Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P42428 (TFDD2_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chloromuconate cycloisomerase

EC=5.5.1.7
Alternative name(s):
Muconate cycloisomerase II
Gene names
Name:tfdDII
Synonyms:tfdD2
Ordered Locus Names:Reut_D6474
Encoded onPlasmid pJP4 Ref.1 Ref.3
Plasmid pReut1 Ref.2
OrganismCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134)) [Complete proteome] [HAMAP]
Taxonomic identifier264198 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

Cofactor

Manganese By similarity.

Pathway

Aromatic compound metabolism; 3-chlorocatechol degradation.

Sequence similarities

Belongs to the mandelate racemase/muconate lactonizing enzyme family.

Sequence caution

The sequence AAA65064.1 differs from that shown. Reason: Frameshift at positions 82 and 132.

The sequence AAZ65772.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Chloromuconate cycloisomerase
PRO_0000171257

Sites

Active site1581Proton acceptor By similarity
Active site3161Proton donor By similarity
Metal binding1871Manganese By similarity
Metal binding2131Manganese By similarity
Metal binding2381Manganese By similarity

Experimental info

Sequence conflict651A → G in AAA65064. Ref.3
Sequence conflict931A → D Ref.3
Sequence conflict178 – 20124LEGKA…TMRAL → TRGQDEPAPSIPTKRGTSRP RCGT in AAA65064. Ref.3
Sequence conflict208 – 2114GVEI → AWKF in AAA65064. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P42428 [UniParc].

Last modified February 1, 2005. Version 2.
Checksum: 7D170C1364D04140

FASTA37240,045
        10         20         30         40         50         60 
MIVDLPLRRI QQFARLGAKH QSSVLIRLHT KGGIVGIGES ITPCGPWWSG DSVEAIQATI 

        70         80         90        100        110        120 
NHYLAPLVVG EPALDASRIM AKLHGRVAGN AFAKAGIEMA LLDAVGKIVD APIHVLLGGR 

       130        140        150        160        170        180 
FRDRLSVAWP LATGDVNQEV DEAFRMLEAG KAGAFKLKMG ALPLAQDLRR ALAIAKELEG 

       190        200        210        220        230        240 
KASLRVDPNE AWDEPTTMRA LAPLEAAGVE IIEQPVARWN LDAMARIHRQ ARSMLLIDEG 

       250        260        270        280        290        300 
VQSLHDASEV VKRAAAGLVS LKIMKTGGMR PARAMADIAN AGGMHVYMGT FLETSIGTAA 

       310        320        330        340        350        360 
NMQLAASIES LPYGGEVIGP LLIEEDLCEV PAVYKEHALW LPEGPGLGIR LDENQVRRFA 

       370 
RASSQRIDRH SA 

« Hide

References

« Hide 'large scale' references
[1]"Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways."
Trefault N., De la Iglesia R., Molina A.M., Manzano M., Ledger T., Perez-Pantoja D., Sanchez M.A., Stuardo M., Gonzalez B.
Environ. Microbiol. 6:655-668(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of plasmid 1 of Ralstonia eutropha JMP134."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JMP134 / LMG 1197.
[3]"Analysis of duplicated gene sequences associated with tfdR and tfdS in Alcaligenes eutrophus JMP134."
Matrubutham U., Harker A.R.
J. Bacteriol. 176:2348-2353(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-234.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY365053 Genomic DNA. Translation: AAR31047.1.
CP000093 Genomic DNA. Translation: AAZ65772.1. Different initiation.
M98445 Genomic DNA. Translation: AAA65064.1. Frameshift.
RefSeqYP_293629.1. NC_007337.1.

3D structure databases

ProteinModelPortalP42428.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264198.Reut_D6474.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ65772; AAZ65772; Reut_D6474.
GeneID3607898.
KEGGreu:Reut_D6474.
PATRIC20225328. VBIRalEut24049_0543.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG4948.
HOGENOMHOG000185904.
KOK01860.
OMATIVRIDS.
OrthoDBEOG6QG8F7.

Enzyme and pathway databases

BioCycCPIN264198:GIW3-6560-MONOMER.
MetaCyc:MONOMER-14413.
UniPathwayUPA00083.

Family and domain databases

Gene3D3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view]
PANTHERPTHR13794. PTHR13794. 1 hit.
PfamPF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsTIGR02534. mucon_cyclo. 1 hit.
PROSITEPS00908. MR_MLE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTFDD2_CUPPJ
AccessionPrimary (citable) accession number: P42428
Secondary accession number(s): Q46M58, Q6UP90
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 1, 2005
Last modified: June 11, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways