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P42403 (BGLC_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aryl-phospho-beta-D-glucosidase BglC

EC=3.2.1.86
Alternative name(s):
6-phospho-beta-glucosidase
Gene names
Name:bglC
Synonyms:yckE
Ordered Locus Names:BSU03410
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Is able to catalyze the hydrolysis of aryl-phospho-beta-D-glucosides such as 4-methylumbelliferyl-phospho-beta-D-glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Is not essential for growth on arbutin and salicin as the sole carbon source. Ref.5

Catalytic activity

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Developmental stage

Expressed at a low and constant level during growth, sporulation, and spore germination. Ref.5

Induction

Is not induced by aryl-beta-D-glucosides such as arbutin, salicin or 4-methylumbelliferyl-beta-D-glucopyranoside (MUG). Is not repressed by glucose. Ref.5

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_function6-phospho-beta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477Aryl-phospho-beta-D-glucosidase BglC
PRO_0000063874

Sites

Active site1701Proton donor Potential
Active site3781Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P42403 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 64045E7E4C1A10E4

FASTA47755,140
        10         20         30         40         50         60 
MIHQHPESFP KHFLWGSASA AYQIEGAWNE DGKGPSVWDV FTKIPGKTFK GTNGEIAVDH 

        70         80         90        100        110        120 
YHRFKEDVAL MAEMGLKAYR FSVSWPRVFP KGKGEINEAG LAFYDSLIDE LLSHHIEPVL 

       130        140        150        160        170        180 
TLYHWDLPQA LMDEYGGFES RNIIEDFNHY CITLYKRFGD RVKYWVTLNE QNYNFNHGFI 

       190        200        210        220        230        240 
TAMHPPGVKD RKRFYEANHI AFLANAKAIE SFREYVPEGK IGPSFAYSPA YPLSSHPEDI 

       250        260        270        280        290        300 
LAFENAEEFT NNWWLDMYCW GTYPQIPFRC LEKQGWAPTI EAGDMDLLAK GKPDFVGVNY 

       310        320        330        340        350        360 
YQTITYERNP LDGVSEGKMN TTGQKGTNQE TGIPGVFKTK KNPHLTTSNW DWTIDPIGLR 

       370        380        390        400        410        420 
IGLRRITSRY QLPVFITENG LGEFDKVEDG TVQDDYRIDY LRSHLEQCRQ AISDGVDLIG 

       430        440        450        460        470 
YCSWSFTDLL SWLNGYQKRY GFVYVNRDEE STSDLKRLKK KSFYWYQDVI KTNGESL 

« Hide

References

« Hide 'large scale' references
[1]"A 10 kb nucleotide sequence at the 5' flanking region (32 degrees) of srfAA of the Bacillus subtilis chromosome."
Fujishima Y., Yamane K.
Microbiology 141:277-279(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes."
Yamane K., Kumano M., Kurita K.
Microbiology 142:3047-3056(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"Transformation in Bacillus subtilis: involvement of the 17-kilodalton DNA-entry nuclease and the competence-specific 18-kilodalton protein."
Vosman B., Kuiken G., Kooistra J., Venema G.
J. Bacteriol. 170:3703-3710(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 461-477.
[5]"Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis."
Setlow B., Cabrera-Hernandez A., Cabrera-Martinez R.M., Setlow P.
Arch. Microbiol. 181:60-67(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS AN ARYL-PHOSPHO-BETA-D-GLUCOSIDASE, DEVELOPMENTAL STAGE, INDUCTION, GENE NAME.
Strain: 168 / PS832.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D30762 Genomic DNA. Translation: BAA06429.1.
D50453 Genomic DNA. Translation: BAA08975.1.
AL009126 Genomic DNA. Translation: CAB12135.1.
M21672 Genomic DNA. No translation available.
PIRG69760.
RefSeqNP_388223.1. NC_000964.3.

3D structure databases

ProteinModelPortalP42403.
SMRP42403. Positions 5-473.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU03410.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbP42403.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12135; CAB12135; BSU03410.
GeneID938317.
KEGGbsu:BSU03410.
PATRIC18972245. VBIBacSub10457_0351.

Organism-specific databases

GenoListBSU03410. [Micado]

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088631.
KOK01223.
OMAERNPLDG.
OrthoDBEOG60658J.
ProtClustDBCLSK2752309.

Enzyme and pathway databases

BioCycBSUB:BSU03410-MONOMER.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGLC_BACSU
AccessionPrimary (citable) accession number: P42403
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 13, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList