P42435 (NASD_BACSU) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 112.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Nitrite reductase [NAD(P)H] EC=1.7.1.4 | ||||||
| Gene names |
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| Organism | Bacillus subtilis | ||||||
| Taxonomic identifier | 1423 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 805 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Required for nitrite assimilation. Ref.1 |
| Catalytic activity | Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H. |
| Cofactor | Binds 1 siroheme per subunit. Binds 1 4Fe-4S cluster per subunit. FAD. |
| Pathway | |
| Subunit structure | Homodimer By similarity. |
| Induction | Positively regulated by TnrA under nitrogen-limited conditions. Induced by ArfM. Ref.4 Ref.5 Ref.6 |
| Sequence similarities | Belongs to the nitrite and sulfite reductase 4Fe-4S domain family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Nitrate assimilation |
| Ligand | 4Fe-4S FAD Flavoprotein Heme Iron Iron-sulfur Metal-binding NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP bindingInferred from electronic annotation. Source: InterPro flavin adenine dinucleotide bindingInferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro nitrite reductase [NAD(P)H] activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 805 | 805 | Nitrite reductase [NAD(P)H] | PRO_0000199961 | |||||
Regions | |||||||||
| Nucleotide binding | 43 – 79 | 37 | FAD Potential | ||||||
| Nucleotide binding | 193 – 223 | 31 | NAD or NADP Potential | ||||||
Sites | |||||||||
| Metal binding | 635 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 641 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 675 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 679 | 1 | Iron (siroheme axial ligand) By similarity | ||||||
| Metal binding | 679 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis." Ogawa K., Akagawa E., Yamane K., Sun Z.-W., Lacelle M., Zuber P., Nakano M.M. J. Bacteriol. 177:1409-1413(1995) [PubMed: 7868621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: 168. |
| [2] | "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes." Yamane K., Kumano M., Kurita K. Microbiology 142:3047-3056(1996) [PubMed: 8969502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [3] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [4] | "Purification and in vitro activities of the Bacillus subtilis TnrA transcription factor." Wray L.V. Jr., Zalieckas J.M., Fisher S.H. J. Mol. Biol. 300:29-40(2000) [PubMed: 10864496] [Abstract] Cited for: INDUCTION BY TNRA. Strain: 168. |
| [5] | "Modulation of anaerobic energy metabolism of Bacillus subtilis by arfM (ywiD)." Marino M., Cruz Ramos H., Hoffmann T., Glaser P., Jahn D. J. Bacteriol. 183:6815-6821(2001) [PubMed: 11698370] [Abstract] Cited for: INDUCTION BY ARFM. Strain: 168. |
| [6] | "Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box." Yoshida K., Yamaguchi H., Kinehara M., Ohki Y.-H., Nakaura Y., Fujita Y. Mol. Microbiol. 49:157-165(2003) [PubMed: 12823818] [Abstract] Cited for: INDUCTION BY TNRA. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D30689 Genomic DNA. Translation: BAA06354.1. D50453 Genomic DNA. Translation: BAA08964.1. AL009126 Genomic DNA. Translation: CAB12124.1. |
| PIR | I40029. |
| RefSeq | NP_388212.1. NC_000964.3. |
3D structure databases | |
| ProteinModelPortal | P42435. |
| SMR | P42435. Positions 2-389. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBBACT00000002961; EBBACP00000002961; EBBACG00000002955. |
| GeneID | 938329. |
| GenomeReviews | Gene locus BSU03300 in contig AL009126_GR. |
| KEGG | bsu:BSU03300. |
| NMPDR | fig|224308.1.peg.331. |
| PATRIC | 18972219. VBIBacSub10457_0338. |
Organism-specific databases | |
| GenoList | BSU03300. [Micado] |
Phylogenomic databases | |
| GeneTree | EBGT00070000031876. |
| HOGENOM | HBG285339. |
| OMA | YLISTWP. |
| PhylomeDB | P42435. |
| ProtClustDB | CLSK872770. |
Enzyme and pathway databases | |
| BioCyc | BSUB:BSU03300-MONOMER. |
Family and domain databases | |
| InterPro | IPR007419. BFD-like_2Fe2S-bd_dom. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR012744. Nitri_red_NirB. IPR017121. Nitrite_Rdtase_lsu. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.90.480.10. G3DSA:3.90.480.10. 1 hit. |
| KO | K00362. |
| Pfam | PF04324. Fer2_BFD. 2 hits. PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 1 hit. PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 2 hits. [Graphical view] |
| PIRSF | PIRSF037149. NirB. 1 hit. |
| PRINTS | PR00368. FADPNR. PR00397. SIROHAEM. |
| SUPFAM | SSF55124. NiR_SiRalpha_1/3. 1 hit. |
| TIGRFAMs | TIGR02374. Nitri_red_nirB. 1 hit. |
| PROSITE | PS00365. NIR_SIR. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NASD_BACSU | ||||||||
| Accession | Primary (citable) accession number: P42435 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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