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Protein

Nitrate transporter

Gene

nasA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May function as a nitrate transporter.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processNitrate assimilation, Transport

Enzyme and pathway databases

BioCyciBSUB:BSU03330-MONOMER.

Protein family/group databases

TCDBi2.A.1.8.2. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate transporter
Gene namesi
Name:nasA
Ordered Locus Names:BSU03330
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei19 – 39HelicalSequence analysisAdd BLAST21
Transmembranei51 – 71HelicalSequence analysisAdd BLAST21
Transmembranei79 – 99HelicalSequence analysisAdd BLAST21
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Transmembranei136 – 156HelicalSequence analysisAdd BLAST21
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Transmembranei277 – 297HelicalSequence analysisAdd BLAST21
Transmembranei300 – 320HelicalSequence analysisAdd BLAST21
Transmembranei333 – 353HelicalSequence analysisAdd BLAST21
Transmembranei364 – 384HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000967351 – 401Nitrate transporterAdd BLAST401

Expressioni

Inductioni

Positively regulated by TnrA under nitrogen-limited conditions.2 Publications

Structurei

3D structure databases

ProteinModelPortaliP42432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000237388.
InParanoidiP42432.
KOiK02575.
OMAiAFMNLMS.
PhylomeDBiP42432.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiView protein in InterPro
IPR011701. MFS.
IPR020846. MFS_dom.
IPR004737. NO3_transporter.
PfamiView protein in Pfam
PF07690. MFS_1. 1 hit.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00886. 2A0108. 1 hit.
PROSITEiView protein in PROSITE
PS50850. MFS. 1 hit.

Sequencei

Sequence statusi: Complete.

P42432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSELKTSG HPLTLLCSFL YFDVSFMIWV MLGALGVYIS QDFGLSPFEK
60 70 80 90 100
GLVVAVPILS GSVFRIILGI LTDRIGPKKT AVIGMLVTMI PLLWGTFGGR
110 120 130 140 150
SLTELYAIGI LLGVAGASFA VALPMASRWY PPHLQGLAMG IAGAGNSGTL
160 170 180 190 200
FATLFGPRLA EQFGWHIVMG IALIPLLIVF ILFVSMAKDS PAQPSPQPLK
210 220 230 240 250
SYLHVFGQKE TWFFCLLYSV TFGGFVGLSS FLSIFFVDQY QLSKIHAGDF
260 270 280 290 300
VTLCVAAGSF FRPVGGLISD RVGGTKVLSV LFVIVALCMA GVSSLPSLSM
310 320 330 340 350
VIVLLFVGMM GLGMGNGAVF QLVPQRFRKE IGMVTGIVGA AGGIGGFFLP
360 370 380 390 400
NILGSLKQMT GTYAIGFITF SCIALLAFAL VLAAGYYWRK SWSAESSPAD

V
Length:401
Mass (Da):42,712
Last modified:June 16, 2009 - v2
Checksum:i1DB7E644EF7FFD58
GO

Sequence cautioni

The sequence BAA06351 differs from that shown. Reason: Frameshift at positions 301, 367 and 371.Curated
The sequence BAA08967 differs from that shown. Reason: Frameshift at positions 301, 367 and 371.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302I → M in BAA06351 (PubMed:7868621).Curated1
Sequence conflicti302I → M in BAA08967 (PubMed:8969502).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30689 Genomic DNA. Translation: BAA06351.1. Frameshift.
D50453 Genomic DNA. Translation: BAA08967.1. Frameshift.
AL009126 Genomic DNA. Translation: CAB12127.2.
PIRiC69665.
RefSeqiNP_388215.2. NC_000964.3.
WP_003246358.1. NZ_JNCM01000030.1.

Genome annotation databases

EnsemblBacteriaiCAB12127; CAB12127; BSU03330.
GeneIDi938326.
KEGGibsu:BSU03330.
PATRICifig|224308.179.peg.347.

Similar proteinsi

Entry informationi

Entry nameiNASA_BACSU
AccessioniPrimary (citable) accession number: P42432
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 16, 2009
Last modified: June 7, 2017
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families