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P42434

- NASC_BACSU

UniProt

P42434 - NASC_BACSU

Protein

Assimilatory nitrate reductase catalytic subunit

Gene

nasC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 3 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.

    Cofactori

    Binds 1 4Fe-4S cluster.Curated
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi26 – 261Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi29 – 291Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi33 – 331Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi63 – 631Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. electron carrier activity Source: InterPro
    3. molybdenum ion binding Source: InterPro
    4. nitrate reductase activity Source: UniProtKB-EC
    5. oxidoreductase activity, acting on NAD(P)H Source: RefGenome

    GO - Biological processi

    1. cellular respiration Source: RefGenome
    2. denitrification pathway Source: UniProtKB-UniPathway
    3. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Nitrate assimilation

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciBSUB:BSU03310-MONOMER.
    UniPathwayiUPA00652; UER00706.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Assimilatory nitrate reductase catalytic subunit (EC:1.7.99.4)
    Gene namesi
    Name:nasC
    Synonyms:narB, nasBB
    Ordered Locus Names:BSU03310
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU03310. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 710710Assimilatory nitrate reductase catalytic subunitPRO_0000063239Add
    BLAST

    Proteomic databases

    PaxDbiP42434.

    Expressioni

    Inductioni

    Positively regulated by TnrA under nitrogen-limited conditions.2 Publications

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU03310.

    Structurei

    3D structure databases

    ProteinModelPortaliP42434.
    SMRiP42434. Positions 15-709.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 77594Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031440.
    KOiK00372.
    OrthoDBiEOG6CVV7G.
    PhylomeDBiP42434.

    Family and domain databases

    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42434-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTERLLRYFR DKQQDVQSEK TYDTQCPFCS MQCKMQLVEQ TIVTRKKYTA    50
    IGIDNPTTQG RLCIKGMNAH QHALNSSRIT RPLLKKNGEF MPVSWEEALN 100
    HIKDQVTMIQ TEHGHDAMAV YGSASITNEE AYLLGKFARV GLQTKYIDYN 150
    GRLCMSAAAT AANQTFGADR GLTNPLSDIP HTRVIILAGT NIAECQPTIM 200
    PYFEKAKENG AYFIAIDPRE TATTKIADLH LKIKPGTDAA LANGLVKIII 250
    DEQLINEDFI QSRTNGFEEL KQHTDSLDLN DIAEQTSVSL VDIRKAAVKF 300
    AKETSGMLFT ARGIEQQTDG TAAVKGFLNM VLITGKIGKP YSGYGAITGQ 350
    GNGQGAREHG QKADQLPGYR SIENEEHRAH IAKVWGIHQD ELPRKGVSAY 400
    EMMEKINDGD IKGLFLMCSN PAVSSPNANL VKKALRRLTF FVAIDLFISE 450
    TAKYADVILP ASSYLEDEGT MTNVEGRVTL REASRPCPGE AKHDWQIICD 500
    LASALGKGRY FSYTSAEDIF NELREASRGG IADYSGISYG RLRREGGIHW 550
    PCPESDHPGT GRLFTESFAH PDQKAALSVI PNEPPVPKEK PTADYPLYLT 600
    TGRVMSHYLT GVQTRKSAAL AARHFESFME IHPQTAATYN IEDRVLVKIE 650
    SPRGSITVRS KLSEQIRKDT VFVPIHWADA QNVNDLIGEA LDPACKMPGF 700
    KVCAVRIIPI 710
    Length:710
    Mass (Da):78,622
    Last modified:June 16, 2009 - v3
    Checksum:iE9DDA80014D47A53
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti391 – 3911E → D in BAA08965. (PubMed:8969502)Curated
    Sequence conflicti391 – 3911E → D in BAA06353. (PubMed:7868621)Curated
    Sequence conflicti402 – 4021M → V in BAA08965. (PubMed:8969502)Curated
    Sequence conflicti402 – 4021M → V in BAA06353. (PubMed:7868621)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50453 Genomic DNA. Translation: BAA08965.1.
    AL009126 Genomic DNA. Translation: CAB12125.2.
    D30689 Genomic DNA. Translation: BAA06353.1.
    PIRiE69665.
    RefSeqiNP_388213.2. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB12125; CAB12125; BSU03310.
    GeneIDi938321.
    KEGGibsu:BSU03310.
    PATRICi18972221. VBIBacSub10457_0339.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50453 Genomic DNA. Translation: BAA08965.1 .
    AL009126 Genomic DNA. Translation: CAB12125.2 .
    D30689 Genomic DNA. Translation: BAA06353.1 .
    PIRi E69665.
    RefSeqi NP_388213.2. NC_000964.3.

    3D structure databases

    ProteinModelPortali P42434.
    SMRi P42434. Positions 15-709.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU03310.

    Proteomic databases

    PaxDbi P42434.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12125 ; CAB12125 ; BSU03310 .
    GeneIDi 938321.
    KEGGi bsu:BSU03310.
    PATRICi 18972221. VBIBacSub10457_0339.

    Organism-specific databases

    GenoListi BSU03310. [Micado ]

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031440.
    KOi K00372.
    OrthoDBi EOG6CVV7G.
    PhylomeDBi P42434.

    Enzyme and pathway databases

    UniPathwayi UPA00652 ; UER00706 .
    BioCyci BSUB:BSU03310-MONOMER.

    Family and domain databases

    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes."
      Yamane K., Kumano M., Kurita K.
      Microbiology 142:3047-3056(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
      Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
      Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 391 AND 402.
    4. "The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis."
      Ogawa K., Akagawa E., Yamane K., Sun Z.-W., Lacelle M., Zuber P., Nakano M.M.
      J. Bacteriol. 177:1409-1413(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-710.
      Strain: 168.
    5. "Purification and in vitro activities of the Bacillus subtilis TnrA transcription factor."
      Wray L.V. Jr., Zalieckas J.M., Fisher S.H.
      J. Mol. Biol. 300:29-40(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY TNRA.
      Strain: 168.
    6. "Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box."
      Yoshida K., Yamaguchi H., Kinehara M., Ohki Y.-H., Nakaura Y., Fujita Y.
      Mol. Microbiol. 49:157-165(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY TNRA.

    Entry informationi

    Entry nameiNASC_BACSU
    AccessioniPrimary (citable) accession number: P42434
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 112 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3