P42439 (EFTU_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Tu
Short name=EF-Tu

Gene names

Name:	tuf
Ordered Locus Names:	Cgl0497, cg0587

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118_B
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00118_B
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00118_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Elongation factor Tu HAMAP-Rule MF_00118_B		PRO_0000091316
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	83 – 87	5	GTP By similarity
	Nucleotide binding	138 – 141	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42439 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: F46333EC113AB5FD	FASTA	396	43,852
	10 20 30 40 50 60 MAKAKFERTK PHVNIGTIGH VDHGKTTTTA AITKVLADTY PELNEAFAFD SIDKAPEEKE 70 80 90 100 110 120 RGITINISHV EYQTEKRHYA HVDAPGHADY IKNMITGAAQ MDGAILVVAA TDGPMPQTRE 130 140 150 160 170 180 HVLLARQVGV PYILVALNKC DMVEDEEIIE LVEMEVRELL AEQDYDEEAP IVHISALKAL 190 200 210 220 230 240 EGDEKWGKQI LELMQACDDN IPDPVRETDK PFLMPIEDIF TITGRGTVVT GRVERGTLNV 250 260 270 280 290 300 NDDVDIIGIK EKSTSTTVTG IEMFRKLLDS AEAGDNCGLL LRGIKREDVE RGQVIVKPGA 310 320 330 340 350 360 YTPHTEFEGS VYVLSKDEGG RHTPFFDNYR PQFYFRTTDV TGVVKLPEGT EMVMPGDNVD 370 380 390 MSVTLIQPVA MDEGLRFAIR EGSRTVGAGR VTKIIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phylogenetic relationships of Bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit genes." Ludwig W., Neumaier J., Klugbauer N., Brockmann E., Roller C., Klugbauer S., Reetz K., Schachtner I., Ludvigsen A., Bachleitner M., Fischer U., Schleifer K.H. Antonie Van Leeuwenhoek 64:285-305(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[2]	"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77034 Genomic DNA. Translation: CAA54323.1. BA000036 Genomic DNA. Translation: BAB97890.1. BX927149 Genomic DNA. Translation: CAF19210.1.
RefSeq	NP_599741.1. NC_003450.3. YP_224796.1. NC_006958.1.
3D structure databases
ProteinModelPortal	P42439.
SMR	P42439. Positions 2-395.
ModBase	Search...
Protein-protein interaction databases
STRING	196627.cg0587.
2D gel databases
World-2DPAGE	0001:P42439.
Proteomic databases
PRIDE	P42439.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB97890; BAB97890; BAB97890. CAF19210; CAF19210; cg0587.
GeneID	1021503. 3343112.
KEGG	cgb:cg0587. cgl:NCgl0480.
PATRIC	21493060. VBICorGlu203724_0496.
Phylogenomic databases
eggNOG	COG0050.
HOGENOM	HOG000229290.
KO	K02358.
OMA	CEFVGYN.
ProtClustDB	PRK00049.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF52540. SSF52540. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU_CORGL

Accession

Primary (citable) accession number: P42439

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program