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Protein

Assimilatory nitrate reductase catalytic subunit

Gene

nasC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: nitrate reduction (denitrification)

This protein is involved in step 1 of the subpathway that synthesizes dinitrogen from nitrate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Assimilatory nitrate reductase catalytic subunit (nasC)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway nitrate reduction (denitrification), which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dinitrogen from nitrate, the pathway nitrate reduction (denitrification) and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi26Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi29Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi33Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi63Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciBSUB:BSU03310-MONOMER
UniPathwayiUPA00652; UER00706

Names & Taxonomyi

Protein namesi
Recommended name:
Assimilatory nitrate reductase catalytic subunit (EC:1.7.-.-)
Gene namesi
Name:nasC
Synonyms:narB, nasBB
Ordered Locus Names:BSU03310
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000632391 – 710Assimilatory nitrate reductase catalytic subunitAdd BLAST710

Proteomic databases

PaxDbiP42434
PRIDEiP42434

Expressioni

Inductioni

Positively regulated by TnrA under nitrogen-limited conditions.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001843

Structurei

3D structure databases

ProteinModelPortaliP42434
SMRiP42434
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 774Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST59

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031440
InParanoidiP42434
KOiK00372
OMAiTQHWRQQ
PhylomeDBiP42434

Family and domain databases

InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR006655 Mopterin_OxRdtase_prok_CS
IPR027467 MopterinOxRdtase_cofactor_BS
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS00490 MOLYBDOPTERIN_PROK_2, 1 hit

Sequencei

Sequence statusi: Complete.

P42434-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERLLRYFR DKQQDVQSEK TYDTQCPFCS MQCKMQLVEQ TIVTRKKYTA
60 70 80 90 100
IGIDNPTTQG RLCIKGMNAH QHALNSSRIT RPLLKKNGEF MPVSWEEALN
110 120 130 140 150
HIKDQVTMIQ TEHGHDAMAV YGSASITNEE AYLLGKFARV GLQTKYIDYN
160 170 180 190 200
GRLCMSAAAT AANQTFGADR GLTNPLSDIP HTRVIILAGT NIAECQPTIM
210 220 230 240 250
PYFEKAKENG AYFIAIDPRE TATTKIADLH LKIKPGTDAA LANGLVKIII
260 270 280 290 300
DEQLINEDFI QSRTNGFEEL KQHTDSLDLN DIAEQTSVSL VDIRKAAVKF
310 320 330 340 350
AKETSGMLFT ARGIEQQTDG TAAVKGFLNM VLITGKIGKP YSGYGAITGQ
360 370 380 390 400
GNGQGAREHG QKADQLPGYR SIENEEHRAH IAKVWGIHQD ELPRKGVSAY
410 420 430 440 450
EMMEKINDGD IKGLFLMCSN PAVSSPNANL VKKALRRLTF FVAIDLFISE
460 470 480 490 500
TAKYADVILP ASSYLEDEGT MTNVEGRVTL REASRPCPGE AKHDWQIICD
510 520 530 540 550
LASALGKGRY FSYTSAEDIF NELREASRGG IADYSGISYG RLRREGGIHW
560 570 580 590 600
PCPESDHPGT GRLFTESFAH PDQKAALSVI PNEPPVPKEK PTADYPLYLT
610 620 630 640 650
TGRVMSHYLT GVQTRKSAAL AARHFESFME IHPQTAATYN IEDRVLVKIE
660 670 680 690 700
SPRGSITVRS KLSEQIRKDT VFVPIHWADA QNVNDLIGEA LDPACKMPGF
710
KVCAVRIIPI
Length:710
Mass (Da):78,622
Last modified:June 16, 2009 - v3
Checksum:iE9DDA80014D47A53
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti391E → D in BAA08965 (PubMed:8969502).Curated1
Sequence conflicti391E → D in BAA06353 (PubMed:7868621).Curated1
Sequence conflicti402M → V in BAA08965 (PubMed:8969502).Curated1
Sequence conflicti402M → V in BAA06353 (PubMed:7868621).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50453 Genomic DNA Translation: BAA08965.1
AL009126 Genomic DNA Translation: CAB12125.2
D30689 Genomic DNA Translation: BAA06353.1
PIRiE69665
RefSeqiNP_388213.2, NC_000964.3
WP_003246484.1, NZ_JNCM01000030.1

Genome annotation databases

EnsemblBacteriaiCAB12125; CAB12125; BSU03310
GeneIDi938321
KEGGibsu:BSU03310
PATRICifig|224308.179.peg.345

Similar proteinsi

Entry informationi

Entry nameiNASC_BACSU
AccessioniPrimary (citable) accession number: P42434
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 16, 2009
Last modified: May 23, 2018
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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