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Protein

Nitrite reductase [NAD(P)H]

Gene

nasD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for nitrite assimilation.1 Publication

Catalytic activityi

Ammonia + 3 NAD(P)+ + 2 H2O = nitrite + 3 NAD(P)H.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: nitrate reduction (assimilation)

This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi635 – 6351Iron-sulfur (4Fe-4S)By similarity
Metal bindingi641 – 6411Iron-sulfur (4Fe-4S)By similarity
Metal bindingi675 – 6751Iron-sulfur (4Fe-4S)By similarity
Metal bindingi679 – 6791Iron (siroheme axial ligand)By similarity
Metal bindingi679 – 6791Iron-sulfur (4Fe-4S)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 7937FADSequence analysisAdd
BLAST
Nucleotide bindingi193 – 22331NAD or NADPSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

4Fe-4S, FAD, Flavoprotein, Heme, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciBSUB:BSU03300-MONOMER.
UniPathwayiUPA00653.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrite reductase [NAD(P)H] (EC:1.7.1.4)
Gene namesi
Name:nasD
Synonyms:nasBC, nirB
Ordered Locus Names:BSU03300
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 805805Nitrite reductase [NAD(P)H]PRO_0000199961Add
BLAST

Proteomic databases

PaxDbiP42435.

Expressioni

Inductioni

Positively regulated by TnrA under nitrogen-limited conditions. Induced by ArfM.3 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001838.

Structurei

3D structure databases

ProteinModelPortaliP42435.
SMRiP42435. Positions 2-389.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108IFG. Bacteria.
COG1251. LUCA.
HOGENOMiHOG000196165.
InParanoidiP42435.
KOiK00362.
OMAiYREQARY.
PhylomeDBiP42435.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
IPR023753. FAD/NAD-binding_dom.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR012744. Nitri_red_NirB.
IPR017121. Nitrite_Rdtase_lsu.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF04324. Fer2_BFD. 2 hits.
PF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PIRSFiPIRSF037149. NirB. 1 hit.
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF51905. SSF51905. 2 hits.
SSF55124. SSF55124. 1 hit.
TIGRFAMsiTIGR02374. nitri_red_nirB. 1 hit.

Sequencei

Sequence statusi: Complete.

P42435-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKQLVLVG NGMAGVRAIE EILSVAKDEF QITIFGAEPH PNYNRILLSK
60 70 80 90 100
VLQGDTDIKD ITLNDWDWYE ENNIQLYTNE TVIKVDTENK TVITDADRIQ
110 120 130 140 150
PYDELILATG SVPFILPIPG ADKKGVTAFR DIKDTDTMLA ASKQYKKAAV
160 170 180 190 200
IGGGLLGLEA ARGLLNLGMD VSVIHLAPFL MERQLDATAG RLLQNELEKQ
210 220 230 240 250
GMTFLLEKQT EEIVGDDRVE GLRFKDGTSI EADLVVMAVG IRPNTTLGAE
260 270 280 290 300
SGIPVNRGII VNDYMQTEIP HIYAVGECAE HRGIAYGLVA PLYEQAKVLA
310 320 330 340 350
KHMCGIETKP YEGSVLSTQL KVSGVEVFSA GDFNESEEKK AIKVFDEQDG
360 370 380 390 400
IYKKIVLRGN QIVGAVLFGD SSEGNRLFSM IQKEADISET SKISILQPLS
410 420 430 440 450
QEAGTSITAA MSDDEIICGC NGVSKGAIIQ AIQEKGCSST DEIKACTGAS
460 470 480 490 500
RSCGGCKPLV EEILQHTLGS DFDASAQKEA ICGCTTLSRD EVVEEIKAKG
510 520 530 540 550
LSHTREVMNV LGWKTPEGCS KCRPALNYYL GMINPTKYED DRTSRFVNER
560 570 580 590 600
MHANIQKDGT YSVVPRMYGG VTNSTDLRKI ADVVDKYEIP LVKMTGGQRI
610 620 630 640 650
DLIGVKKEDL PKVWEDLDMP SGYAYGKTLR TVKTCVGEQF CRFGTQDSMA
660 670 680 690 700
LGIALEKKFE GLNTPHKVKM AVSACPRNCA ESGIKDLGVV GIDGGWELYV
710 720 730 740 750
GGNGGTHLRA GDLLMKVKTN EEVLEYAGAY LQYYRETANY LERTSAWLER
760 770 780 790 800
VGLSHVQSVL NDPEKRQELN GRMNETLSVH KDPWKDFLED KQTSKELFEN

VVTTS
Length:805
Mass (Da):88,432
Last modified:November 1, 1995 - v1
Checksum:i5ED3295AF3E0CA29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30689 Genomic DNA. Translation: BAA06354.1.
D50453 Genomic DNA. Translation: BAA08964.1.
AL009126 Genomic DNA. Translation: CAB12124.1.
PIRiI40029.
RefSeqiNP_388212.1. NC_000964.3.
WP_003234637.1. NZ_JNCM01000030.1.

Genome annotation databases

EnsemblBacteriaiCAB12124; CAB12124; BSU03300.
GeneIDi938329.
KEGGibsu:BSU03300.
PATRICi18972219. VBIBacSub10457_0338.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30689 Genomic DNA. Translation: BAA06354.1.
D50453 Genomic DNA. Translation: BAA08964.1.
AL009126 Genomic DNA. Translation: CAB12124.1.
PIRiI40029.
RefSeqiNP_388212.1. NC_000964.3.
WP_003234637.1. NZ_JNCM01000030.1.

3D structure databases

ProteinModelPortaliP42435.
SMRiP42435. Positions 2-389.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001838.

Proteomic databases

PaxDbiP42435.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12124; CAB12124; BSU03300.
GeneIDi938329.
KEGGibsu:BSU03300.
PATRICi18972219. VBIBacSub10457_0338.

Phylogenomic databases

eggNOGiENOG4108IFG. Bacteria.
COG1251. LUCA.
HOGENOMiHOG000196165.
InParanoidiP42435.
KOiK00362.
OMAiYREQARY.
PhylomeDBiP42435.

Enzyme and pathway databases

UniPathwayiUPA00653.
BioCyciBSUB:BSU03300-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
IPR023753. FAD/NAD-binding_dom.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR012744. Nitri_red_NirB.
IPR017121. Nitrite_Rdtase_lsu.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF04324. Fer2_BFD. 2 hits.
PF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PIRSFiPIRSF037149. NirB. 1 hit.
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF51905. SSF51905. 2 hits.
SSF55124. SSF55124. 1 hit.
TIGRFAMsiTIGR02374. nitri_red_nirB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNASD_BACSU
AccessioniPrimary (citable) accession number: P42435
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.