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P42449 (ACEA_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate lyase

Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1
Gene names
Name:aceA
Ordered Locus Names:Cgl2331, cg2560
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase-positive serine pathway By similarity.

Catalytic activity

Isocitrate = succinate + glyoxylate.

Cofactor

Divalent cations. Magnesium, manganese and cobalt can be used.

Enzyme regulation

Inhibited by 3-phosphoglycerate, 6-phosphogluconate, PEP, fructose 1,6-bisphosphate, and succinate.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.3.

Temperature dependence:

Optimum temperature is 40 degrees Celsius.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   Molecular functionLyase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionisocitrate lyase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 432431Isocitrate lyase
PRO_0000068773

Sites

Active site1931 Probable

Sequences

Sequence LengthMass (Da)Tools
P42449 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: F8B50DA176D32E87

FASTA43247,228
        10         20         30         40         50         60 
MSNVGKPRTA QEIQQDWDTN PRWNGITRDY TADQVADLQG SVIEEHTLAR RGSEILWDAV 

        70         80         90        100        110        120 
TQEGDGYINA LGALTGNQAV QQVRAGLKAV YLSGWQVAGD ANLSGHTYPD QSLYPANSVP 

       130        140        150        160        170        180 
SVVRRINNAL LRSDEIARTE GDTSVDNWVV PIVADGEAGF GGALNVYELQ KAMIAAGAAG 

       190        200        210        220        230        240 
THWEDQLASE KKCGHLGGKV LIPTQQHIRT LNSARLAADV ANTPTVVIAR TDAEAATLIT 

       250        260        270        280        290        300 
SDVDERDQPF ITGERTAEGY YHVKNGLEPC IARAKSYAPY ADMIWMETGT PDLELAKKFA 

       310        320        330        340        350        360 
EGVRSEFPDQ LLSYNCSPSF NWSAHLEADE IAKFQKELGA MGFKFQFITL AGFHSLNYGM 

       370        380        390        400        410        420 
FDLAYGYARE GMTSFVDLQN REFKAAEERG FTAVKHQREV GAGYFDQIAT TVDPNSSTTA 

       430 
LKGSTEEGQF HN 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme."
Reinscheid D.J., Eikmanns B.J., Sahm H.
J. Bacteriol. 176:3474-3483(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION.
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X75504 Genomic DNA. Translation: CAA53219.1.
BA000036 Genomic DNA. Translation: BAB99724.1.
BX927154 Genomic DNA. Translation: CAF20674.1.
PIRI40713.
RefSeqNP_601531.1. NC_003450.3.
YP_226575.1. NC_006958.1.

3D structure databases

ProteinModelPortalP42449.
SMRP42449. Positions 2-430.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-2898N.
STRING196627.cg2560.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB99724; BAB99724; BAB99724.
CAF20674; CAF20674; cg2560.
GeneID1020281.
3345421.
KEGGcgb:cg2560.
cgl:NCgl2248.
PATRIC21496664. VBICorGlu203724_2264.

Phylogenomic databases

eggNOGCOG2224.
HOGENOMHOG000238475.
KOK01637.
OMALEKDWAE.
OrthoDBEOG689HMX.
ProtClustDBPRK15063.

Enzyme and pathway databases

UniPathwayUPA00703; UER00719.

Family and domain databases

Gene3D3.20.20.60. 1 hit.
InterProIPR006254. Isocitrate_lyase.
IPR000918. Isocitrate_lyase/Pmutase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamPF00463. ICL. 1 hit.
[Graphical view]
PIRSFPIRSF001362. Isocit_lyase. 1 hit.
SUPFAMSSF51621. SSF51621. 1 hit.
TIGRFAMsTIGR01346. isocit_lyase. 2 hits.
PROSITEPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACEA_CORGL
AccessionPrimary (citable) accession number: P42449
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 13, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways