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P42403

- BGLC_BACSU

UniProt

P42403 - BGLC_BACSU

Protein

Aryl-phospho-beta-D-glucosidase BglC

Gene

bglC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Is able to catalyze the hydrolysis of aryl-phospho-beta-D-glucosides such as 4-methylumbelliferyl-phospho-beta-D-glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Is not essential for growth on arbutin and salicin as the sole carbon source.1 Publication

    Catalytic activityi

    6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei170 – 1701Proton donorSequence Analysis
    Active sitei378 – 3781NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. 6-phospho-beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BioCyciBSUB:BSU03410-MONOMER.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aryl-phospho-beta-D-glucosidase BglC (EC:3.2.1.86)
    Alternative name(s):
    6-phospho-beta-glucosidase
    Gene namesi
    Name:bglC
    Synonyms:yckE
    Ordered Locus Names:BSU03410
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU03410. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 477477Aryl-phospho-beta-D-glucosidase BglCPRO_0000063874Add
    BLAST

    Proteomic databases

    PaxDbiP42403.

    Expressioni

    Developmental stagei

    Expressed at a low and constant level during growth, sporulation, and spore germination.1 Publication

    Inductioni

    Is not induced by aryl-beta-D-glucosides such as arbutin, salicin or 4-methylumbelliferyl-beta-D-glucopyranoside (MUG). Is not repressed by glucose.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU03410.

    Structurei

    3D structure databases

    ProteinModelPortaliP42403.
    SMRiP42403. Positions 5-473.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088631.
    KOiK01223.
    OMAiENGACDN.
    OrthoDBiEOG60658J.
    PhylomeDBiP42403.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42403-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIHQHPESFP KHFLWGSASA AYQIEGAWNE DGKGPSVWDV FTKIPGKTFK    50
    GTNGEIAVDH YHRFKEDVAL MAEMGLKAYR FSVSWPRVFP KGKGEINEAG 100
    LAFYDSLIDE LLSHHIEPVL TLYHWDLPQA LMDEYGGFES RNIIEDFNHY 150
    CITLYKRFGD RVKYWVTLNE QNYNFNHGFI TAMHPPGVKD RKRFYEANHI 200
    AFLANAKAIE SFREYVPEGK IGPSFAYSPA YPLSSHPEDI LAFENAEEFT 250
    NNWWLDMYCW GTYPQIPFRC LEKQGWAPTI EAGDMDLLAK GKPDFVGVNY 300
    YQTITYERNP LDGVSEGKMN TTGQKGTNQE TGIPGVFKTK KNPHLTTSNW 350
    DWTIDPIGLR IGLRRITSRY QLPVFITENG LGEFDKVEDG TVQDDYRIDY 400
    LRSHLEQCRQ AISDGVDLIG YCSWSFTDLL SWLNGYQKRY GFVYVNRDEE 450
    STSDLKRLKK KSFYWYQDVI KTNGESL 477
    Length:477
    Mass (Da):55,140
    Last modified:November 1, 1995 - v1
    Checksum:i64045E7E4C1A10E4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30762 Genomic DNA. Translation: BAA06429.1.
    D50453 Genomic DNA. Translation: BAA08975.1.
    AL009126 Genomic DNA. Translation: CAB12135.1.
    M21672 Genomic DNA. No translation available.
    PIRiG69760.
    RefSeqiNP_388223.1. NC_000964.3.
    WP_003246242.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB12135; CAB12135; BSU03410.
    GeneIDi938317.
    KEGGibsu:BSU03410.
    PATRICi18972245. VBIBacSub10457_0351.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D30762 Genomic DNA. Translation: BAA06429.1 .
    D50453 Genomic DNA. Translation: BAA08975.1 .
    AL009126 Genomic DNA. Translation: CAB12135.1 .
    M21672 Genomic DNA. No translation available.
    PIRi G69760.
    RefSeqi NP_388223.1. NC_000964.3.
    WP_003246242.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali P42403.
    SMRi P42403. Positions 5-473.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU03410.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PaxDbi P42403.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12135 ; CAB12135 ; BSU03410 .
    GeneIDi 938317.
    KEGGi bsu:BSU03410.
    PATRICi 18972245. VBIBacSub10457_0351.

    Organism-specific databases

    GenoListi BSU03410. [Micado ]

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088631.
    KOi K01223.
    OMAi ENGACDN.
    OrthoDBi EOG60658J.
    PhylomeDBi P42403.

    Enzyme and pathway databases

    BioCyci BSUB:BSU03410-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A 10 kb nucleotide sequence at the 5' flanking region (32 degrees) of srfAA of the Bacillus subtilis chromosome."
      Fujishima Y., Yamane K.
      Microbiology 141:277-279(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes."
      Yamane K., Kumano M., Kurita K.
      Microbiology 142:3047-3056(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "Transformation in Bacillus subtilis: involvement of the 17-kilodalton DNA-entry nuclease and the competence-specific 18-kilodalton protein."
      Vosman B., Kuiken G., Kooistra J., Venema G.
      J. Bacteriol. 170:3703-3710(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 461-477.
    5. "Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis."
      Setlow B., Cabrera-Hernandez A., Cabrera-Martinez R.M., Setlow P.
      Arch. Microbiol. 181:60-67(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS AN ARYL-PHOSPHO-BETA-D-GLUCOSIDASE, DEVELOPMENTAL STAGE, INDUCTION, GENE NAME.
      Strain: 168 / PS832.

    Entry informationi

    Entry nameiBGLC_BACSU
    AccessioniPrimary (citable) accession number: P42403
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3