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Protein

Uroporphyrinogen-III C-methyltransferase

Gene

nasF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

GO - Molecular functioni

  1. precorrin-2 dehydrogenase activity Source: InterPro
  2. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-EC
  3. uroporphyrinogen-III synthase activity Source: InterPro

GO - Biological processi

  1. porphyrin-containing compound biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Porphyrin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU03280-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Gene namesi
Name:nasF
Synonyms:nasBE
Ordered Locus Names:BSU03280
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU03280. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Uroporphyrinogen-III C-methyltransferasePRO_0000150384Add
BLAST

Proteomic databases

PaxDbiP42437.

Expressioni

Inductioni

Positively regulated by TnrA under nitrogen-limited conditions.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU03280.

Structurei

3D structure databases

ProteinModelPortaliP42437.
SMRiP42437. Positions 1-250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG1587.
HOGENOMiHOG000290968.
InParanoidiP42437.
KOiK13542.
OMAiWKERKPL.
OrthoDBiEOG6BGNXZ.
PhylomeDBiP42437.

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR003754. 4pyrrol_synth_uPrphyn_synth.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53790. SSF53790. 1 hit.
SSF69618. SSF69618. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42437-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIMKNGIVYF VGAGPGDPGL LTIKGKQALK EADVILYDRL ANPKLLEFAS
60 70 80 90 100
PDCQFIYCGK LPNRHFMKQK EINALLVEKA LNGLTVVRLK GGDPSVFGRV
110 120 130 140 150
GEEADALHEH GIRYEMVPGI TSGIAAPLYA GIPVTHRDFA SSFAMITAHD
160 170 180 190 200
KSLKGTPNLD WEGLARSVQT LVFYMGVKNL SYICQQLISY GKSPSVPVIV
210 220 230 240 250
IQWGTWGRQR SVKGTLENIQ QKVQEHQITN PAIIVIGDIV NFQTHSWFES
260 270 280 290 300
KPLIGRHLMV VTHGEDEDPL ADKLRDSGAD LIEWPKWRTE NMPVNEEILR
310 320 330 340 350
KIGTFEDVFF TSRRAVCEFF RALASQKIDI RQLTAKLSAA SEQAKTELEK
360 370 380 390 400
RGFLVTAIQP DSEKRLVVGS RHAVENMQKH ESCSFYITHE NVIDDRFTHM
410 420 430 440 450
IQRTISESPL HMVICPNKLS VQQLINGGEQ IGILPEPSAS RPPIVCIGDD
460 470 480
SAAGIYGFTA VQEQDELLAF IHNQHAEKKL LHT
Length:483
Mass (Da):53,869
Last modified:November 1, 1995 - v1
Checksum:i49752620FA96B74C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30689 Genomic DNA. Translation: BAA06356.1.
D50453 Genomic DNA. Translation: BAA08962.1.
AL009126 Genomic DNA. Translation: CAB12122.1.
PIRiI40031.
RefSeqiNP_388210.1. NC_000964.3.
WP_003234641.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB12122; CAB12122; BSU03280.
GeneIDi938324.
KEGGibsu:BSU03280.
PATRICi18972215. VBIBacSub10457_0336.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30689 Genomic DNA. Translation: BAA06356.1.
D50453 Genomic DNA. Translation: BAA08962.1.
AL009126 Genomic DNA. Translation: CAB12122.1.
PIRiI40031.
RefSeqiNP_388210.1. NC_000964.3.
WP_003234641.1. NC_000964.3.

3D structure databases

ProteinModelPortaliP42437.
SMRiP42437. Positions 1-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU03280.

Proteomic databases

PaxDbiP42437.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12122; CAB12122; BSU03280.
GeneIDi938324.
KEGGibsu:BSU03280.
PATRICi18972215. VBIBacSub10457_0336.

Organism-specific databases

GenoListiBSU03280. [Micado]

Phylogenomic databases

eggNOGiCOG1587.
HOGENOMiHOG000290968.
InParanoidiP42437.
KOiK13542.
OMAiWKERKPL.
OrthoDBiEOG6BGNXZ.
PhylomeDBiP42437.

Enzyme and pathway databases

BioCyciBSUB:BSU03280-MONOMER.

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR003754. 4pyrrol_synth_uPrphyn_synth.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53790. SSF53790. 1 hit.
SSF69618. SSF69618. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis."
    Ogawa K., Akagawa E., Yamane K., Sun Z.-W., Lacelle M., Zuber P., Nakano M.M.
    J. Bacteriol. 177:1409-1413(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes."
    Yamane K., Kumano M., Kurita K.
    Microbiology 142:3047-3056(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Purification and in vitro activities of the Bacillus subtilis TnrA transcription factor."
    Wray L.V. Jr., Zalieckas J.M., Fisher S.H.
    J. Mol. Biol. 300:29-40(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY TNRA.
    Strain: 168.
  5. "Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box."
    Yoshida K., Yamaguchi H., Kinehara M., Ohki Y.-H., Nakaura Y., Fujita Y.
    Mol. Microbiol. 49:157-165(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY TNRA.

Entry informationi

Entry nameiNASF_BACSU
AccessioniPrimary (citable) accession number: P42437
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.