Reviewed, UniProtKB/Swiss-Prot P42445 (RECA_HELPY)

Names and origin

Protein names

Recommended name:
    Protein recA
Alternative name(s):
    Recombinase A

Gene names

Name:	recA
Ordered Locus Names:	HP_0153

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage. HAMAP MF_00268 Deletion of this gene leads to the inability of the bacteria to perform homologous recombination, and markedly increases UV sensitivity. HAMAP MF_00268
Subcellular location	Cytoplasm By similarity.
Post-translational modification	The protein migrates as a 40 kDa protein in strains 69A and NCTC 11637. When overexpressed in E.coli a 38 kDa protein is made which is unable to complement the E.coli deletion mutant. It has been suggested this size difference is due to a post-translational modification. HAMAP MF_00268
Sequence similarities	Belongs to the recA family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA-dependent ATPase activity Inferred from electronic annotation. Source: HAMAP damaged DNA binding Inferred from electronic annotation. Source: HAMAP single-stranded DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Protein recA HAMAP MF_00268		PRO_0000122725
Regions
	Nucleotide binding	67 – 74	8	ATP By similarity
Natural variations
	Natural variant	40	1	S → A in strain: 69A and ATCC 53726.
	Natural variant	103	1	H → Y in strain: ATCC 53726.
	Natural variant	113	1	E → Q in strain: 69A.
	Natural variant	121	1	D → S in strain: ATCC 53726.
	Natural variant	125	1	Q → E in strain: ATCC 53726.
	Natural variant	203	1	M → T in strain: ATCC 53726.
	Natural variant	232	1	S → T in strain: 69A.
	Natural variant	319	1	D → N in strain: ATCC 53726.

Sequences

	Sequence		Length	Mass (Da)
	P42445-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: C8417BA54ACD27D8	FASTA	347	37,686
	10 20 30 40 50 60 MAIDEDKQKA ISLAIKQIDK VFGKGALVRL GDKQVEKIDS ISTGSLGLDL ALGIGGVPKG 70 80 90 100 110 120 RIIEIYGPES SGKTTLSLHI IAECQKNGGV CAFIDAEHAL DVHYAKRLGV DTENLLVSQP 130 140 150 160 170 180 DTGEQALEIL ETITRSGGID LVVVDSVAAL TPKAEIDGDM GDQHVGLQAR LMSHALRKIT 190 200 210 220 230 240 GVLHKMNTTL IFINQIRMKI GMMGYGSPET TTGGNALKFY ASVRIDIRRI ASLKQNEQHI 250 260 270 280 290 300 GNRAKAKVVK NKVAPPFREA EFDIMFGEGI SKEGEIIDYG VKLDIVDKSG AWLSYQDKKL 310 320 330 340 GQGRENAKAL LKEDKALADE ITLKIKESIG SNEEIMPLPD EPLEEME

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the Helicobacter pylori recA gene and involvement of the recA region in resistance to low pH." Thompson S.A., Blaser M.J. Infect. Immun. 63:2185-2193(1995) [PubMed: 7768597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 53726 / 84-183.
[2]	"Cloning of the Helicobacter pylori recA gene and functional characterization of its product." Schmitt W., Odenbreit S., Heuermann D., Haas R. Mol. Gen. Genet. 248:563-572(1995) [PubMed: 7476856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, SUGGESTION OF A POST-TRANSLATIONAL MODIFICATION. Strain: 69A and ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487.
[3]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z35478 Genomic DNA. Translation: CAA84615.1. U13756 Genomic DNA. Translation: AAC43379.1. AE000511 Genomic DNA. Translation: AAD07218.1.
PIR	S58683.
RefSeq	NP_206952.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	898827.
GenomeReviews	Gene locus HP_0153 in contig AE000511_GR.
KEGG	hpy:HP0153.
NMPDR	fig|85962.1.peg.150.
TIGR	HP_0153.
Phylogenomic databases
HOGENOM	HBG339889.
OMA	GRDNTIT.
Family and domain databases
HAMAP	MF_00268. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR013765. DNA_recomb/repair_RecA. IPR001553. DNA_recomb/repair_RecA/RadB. IPR020584. DNA_recomb/repair_RecA_CS. IPR020588. DNA_recomb_RecA/RadB_ATP-bd. IPR020587. RecA_monomer-monomer_interface. [Graphical view]
PANTHER	PTHR22942:SF1. RecA. 1 hit.
Pfam	PF00154. RecA. 1 hit. [Graphical view]
PRINTS	PR00142. RECA.
SMART	SM00382. AAA. 1 hit. [Graphical view]
PROSITE	PS00321. RECA_1. 1 hit. PS50162. RECA_2. 1 hit. PS50163. RECA_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RECA_HELPY

Accession

Primary (citable) accession number: P42445

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1997
Last modified:	December 15, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)