ID   NASE_BACSU              Reviewed;         106 AA.
AC   P42436;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   16-JUN-2009, entry version 67.
DE   RecName: Full=Assimilatory nitrite reductase [NAD(P)H] small subunit;
DE            EC=1.7.1.4;
GN   Name=nasE; Synonyms=nasBD, nirD; OrderedLocusNames=BSU03290;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=95173124; PubMed=7868621;
RA   Ogawa K., Akagawa E., Yamane K., Sun Z.-W., Lacelle M., Zuber P.,
RA   Nakano M.M.;
RT   "The nasB operon and nasA gene are required for nitrate/nitrite
RT   assimilation in Bacillus subtilis.";
RL   J. Bacteriol. 177:1409-1413(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=97124189; PubMed=8969502;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification
RT   of 113 genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   REGULATION BY TNRA.
RC   STRAIN=168;
RX   PubMed=10864496; DOI=10.1006/jmbi.2000.3846;
RA   Wray L.V. Jr., Zalieckas J.M., Fisher S.H.;
RT   "Purification and in vitro activities of the Bacillus subtilis TnrA
RT   transcription factor.";
RL   J. Mol. Biol. 300:29-40(2000).
RN   [5]
RP   REGULATION.
RC   STRAIN=168;
RX   PubMed=11698370; DOI=10.1128/JB.183.23.6815-6821.2001;
RA   Marino M., Cruz Ramos H., Hoffmann T., Glaser P., Jahn D.;
RT   "Modulation of anaerobic energy metabolism of Bacillus subtilis by
RT   arfM (ywiD).";
RL   J. Bacteriol. 183:6815-6821(2001).
CC   -!- FUNCTION: Required for nitrite assimilation. Required for activity
CC       of the reductase (By similarity).
CC   -!- CATALYTIC ACTIVITY: Ammonium hydroxide + 3 NAD(P)(+) + H(2)O =
CC       nitrite + 3 NAD(P)H.
CC   -!- COFACTOR: Binds 1 2Fe-2S cluster.
CC   -!- SUBUNIT: Associates with nasD (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- INDUCTION: Positively regulated by tnrA under conditions of
CC       nitrogen limitation. Induced by arfM.
CC   -!- SIMILARITY: Contains 1 Rieske domain.
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DR   EMBL; D30689; BAA06355.1; -; Genomic_DNA.
DR   EMBL; D50453; BAA08963.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12123.1; -; Genomic_DNA.
DR   PIR; I40030; I40030.
DR   RefSeq; NP_388211.1; -.
DR   GeneID; 938331; -.
DR   GenomeReviews; AL009126_GR; BSU03290.
DR   KEGG; bsu:BSU03290; -.
DR   NMPDR; fig|224308.1.peg.330; -.
DR   SubtiList; BG11097; nasE.
DR   HOGENOM; P42436; -.
DR   OMA; P42436; ENEVFAI.
DR   BioCyc; BSUB224308:BSU0330-MON; -.
DR   BRENDA; 1.7.1.4; 150.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008942; F:nitrite reductase [NAD(P)H] activity; IEA:EC.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR012748; Nitri_red_NirD.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   Gene3D; G3DSA:2.102.10.10; Rieske_reg; 1.
DR   Pfam; PF00355; Rieske; 1.
DR   TIGRFAMs; TIGR02378; nirD_assim_sml; 1.
DR   PROSITE; PS51296; RIESKE; 1.
PE   2: Evidence at transcript level;
KW   2Fe-2S; Complete proteome; Cytoplasm; Iron; Iron-sulfur;
KW   Metal-binding; NAD; Nitrate assimilation; Oxidoreductase.
FT   CHAIN         1    106       Assimilatory nitrite reductase [NAD(P)H]
FT                                small subunit.
FT                                /FTId=PRO_0000096737.
FT   DOMAIN        8    104       Rieske.
FT   METAL        49     49       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL        51     51       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
FT   METAL        68     68       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL        71     71       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
SQ   SEQUENCE   106 AA;  11895 MW;  26195A275119523D CRC64;
     MVNKDVTKVC IGKIEELPEQ LGKTVYIEDK ELAVFKLSDG SIRAIENRCP HKGGVLAEGI
     VSGQYVFCPM HDWKISLEDG IVQEPDHGCV KTYETLIEGE HVYLVY
//