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Protein

Chloromuconate cycloisomerase

Gene

tfdDII

Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei158 – 1581Proton acceptorBy similarity
Metal bindingi187 – 1871ManganeseBy similarity
Metal bindingi213 – 2131ManganeseBy similarity
Metal bindingi238 – 2381ManganeseBy similarity
Active sitei316 – 3161Proton donorBy similarity

GO - Molecular functioni

  1. chloromuconate cycloisomerase activity Source: UniProtKB-EC
  2. manganese ion binding Source: InterPro
  3. muconate cycloisomerase activity Source: InterPro

GO - Biological processi

  1. aromatic compound catabolic process Source: UniProtKB-KW
  2. cellular amino acid catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciCPIN264198:GIW3-6560-MONOMER.
MetaCyc:MONOMER-14413.
UniPathwayiUPA00083.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloromuconate cycloisomerase (EC:5.5.1.7)
Alternative name(s):
Muconate cycloisomerase II
Gene namesi
Name:tfdDII
Synonyms:tfdD2
Ordered Locus Names:Reut_D6474
Encoded oniPlasmid pJP42 Publications
Plasmid pReut11 Publication
OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002697 Componenti: Plasmid pReut1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Chloromuconate cycloisomerasePRO_0000171257Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264198.Reut_D6474.

Structurei

3D structure databases

ProteinModelPortaliP42428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4948.
HOGENOMiHOG000185904.
KOiK01860.
OMAiCADIINI.
OrthoDBiEOG6QG8F7.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR02534. mucon_cyclo. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVDLPLRRI QQFARLGAKH QSSVLIRLHT KGGIVGIGES ITPCGPWWSG
60 70 80 90 100
DSVEAIQATI NHYLAPLVVG EPALDASRIM AKLHGRVAGN AFAKAGIEMA
110 120 130 140 150
LLDAVGKIVD APIHVLLGGR FRDRLSVAWP LATGDVNQEV DEAFRMLEAG
160 170 180 190 200
KAGAFKLKMG ALPLAQDLRR ALAIAKELEG KASLRVDPNE AWDEPTTMRA
210 220 230 240 250
LAPLEAAGVE IIEQPVARWN LDAMARIHRQ ARSMLLIDEG VQSLHDASEV
260 270 280 290 300
VKRAAAGLVS LKIMKTGGMR PARAMADIAN AGGMHVYMGT FLETSIGTAA
310 320 330 340 350
NMQLAASIES LPYGGEVIGP LLIEEDLCEV PAVYKEHALW LPEGPGLGIR
360 370
LDENQVRRFA RASSQRIDRH SA
Length:372
Mass (Da):40,045
Last modified:February 1, 2005 - v2
Checksum:i7D170C1364D04140
GO

Sequence cautioni

The sequence AAA65064.1 differs from that shown. Reason: Frameshift at positions 82 and 132. Curated
The sequence AAZ65772.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651A → G in AAA65064 (PubMed:8157603).Curated
Sequence conflicti93 – 931A → D (PubMed:8157603).Curated
Sequence conflicti178 – 20124LEGKA…TMRAL → TRGQDEPAPSIPTKRGTSRP RCGT in AAA65064 (PubMed:8157603).CuratedAdd
BLAST
Sequence conflicti208 – 2114GVEI → AWKF in AAA65064 (PubMed:8157603).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY365053 Genomic DNA. Translation: AAR31047.1.
CP000093 Genomic DNA. Translation: AAZ65772.1. Different initiation.
M98445 Genomic DNA. Translation: AAA65064.1. Frameshift.
RefSeqiYP_009074314.1. NG_034565.1.
YP_293629.1. NC_007337.1.

Genome annotation databases

EnsemblBacteriaiAAZ65772; AAZ65772; Reut_D6474.
KEGGireu:Reut_D6474.
PATRICi20225328. VBIRalEut24049_0543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY365053 Genomic DNA. Translation: AAR31047.1.
CP000093 Genomic DNA. Translation: AAZ65772.1. Different initiation.
M98445 Genomic DNA. Translation: AAA65064.1. Frameshift.
RefSeqiYP_009074314.1. NG_034565.1.
YP_293629.1. NC_007337.1.

3D structure databases

ProteinModelPortaliP42428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264198.Reut_D6474.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ65772; AAZ65772; Reut_D6474.
KEGGireu:Reut_D6474.
PATRICi20225328. VBIRalEut24049_0543.

Phylogenomic databases

eggNOGiCOG4948.
HOGENOMiHOG000185904.
KOiK01860.
OMAiCADIINI.
OrthoDBiEOG6QG8F7.

Enzyme and pathway databases

UniPathwayiUPA00083.
BioCyciCPIN264198:GIW3-6560-MONOMER.
MetaCyc:MONOMER-14413.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR02534. mucon_cyclo. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways."
    Trefault N., De la Iglesia R., Molina A.M., Manzano M., Ledger T., Perez-Pantoja D., Sanchez M.A., Stuardo M., Gonzalez B.
    Environ. Microbiol. 6:655-668(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pJP4
  2. "Complete sequence of plasmid 1 of Ralstonia eutropha JMP134."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JMP134 / LMG 1197.
    Plasmid: pReut1
  3. "Analysis of duplicated gene sequences associated with tfdR and tfdS in Alcaligenes eutrophus JMP134."
    Matrubutham U., Harker A.R.
    J. Bacteriol. 176:2348-2353(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-234.
    Plasmid: pJP4

Entry informationi

Entry nameiTFDD2_CUPPJ
AccessioniPrimary (citable) accession number: P42428
Secondary accession number(s): Q46M58, Q6UP90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 1, 2005
Last modified: April 1, 2015
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.