATRIC	21496664. VBICorGlu203724_2264.
Phylogenomic databases
eggNOG	COG2224.
HOGENOM	HOG000238475.
KO	K01637.
OMA	QAVQQVK.
ProtClustDB	PRK15063.
Enzyme and pathway databases
BioCyc	CGLU196627:GJDM-2307-MONOMER.
UniPathway	UPA00703; UER00719.
Family and domain databases
Gene3D	3.20.20.60. 1 hit.
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
PANTHER	PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfam	PF00463. ICL. 1 hit. [Graphical view]
PIRSF	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01346. isocit_lyase. 2 hits.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACEA_CORGL

Accession

Primary (citable) accession number: P42449

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program