Reviewed, UniProtKB/Swiss-Prot P42435 (NASD_BACSU)

Names and origin

Protein names

Recommended name:
    Nitrite reductase [NAD(P)H]
    EC=1.7.1.4

Gene names

Name:	nasD
Synonyms:	nasBC, nirB
Ordered Locus Names:	BSU03300

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	805 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Required for nitrite assimilation. Ref.1
Catalytic activity	Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H.
Cofactor	Binds 1 siroheme per subunit. Binds 1 4Fe-4S cluster per subunit. FAD.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation).
Subunit structure	Homodimer By similarity.
Induction	Positively regulated by tnrA under nitrogen-limited conditions. Induced by arfM. Ref.4 Ref.5 Ref.6
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	4Fe-4S FAD Flavoprotein Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro nitrite reductase [NAD(P)H] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 805	805	Nitrite reductase [NAD(P)H]		PRO_0000199961
Regions
	Nucleotide binding	43 – 79	37	FAD Potential
	Nucleotide binding	193 – 223	31	NAD or NADP Potential
Sites
	Metal binding	635	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	641	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	675	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	679	1	Iron (siroheme axial ligand) By similarity
	Metal binding	679	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42435-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5ED3295AF3E0CA29	FASTA	805	88,432
	10 20 30 40 50 60 MGKKQLVLVG NGMAGVRAIE EILSVAKDEF QITIFGAEPH PNYNRILLSK VLQGDTDIKD 70 80 90 100 110 120 ITLNDWDWYE ENNIQLYTNE TVIKVDTENK TVITDADRIQ PYDELILATG SVPFILPIPG 130 140 150 160 170 180 ADKKGVTAFR DIKDTDTMLA ASKQYKKAAV IGGGLLGLEA ARGLLNLGMD VSVIHLAPFL 190 200 210 220 230 240 MERQLDATAG RLLQNELEKQ GMTFLLEKQT EEIVGDDRVE GLRFKDGTSI EADLVVMAVG 250 260 270 280 290 300 IRPNTTLGAE SGIPVNRGII VNDYMQTEIP HIYAVGECAE HRGIAYGLVA PLYEQAKVLA 310 320 330 340 350 360 KHMCGIETKP YEGSVLSTQL KVSGVEVFSA GDFNESEEKK AIKVFDEQDG IYKKIVLRGN 370 380 390 400 410 420 QIVGAVLFGD SSEGNRLFSM IQKEADISET SKISILQPLS QEAGTSITAA MSDDEIICGC 430 440 450 460 470 480 NGVSKGAIIQ AIQEKGCSST DEIKACTGAS RSCGGCKPLV EEILQHTLGS DFDASAQKEA 490 500 510 520 530 540 ICGCTTLSRD EVVEEIKAKG LSHTREVMNV LGWKTPEGCS KCRPALNYYL GMINPTKYED 550 560 570 580 590 600 DRTSRFVNER MHANIQKDGT YSVVPRMYGG VTNSTDLRKI ADVVDKYEIP LVKMTGGQRI 610 620 630 640 650 660 DLIGVKKEDL PKVWEDLDMP SGYAYGKTLR TVKTCVGEQF CRFGTQDSMA LGIALEKKFE 670 680 690 700 710 720 GLNTPHKVKM AVSACPRNCA ESGIKDLGVV GIDGGWELYV GGNGGTHLRA GDLLMKVKTN 730 740 750 760 770 780 EEVLEYAGAY LQYYRETANY LERTSAWLER VGLSHVQSVL NDPEKRQELN GRMNETLSVH 790 800 KDPWKDFLED KQTSKELFEN VVTTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis." Ogawa K., Akagawa E., Yamane K., Sun Z.-W., Lacelle M., Zuber P., Nakano M.M. J. Bacteriol. 177:1409-1413(1995) [PubMed: 7868621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: 168.
[2]	"The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes." Yamane K., Kumano M., Kurita K. Microbiology 142:3047-3056(1996) [PubMed: 8969502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Purification and in vitro activities of the Bacillus subtilis TnrA transcription factor." Wray L.V. Jr., Zalieckas J.M., Fisher S.H. J. Mol. Biol. 300:29-40(2000) [PubMed: 10864496] [Abstract] Cited for: INDUCTION BY TNRA. Strain: 168.
[5]	"Modulation of anaerobic energy metabolism of Bacillus subtilis by arfM (ywiD)." Marino M., Cruz Ramos H., Hoffmann T., Glaser P., Jahn D. J. Bacteriol. 183:6815-6821(2001) [PubMed: 11698370] [Abstract] Cited for: INDUCTION BY ARFM. Strain: 168.
[6]	"Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box." Yoshida K., Yamaguchi H., Kinehara M., Ohki Y.-H., Nakaura Y., Fujita Y. Mol. Microbiol. 49:157-165(2003) [PubMed: 12823818] [Abstract] Cited for: INDUCTION BY TNRA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D30689 Genomic DNA. Translation: BAA06354.1. D50453 Genomic DNA. Translation: BAA08964.1. AL009126 Genomic DNA. Translation: CAB12124.1.
PIR	I40029.
RefSeq	NP_388212.1.
3D structure databases
SMR	P42435. Positions 3-388, 5-470, 538-759, 568-775.
ModBase	Search...
Genome annotation databases
GeneID	938329.
GenomeReviews	Gene locus BSU03300 in contig AL009126_GR.
KEGG	bsu:BSU03300.
NMPDR	fig|224308.1.peg.331.
Organism-specific databases
SubtiList	BG11096. nasD. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285339.
OMA	YLISTWP.
PhylomeDB	P42435.
Enzyme and pathway databases
BRENDA	1.7.1.4. 150.
Family and domain databases
InterPro	IPR007419. BFD-like_2Fe2S-bd_dom. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR012744. Nitri_red_NirB. IPR017121. Nitrite_Rdtase_lsu. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Pfam	PF04324. Fer2_BFD. 2 hits. PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 1 hit. PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PIRSF	PIRSF037149. NirB. 1 hit.
PRINTS	PR00368. FADPNR. PR00397. SIROHAEM.
TIGRFAMs	TIGR02374. nitri_red_nirB. 1 hit.
PROSITE	PS00365. NIR_SIR. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NASD_BACSU

Accession

Primary (citable) accession number: P42435

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)