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Protein

Lon protease 2

Gene

lon2

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner (By similarity).By similarity

Catalytic activityi

Hydrolysis of proteins in presence of ATP.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei445PROSITE-ProRule annotation1
Active sitei488PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi97 – 104ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU28210-MONOMER

Protein family/group databases

MEROPSiS16.005

Names & Taxonomyi

Protein namesi
Recommended name:
Lon protease 2 (EC:3.4.21.53)
Alternative name(s):
ATP-dependent protease La 2
Gene namesi
Name:lon2
Synonyms:lonB, ysxF
Ordered Locus Names:BSU28210
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000761171 – 552Lon protease 2Add BLAST552

Proteomic databases

PaxDbiP42425

Expressioni

Developmental stagei

Restricted to the prespore compartment of sporulating cells.1 Publication

Inductioni

By heat shock.By similarity

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity (By similarity).By similarity

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015411

Structurei

3D structure databases

ProteinModelPortaliP42425
SMRiP42425
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini349 – 535Lon proteolyticPROSITE-ProRule annotationAdd BLAST187

Sequence similaritiesi

Belongs to the peptidase S16 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108IUQ Bacteria
COG1067 LUCA
HOGENOMiHOG000054711
InParanoidiP42425
KOiK04076
OMAiGLYFWNL
PhylomeDBiP42425

Family and domain databases

Gene3Di3.30.230.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR008269 Lon_proteolytic
IPR027065 Lon_Prtase
IPR027417 P-loop_NTPase
IPR008268 Peptidase_S16_AS
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR014251 Spore_LonB
PANTHERiPTHR10046 PTHR10046, 1 hit
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF05362 Lon_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
SSF54211 SSF54211, 1 hit
TIGRFAMsiTIGR02902 spore_lonB, 1 hit
PROSITEiView protein in PROSITE
PS51786 LON_PROTEOLYTIC, 1 hit
PS01046 LON_SER, 1 hit

Sequencei

Sequence statusi: Complete.

P42425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWTGIALFI QLFFGIIIGL YFWNLLKNQR TQKVTIDKES KKEMEQLRKM
60 70 80 90 100
RAISLSEPLS EKVRPKSFKD IVGQEDGIKA LKAALCGPNP QHVIVYGPPG
110 120 130 140 150
VGKTAAARLV LEEAKKHKQS PFKEQAVFVE LDATTARFDE RGIADPLIGS
160 170 180 190 200
VHDPIYQGAG AMGQAGIPQP KQGAVTHAHG GVLFIDEIGE LHPIQMNKML
210 220 230 240 250
KVLEDRKVFL DSAYYSEENT QIPNHIHDIF QNGLPADFRL IGATTRMPNE
260 270 280 290 300
IPPAIRSRCL EVFFRELEKD ELKTVAKTAA DKIEKNISEE GLDLLTSYTR
310 320 330 340 350
NGREAVNMIQ IAAGMAVTEN RKDITIEDIE WVIHSSQLTP KHEQKIGVEP
360 370 380 390 400
QVGIVNGLAV YGPNSGSLLE IEVSVTAAQD KGSINITGIA EEESIGSQSK
410 420 430 440 450
SIRRKSMAKG SVENVLTVLR TMGMKPSDYD IHINFPGGIP IDGPSAGIAM
460 470 480 490 500
AAGIFSAIHK IPIDNTVAMT GEISLNGLVK PIGGVIPKIK AAKQSGAKKV
510 520 530 540 550
IIPYENQQAI LKQIDGIEII AVKTFQEVLD EILVNPPTEQ KPFHIEINKE

SV
Length:552
Mass (Da):60,429
Last modified:October 1, 1996 - v2
Checksum:i313AAF539662A0B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti500V → G in CAA53987 (PubMed:7961402).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18229 Genomic DNA Translation: AAA84744.1
Z75208 Genomic DNA Translation: CAA99539.1
AL009126 Genomic DNA Translation: CAB14781.1
X76424 Genomic DNA Translation: CAA53987.1
PIRiG69652
RefSeqiNP_390699.1, NC_000964.3
WP_004398923.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14781; CAB14781; BSU28210
GeneIDi937484
KEGGibsu:BSU28210
PATRICifig|224308.179.peg.3064

Similar proteinsi

Entry informationi

Entry nameiLON2_BACSU
AccessioniPrimary (citable) accession number: P42425
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health