Reviewed,
UniProtKB/Swiss-Prot P42435 (NASD_BACSU)
Last modified
November 25, 2008.
Version 83.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Nitrite reductase [NAD(P)H] EC=1.7.1.4 | ||||||
| Gene names |
| ||||||
| Organism | Bacillus subtilis [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 1423 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 805 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Required for nitrite assimilation. |
| Catalytic activity | Ammonium hydroxide + 3 NAD(P)(+) + H(2)O = nitrite + 3 NAD(P)H. |
| Cofactor | Binds 1 siroheme per subunit. Binds 1 4Fe-4S cluster per subunit. FAD. |
| Pathway | |
| Subunit structure | Homodimer By similarity. |
| Induction | Positively regulated by tnrA under conditions of nitrogen limitation. Induced by arfM. |
| Sequence similarities | Belongs to the nitrite and sulfite reductase 4Fe-4S domain family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Nitrate assimilation |
| Ligand | 4Fe-4S FAD Flavoprotein Heme Iron Iron-sulfur Metal-binding NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | nitrate assimilation Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FAD bindingInferred from electronic annotation. Source: InterPro NADP bindingInferred from electronic annotation. Source: InterPro electron carrier activityInferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW nitrite reductase [NAD(P)H] activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 805 | 805 | Nitrite reductase [NAD(P)H] | PRO_0000199961 | |||||
Regions | |||||||||
| Nucleotide binding | 43 – 79 | 37 | FAD Potential | ||||||
| Nucleotide binding | 193 – 223 | 31 | NAD or NADP Potential | ||||||
Sites | |||||||||
| Metal binding | 635 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 641 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 675 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 679 | 1 | Iron (siroheme axial ligand) By similarity | ||||||
| Metal binding | 679 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis." Ogawa K., Akagawa E., Yamane K., Sun Z.-W., Lacelle M., Zuber P., Nakano M.M. J. Bacteriol. 177:1409-1413(1995) [PubMed: 7868621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [2] | "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes." Yamane K., Kumano M., Kurita K. Microbiology 142:3047-3056(1996) [PubMed: 8969502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [3] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [4] | "Purification and in vitro activities of the Bacillus subtilis TnrA transcription factor." Wray L.V. Jr., Zalieckas J.M., Fisher S.H. J. Mol. Biol. 300:29-40(2000) [PubMed: 10864496] [Abstract] Cited for: REGULATION BY TNRA. Strain: 168. |
| [5] | "Modulation of anaerobic energy metabolism of Bacillus subtilis by arfM (ywiD)." Marino M., Cruz Ramos H., Hoffmann T., Glaser P., Jahn D. J. Bacteriol. 183:6815-6821(2001) [PubMed: 11698370] [Abstract] Cited for: REGULATION. Strain: 168. |
Cross-references
Sequence databases | |
|---|---|
| D30689 Genomic DNA. Translation: BAA06354.1. D50453 Genomic DNA. Translation: BAA08964.1. Z99105 Genomic DNA. Translation: CAB12124.1. | |
| PIR | I40029. |
| RefSeq | NP_388212.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 938329. |
| GenomeReviews | Gene locus BSU03300 in contig AL009126_GR. |
| KEGG | bsu:BSU03300. |
| NMPDR | fig|224308.1.peg.331. |
Organism-specific databases | |
| SubtiList | BG11096. nasD. [Micado] |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P42435. |
Enzyme and pathway databases | |
| BioCyc | BSUB224308:BSU0331-MON. |
Family and domain databases | |
| InterPro | IPR007419. BFD_Fer2_bd. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR012744. Nitri_red_NirB. IPR017121. Nitrite_Rdtase_lsu. IPR001100. Pyr_nuc-diS_OxRdtase. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view] |
| Pfam | PF04324. Fer2_BFD. 2 hits. PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 1 hit. PF00070. Pyr_redox. 2 hits. PF07992. Pyr_redox_2. 1 hit. [Graphical view] |
| PIRSF | PIRSF037149. NirB. 1 hit. |
| PRINTS | PR00368. FADPNR. PR00411. PNDRDTASEI. PR00397. SIROHAEM. |
| ProDom | PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR02374. nitri_red_nirB. 1 hit. |
| PROSITE | PS00365. NIR_SIR. False negative. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| LinkHub | P42435. |
Entry information
| Entry name | NASD_BACSU | ||||||||
| Accession | Primary (citable) accession number: P42435 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


