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Protein

Isocitrate lyase

Gene

aceA

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the metabolic adaptation in response to environmental changes. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates.1 Publication

Catalytic activityi

Isocitrate = succinate + glyoxylate.1 Publication

Cofactori

Mg2+1 PublicationNote: Divalent metal cations. Mn(2+) or Co2+ can be used.1 Publication

Enzyme regulationi

Inhibited by 3-phosphoglycerate, 6-phosphogluconate, phosphoenolpyruvate (PEP), fructose 1,6-bisphosphate, glycolate, oxalate, and itaconate.1 Publication

Kineticsi

  1. KM=0.28 mM for threo-D-isocitrate.1 Publication
  2. KM=0.34 mM for glyoxylate.1 Publication
  3. KM=0.61 mM for succinate.1 Publication

pH dependencei

Optimum pH is 7.3.1 Publication

Temperature dependencei

Optimum temperature is 40 degrees Celsius. Above 45 degrees Celsius, the enzyme is denaturated.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi155 – 1551MagnesiumBy similarity
Active sitei193 – 1931Proton acceptorBy similarity
Binding sitei230 – 2301SubstrateBy similarity
Binding sitei349 – 3491SubstrateBy similarity

GO - Molecular functioni

  1. isocitrate lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-UniPathway
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Cobalt, Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyase1 Publication (EC:4.1.3.11 Publication)
Short name:
ICL1 Publication
Alternative name(s):
Isocitrase1 Publication
Isocitratase1 Publication
Gene namesi
Name:aceA1 Publication
Ordered Locus Names:Cgl2331, cg2560
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001009 Componenti: Chromosome UP000000582 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are unable to grow on acetate as carbon source.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 432431Isocitrate lyasePRO_0000068773Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

DIPiDIP-2898N.
STRINGi196627.cg2560.

Structurei

3D structure databases

ProteinModelPortaliP42449.
SMRiP42449. Positions 2-430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 953Substrate bindingBy similarity
Regioni194 – 1952Substrate bindingBy similarity
Regioni315 – 3195Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2224.
HOGENOMiHOG000238475.
KOiK01637.
OMAiRWKGVTR.
OrthoDBiEOG689HMX.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 2 hits.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42449-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNVGKPRTA QEIQQDWDTN PRWNGITRDY TADQVADLQG SVIEEHTLAR
60 70 80 90 100
RGSEILWDAV TQEGDGYINA LGALTGNQAV QQVRAGLKAV YLSGWQVAGD
110 120 130 140 150
ANLSGHTYPD QSLYPANSVP SVVRRINNAL LRSDEIARTE GDTSVDNWVV
160 170 180 190 200
PIVADGEAGF GGALNVYELQ KAMIAAGAAG THWEDQLASE KKCGHLGGKV
210 220 230 240 250
LIPTQQHIRT LNSARLAADV ANTPTVVIAR TDAEAATLIT SDVDERDQPF
260 270 280 290 300
ITGERTAEGY YHVKNGLEPC IARAKSYAPY ADMIWMETGT PDLELAKKFA
310 320 330 340 350
EGVRSEFPDQ LLSYNCSPSF NWSAHLEADE IAKFQKELGA MGFKFQFITL
360 370 380 390 400
AGFHSLNYGM FDLAYGYARE GMTSFVDLQN REFKAAEERG FTAVKHQREV
410 420 430
GAGYFDQIAT TVDPNSSTTA LKGSTEEGQF HN
Length:432
Mass (Da):47,228
Last modified:January 23, 2007 - v2
Checksum:iF8B50DA176D32E87
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75504 Genomic DNA. Translation: CAA53219.1.
BA000036 Genomic DNA. Translation: BAB99724.1.
BX927154 Genomic DNA. Translation: CAF20674.1.
PIRiI40713.
RefSeqiNP_601531.1. NC_003450.3.
YP_226575.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99724; BAB99724; BAB99724.
GeneIDi1020281.
KEGGicgb:cg2560.
cgl:NCgl2248.
PATRICi21496664. VBICorGlu203724_2264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75504 Genomic DNA. Translation: CAA53219.1.
BA000036 Genomic DNA. Translation: BAB99724.1.
BX927154 Genomic DNA. Translation: CAF20674.1.
PIRiI40713.
RefSeqiNP_601531.1. NC_003450.3.
YP_226575.1. NC_006958.1.

3D structure databases

ProteinModelPortaliP42449.
SMRiP42449. Positions 2-430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-2898N.
STRINGi196627.cg2560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB99724; BAB99724; BAB99724.
GeneIDi1020281.
KEGGicgb:cg2560.
cgl:NCgl2248.
PATRICi21496664. VBICorGlu203724_2264.

Phylogenomic databases

eggNOGiCOG2224.
HOGENOMiHOG000238475.
KOiK01637.
OMAiRWKGVTR.
OrthoDBiEOG689HMX.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 2 hits.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme."
    Reinscheid D.J., Eikmanns B.J., Sahm H.
    J. Bacteriol. 176:3474-3483(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, ENZYME REGULATION, COFACTOR, SUBUNIT.
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiACEA_CORGL
AccessioniPrimary (citable) accession number: P42449
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.