P42443 (RECA_DEIRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein RecA

Alternative name(s):
Recombinase A

Gene names

Name:	recA
Ordered Locus Names:	DR_2340

Organism

Deinococcus radiodurans

Taxonomic identifier

1299 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage. HAMAP MF_00268
Subcellular location	Cytoplasm By similarity HAMAP MF_00268.
Sequence similarities	Belongs to the RecA family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-dependent ATPase activity Inferred from electronic annotation. Source: InterPro single-stranded DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 363

363

Protein RecA HAMAP MF_00268

PRO_0000122700

Regions

Nucleotide binding

78 – 85

8

ATP By similarity

Natural variations

Natural variant

224

1

G → S in rec30; DNA-repair deficient.

Secondary structure

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363

Helix Strand Turn

Details...

Helix

17 – 33

17

Beta strand

37 – 40

4

Helix

57 – 62

6

Beta strand

64 – 69

6

Beta strand

72 – 79

8

Helix

84 – 97

14

Beta strand

102 – 108

7

Helix

113 – 118

6

Helix

123 – 125

3

Beta strand

127 – 129

3

Helix

134 – 145

12

Turn

146 – 148

3

Beta strand

151 – 156

6

Turn

158 – 160

3

Helix

178 – 194

17

Turn

195 – 197

3

Beta strand

200 – 206

7

Helix

224 – 230

7

Beta strand

232 – 241

10

Beta strand

252 – 265

14

Beta strand

270 – 276

7

Turn

277 – 279

3

Helix

283 – 293

11

Beta strand

296 – 300

5

Beta strand

303 – 314

12

Helix

315 – 322

8

Helix

326 – 340

15

Sequences

	Sequence		Length	Mass (Da)
	P42443 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: DEF379CF0EF59D74	FASTA	363	38,145
	10 20 30 40 50 60 MSKDATKEIS APTDAKERSK AIETAMSQIE KAFGKGSIMK LGAESKLDVQ VVSTGSLSLD 70 80 90 100 110 120 LALGVGGIPR GRITEIYGPE SGGKTTLALA IVAQAQKAGG TCAFIDAEHA LDPVYARALG 130 140 150 160 170 180 VNTDELLVSQ PDNGEQALEI MELLVRSGAI DVVVVDSVAA LTPRAEIEGD MGDSLPGLQA 190 200 210 220 230 240 RLMSQALRKL TAILSKTGTA AIFINQVREK IGVMYGNPET TTGGRALKFY ASVRLDVRKI 250 260 270 280 290 300 GQPTKVGNDA VANTVKIKTV KNKVAAPFKE VELALVYGKG FDQLSDLVGL AADMDIIKKA 310 320 330 340 350 360 GSFYSYGDER IGQGKEKTIA YIAERPEMEQ EIRDRVMAAI RAGNAGEAPA LAPAPAAPEA AEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing, targeted mutagenesis and expression of a recA gene required for the extreme radioresistance of Deinococcus radiodurans." Gutman P.D., Carroll J.D., Masters C.I., Minton K.W. Gene 141:31-37(1994) [PubMed: 8163172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
[2]	Carroll J.D. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Molecular analysis of the Deinococcus radiodurans recA locus and identification of a mutation site in a DNA repair-deficient mutant, rec30." Narumi I., Satoh K., Kikuchi M., Funayama T., Kitayama S., Yanagisawa T., Watanabe H., Yamamoto K. Mutat. Res. 435:233-243(1999) [PubMed: 10606814] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANT REC30. Strain: KD8301.
[4]	"Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1." White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D., Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L., Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M., Vamathevan J.J., Lam P. , McDonald L.A., Utterback T.R., Zalewski C., Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D., Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Science 286:1571-1577(1999) [PubMed: 10567266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U01876 Genomic DNA. Translation: AAC33148.1.
AB005471 Genomic DNA. Translation: BAA21330.1.
AE000513 Genomic DNA. Translation: AAF11887.1.

PIR

C75285.

RefSeq

NP_296061.1. NC_001263.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XP8	X-ray	2.50	A	1-363	[»]

ProteinModelPortal

P42443.

SMR

P42443. Positions 15-341.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1798669.

GenomeReviews

Gene locus DR_2340 in contig AE000513_GR.

KEGG

dra:DR_2340.

NMPDR

fig|243230.1.peg.2520.

PATRIC

21632388. VBIDeiRad64572_2571.

TIGR

DR_2340.

Phylogenomic databases

HOGENOM

HBG339889.

OMA

MKIDANK.

PhylomeDB

P42443.

ProtClustDB

PRK09354.

Enzyme and pathway databases

BioCyc

DRAD243230:DR_2340-MONOMER.

Family and domain databases

HAMAP

MF_00268. RecA.
[Tree]

InterPro

IPR003593. ATPase_AAA+_core.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR020588. DNA_recomb_RecA/RadB_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]

Gene3D

G3DSA:3.30.250.10. G3DSA:3.30.250.10. 1 hit.

KO

K03553.

PANTHER

PTHR22942:SF1. RecA. 1 hit.

Pfam

PF00154. RecA. 1 hit.
[Graphical view]

PRINTS

PR00142. RECA.

SMART

SM00382. AAA. 1 hit.
[Graphical view]

SUPFAM

SSF54752. SSF54752. 1 hit.

TIGRFAMs

TIGR02012. Tigrfam_recA. 1 hit.

PROSITE

PS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RECA_DEIRA

Accession

Primary (citable) accession number: P42443
Secondary accession number(s): O32510

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program